st057770

(E)-3-(4-hydroxy-3-methoxyphenyl)-1-(2-hydroxy-5-methylphenyl)prop-2-en-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1925
Screen concentration 10.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 5989122
SMILES CC1=CC(=C(C=C1)O)C(=O)C=CC2=CC(=C(C=C2)O)OC
Standardized SMILES COc1cc(C=CC(=O)c2cc(C)ccc2O)ccc1O
Molecular weight 284.3065
ALogP 3.69
H-bond donor count 2
H-bond acceptor count 4
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.14
% growth inhibition (Hom. pool) 5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5989122
Download HIP data (tab-delimited text)  (excel)
Gene:CDC42(YLR229C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.01||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:ERG29(YMR134W_p)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:ESF2(YNR054C)|FD-Score:-3.66|P-value:1.27E-4|Clearance:0||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:HRT1(YOL133W)|FD-Score:-6.79|P-value:5.56E-12|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MIA40(YKL195W)|FD-Score:3.81|P-value:6.85E-5|Clearance:0.22||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MPS2(YGL075C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.04||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NAN1(YPL126W)|FD-Score:-3.44|P-value:2.91E-4|Clearance:0||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NAT2(YGR147C)|FD-Score:3.2|P-value:6.87E-4|Clearance:0.02||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PRT1(YOR361C)|FD-Score:3.22|P-value:6.40E-4|Clearance:0.02||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RPB11(YOL005C)|FD-Score:4.27|P-value:9.77E-6|Clearance:0.25||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPL30(YGL030W)|FD-Score:6|P-value:9.76E-10|Clearance:1.73||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:SOG2(YOR353C)|FD-Score:4.02|P-value:2.88E-5|Clearance:0.05||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:SSU72(YNL222W)|FD-Score:-3.24|P-value:6.02E-4|Clearance:0||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:TOM22(YNL131W)|FD-Score:3.98|P-value:3.50E-5|Clearance:0.04||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRM112(YNR046W)|FD-Score:-3.35|P-value:4.08E-4|Clearance:0||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YNL114C(YNL114C_d)|FD-Score:3.94|P-value:4.16E-5|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YNL150W(YNL150W_d)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPL142C(YPL142C_d)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:CDC42(YLR229C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.01||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:ERG29(YMR134W_p)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:ESF2(YNR054C)|FD-Score:-3.66|P-value:1.27E-4|Clearance:0||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:HRT1(YOL133W)|FD-Score:-6.79|P-value:5.56E-12|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MIA40(YKL195W)|FD-Score:3.81|P-value:6.85E-5|Clearance:0.22||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MPS2(YGL075C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.04||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NAN1(YPL126W)|FD-Score:-3.44|P-value:2.91E-4|Clearance:0||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NAT2(YGR147C)|FD-Score:3.2|P-value:6.87E-4|Clearance:0.02||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PRT1(YOR361C)|FD-Score:3.22|P-value:6.40E-4|Clearance:0.02||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RPB11(YOL005C)|FD-Score:4.27|P-value:9.77E-6|Clearance:0.25||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPL30(YGL030W)|FD-Score:6|P-value:9.76E-10|Clearance:1.73||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:SOG2(YOR353C)|FD-Score:4.02|P-value:2.88E-5|Clearance:0.05||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:SSU72(YNL222W)|FD-Score:-3.24|P-value:6.02E-4|Clearance:0||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:TOM22(YNL131W)|FD-Score:3.98|P-value:3.50E-5|Clearance:0.04||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRM112(YNR046W)|FD-Score:-3.35|P-value:4.08E-4|Clearance:0||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YNL114C(YNL114C_d)|FD-Score:3.94|P-value:4.16E-5|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YNL150W(YNL150W_d)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPL142C(YPL142C_d)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5989122
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADE8(YDR408C)|FD-Score:6.03|P-value:8.31E-10||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:APM1(YPL259C)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARA1(YBR149W)|FD-Score:-7.2|P-value:2.91E-13||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:AVL9(YLR114C)|FD-Score:4.92|P-value:4.41E-7||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BEM4(YPL161C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNI1(YNL271C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD14(YAR014C)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CAF20(YOR276W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CBR1(YIL043C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CIT1(YNR001C)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CYS3(YAL012W)|FD-Score:4.45|P-value:4.24E-6||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:ECM3(YOR092W)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:EPT1(YHR123W)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:FAT1(YBR041W)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FLO11(YIR019C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FMT1(YBL013W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:GRX6(YDL010W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GSY2(YLR258W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GTF1(YGR102C)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GTO1(YGR154C)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HOM2(YDR158W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:KTI12(YKL110C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LAA1(YJL207C)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LCL1(YPL056C_p)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LDB19(YOR322C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LYS20(YDL182W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication