st076331

8-[(2-chlorophenyl)methylsulfanyl]-9-methyl-3H-purin-6-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1943
Screen concentration 65.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 24208415
SMILES CN1C2=C(C(=O)N=CN2)N=C1SCC3=CC=CC=C3Cl
Standardized SMILES Cn1c(SCc2ccccc2Cl)nc3c(O)ncnc13
Molecular weight 306.7706
ALogP 2.73
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.81
% growth inhibition (Hom. pool) -5.45


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24208415
Download HIP data (tab-delimited text)  (excel)
Gene:CDC15(YAR019C)|FD-Score:-3.1|P-value:9.72E-4|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:ERG12(YMR208W)|FD-Score:3.2|P-value:6.94E-4|Clearance:0.07||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:PMI40(YER003C)|FD-Score:4.33|P-value:7.33E-6|Clearance:0.76||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RPT3(YDR394W)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.21||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.11||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RRS1(YOR294W)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.13||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SLY1(YDR189W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.08||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:TAF6(YGL112C)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.06||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:YJR141W(YJR141W_p)|FD-Score:-3.15|P-value:8.21E-4|Clearance:0||SGD DESC:Essential protein of unknown function Gene:CDC15(YAR019C)|FD-Score:-3.1|P-value:9.72E-4|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:ERG12(YMR208W)|FD-Score:3.2|P-value:6.94E-4|Clearance:0.07||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:PMI40(YER003C)|FD-Score:4.33|P-value:7.33E-6|Clearance:0.76||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RPT3(YDR394W)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.21||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.11||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RRS1(YOR294W)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.13||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SLY1(YDR189W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.08||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:TAF6(YGL112C)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.06||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:YJR141W(YJR141W_p)|FD-Score:-3.15|P-value:8.21E-4|Clearance:0||SGD DESC:Essential protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24208415
Download HOP data (tab-delimited text)  (excel)
Gene:ACS1(YAL054C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:APJ1(YNL077W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARL1(YBR164C)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO7(YPR060C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARR1(YPR199C)|FD-Score:-3.97|P-value:3.61E-5||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ASR1(YPR093C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:BFR1(YOR198C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BST1(YFL025C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD14(YAR014C)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CIN4(YMR138W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COG6(YNL041C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DLS1(YJL065C)|FD-Score:3.77|P-value:8.19E-5||SGD DESC:Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication Gene:DTD1(YDL219W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:EAR1(YMR171C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:ECM22(YLR228C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ECM32(YER176W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:EMC4(YGL231C)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:FOB1(YDR110W)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FTH1(YBR207W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress Gene:FUI1(YBL042C)|FD-Score:4.76|P-value:9.57E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:HOM2(YDR158W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:INP1(YMR204C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:MAE1(YKL029C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MED1(YPR070W)|FD-Score:4.81|P-value:7.71E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET14(YKL001C)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MNN2(YBR015C)|FD-Score:5.28|P-value:6.32E-8||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOS1(YCL057C-A)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MSS11(YMR164C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:MUB1(YMR100W)|FD-Score:-6.28|P-value:1.66E-10||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:MXR2(YCL033C)|FD-Score:-4.28|P-value:9.43E-6||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NDE2(YDL085W)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:PCI8(YIL071C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEP7(YDR323C)|FD-Score:5.27|P-value:6.94E-8||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET54(YGR222W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PRR1(YKL116C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:QCR6(YFR033C)|FD-Score:7.75|P-value:4.53E-15||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RAD54(YGL163C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RNP1(YLL046C)|FD-Score:-4.49|P-value:3.48E-6||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL37A(YLR185W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication