st075991

3-cyclohexyl-9,10-dimethoxy-2,4,6,7-tetrahydropyrimido[6,1-a]isoquinoline-1-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1945
Screen concentration 56.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 1084980
SMILES COC1=C(C=C2C(=C1)CCN3C2=C(CN(C3)C4CCCCC4)C#N)OC
Standardized SMILES COc1cc2CCN3CN(CC(=C3c2cc1OC)C#N)C4CCCCC4
Molecular weight 353.458
ALogP 3.68
H-bond donor count 0
H-bond acceptor count 5
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.97
% growth inhibition (Hom. pool) -4.16


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1084980
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC20(YGL116W)|FD-Score:-3.39|P-value:3.55E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CEG1(YGL130W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.11||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:COP1(YDL145C)|FD-Score:4.63|P-value:1.80E-6|Clearance:0.03||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:CSE1(YGL238W)|FD-Score:-4.12|P-value:1.90E-5|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:DAD1(YDR016C)|FD-Score:3.34|P-value:4.21E-4|Clearance:0.04||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DRS1(YLL008W)|FD-Score:-3.6|P-value:1.56E-4|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:KRE5(YOR336W)|FD-Score:3.9|P-value:4.73E-5|Clearance:0.01||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:LSG1(YGL099W)|FD-Score:5.59|P-value:1.11E-8|Clearance:0.72||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MGE1(YOR232W)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.34||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:MTR3(YGR158C)|FD-Score:3.15|P-value:8.27E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NMD3(YHR170W)|FD-Score:4.87|P-value:5.46E-7|Clearance:0.24||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP2(YNL061W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.01||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RER2(YBR002C)|FD-Score:3.8|P-value:7.32E-5|Clearance:0.08||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLP24(YLR009W)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.02||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPA43(YOR340C)|FD-Score:3.84|P-value:6.13E-5|Clearance:0.04||SGD DESC:RNA polymerase I subunit A43 Gene:RPC19(YNL113W)|FD-Score:3.89|P-value:4.96E-5|Clearance:0.03||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPO26(YPR187W)|FD-Score:4.6|P-value:2.06E-6|Clearance:0.36||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRB1(YMR131C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.03||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:STH1(YIL126W)|FD-Score:5.66|P-value:7.75E-9|Clearance:0.72||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TFB1(YDR311W)|FD-Score:-3.19|P-value:7.22E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TOM40(YMR203W)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.15||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRS120(YDR407C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.08||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YDL152W(YDL152W_d)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YLR198C(YLR198C_d)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:ARP9(YMR033W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC20(YGL116W)|FD-Score:-3.39|P-value:3.55E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CEG1(YGL130W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.11||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:COP1(YDL145C)|FD-Score:4.63|P-value:1.80E-6|Clearance:0.03||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:CSE1(YGL238W)|FD-Score:-4.12|P-value:1.90E-5|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:DAD1(YDR016C)|FD-Score:3.34|P-value:4.21E-4|Clearance:0.04||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DRS1(YLL008W)|FD-Score:-3.6|P-value:1.56E-4|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:KRE5(YOR336W)|FD-Score:3.9|P-value:4.73E-5|Clearance:0.01||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:LSG1(YGL099W)|FD-Score:5.59|P-value:1.11E-8|Clearance:0.72||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MGE1(YOR232W)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.34||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:MTR3(YGR158C)|FD-Score:3.15|P-value:8.27E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NMD3(YHR170W)|FD-Score:4.87|P-value:5.46E-7|Clearance:0.24||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP2(YNL061W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.01||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RER2(YBR002C)|FD-Score:3.8|P-value:7.32E-5|Clearance:0.08||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLP24(YLR009W)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.02||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPA43(YOR340C)|FD-Score:3.84|P-value:6.13E-5|Clearance:0.04||SGD DESC:RNA polymerase I subunit A43 Gene:RPC19(YNL113W)|FD-Score:3.89|P-value:4.96E-5|Clearance:0.03||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPO26(YPR187W)|FD-Score:4.6|P-value:2.06E-6|Clearance:0.36||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRB1(YMR131C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.03||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:STH1(YIL126W)|FD-Score:5.66|P-value:7.75E-9|Clearance:0.72||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TFB1(YDR311W)|FD-Score:-3.19|P-value:7.22E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TOM40(YMR203W)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.15||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRS120(YDR407C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.08||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YDL152W(YDL152W_d)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YLR198C(YLR198C_d)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1084980
