st076057

N-(2-oxochromen-6-yl)-4-propan-2-ylbenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1946
Screen concentration 65.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 953009
SMILES CC(C)C1=CC=C(C=C1)C(=O)NC2=CC3=C(C=C2)OC(=O)C=C3
Standardized SMILES CC(C)c1ccc(cc1)C(=O)Nc2ccc3OC(=O)C=Cc3c2
Molecular weight 307.3432
ALogP 3.88
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.69
% growth inhibition (Hom. pool) -5.45


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 953009
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:-3.81|P-value:7.01E-5|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:DIP2(YLR129W)|FD-Score:3.94|P-value:4.02E-5|Clearance:0.4||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:MPP10(YJR002W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.4||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NUD1(YOR373W)|FD-Score:-4.23|P-value:1.19E-5|Clearance:0||SGD DESC:Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance Gene:RIB2(YOL066C)|FD-Score:3.11|P-value:9.42E-4|Clearance:0.4||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:YJR141W(YJR141W_p)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.4||SGD DESC:Essential protein of unknown function Gene:YPD1(YDL235C)|FD-Score:-3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:BMS1(YPL217C)|FD-Score:-3.81|P-value:7.01E-5|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:DIP2(YLR129W)|FD-Score:3.94|P-value:4.02E-5|Clearance:0.4||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:MPP10(YJR002W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.4||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NUD1(YOR373W)|FD-Score:-4.23|P-value:1.19E-5|Clearance:0||SGD DESC:Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance Gene:RIB2(YOL066C)|FD-Score:3.11|P-value:9.42E-4|Clearance:0.4||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:YJR141W(YJR141W_p)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.4||SGD DESC:Essential protein of unknown function Gene:YPD1(YDL235C)|FD-Score:-3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 953009
Download HOP data (tab-delimited text)  (excel)
Gene:ASR1(YPR093C)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG32(YIL146C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:BIK1(YCL029C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:CLD1(YGR110W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CST9(YLR394W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:CTI6(YPL181W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DBF2(YGR092W)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DOS2(YDR068W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:DUR3(YHL016C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ECM10(YEL030W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:GLT1(YDL171C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GYP1(YOR070C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAL9(YOL089C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:JSN1(YJR091C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:LEM3(YNL323W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MET12(YPL023C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MRS3(YJL133W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MSB4(YOL112W)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:NOT5(YPR072W)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:RIF1(YBR275C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:SAN1(YDR143C)|FD-Score:-3.98|P-value:3.44E-5||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SCO1(YBR037C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SNT2(YGL131C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TOM70(YNL121C)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UFO1(YML088W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YGL041C-B(YGL041C-B_p)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHI9(YHR029C)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YOL019W-A(YOL019W-A_p)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPK1(YKL126W)|FD-Score:-3.3|P-value:4.91E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ASR1(YPR093C)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG32(YIL146C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:BIK1(YCL029C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:CLD1(YGR110W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CST9(YLR394W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:CTI6(YPL181W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DBF2(YGR092W)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DOS2(YDR068W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:DUR3(YHL016C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ECM10(YEL030W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:GLT1(YDL171C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GYP1(YOR070C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAL9(YOL089C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:JSN1(YJR091C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:LEM3(YNL323W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MET12(YPL023C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MRS3(YJL133W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MSB4(YOL112W)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:NOT5(YPR072W)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:RIF1(YBR275C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:SAN1(YDR143C)|FD-Score:-3.98|P-value:3.44E-5||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SCO1(YBR037C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SNT2(YGL131C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TOM70(YNL121C)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UFO1(YML088W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YGL041C-B(YGL041C-B_p)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHI9(YHR029C)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YOL019W-A(YOL019W-A_p)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPK1(YKL126W)|FD-Score:-3.3|P-value:4.91E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR129W3.944.02E-50.40DIP2Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
YJR141W_p3.581.70E-40.40YJR141W_pEssential protein of unknown function
YJR002W3.452.77E-40.40MPP10Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
YOL066C3.119.42E-40.40RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YDR339C2.710.003350.17FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YLR259C2.540.005560.01HSP60Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
YPL266W2.520.005790.04DIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
YGR198W2.490.006450.04YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YBR110W2.450.007150.01ALG1Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
YKL019W2.440.007300.08RAM2Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
YER157W2.360.009050.00COG3Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YFR004W2.360.009140.05RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YDR365C2.310.010400.01ESF1Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
YPR113W2.300.010700.04PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YPR180W2.270.011700.04AOS1Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR029C4.532.97E-6YHI9Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production
YLR394W4.141.75E-5CST9SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate
YPL181W4.042.70E-5CTI6Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
YGR092W3.894.98E-5DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YOL019W-A_p3.885.29E-5YOL019W-A_pIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YBR196C-B_p3.739.42E-5YBR196C-B_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YGL131C3.651.33E-4SNT2DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
YJR091C3.641.36E-4JSN1Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl
YBR275C3.621.46E-4RIF1Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YNL323W3.581.71E-4LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
YJL133W3.571.76E-4MRS3Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
YIL146C3.492.42E-4ATG32Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane
YOR070C3.442.93E-4GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YHL016C3.413.21E-4DUR3Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
YDR068W3.383.63E-4DOS2Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

