st060225

7-ethoxy-4-methylchromen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1904
Screen concentration 78.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 66595
SMILES CCOC1=CC2=C(C=C1)C(=CC(=O)O2)C
Standardized SMILES CCOc1ccc2C(=CC(=O)Oc2c1)C
Molecular weight 204.2219
ALogP 2.68
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 67.21
% growth inhibition (Hom. pool) 6.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 66595
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.8|P-value:7.25E-5|Clearance:0.23||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CDC8(YJR057W)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CDS1(YBR029C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.1||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC2(YDL209C)|FD-Score:-3.79|P-value:7.54E-5|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DSL1(YNL258C)|FD-Score:3.82|P-value:6.55E-5|Clearance:0.02||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:GCD2(YGR083C)|FD-Score:5.2|P-value:9.87E-8|Clearance:0.53||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:MED6(YHR058C)|FD-Score:-3.98|P-value:3.47E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:NAT2(YGR147C)|FD-Score:-3.34|P-value:4.22E-4|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOP15(YNL110C)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:OST2(YOR103C)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.09||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PIK1(YNL267W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRE4(YFR050C)|FD-Score:3.1|P-value:9.82E-4|Clearance:0.06||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RNA1(YMR235C)|FD-Score:-3.12|P-value:9.05E-4|Clearance:0||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPS20(YHL015W)|FD-Score:4.16|P-value:1.62E-5|Clearance:0.33||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.37||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRN11(YML043C)|FD-Score:-3.28|P-value:5.18E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRN5(YLR141W)|FD-Score:-3.11|P-value:9.35E-4|Clearance:0||SGD DESC:Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p Gene:TIM17(YJL143W)|FD-Score:4.79|P-value:8.27E-7|Clearance:0.53||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:YTH1(YPR107C)|FD-Score:-4.6|P-value:2.09E-6|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACT1(YFL039C)|FD-Score:3.8|P-value:7.25E-5|Clearance:0.23||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CDC8(YJR057W)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CDS1(YBR029C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.1||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC2(YDL209C)|FD-Score:-3.79|P-value:7.54E-5|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DSL1(YNL258C)|FD-Score:3.82|P-value:6.55E-5|Clearance:0.02||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:GCD2(YGR083C)|FD-Score:5.2|P-value:9.87E-8|Clearance:0.53||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:MED6(YHR058C)|FD-Score:-3.98|P-value:3.47E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:NAT2(YGR147C)|FD-Score:-3.34|P-value:4.22E-4|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOP15(YNL110C)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:OST2(YOR103C)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.09||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PIK1(YNL267W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRE4(YFR050C)|FD-Score:3.1|P-value:9.82E-4|Clearance:0.06||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RNA1(YMR235C)|FD-Score:-3.12|P-value:9.05E-4|Clearance:0||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPS20(YHL015W)|FD-Score:4.16|P-value:1.62E-5|Clearance:0.33||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS5(YJR123W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.37||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRN11(YML043C)|FD-Score:-3.28|P-value:5.18E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRN5(YLR141W)|FD-Score:-3.11|P-value:9.35E-4|Clearance:0||SGD DESC:Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p Gene:TIM17(YJL143W)|FD-Score:4.79|P-value:8.27E-7|Clearance:0.53||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:YTH1(YPR107C)|FD-Score:-4.6|P-value:2.09E-6|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 66595
Download HOP data (tab-delimited text)  (excel)
Gene:AIM25(YJR100C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARC1(YGL105W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CCC2(YDR270W)|FD-Score:5.32|P-value:5.13E-8||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CIT3(YPR001W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CSI1(YMR025W)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome Gene:CYS3(YAL012W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-5.68|P-value:6.83E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:4.88|P-value:5.22E-7||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DSE2(YHR143W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EAP1(YKL204W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EMI5(YOL071W)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) Gene:FAP1(YNL023C)|FD-Score:-3.97|P-value:3.64E-5||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FUI1(YBL042C)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GCS1(YDL226C)|FD-Score:6.15|P-value:3.87E-10||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEA1(YJR031C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GEP3(YOR205C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GTR1(YML121W)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GYP7(YDL234C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:HPR1(YDR138W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IES1(YFL013C)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:MAL13(YGR288W)|FD-Score:6.67|P-value:1.24E-11||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