genistein trimethyl ether

5,7-dimethoxy-3-(4-methoxyphenyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1929
Screen concentration 50.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 136420
SMILES COC1=CC=C(C=C1)C2=COC3=CC(=CC(=C3C2=O)OC)OC
Standardized SMILES COc1ccc(cc1)C2=COc3cc(OC)cc(OC)c3C2=O
Molecular weight 312.3166
ALogP 2.82
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.14
% growth inhibition (Hom. pool) 6.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 136420
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.88|P-value:5.20E-5|Clearance:0.48||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALG2(YGL065C)|FD-Score:4.29|P-value:9.01E-6|Clearance:0.48||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:DBP6(YNR038W)|FD-Score:4.67|P-value:1.48E-6|Clearance:0.48||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:FIP1(YJR093C)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.08||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:MAS1(YLR163C)|FD-Score:-3.24|P-value:6.02E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NAT2(YGR147C)|FD-Score:-5.88|P-value:2.05E-9|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOC2(YOR206W)|FD-Score:-4.12|P-value:1.93E-5|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NUF2(YOL069W)|FD-Score:-6.01|P-value:9.13E-10|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:PAP1(YKR002W)|FD-Score:4.41|P-value:5.10E-6|Clearance:0.48||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PZF1(YPR186C)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.05||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RHO1(YPR165W)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.48||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:SRM1(YGL097W)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.28||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:TAF6(YGL112C)|FD-Score:4.65|P-value:1.70E-6|Clearance:0.48||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TAM41(YGR046W)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TRS31(YDR472W)|FD-Score:-3.27|P-value:5.34E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UTP15(YMR093W)|FD-Score:-3.22|P-value:6.41E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:VTI1(YMR197C)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YJR141W(YJR141W_p)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.05||SGD DESC:Essential protein of unknown function Gene:ACT1(YFL039C)|FD-Score:3.88|P-value:5.20E-5|Clearance:0.48||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALG2(YGL065C)|FD-Score:4.29|P-value:9.01E-6|Clearance:0.48||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:DBP6(YNR038W)|FD-Score:4.67|P-value:1.48E-6|Clearance:0.48||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:FIP1(YJR093C)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.08||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:MAS1(YLR163C)|FD-Score:-3.24|P-value:6.02E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NAT2(YGR147C)|FD-Score:-5.88|P-value:2.05E-9|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NOC2(YOR206W)|FD-Score:-4.12|P-value:1.93E-5|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NUF2(YOL069W)|FD-Score:-6.01|P-value:9.13E-10|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:PAP1(YKR002W)|FD-Score:4.41|P-value:5.10E-6|Clearance:0.48||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PZF1(YPR186C)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.05||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RHO1(YPR165W)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.48||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:SRM1(YGL097W)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.28||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:TAF6(YGL112C)|FD-Score:4.65|P-value:1.70E-6|Clearance:0.48||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TAM41(YGR046W)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TRS31(YDR472W)|FD-Score:-3.27|P-value:5.34E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UTP15(YMR093W)|FD-Score:-3.22|P-value:6.41E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:VTI1(YMR197C)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YJR141W(YJR141W_p)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.05||SGD DESC:Essential protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 136420
Download HOP data (tab-delimited text)  (excel)
Gene:ADH5(YBR145W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AEP1(YMR064W)|FD-Score:-3.3|P-value:4.91E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AGA2(YGL032C)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIM14(YGL160W)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM34(YMR003W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:BCK1(YJL095W)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUD14(YAR014C)|FD-Score:-4.65|P-value:1.66E-6||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CCH1(YGR217W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CLN3(YAL040C)|FD-Score:6.37|P-value:9.25E-11||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CNE1(YAL058W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CPR4(YCR069W)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CSM1(YCR086W)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DDI3(YNL335W_p)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DXO1(YDR370C_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:ESBP6(YNL125C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:FMP37(YGL080W)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FRA1(YLL029W)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GDB1(YPR184W)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEX2(YKR106W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GSP2(YOR185C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HSV2(YGR223C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:IRS4(YKR019C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KDX1(YKL161C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:KIN2(YLR096W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LCL1(YPL056C_p)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LSC2(YGR244C)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAG1(YER142C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MCX1(YBR227C)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MNT4(YNR059W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:PAM1(YDR251W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PET112(YBL080C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PHO81(YGR233C)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PTC1(YDL006W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RBL2(YOR265W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:REC104(YHR157W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RHB1(YCR027C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RHO5(YNL180C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RHR2(YIL053W)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL38(YLR325C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPS22A(YJL190C)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:SBH1(YER087C-B)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEC28(YIL076W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SGF73(YGL066W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SHB17(YKR043C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SIL1(YOL031C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIT1(YEL065W)|FD-Score:5.21|P-value:9.46E-8||SGD DESC:Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p Gene:SKI2(YLR398C)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SPO12(YHR152W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SRP40(YKR092C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSH1(YBR283C)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SWC7(YLR385C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:TBS1(YBR150C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TMA7(YLR262C-A)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRX1(YLR043C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:VPS74(YDR372C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YFL012W(YFL012W_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGR079W(YGR079W_p)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YGR210C(YGR210C_p)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR049C-A(YHR049C-A_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL077W(YKL077W_p)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YMR034C(YMR034C_p)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOL164W-A(YOL164W-A_p)|FD-Score:5.33|P-value:4.96E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR139C(YOR139C_d)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL073C(YPL073C_d)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:ZDS1(YMR273C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication Gene:ADH5(YBR145W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AEP1(YMR064W)|FD-Score:-3.3|P-value:4.91E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AGA2(YGL032C)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIM14(YGL160W)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM34(YMR003W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:BCK1(YJL095W)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUD14(YAR014C)|FD-Score:-4.65|P-value:1.66E-6||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CCH1(YGR217W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CLN3(YAL040C)|FD-Score:6.37|P-value:9.25E-11||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CNE1(YAL058W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CPR4(YCR069W)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CSM1(YCR086W)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DDI3(YNL335W_p)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DXO1(YDR370C_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:ESBP6(YNL125C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:FMP37(YGL080W)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FRA1(YLL029W)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GDB1(YPR184W)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEX2(YKR106W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GSP2(YOR185C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HSV2(YGR223C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:IRS4(YKR019C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KDX1(YKL161C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:KIN2(YLR096W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LCL1(YPL056C_p)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LSC2(YGR244C)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAG1(YER142C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MCX1(YBR227C)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MNT4(YNR059W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:PAM1(YDR251W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PET112(YBL080C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PHO81(YGR233C)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PTC1(YDL006W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RBL2(YOR265W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:REC104(YHR157W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RHB1(YCR027C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RHO5(YNL180C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RHR2(YIL053W)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL38(YLR325C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPS22A(YJL190C)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:SBH1(YER087C-B)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEC28(YIL076W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SGF73(YGL066W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SHB17(YKR043C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SIL1(YOL031C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIT1(YEL065W)|FD-Score:5.21|P-value:9.46E-8||SGD DESC:Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p