Gene:MAL32(YBR299W)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MNN11(YJL183W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MOD5(YOR274W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRC1(YCL061C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MTC6(YHR151C)|FD-Score:-3.81|P-value:6.93E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MUD1(YBR119W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MUP3(YHL036W)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:MYO4(YAL029C)|FD-Score:5.05|P-value:2.17E-7||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:PGM2(YMR105C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress Gene:PHO88(YBR106W)|FD-Score:4.91|P-value:4.61E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RGP1(YDR137W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM13(YMR154C)|FD-Score:4.3|P-value:8.61E-6||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RPL20B(YOR312C)|FD-Score:-4.11|P-value:1.94E-5||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS16A(YMR143W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRG9(YNL213C)|FD-Score:-4.87|P-value:5.45E-7||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRT1(YBL048W_d)|FD-Score:4.99|P-value:3.05E-7||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SAC7(YDR389W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAY1(YGR263C)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SEC28(YIL076W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SEC72(YLR292C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHM1(YBR263W)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:SIN3(YOL004W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SLH1(YGR271W)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLI15(YBR156C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SNO1(YMR095C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SRV2(YNL138W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SVS1(YPL163C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TBS1(YBR150C)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TIF2(YJL138C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TOF2(YKR010C)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:14.6|P-value:9.84E-49||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:ULA1(YPL003W)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VPS24(YKL041W)|FD-Score:7.49|P-value:3.47E-14||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YBL065W(YBL065W_d)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YEA6(YEL006W)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YJL068C(YJL068C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJL120W(YJL120W_d)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL147C(YKL147C_d)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YLR225C(YLR225C_p)|FD-Score:-3.87|P-value:5.44E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR085W(YMR085W_p)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR194C-A(YMR194C-A_d)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL034W(YNL034W_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YPC1(YBR183W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:AAC1(YMR056C)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADE8(YDR408C)|FD-Score:6.03|P-value:8.31E-10||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:APM1(YPL259C)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARA1(YBR149W)|FD-Score:-7.2|P-value:2.91E-13||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:AVL9(YLR114C)|FD-Score:4.92|P-value:4.41E-7||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BEM4(YPL161C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNI1(YNL271C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD14(YAR014C)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CAF20(YOR276W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CBR1(YIL043C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CIT1(YNR001C)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CYS3(YAL012W)|FD-Score:4.45|P-value:4.24E-6||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:ECM3(YOR092W)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:EPT1(YHR123W)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:FAT1(YBR041W)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FLO11(YIR019C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FMT1(YBL013W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:GRX6(YDL010W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GSY2(YLR258W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GTF1(YGR102C)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GTO1(YGR154C)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HOM2(YDR158W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:KTI12(YKL110C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LAA1(YJL207C)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LCL1(YPL056C_p)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LDB19(YOR322C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LYS20(YDL182W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication Gene:MAL32(YBR299W)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MNN11(YJL183W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MOD5(YOR274W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRC1(YCL061C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MTC6(YHR151C)|FD-Score:-3.81|P-value:6.93E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MUD1(YBR119W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MUP3(YHL036W)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:MYO4(YAL029C)|FD-Score:5.05|P-value:2.17E-7||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:PGM2(YMR105C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress Gene:PHO88(YBR106W)|FD-Score:4.91|P-value:4.61E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RGP1(YDR137W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM13(YMR154C)|FD-Score:4.3|P-value:8.61E-6||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RPL20B(YOR312C)|FD-Score:-4.11|P-value:1.94E-5||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS16A(YMR143W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRG9(YNL213C)|FD-Score:-4.87|P-value:5.45E-7||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRT1(YBL048W_d)|FD-Score:4.99|P-value:3.05E-7||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SAC7(YDR389W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAY1(YGR263C)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SEC28(YIL076W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SEC72(YLR292C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHM1(YBR263W)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:SIN3(YOL004W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SLH1(YGR271W)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLI15(YBR156C