st074717

4-[(4-cyclohexylpiperazin-1-yl)methyl]-5,7-dimethylchromen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1938
Screen concentration 56.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 17250979
SMILES CC1=CC(=C2C(=CC(=O)OC2=C1)CN3CCN(CC3)C4CCCCC4)C
Standardized SMILES Cc1cc(C)c2C(=CC(=O)Oc2c1)CN3CCN(CC3)C4CCCCC4
Molecular weight 354.4858
ALogP 4.72
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.3
% growth inhibition (Hom. pool) -1.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17250979
Download HIP data (tab-delimited text)  (excel)
Gene:BDP1(YNL039W)|FD-Score:-3.37|P-value:3.76E-4|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:BMS1(YPL217C)|FD-Score:-3.9|P-value:4.74E-5|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDC1(YDR182W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.31||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:CDC24(YAL041W)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAS2(YHR024C)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MVD1(YNR043W)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.31||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NAB3(YPL190C)|FD-Score:-3.48|P-value:2.47E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NHP2(YDL208W)|FD-Score:-3.21|P-value:6.65E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:NUD1(YOR373W)|FD-Score:-4.86|P-value:6.02E-7|Clearance:0||SGD DESC:Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance Gene:RFA1(YAR007C)|FD-Score:-4.21|P-value:1.25E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:SEC20(YDR498C)|FD-Score:3.19|P-value:7.03E-4|Clearance:0.31||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SEC23(YPR181C)|FD-Score:-5.1|P-value:1.67E-7|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TIP20(YGL145W)|FD-Score:-4.16|P-value:1.60E-5|Clearance:0||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p Gene:YPD1(YDL235C)|FD-Score:-3.61|P-value:1.51E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YPR177C(YPR177C_d)|FD-Score:-3.38|P-value:3.57E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:BDP1(YNL039W)|FD-Score:-3.37|P-value:3.76E-4|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:BMS1(YPL217C)|FD-Score:-3.9|P-value:4.74E-5|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDC1(YDR182W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.31||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:CDC24(YAL041W)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAS2(YHR024C)|FD-Score:-3.32|P-value:4.54E-4|Clearance:0||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MVD1(YNR043W)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.31||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NAB3(YPL190C)|FD-Score:-3.48|P-value:2.47E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NHP2(YDL208W)|FD-Score:-3.21|P-value:6.65E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:NUD1(YOR373W)|FD-Score:-4.86|P-value:6.02E-7|Clearance:0||SGD DESC:Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance Gene:RFA1(YAR007C)|FD-Score:-4.21|P-value:1.25E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:SEC20(YDR498C)|FD-Score:3.19|P-value:7.03E-4|Clearance:0.31||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SEC23(YPR181C)|FD-Score:-5.1|P-value:1.67E-7|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TIP20(YGL145W)|FD-Score:-4.16|P-value:1.60E-5|Clearance:0||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p Gene:YPD1(YDL235C)|FD-Score:-3.61|P-value:1.51E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YPR177C(YPR177C_d)|FD-Score:-3.38|P-value:3.57E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17250979
Download HOP data (tab-delimited text)  (excel)
Gene:ATE1(YGL017W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:ATG8(YBL078C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:CAP2(YIL034C)|FD-Score:6.47|P-value:4.95E-11||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CLD1(YGR110W)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CMC4(YMR194C-B)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CNB1(YKL190W)|FD-Score:6.3|P-value:1.47E-10||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COX7(YMR256C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CPR2(YHR057C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CRP1(YHR146W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Protein that binds to cruciform DNA structures Gene:CYB2(YML054C)|FD-Score:-3.76|P-value:8.60E-5||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:EDS1(YBR033W_p)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EIS1(YMR031C)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:FMP52(YER004W)|FD-Score:-3.79|P-value:7.63E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:HBN1(YCL026C-B_p)|FD-Score:3.77|P-value:8.27E-5||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HHF1(YBR009C)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HSE1(YHL002W)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:LAS21(YJL062W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MCH5(YOR306C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MGR2(YPL098C)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MIP1(YOR330C)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MND2(YIR025W)|FD-Score:5.9|P-value:1.87E-9||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNL2(YLR057W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MON2(YNL297C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MOT2(YER068W)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:PCI8(YIL071C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PIP2(YOR363C)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:RCR1(YBR005W)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:REX4(YOL080C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly Gene:RNR4(YGR180C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL6A(YML073C)|FD-Score:5.99|P-value:1.02E-9||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RSC2(YLR357W)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:SAN1(YDR143C)|FD-Score:-3.8|P-value:7.21E-5||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SHE3(YBR130C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SLG1(YOR008C)|FD-Score:-4.83|P-value:6.75E-7||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNF6(YHL025W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNT2(YGL131C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SRO7(YPR032W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:-4.58|P-value:2.33E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM70(YNL121C)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM732(YMR259C_p)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRM82(YDR165W)|FD-Score:-5.04|P-value:2.31E-7||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBP5(YER144C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VMA5(YKL080W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH1(YOR270C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS27(YNR006W)|FD-Score:4.49|P-value:3.62E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS45(YGL095C)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS51(YKR020W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:-5.91|P-value:1.68E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAP5(YIR018W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL049C(YCL049C_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR016W(YCR016W_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR099C(YCR099C_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function Gene:YER087C-A(YER087C-A_d)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YGR107W(YGR107W_d)|FD-Score:-3.99|P-value:3.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR204C-A(YGR204C-A_p)|FD-Score:4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YHR182W(YHR182W_p)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YJL175W(YJL175W_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YLR173W(YLR173W_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function Gene:YMR193C-A(YMR193C-A_d)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR209C(YMR209C_p)|FD-Score:-3.81|P-value:7.01E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR265C(YMR265C_p)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Putative protein of unknown function Gene:YNL050C(YNL050C_p)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:-4.27|P-value:9.89E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ATE1(YGL017W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:ATG8(YBL078C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:CAP2(YIL034C)|FD-Score:6.47|P-value:4.95E-11||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CLD1(YGR110W)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CMC4(YMR194C-B)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CNB1(YKL190W)|FD-Score:6.3|P-value:1.47E-10||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COX7(YMR256C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CPR2(YHR057C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CRP1(YHR146W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Protein that binds to cruciform DNA structures Gene:CYB2(YML054C)|FD-Score:-3.76|P-value:8.60E-5||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:EDS1(YBR033W_p)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EIS1(YMR031C)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:FMP52(YER004W)|FD-Score:-3.79|P-value:7.63E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:HBN1(YCL026C-B_p)|FD-Score:3.77|P-value:8.27E-5||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HHF1(YBR009C)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HSE1(YHL002W)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:LAS21(YJL062W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MCH5(YOR306C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MGR2(YPL098C)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MIP1(YOR330C)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MND2(YIR025W)|FD-Score:5.9|P-value:1.87E-9||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNL2(YLR057W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MON2(YNL297C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MOT2(YER068W)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:PCI8(YIL071C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PIP2(YOR363C)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:RCR1(YBR005W)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:REX4(YOL080C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly Gene:RNR4(YGR180C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL6A(YML073C)|FD-Score:5.99|P-value:1.02E-9||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RSC2(YLR357W)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:SAN1(YDR143C)|FD-Score:-3.8|P-value:7.21E-5||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SHE3(YBR130C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SLG1(YOR008C)|FD-Score:-4.83|P-value:6.75E-7||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNF6(YHL025W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNT2(YGL131C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SRO7(YPR032W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:-4.58|P-value:2.33E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM70(YNL121C)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM732(YMR259C_p)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRM82(YDR165W)|FD-Score:-5.04|P-value:2.31E-7||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBP5(YER144C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VMA5(YKL080W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH1(YOR270C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS27(YNR006W)|FD-Score:4.49|P-value:3.62E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS45(YGL095C)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS51(YKR020W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:-5.91|P-value:1.68E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAP5(YIR018W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL049C(YCL049C_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR016W(YCR016W_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR099C(YCR099C_p)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function Gene:YER087C-A(YER087C-A_d)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YGR107W(YGR107W_d)|FD-Score:-3.99|P-value:3.