4'-methoxychalcone

(E)-1-(4-methoxyphenyl)-3-phenylprop-2-en-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1928
Screen concentration 40.3 μM
Source TimTec (Natural product derivative library)
PubChem CID 641818
SMILES COC1=CC=C(C=C1)C(=O)C=CC2=CC=CC=C2
Standardized SMILES COc1ccc(cc1)C(=O)C=Cc2ccccc2
Molecular weight 238.2812
ALogP 3.69
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.33
% growth inhibition (Hom. pool) 3.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 641818
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.62||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALG2(YGL065C)|FD-Score:3.88|P-value:5.32E-5|Clearance:0.62||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:CDC25(YLR310C)|FD-Score:3.75|P-value:8.70E-5|Clearance:0.62||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CHS2(YBR038W)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:DAD2(YKR083C)|FD-Score:-3.44|P-value:2.96E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:EBP2(YKL172W)|FD-Score:4.22|P-value:1.24E-5|Clearance:0.62||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:ESF2(YNR054C)|FD-Score:-4.65|P-value:1.62E-6|Clearance:0||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:FIP1(YJR093C)|FD-Score:3.98|P-value:3.42E-5|Clearance:0.62||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:KAP95(YLR347C)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KRE5(YOR336W)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:KRE9(YJL174W)|FD-Score:-3.17|P-value:7.67E-4|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:NUP82(YJL061W)|FD-Score:-3.33|P-value:4.37E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PDI1(YCL043C)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.62||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:POL31(YJR006W)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:RHO1(YPR165W)|FD-Score:4.67|P-value:1.48E-6|Clearance:0.62||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPC82(YPR190C)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.62||SGD DESC:RNA polymerase III subunit C82 Gene:RSC58(YLR033W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:TOM40(YMR203W)|FD-Score:-3.46|P-value:2.67E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:VRG4(YGL225W)|FD-Score:4|P-value:3.15E-5|Clearance:0.62||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:VTI1(YMR197C)|FD-Score:-3.73|P-value:9.63E-5|Clearance:0||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YTH1(YPR107C)|FD-Score:-4.09|P-value:2.12E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACT1(YFL039C)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.62||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALG2(YGL065C)|FD-Score:3.88|P-value:5.32E-5|Clearance:0.62||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:CDC25(YLR310C)|FD-Score:3.75|P-value:8.70E-5|Clearance:0.62||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CHS2(YBR038W)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:DAD2(YKR083C)|FD-Score:-3.44|P-value:2.96E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:EBP2(YKL172W)|FD-Score:4.22|P-value:1.24E-5|Clearance:0.62||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:ESF2(YNR054C)|FD-Score:-4.65|P-value:1.62E-6|Clearance:0||SGD DESC:Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Gene:FIP1(YJR093C)|FD-Score:3.98|P-value:3.42E-5|Clearance:0.62||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:KAP95(YLR347C)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KRE5(YOR336W)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:KRE9(YJL174W)|FD-Score:-3.17|P-value:7.67E-4|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:NUP82(YJL061W)|FD-Score:-3.33|P-value:4.37E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PDI1(YCL043C)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.62||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:POL31(YJR006W)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:RHO1(YPR165W)|FD-Score:4.67|P-value:1.48E-6|Clearance:0.62||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPC82(YPR190C)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.62||SGD DESC:RNA polymerase III subunit C82 Gene:RSC58(YLR033W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:TOM40(YMR203W)|FD-Score:-3.46|P-value:2.67E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:VRG4(YGL225W)|FD-Score:4|P-value:3.15E-5|Clearance:0.62||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:VTI1(YMR197C)|FD-Score:-3.73|P-value:9.63E-5|Clearance:0||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YTH1(YPR107C)|FD-Score:-4.09|P-value:2.12E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 641818
Download HOP data (tab-delimited text)  (excel)
Gene:ARO1(YDR127W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG17(YLR423C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG8(YBL078C)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:CKI1(YLR133W)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLB1(YGR108W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:DBP7(YKR024C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DPB3(YBR278W)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ECM18(YDR125C)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FYV12(YOR183W_p)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GMH1(YKR030W)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GSY2(YLR258W)|FD-Score:-4.31|P-value:8.01E-6||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HAL9(YOL089C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HAP5(YOR358W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HSP42(YDR171W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:HSV2(YGR223C)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HXK1(YFR053C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:KDX1(YKL161C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:KIN2(YLR096W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KSS1(YGR040W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LTE1(YAL024C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDR1(YGR100W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MRPL16(YBL038W)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MSS51(YLR203C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NTC20(YBR188C)|FD-Score:-3.9|P-value:4.74E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:PRY2(YKR013W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAD5(YLR032W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:REI1(YBR267W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RHR2(YIL053W)|FD-Score:-4.4|P-value:5.36E-6||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RMD6(YEL072W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein required for sporulation Gene:RPL15B(YMR121C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPS21B(YJL136C)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:SAM1(YLR180W)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SNX4(YJL036W)|FD-Score:-3.81|P-value:6.93E-5||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:STE13(YOR219C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:SWI6(YLR182W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TFB5(YDR079C-A)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TIM13(YGR181W)|FD-Score:5.04|P-value:2.29E-7||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TOM7(YNL070W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPO4(YOR273C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:9.06|P-value:6.70E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS45(YGL095C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAR035C-A(YAR035C-A_p)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR285W(YBR285W_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCR064C(YCR064C_d)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YCR101C(YCR101C_p)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR246W-A(YDR246W-A_p)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER186C(YER186C_p)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:Putative protein of unknown function Gene:YKE4(YIL023C)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YLR412C-A(YLR412C-A_p)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Putative protein of unknown function Gene:YMR173W-A(YMR173W-A_d)|FD-Score:-4.33|P-value:7.