GO enrichment analysis for SGTC_1946
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1674.76E-38SGTC_1938st074717 56.4 μMTimTec (Natural product derivative library)172509790.144737
0.1322.61E-24SGTC_1948st076329 66.6 μMTimTec (Natural product derivative library)242084130.0886076
0.0741.26E-8SGTC_20585236698 133.0 μMChembridge (Fragment library)7666650.0909091
0.0691.26E-7SGTC_32019113999 49.5 μMChembridge (Drug-like library)166492240.135135
0.0655.70E-7SGTC_1924st058459 75.1 μMTimTec (Natural product derivative library)6886830.115942
0.0621.67E-6SGTC_6690108-0021 26.2 μMChemDiv (Drug-like library)172010.0701754fatty acid desaturase (OLE1)
0.0622.12E-6SGTC_1563conessine 56.1 μMTimTec (Pure natural product library)4410820.0352941
0.0571.05E-5SGTC_12201838-0075 193.0 μMChemDiv (Drug-like library)55296450.133333
0.0571.08E-5SGTC_2693st077581 40.7 μMTimTec (Natural product derivative library)252387610.157895
0.0543.65E-5SGTC_1535tetrahydropapaveroline hydrobromide 54.3 μMTimTec (Pure natural product library)27242780.056338RPP1 & pyrimidine depletion
0.0535.20E-5SGTC_13953486-0190 4.3 μMChemDiv (Drug-like library)67480790.0641026
0.0535.27E-5SGTC_28989056270 58.4 μMChembridge (Drug-like library)191546990.166667
0.0525.88E-5SGTC_282k297-0036 65.7 μMChemDiv (Drug-like library)50613780.0657895
0.0518.13E-5SGTC_277nystatin 15.5 μMMiscellaneous114687070.0727273
0.0501.42E-4SGTC_1240136-0217 7.1 μMChemDiv (Drug-like library)39769050.157895mitochondrial stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3013908013371.43 μM0.35384617050193Chembridge (Drug-like library)354.442743.64214
SGTC_1527coumarin137 μM0.347826323TimTec (Pure natural product library)146.142741.89902
SGTC_2999906976571.43 μM0.3448282097066Chembridge (Drug-like library)285.337663.30813amide catabolism
SGTC_3077911731949.47 μM0.34482817154678Chembridge (Drug-like library)285.337663.30813
SGTC_29087930301100 μM0.342223178Chembridge (Drug-like library)387.431143.00533
SGTC_1703st02698348.3 μM0.3333331104023TimTec (Natural product derivative library)414.410125.02915
SGTC_3098911903949.47 μM0.32258117161528Chembridge (Drug-like library)287.3287234.05313Golgi
SGTC_1120ficusin1.79 μM0.3207556199TimTec (Natural product library)186.163542.20303
SGTC_3004907612271.43 μM0.3174644782551Chembridge (Drug-like library)267.322383.53612
SGTC_3186911046449.47 μM0.31578917018021Chembridge (Drug-like library)269.338264.05612RPP1 & pyrimidine depletion