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADY2(YCR010C)|FD-Score:-8.53|P-value:7.42E-18||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AHC2(YCR082W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALP1(YNL270C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:ARR1(YPR199C)|FD-Score:-4.44|P-value:4.54E-6||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:BNA3(YJL060W)|FD-Score:4.35|P-value:6.80E-6||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BUD16(YEL029C)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CST9(YLR394W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:CTI6(YPL181W)|FD-Score:-4.07|P-value:2.39E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CYC1(YJR048W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:ELP4(YPL101W)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERV14(YGL054C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FCY2(YER056C)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV5(YCL058C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GET2(YER083C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GIC2(YDR309C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region Gene:GYP1(YOR070C)|FD-Score:5.92|P-value:1.62E-9||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:IKI1(YHR187W)|FD-Score:5.32|P-value:5.09E-8||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IRC11(YOR013W_d)|FD-Score:5.42|P-value:2.99E-8||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:ISU1(YPL135W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:JSN1(YJR091C)|FD-Score:-3.13|P-value:8.59E-4||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:KGD2(YDR148C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:LDB17(YDL146W)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LEE1(YPL054W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Zinc-finger protein of unknown function Gene:MAC1(YMR021C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MNL2(YLR057W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRPL13(YKR006C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL6(YHR147C)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB3(YNL293W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSC2(YDR205W)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSC7(YHR039C)|FD-Score:4.32|P-value:7.63E-6||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NUP2(YLR335W)|FD-Score:-5.1|P-value:1.71E-7||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OSH6(YKR003W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PCL9(YDL179W)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PEX30(YLR324W)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PST1(YDR055W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:RAD5(YLR032W)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD6(YGL058W)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RKM2(YDR198C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:RPH1(YER169W)|FD-Score:-3.73|P-value:9.73E-5||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:3.73|P-value:9.40E-5||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:-3.13|P-value:8.73E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SDC25(YLL016W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SDP1(YIL113W)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SFH5(YJL145W)|FD-Score:4.88|P-value:5.22E-7||SGD DESC:Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Gene:SLX1(YBR228W)|FD-Score:-5.45|P-value:2.54E-8||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SOL1(YNR034W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPI1(YER150W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SRC1(YML034W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SRO77(YBL106C)|FD-Score:-4.14|P-value:1.72E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:THR1(YHR025W)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TPN1(YGL186C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBX2(YML013W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UGA2(YBR006W)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:USA1(YML029W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VAC8(YEL013W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VMA4(YOR332W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS3(YDR495C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBL095W(YBL095W_p)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR051W(YBR051W_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR296C-A(YBR296C-A_p)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDL068W(YDL068W_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR306C(YDR306C_p)|FD-Score:-4.87|P-value:5.62E-7||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YEL1(YBL060W)|FD-Score:4.79|P-value:8.24E-7||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YFR035C(YFR035C_p)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGR064W(YGR064W_d)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHR177W(YHR177W_p)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YJL064W(YJL064W_d)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YKL027W(YKL027W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKR104W(YKR104W)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR152C(YLR152C_p)|FD-Score:-4.81|P-value:7.42E-7||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YMR122C(YMR122C_d)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR141C(YMR141C_d)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR265C(YMR265C_p)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Putative