Gene:SKI2(YLR398C)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SPO12(YHR152W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SRP40(YKR092C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSH1(YBR283C)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SWC7(YLR385C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:TBS1(YBR150C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TMA7(YLR262C-A)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRX1(YLR043C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:VPS74(YDR372C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YFL012W(YFL012W_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGR079W(YGR079W_p)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YGR210C(YGR210C_p)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR049C-A(YHR049C-A_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL077W(YKL077W_p)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YMR034C(YMR034C_p)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOL164W-A(YOL164W-A_p)|FD-Score:5.33|P-value:4.96E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR139C(YOR139C_d)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL073C(YPL073C_d)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:ZDS1(YMR273C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YNR038W4.671.48E-60.48DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YGL112C4.651.70E-60.48TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YKR002W4.415.10E-60.48PAP1Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
YGL065C4.299.01E-60.48ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YPR165W3.973.59E-50.48RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YFL039C3.885.20E-50.48ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YJR141W_p3.403.33E-40.05YJR141W_pEssential protein of unknown function
YJR093C3.354.06E-40.08FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YPR186C3.275.32E-40.05PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YGL097W3.236.26E-40.28SRM1Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
YHR190W2.950.001580.22ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YKL006C-A2.730.003200.04SFT1Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment
YOR194C2.680.003650.01TOA1TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
YGL145W2.670.003780.04TIP20Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
YIL068C2.630.004240.11SEC6Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YAL040C6.379.25E-11CLN3G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis
YGR244C5.581.22E-8LSC2Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
YOL031C5.561.33E-8SIL1Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein
YOL164W-A_p5.334.96E-8YOL164W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YEL065W5.219.46E-8SIT1Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
YJL095W5.062.12E-7BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YGR233C4.836.77E-7PHO81Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress
YGL160W4.532.89E-6AIM14NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
YOL138C4.425.02E-6RTC1Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif
YAL058W4.141.70E-5CNE1Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
YKR019C4.092.14E-5IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YLR325C4.013.00E-5RPL38Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog
YOR185C3.973.54E-5GSP2GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress
YKL161C3.904.90E-5KDX1Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
YGR210C_p3.885.17E-5YGR210C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

GO enrichment analysis for SGTC_1929
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1411.69E-27SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0746269cell wall signaling
0.1125.50E-18SGTC_30929117248 49.5 μMChembridge (Drug-like library)171418570.108108cell wall signaling
0.1064.14E-16SGTC_19284'-methoxychalcone 40.3 μMTimTec (Natural product derivative library)6418180.222222
0.1001.22E-14SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.0677966cell wall signaling
0.0993.25E-14SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.0727273cell wall signaling
0.0952.99E-13SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.113636cell wall signaling
0.0921.65E-12SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.0493827cell wall signaling
0.0881.15E-11SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.148649cell wall
0.0855.14E-11SGTC_20315180455 200.0 μMChembridge (Fragment library)299490.0666667
0.0855.13E-11SGTC_483fpl-64176 144.0 μMICCB bioactive library34230.102941cell wall
0.0831.74E-10SGTC_20094012297 12.1 μMChembridge (Fragment library)455953070.078125cell wall signaling
0.0822.89E-10SGTC_5914299-0174 154.0 μMChemDiv (Drug-like library)55090880.164179
0.0791.28E-9SGTC_33319141060 2.0 μMChembridge (Drug-like library)49065670.0759494cell wall
0.0782.03E-9SGTC_422cantharidin 155.0 μMICCB bioactive library25450.0961538cell wall signaling
0.0765.39E-9SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.0967742cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2411st07713339.94 μM0.7173911403142TimTec (Natural product derivative library)368.336842.38507RNA pol III & RNase P/MRP
SGTC_2557derrustone83.84 μM0.717391629853Microsource (Natural product library)326.300162.60106
SGTC_2522biochanin a4.94 μM0.6444445280373Microsource (Natural product library)284.263482.36625RNA pol III & RNase P/MRP
SGTC_2620irigenin100 μM0.4615385464170Microsource (Natural product library)360.314842.09138
SGTC_2550chrysin dimethyl ether71.87 μM0.43137388881Microsource (Natural product library)282.290663.10304
SGTC_1548st06146890.8 μM0.425532390799TimTec (Pure natural product library)220.221282.31304
SGTC_25903,4',5',6',7'-pentamethoxyflavone100 μM0.415094521171Microsource (Natural product library)372.36862.7580760S ribosome export
SGTC_26335,7-dihydroxyisoflavone100 μM0.4081635377381Microsource (Natural product library)254.23752.38224
SGTC_2636apigenin dimethyl ether100 μM0.4038465281601Microsource (Natural product library)298.290062.86115
SGTC_2492derrusnin100 μM0.4035091715307Microsource (Natural product library)356.326142.80107