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR204C-A(YGR204C-A_p)|FD-Score:4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YHR182W(YHR182W_p)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YJL175W(YJL175W_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YLR173W(YLR173W_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function Gene:YMR193C-A(YMR193C-A_d)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR209C(YMR209C_p)|FD-Score:-3.81|P-value:7.01E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR265C(YMR265C_p)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Putative protein of unknown function Gene:YNL050C(YNL050C_p)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:-4.27|P-value:9.89E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR182W3.691.10E-40.31CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
YNR043W3.492.43E-40.31MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YDR498C3.197.03E-40.31SEC20Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p
YDR082W2.880.001970.11STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YKL108W2.770.002810.03SLD2Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
YDR396W_d2.740.003060.15YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YIL091C2.590.004810.07UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YLR033W2.520.005891.98E-4RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YLL031C2.520.005890.15GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
YDR167W2.370.008910.02TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YKR079C2.350.009350.00TRZ1tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2
YOL034W2.350.009380.01SMC5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
YPL094C2.340.009590.02SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YLR243W_p2.320.010100.05GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YJR002W2.270.011500.01MPP10Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL034C6.474.95E-11CAP2Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
YKL190W6.301.47E-10CNB1Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress
YML073C5.991.02E-9RPL6ARibosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
YIR025W5.901.87E-9MND2Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
YER087C-A_d5.062.11E-7YER087C-A_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase
YNR006W4.493.62E-6VPS27Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
YPR032W4.395.70E-6SRO7Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
YOR306C4.171.54E-5MCH5Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport
YMR256C4.042.68E-5COX7Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YGR204C-A_p4.003.23E-5YGR204C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YLR357W3.983.47E-5RSC2Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance
YHR146W3.973.64E-5CRP1Protein that binds to cruciform DNA structures
YHR057C3.807.30E-5CPR2Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway
YOR330C3.797.67E-5MIP1Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
YCL026C-B_p3.778.27E-5HBN1_pProtein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1938
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1674.76E-38SGTC_1946st076057 65.1 μMTimTec (Natural product derivative library)9530090.144737
0.1048.80E-16SGTC_1924st058459 75.1 μMTimTec (Natural product derivative library)6886830.138889
0.0732.06E-8SGTC_2763lisinopril 49.3 μMMiscellaneous53621190.11236
0.0699.87E-8SGTC_1948st076329 66.6 μMTimTec (Natural product derivative library)242084130.123457
0.0682.01E-7SGTC_13211326-2144 215.0 μMChemDiv (Drug-like library)35130300.0675676
0.0672.22E-7SGTC_12201838-0075 193.0 μMChemDiv (Drug-like library)55296450.0588235
0.0663.69E-7SGTC_33139133968 57.0 μMChembridge (Drug-like library)14946430.127907
0.0647.75E-7SGTC_2407paclitaxel 400.0 μMMiscellaneous46660.0725806
0.0631.21E-6SGTC_12660828-0227 32.9 μMChemDiv (Drug-like library)39138200.115942
0.0621.62E-6SGTC_11921488-0095 21.4 μMChemDiv (Drug-like library)9158750.0779221
0.0612.44E-6SGTC_12001643-0193 18.4 μMChemDiv (Drug-like library)54039740.0888889
0.0603.49E-6SGTC_30849116877 49.5 μMChembridge (Drug-like library)272413250.0930233
0.0604.14E-6SGTC_2643anethole 100.0 μMMicrosource (Natural product library)6375630.047619
0.0596.48E-6SGTC_3174495-0002 170.0 μMChemDiv (Drug-like library)159916180.0487805
0.0588.68E-6SGTC_7192810-4230 27.3 μMChemDiv (Drug-like library)7210790.0526316

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1999st07483112.5 μM0.42857117112523TimTec (Natural product derivative library)382.883224.62504plasma membrane duress
SGTC_1937st07471447.2 μM0.37837817250970TimTec (Natural product derivative library)423.4799433.70516
SGTC_6464092-083927.3 μM0.375877796ChemDiv (Drug-like library)306.873384.83902plasma membrane duress
SGTC_1933st07471115.9 μM0.36486517250958TimTec (Natural product derivative library)463.955944.80706
SGTC_1934st07472032.6 μM0.30487817250992TimTec (Natural product derivative library)493.981924.65407
SGTC_2014262-015912.8 μM0.31341029ChemDiv (Drug-like library)300.481484.91302ergosterol depletion effects on membrane
SGTC_1932st07480640.9 μM0.29761917251235TimTec (Natural product derivative library)489.562843.97308
SGTC_1902st06021270.5 μM0.274194688944TimTec (Natural product derivative library)204.221882.42203
SGTC_10054092-06904.4 μM0.2647061529343ChemDiv (Drug-like library)364.523684.98603plasma membrane duress
SGTC_3204092-08465.34 μM0.2638891359356ChemDiv (Drug-like library)394.549664.9704ergosterol depletion effects on membrane