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL319W(YNL319W_d)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YPT11(YNL304W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:ARO1(YDR127W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG17(YLR423C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG8(YBL078C)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:CKI1(YLR133W)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLB1(YGR108W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:DBP7(YKR024C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DPB3(YBR278W)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ECM18(YDR125C)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FYV12(YOR183W_p)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GMH1(YKR030W)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GSY2(YLR258W)|FD-Score:-4.31|P-value:8.01E-6||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HAL9(YOL089C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HAP5(YOR358W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HSP42(YDR171W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:HSV2(YGR223C)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HXK1(YFR053C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:KDX1(YKL161C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:KIN2(YLR096W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KSS1(YGR040W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LTE1(YAL024C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDR1(YGR100W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MRPL16(YBL038W)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MSS51(YLR203C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NTC20(YBR188C)|FD-Score:-3.9|P-value:4.74E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:PRY2(YKR013W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAD5(YLR032W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:REI1(YBR267W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RHR2(YIL053W)|FD-Score:-4.4|P-value:5.36E-6||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RMD6(YEL072W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein required for sporulation Gene:RPL15B(YMR121C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPS21B(YJL136C)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:SAM1(YLR180W)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SNX4(YJL036W)|FD-Score:-3.81|P-value:6.93E-5||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:STE13(YOR219C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:SWI6(YLR182W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TFB5(YDR079C-A)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TIM13(YGR181W)|FD-Score:5.04|P-value:2.29E-7||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TOM7(YNL070W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPO4(YOR273C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:9.06|P-value:6.70E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS45(YGL095C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAR035C-A(YAR035C-A_p)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR285W(YBR285W_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCR064C(YCR064C_d)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YCR101C(YCR101C_p)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR246W-A(YDR246W-A_p)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER186C(YER186C_p)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:Putative protein of unknown function Gene:YKE4(YIL023C)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YLR412C-A(YLR412C-A_p)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Putative protein of unknown function Gene:YMR173W-A(YMR173W-A_d)|FD-Score:-4.33|P-value:7.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL319W(YNL319W_d)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YPT11(YNL304W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR165W4.671.48E-60.62RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YKL172W4.221.24E-50.62EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YGL225W4.003.15E-50.62VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YJR093C3.983.42E-50.62FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YPR190C3.963.80E-50.62RPC82RNA polymerase III subunit C82
YGL065C3.885.32E-50.62ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YLR310C3.758.70E-50.62CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YFL039C3.601.58E-40.62ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YCL043C3.542.03E-40.62PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YLR230W_d2.910.001790.12YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YBR167C2.790.002620.02POP7Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR166C2.770.002820.00SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YDR208W2.770.002830.01MSS4Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
YDR082W2.760.002900.03STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YDR361C2.730.003190.11BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A9.066.70E-20TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YLR133W5.131.44E-7CKI1Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
YGR181W5.042.29E-7TIM13Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YER186C_p4.552.70E-6YER186C_pPutative protein of unknown function
YBL038W4.444.46E-6MRPL16Mitochondrial ribosomal protein of the large subunit
YKR013W4.251.08E-5PRY2Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication
YFR053C4.191.40E-5HXK1Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
YMR021C4.151.65E-5MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YLR412C-A_p4.111.94E-5YLR412C-A_pPutative protein of unknown function
YAR035C-A_p4.022.93E-5YAR035C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis
YGL095C3.944.05E-5VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YCR101C_p3.924.37E-5YCR101C_pPutative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene
YNL319W_d3.924.37E-5YNL319W_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14
YGR108W3.885.29E-5CLB1B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
YKR030W3.875.38E-5GMH1Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting

GO enrichment analysis for SGTC_1928
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2351.02E-74SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0338983TSC3-RPN4
0.2276.69E-70SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.147059TSC3-RPN4
0.2212.13E-66SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0757576TSC3-RPN4
0.2193.15E-65SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.157895TSC3-RPN4
0.2151.34E-62SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0615385TSC3-RPN4
0.2061.40E-57SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.340426TSC3-RPN4
0.2002.88E-54SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.6TSC3-RPN4
0.1957.66E-52SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.3125TSC3-RPN4
0.1951.44E-51SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.15TSC3-RPN4
0.1871.52E-47SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.291667
0.1871.37E-47SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.209677TSC3-RPN4
0.1771.17E-42SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0877193TSC3-RPN4
0.1761.73E-42SGTC_1891st059073 26.2 μMTimTec (Natural product derivative library)6887540.0740741TSC3-RPN4
0.1763.51E-42SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.101449
0.1743.05E-41SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.156863TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_24984'-Methoxychalcone54.63 μM1641818Microsource (Natural product library)238.281163.68502RPP1 & pyrimidine depletion
SGTC_26044'-hydroxychalcone11.32 μM0.6285715282362Microsource (Natural product library)224.254583.4612
SGTC_11730443-0020122 μM0.65331295ChemDiv (Drug-like library)254.280563.44313TSC3-RPN4
SGTC_1813st0512468.6 μM0.5641035291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4
SGTC_1608st00147340.5 μM0.5405417292267TimTec (Natural product derivative library)242.700244.36601
SGTC_1737st03555769.7 μM0.5116285716916TimTec (Natural product derivative library)286.75284.69802
SGTC_25772',4'-dihydroxy-4-methoxychalcone15.32 μM0.55711223Microsource (Natural product library)270.279963.20124
SGTC_25792',5'-dihydroxy-4-methoxychalcone13.23 μM0.55355888Microsource (Natural product library)270.279963.20124
SGTC_13041165-0502251 μM0.465116767425ChemDiv (Drug-like library)289.34952.24314
SGTC_15101123-020071.5 μM0.4634155378812ChemDiv (Drug-like library)313.392365.39602