Gene:RPL41B(YDL133C-A)|FD-Score:-3.81|P-value:6.88E-5||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SNC2(YOR327C)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SPA2(YLL021W)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SYH1(YPL105C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TOM70(YNL121C)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TOR1(YJR066W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TPO5(YKL174C)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:UIP4(YPL186C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VEL1(YGL258W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS29(YHR012W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS5(YOR069W)|FD-Score:-3.9|P-value:4.90E-5||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR196C-B(YBR196C-B_p)|FD-Score:4.47|P-value:3.83E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YCL021W-A(YCL021W-A_p)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Putative protein of unknown function Gene:YCL049C(YCL049C_p)|FD-Score:-5.24|P-value:8.15E-8||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDL041W(YDL041W_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YGL088W(YGL088W_d)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGL114W(YGL114W_p)|FD-Score:-4.46|P-value:4.13E-6||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YGR107W(YGR107W_d)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR226C(YGR226C_d)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YGR228W(YGR228W_d)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHP1(YDR451C)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR202W(YHR202W_p)|FD-Score:5.1|P-value:1.68E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIL012W(YIL012W_d)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL135W(YJL135W_d)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YKR047W(YKR047W_d)|FD-Score:-5.24|P-value:7.87E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR173W(YLR173W_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function Gene:YPR027C(YPR027C_p)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Putative protein of unknown function Gene:YTA7(YGR270W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ZEO1(YOL109W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:ACS1(YAL054C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:APJ1(YNL077W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARL1(YBR164C)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO7(YPR060C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARR1(YPR199C)|FD-Score:-3.97|P-value:3.61E-5||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ASR1(YPR093C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:BFR1(YOR198C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BST1(YFL025C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD14(YAR014C)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CIN4(YMR138W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COG6(YNL041C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DLS1(YJL065C)|FD-Score:3.77|P-value:8.19E-5||SGD DESC:Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication Gene:DTD1(YDL219W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:EAR1(YMR171C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:ECM22(YLR228C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ECM32(YER176W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:EMC4(YGL231C)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:FOB1(YDR110W)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FTH1(YBR207W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress Gene:FUI1(YBL042C)|FD-Score:4.76|P-value:9.57E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:HOM2(YDR158W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:INP1(YMR204C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:MAE1(YKL029C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MED1(YPR070W)|FD-Score:4.81|P-value:7.71E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET14(YKL001C)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MNN2(YBR015C)|FD-Score:5.28|P-value:6.32E-8||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOS1(YCL057C-A)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MSS11(YMR164C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:MUB1(YMR100W)|FD-Score:-6.28|P-value:1.66E-10||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:MXR2(YCL033C)|FD-Score:-4.28|P-value:9.43E-6||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NDE2(YDL085W)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:PCI8(YIL071C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEP7(YDR323C)|FD-Score:5.27|P-value:6.94E-8||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET54(YGR222W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PRR1(YKL116C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:QCR6(YFR033C)|FD-Score:7.75|P-value:4.53E-15||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RAD54(YGL163C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RNP1(YLL046C)|FD-Score:-4.49|P-value:3.48E-6||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL37A(YLR185W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication Gene:RPL41B(YDL133C-A)|FD-Score:-3.81|P-value:6.88E-5||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SNC2(YOR327C)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SPA2(YLL021W)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SYH1(YPL105C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TOM70(YNL121C)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TOR1(YJR066W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TPO5(YKL174C)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:UIP4(YPL186C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VEL1(YGL258W)|FD-Score:4.39|P-value:5.70E-6||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS29(YHR012W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS5(YOR069W)|FD-Score:-3.9|P-value:4.90E-5||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR196C-B(YBR196C-B_p)|FD-Score:4.47|P-value:3.83E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YCL021W-A(YCL021W-A_p)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Putative protein of unknown function