protein of unknown function Gene:YMR316C-A(YMR316C-A_p)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YMR320W(YMR320W_d)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL043C(YNL043C_d)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YOL035C(YOL035C_d)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR333C(YOR333C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth Gene:ZRC1(YMR243C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADY2(YCR010C)|FD-Score:-8.53|P-value:7.42E-18||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AHC2(YCR082W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALP1(YNL270C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:ARR1(YPR199C)|FD-Score:-4.44|P-value:4.54E-6||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:BNA3(YJL060W)|FD-Score:4.35|P-value:6.80E-6||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BUD16(YEL029C)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CST9(YLR394W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:CTI6(YPL181W)|FD-Score:-4.07|P-value:2.39E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CYC1(YJR048W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:ELP4(YPL101W)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERV14(YGL054C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FCY2(YER056C)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV5(YCL058C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GET2(YER083C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GIC2(YDR309C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region Gene:GYP1(YOR070C)|FD-Score:5.92|P-value:1.62E-9||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:IKI1(YHR187W)|FD-Score:5.32|P-value:5.09E-8||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IRC11(YOR013W_d)|FD-Score:5.42|P-value:2.99E-8||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:ISU1(YPL135W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:JSN1(YJR091C)|FD-Score:-3.13|P-value:8.59E-4||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:KGD2(YDR148C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:LDB17(YDL146W)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LEE1(YPL054W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Zinc-finger protein of unknown function Gene:MAC1(YMR021C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MNL2(YLR057W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRPL13(YKR006C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL6(YHR147C)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB3(YNL293W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSC2(YDR205W)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSC7(YHR039C)|FD-Score:4.32|P-value:7.63E-6||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NUP2(YLR335W)|FD-Score:-5.1|P-value:1.71E-7||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OSH6(YKR003W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PCL9(YDL179W)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PEX30(YLR324W)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PST1(YDR055W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:RAD5(YLR032W)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD6(YGL058W)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RKM2(YDR198C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:RPH1(YER169W)|FD-Score:-3.73|P-value:9.73E-5||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:3.73|P-value:9.40E-5||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:-3.13|P-value:8.73E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SDC25(YLL016W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SDP1(YIL113W)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SFH5(YJL145W)|FD-Score:4.88|P-value:5.22E-7||SGD DESC:Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Gene:SLX1(YBR228W)|FD-Score:-5.45|P-value:2.54E-8||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SOL1(YNR034W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPI1(YER150W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SRC1(YML034W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SRO77(YBL106C)|FD-Score:-4.14|P-value:1.72E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:THR1(YHR025W)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TPN1(YGL186C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBX2(YML013W)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UGA2(YBR006W)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:USA1(YML029W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VAC8(YEL013W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VMA4(YOR332W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS3(YDR495C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YBL095W(YBL095W_p)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR051W(YBR051W_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR296C-A(YBR296C-A_p)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDL068W(YDL068W_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR306C(YDR306C_p)|FD-Score:-4.87|P-value:5.62E-7||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YEL1(YBL060W)|FD-Score:4.79|P-value:8.24E-7||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YFR035C(YFR035C_p)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGR064W(YGR064W_d)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHR177W(YHR177W_p)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YJL064W(YJL064W_d)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YKL027W(YKL027W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKR104W(YKR104W)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR152C(YLR152C_p)|FD-Score:-4.81|P-value:7.42E-7||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YMR122C(YMR122C_d)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR141C(YMR141C_d)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR265C(YMR265C_p)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Putative