MDR1(YGR100W)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MFT1(YML062C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MSH2(YOL090W)|FD-Score:6.94|P-value:1.95E-12||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MUP3(YHL036W)|FD-Score:4|P-value:3.16E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NAM7(YMR080C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NOP12(YOL041C)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NUP188(YML103C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Gene:PET54(YGR222W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX22(YAL055W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:POL32(YJR043C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PTK1(YKL198C)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:PUS7(YOR243C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RIM21(YNL294C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:ROY1(YMR258C)|FD-Score:5.11|P-value:1.61E-7||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPN4(YDL020C)|FD-Score:-4.54|P-value:2.87E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAP4(YGL229C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEG2(YKL105C_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:TEF4(YKL081W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:THI12(YNL332W)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:VMA1(YDL185W)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS45(YGL095C)|FD-Score:5.64|P-value:8.69E-9||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR062C(YBR062C_p)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR292C(YBR292C_d)|FD-Score:4.35|P-value:6.72E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YEL010W(YEL010W_d)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL054C(YFL054C_p)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YJR015W(YJR015W_p)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YKL115C(YKL115C_d)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR434C(YLR434C_d)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML020W(YML020W_p)|FD-Score:5.71|P-value:5.78E-9||SGD DESC:Putative protein of unknown function Gene:YML094C-A(YML094C-A_d)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YOR041C(YOR041C_d)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR111W(YOR111W_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:6.29|P-value:1.62E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL119C-A(YPL119C-A_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YSP2(YDR326C)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ZRG8(YER033C)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ZTA1(YBR046C)|FD-Score:-4.37|P-value:6.24E-6||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:AIM25(YJR100C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARC1(YGL105W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CCC2(YDR270W)|FD-Score:5.32|P-value:5.13E-8||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CIT3(YPR001W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CSI1(YMR025W)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome Gene:CYS3(YAL012W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-5.68|P-value:6.83E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:4.88|P-value:5.22E-7||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DSE2(YHR143W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EAP1(YKL204W)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EMI5(YOL071W)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) Gene:FAP1(YNL023C)|FD-Score:-3.97|P-value:3.64E-5||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FUI1(YBL042C)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GCS1(YDL226C)|FD-Score:6.15|P-value:3.87E-10||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEA1(YJR031C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GEP3(YOR205C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GTR1(YML121W)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GYP7(YDL234C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:HPR1(YDR138W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IES1(YFL013C)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:MAL13(YGR288W)|FD-Score:6.67|P-value:1.24E-11||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MDR1(YGR100W)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MFT1(YML062C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MSH2(YOL090W)|FD-Score:6.94|P-value:1.95E-12||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MUP3(YHL036W)|FD-Score:4|P-value:3.16E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NAM7(YMR080C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NOP12(YOL041C)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NUP188(YML103C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Gene:PET54(YGR222W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX22(YAL055W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:POL32(YJR043C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PTK1(YKL198C)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:PUS7(YOR243C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RIM21(YNL294C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:ROY1(YMR258C)|FD-Score:5.11|P-value:1.61E-7||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPN4(YDL020C)|FD-Score:-4.54|P-value:2.87E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAP4(YGL229C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEG2(YKL105C_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:TEF4(YKL081W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:THI12(YNL332W)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:VMA1(YDL185W)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS45(YGL095C)|FD-Score:5.64|P-value:8.69E-9||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR062C(YBR062C_p)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR292C(YBR292C_d)|FD-Score:4.35|P-value:6.72E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YEL010W(YEL010W_d)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL054C(YFL054C_p)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YJR015W(YJR015W_p)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YKL115C(YKL115C_d)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR434C(YLR434C_d)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML020W(YML020W_p)|FD-Score:5.71|P-value:5.78E-9||SGD