Gene:YCL049C(YCL049C_p)|FD-Score:-5.24|P-value:8.15E-8||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDL041W(YDL041W_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YGL088W(YGL088W_d)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGL114W(YGL114W_p)|FD-Score:-4.46|P-value:4.13E-6||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YGR107W(YGR107W_d)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR226C(YGR226C_d)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YGR228W(YGR228W_d)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHP1(YDR451C)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR202W(YHR202W_p)|FD-Score:5.1|P-value:1.68E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIL012W(YIL012W_d)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL135W(YJL135W_d)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YKR047W(YKR047W_d)|FD-Score:-5.24|P-value:7.87E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR173W(YLR173W_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function Gene:YPR027C(YPR027C_p)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Putative protein of unknown function Gene:YTA7(YGR270W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ZEO1(YOL109W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER003C4.337.33E-60.76PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YGL112C3.571.76E-40.06TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YOR294W3.512.20E-40.13RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YDR189W3.393.55E-40.08SLY1Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
YOR117W3.314.74E-40.11RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YMR208W3.206.94E-40.07ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YDR394W3.128.90E-40.21RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YDL058W2.910.001800.09USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YOR095C2.820.002400.14RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YGL099W2.690.003630.02LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YLR009W2.660.003880.10RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YBR152W2.560.005230.00SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YGL225W2.560.005260.02VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YMR203W2.540.005600.06TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YNL039W2.480.006580.02BDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFR033C7.754.53E-15QCR6Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
YBR015C5.286.32E-8MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YDR323C5.276.94E-8PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YGL231C5.121.51E-7EMC4Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85
YHR202W_p5.101.68E-7YHR202W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
YPR070W4.817.71E-7MED1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YBL042C4.769.57E-7FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YDR158W4.741.08E-6HOM2Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YPR027C_p4.711.24E-6YPR027C_pPutative protein of unknown function
YBR196C-B_p4.473.83E-6YBR196C-B_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YLR185W4.444.51E-6RPL37ARibosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication
YGL258W4.395.70E-6VEL1Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C
YGR222W4.102.07E-5PET54Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing
YOR014W3.865.71E-5RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YGR228W_d3.846.16E-5YGR228W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C

GO enrichment analysis for SGTC_1943
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2342.25E-74SGTC_1947st076332 61.6 μMTimTec (Natural product derivative library)242084160.703704
0.0849.00E-11SGTC_1107amlodipine 47.0 μMNIH Clinical Collection21620.146067
0.0822.79E-10SGTC_21785812956 200.0 μMChembridge (Fragment library)54033830.130435
0.0823.03E-10SGTC_469w7 172.0 μMICCB bioactive library451155700.166667ERG2
0.0808.91E-10SGTC_1465k213-0056 144.0 μMChemDiv (Drug-like library)240488570.173333DNA intercalators
0.0731.83E-8SGTC_7504073-0104 132.0 μMChemDiv (Drug-like library)223309570.164557
0.0717.48E-8SGTC_1460k062-0059 9.2 μMChemDiv (Drug-like library)13730850.0921053Golgi
0.0706.48E-8SGTC_412gemfibrozil 100.0 μMMiscellaneous34630.0810811DNA intercalators
0.0691.31E-7SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.129032DNA intercalators
0.0681.49E-7SGTC_33259138760 55.2 μMChembridge (Drug-like library)438449590.119048Golgi
0.0681.49E-7SGTC_33109130229 44.1 μMChembridge (Drug-like library)77612580.121622Golgi
0.0672.17E-7SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.111111DNA intercalators
0.0663.90E-7SGTC_1250133-0007 11.8 μMChemDiv (Drug-like library)265410.0892857DNA intercalators
0.0664.12E-7SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0340909DNA intercalators
0.0649.17E-7SGTC_28959054447 58.4 μMChembridge (Drug-like library)26974500.157143

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1947st07633261.6 μM0.70370424208416TimTec (Natural product derivative library)324.7611032.93314
SGTC_1942st07633065.2 μM0.68627524208414TimTec (Natural product derivative library)306.770642.72813
SGTC_1948st07632966.6 μM0.64814824208413TimTec (Natural product derivative library)300.378743.03613
SGTC_2707st07855666.6 μM0.47457617250899TimTec (Natural product derivative library)300.378742.89813
SGTC_1944st07637156.9 μM0.46969724208455TimTec (Natural product derivative library)351.3312461.52726
SGTC_2710st07856253.72 μM0.44776117250908TimTec (Natural product derivative library)357.430062.40724
SGTC_2708st07855956 μM0.38028217250902TimTec (Natural product derivative library)357.430062.43724
SGTC_2709st07856056 μM0.36111117250903TimTec (Natural product derivative library)357.430062.43724
SGTC_2692st07742647.7 μM0.35135125238571TimTec (Natural product derivative library)419.499444.12124
SGTC_1997st07302493.8 μM0.315789686268TimTec (Natural product derivative library)213.195480.58713