protein of unknown function Gene:YMR316C-A(YMR316C-A_p)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YMR320W(YMR320W_d)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL043C(YNL043C_d)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YOL035C(YOL035C_d)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR333C(YOR333C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth Gene:ZRC1(YMR243C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL126W5.667.75E-90.72STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YGL099W5.591.11E-80.72LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W4.875.46E-70.24NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDL145C4.631.80E-60.03COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YPR187W4.602.06E-60.36RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YOR232W4.251.08E-50.34MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YOR336W3.904.73E-50.01KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YNL113W3.894.96E-50.03RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YLR198C_d3.875.53E-50.03YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YOR340C3.846.13E-50.04RPA43RNA polymerase I subunit A43
YBR002C3.807.32E-50.08RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YDR407C3.721.01E-40.08TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YMR033W3.641.37E-40.01ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YNL061W3.621.45E-40.01NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YGL130W3.611.53E-40.11CEG1Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR070C5.921.62E-9GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YOR013W_d5.422.99E-8IRC11_dDubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci
YJL064W_d5.403.35E-8YJL064W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1
YHR187W5.325.09E-8IKI1Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
YJL145W4.885.22E-7SFH5Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress
YBL060W4.798.24E-7YEL1Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip
YMR021C4.701.28E-6MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YDL179W4.464.16E-6PCL9Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p
YBL095W_p4.424.92E-6YBL095W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YLR131C4.376.08E-6ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YJL060W4.356.80E-6BNA3Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
YHR039C4.327.63E-6MSC7Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids
YML034W4.201.31E-5SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YER083C4.062.49E-5GET2Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
YPL081W4.032.78E-5RPS9AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1945
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1125.09E-18SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.081081160S ribosome export
0.1025.22E-15SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.082474260S ribosome export
0.0961.63E-13SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.036585460S ribosome export
0.0945.50E-13SGTC_1676st018489 23.4 μMTimTec (Natural product derivative library)36962350.050632960S ribosome export
0.0921.32E-12SGTC_403arphamenine a 50.0 μMMiscellaneous54871580.022471960S ribosome export
0.0896.86E-12SGTC_5794491-1009 110.0 μMChemDiv (Drug-like library)12710410.051948160S ribosome export
0.0881.57E-11SGTC_1952st077036 64.1 μMTimTec (Natural product derivative library)29370070.123457
0.0831.68E-10SGTC_29389059746 27.3 μMChembridge (Drug-like library)64717690.0941176fatty acid desaturase (OLE1)
0.0822.93E-10SGTC_1240136-0217 7.1 μMChemDiv (Drug-like library)39769050.0561798mitochondrial stress
0.0823.10E-10SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.024691460S ribosome export
0.0814.08E-10SGTC_1833914-0051 30.6 μMChemDiv (Drug-like library)15962320.045454560S ribosome export
0.0814.08E-10SGTC_1190121-0043 24.8 μMChemDiv (Drug-like library)10923780.037037mitochondrial stress
0.0814.86E-10SGTC_1612st002233 45.4 μMTimTec (Natural product derivative library)10350210.101124
0.0809.39E-10SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.035294160S ribosome export
0.0782.40E-9SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.10869660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1940st07599051.1 μM0.5555561084979TimTec (Natural product derivative library)391.46293.8280660S ribosome export
SGTC_1126isoteolin1.02 μM0.267606133323TimTec (Natural product library)327.374343.08325
SGTC_460trequinsin123 μM0.2666675537Miscellaneous405.489484.48604
SGTC_1855st05619539.6 μM0.2638894028379TimTec (Natural product derivative library)434.323564.2820560S ribosome export
SGTC_1083didemethylchlorpromazine sulfoxide707.39 nM0.25398788TimTec (Natural product library)353.411623.81805redox potentiating
SGTC_2790542397071.43 μM0.251376240Chembridge (Drug-like library)389.486785.09604endomembrane recycling
SGTC_1886st05735649.9 μM0.24675324207747TimTec (Natural product derivative library)400.425063.4280760S ribosome export
SGTC_2703st07785253.4 μM0.2345686281836TimTec (Natural product derivative library)374.47393.25506
SGTC_3204092-08465.34 μM0.2266671359356ChemDiv (Drug-like library)394.549664.9704ergosterol depletion effects on membrane
SGTC_10054092-06904.4 μM0.2253521529343ChemDiv (Drug-like library)364.523684.98603plasma membrane duress