DESC:Putative protein of unknown function Gene:YML094C-A(YML094C-A_d)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YOR041C(YOR041C_d)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR111W(YOR111W_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:6.29|P-value:1.62E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL119C-A(YPL119C-A_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YSP2(YDR326C)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ZRG8(YER033C)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ZTA1(YBR046C)|FD-Score:-4.37|P-value:6.24E-6||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR083C5.209.87E-80.53GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YJL143W4.798.27E-70.53TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YBR029C4.261.03E-50.10CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YHL015W4.161.62E-50.33RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YNL258C3.826.55E-50.02DSL1Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
YFL039C3.807.25E-50.23ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YJR123W3.571.81E-40.36RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YNL110C3.206.85E-40.01NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YOR103C3.197.21E-40.09OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YFR050C3.109.82E-40.06PRE4Beta 7 subunit of the 20S proteasome
YLL037W_d3.030.001210.00YLL037W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
YGL030W3.030.001230.18RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YDL212W2.850.002170.05SHR3Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
YIL004C2.800.002540.03BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YLR076C_d2.770.002820.02YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL090W6.941.95E-12MSH2Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
YGR288W6.671.24E-11MAL13MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C
YKL126W6.291.62E-10YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDL226C6.153.87E-10GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YML020W_p5.715.78E-9YML020W_pPutative protein of unknown function
YGL095C5.648.69E-9VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YDR270W5.325.13E-8CCC2Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
YMR258C5.111.61E-7ROY1GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
YGR092W4.885.22E-7DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YKL198C4.523.11E-6PTK1Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein
YKL115C_d4.444.58E-6YKL115C_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
YBR292C_d4.356.72E-6YBR292C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene
YAL012W4.251.08E-5CYS3Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
YLR370C4.161.56E-5ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YML094C-A_d4.082.21E-5YML094C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO

GO enrichment analysis for SGTC_1904
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1491.08E-30SGTC_1902st060212 70.5 μMTimTec (Natural product derivative library)6889440.571429
0.1462.67E-29SGTC_1901st060216 58.6 μMTimTec (Natural product derivative library)6887920.26
0.1449.78E-29SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0487805
0.1232.21E-21SGTC_9011112-0062 60.1 μMChemDiv (Drug-like library)19084570.0634921
0.1063.24E-16SGTC_13081187-1617 41.4 μMChemDiv (Drug-like library)28825700.126984fatty acid desaturase (OLE1)
0.1041.00E-15SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0722892
0.1025.82E-15SGTC_2675theaflavin digallate 100.0 μMMicrosource (Natural product library)4673200.108434iron homeostasis
0.0961.95E-13SGTC_1900st060200 72.8 μMTimTec (Natural product derivative library)6889450.783784
0.0953.44E-13SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.056338endomembrane recycling
0.0938.38E-13SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.117647fatty acid desaturase (OLE1)
0.0921.88E-12SGTC_8680527-0199 213.0 μMChemDiv (Drug-like library)28494500.0714286fatty acid desaturase (OLE1)
0.0912.04E-12SGTC_31889110789 49.5 μMChembridge (Drug-like library)8492170.267857iron homeostasis
0.0912.59E-12SGTC_2666tannic acid 100.0 μMMicrosource (Natural product library)161342670.0972222iron homeostasis
0.0903.92E-12SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.111111fatty acid desaturase (OLE1)
0.0896.62E-12SGTC_3740880-0231 82.3 μMChemDiv (Drug-like library)68335870.0632911iron homeostasis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1900st06020072.8 μM0.783784688945TimTec (Natural product derivative library)204.221882.67803
SGTC_15447-methoxy-4-methylcoumarin105 μM0.702703390807TimTec (Pure natural product library)190.19532.32903
SGTC_1909st06083751.5 μM0.651163678937TimTec (Natural product derivative library)266.291263.7103
SGTC_1912st06020990.8 μM0.619048688787TimTec (Natural product derivative library)220.221281.9204
SGTC_1841st05414870 μM0.617021675442TimTec (Natural product derivative library)285.29462.79505
SGTC_1902st06021270.5 μM0.571429688944TimTec (Natural product derivative library)204.221882.42203
SGTC_1905st06020784.5 μM0.5555561948547TimTec (Natural product derivative library)216.232582.94603
SGTC_7844579-0127173 μM0.5555561948547ChemDiv (Drug-like library)216.232582.94603
SGTC_1903st06021178.3 μM0.511111688960TimTec (Natural product derivative library)220.221281.9204
SGTC_1976st07060963.5 μM0.509804682556TimTec (Natural product derivative library)314.719844.44204