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SNO1(YMR095C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SRV2(YNL138W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SVS1(YPL163C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TBS1(YBR150C)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TIF2(YJL138C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TOF2(YKR010C)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:14.6|P-value:9.84E-49||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:ULA1(YPL003W)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VPS24(YKL041W)|FD-Score:7.49|P-value:3.47E-14||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YBL065W(YBL065W_d)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YEA6(YEL006W)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YJL068C(YJL068C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJL120W(YJL120W_d)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL147C(YKL147C_d)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YLR225C(YLR225C_p)|FD-Score:-3.87|P-value:5.44E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR085W(YMR085W_p)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR194C-A(YMR194C-A_d)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL034W(YNL034W_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YPC1(YBR183W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL030W6.009.76E-101.73RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YOL005C4.279.77E-60.25RPB11RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
YOR353C4.022.88E-50.05SOG2Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
YNL131W3.983.50E-50.04TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YNL114C_d3.944.16E-50.12YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YKL195W3.816.85E-50.21MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YDL196W_d3.601.60E-40.05YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YGL075C3.551.94E-40.04MPS2Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
YLR229C3.512.22E-40.01CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YNL150W_d3.502.29E-40.28YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YOR361C3.226.40E-40.02PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YGR147C3.206.87E-40.02NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YPL142C_d3.187.46E-40.09YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YPR085C3.080.001020.03ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YGL111W3.050.001150.06NSA1Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A14.609.84E-49TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YKL041W7.493.47E-14VPS24One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
YDR408C6.038.31E-10ADE8Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
YMR056C5.571.26E-8AAC1Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
YNR001C5.141.36E-7CIT1Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
YAL029C5.052.17E-7MYO4Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication
YBL048W_d4.993.05E-7RRT1_dIdentified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YLR114C4.924.41E-7AVL9Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
YBR106W4.914.61E-7PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YAR014C4.856.17E-7BUD14Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
YBR183W4.711.26E-6YPC1Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication
YMR194C-A_d4.572.39E-6YMR194C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR299W4.562.54E-6MAL32Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
YAL012W4.454.24E-6CYS3Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
YHL036W4.415.11E-6MUP3Low affinity methionine permease, similar to Mup1p

GO enrichment analysis for SGTC_1925
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2982.60E-121SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.102564TSC3-RPN4
0.2921.97E-116SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0298507TSC3-RPN4
0.2783.61E-105SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.290909TSC3-RPN4
0.2753.53E-103SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0806452TSC3-RPN4
0.2759.69E-103SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0821918TSC3-RPN4
0.2654.53E-95SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.104478TSC3-RPN4
0.2622.03E-93SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.1125TSC3-RPN4
0.2553.27E-88SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.105263
0.2554.12E-88SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.20339TSC3-RPN4
0.2509.64E-85SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.232143TSC3-RPN4
0.2488.01E-84SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0694444TSC3-RPN4
0.2462.44E-82SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.112903TSC3-RPN4
0.2449.12E-81SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.5TSC3-RPN4
0.2404.47E-78SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.25
0.2332.38E-73SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.184615TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_25792',5'-dihydroxy-4-methoxychalcone13.23 μM0.5744685355888Microsource (Natural product library)270.279963.20124
SGTC_11730443-0020122 μM0.55331295ChemDiv (Drug-like library)254.280563.44313TSC3-RPN4
SGTC_25992',3-dihydroxy-4,4',6'-trimethoxychalcone100 μM0.4807695379071Microsource (Natural product library)330.331923.16826
SGTC_25772',4'-dihydroxy-4-methoxychalcone15.32 μM0.485711223Microsource (Natural product library)270.279963.20124
SGTC_15852',4'-dihydroxychalcone10.4 μM0.4255325357218TimTec (Pure natural product library)240.253983.21823
SGTC_25732',4'-dihydroxychalcone15.04 μM0.4255325357218TimTec (Pure natural product library)240.253983.21823
SGTC_25742',4'-dihydroxychalcone14.64 μM0.4255325357218TimTec (Pure natural product library)240.253983.21823
SGTC_26602',4'-dihydroxychalcone17.14 μM0.4255325357218TimTec (Pure natural product library)240.253983.21823
SGTC_26682',4'-dihydroxychalcone15.08 μM0.4255325357218TimTec (Pure natural product library)240.253983.21823
SGTC_2565isoliquiritigenin100 μM0.425532638278Microsource (Natural product library)256.253382.97534
SGTC_3041310-029897 μM0.4117651550234ChemDiv (Drug-like library)366.407084.6225cell wall
SGTC_8600443-001625.1 μM0.4081635337942ChemDiv (Drug-like library)242.2450433.66513
SGTC_2586curcumin80 μM0.389836610332ICCB bioactive library368.37993.55426iron homeostasis
SGTC_444curcumin90.4 μM0.389836610332ICCB bioactive library368.37993.55426iron homeostasis
SGTC_12201838-0075193 μM0.389835529645ChemDiv (Drug-like library)379.20533.15625