st076332

8-[(2-chloro-6-fluorophenyl)methylsulfanyl]-9-methyl-3H-purin-6-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1947
Screen concentration 61.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 24208416
SMILES CN1C2=C(C(=O)N=CN2)N=C1SCC3=C(C=CC=C3Cl)F
Standardized SMILES Cn1c(SCc2c(F)cccc2Cl)nc3c(O)ncnc13
Molecular weight 324.7611
ALogP 2.93
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.26
% growth inhibition (Hom. pool) -6.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24208416
Download HIP data (tab-delimited text)  (excel)
Gene:ECM16(YMR128W)|FD-Score:3.83|P-value:6.29E-5|Clearance:0.07||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:ERG12(YMR208W)|FD-Score:3.92|P-value:4.45E-5|Clearance:0.08||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG29(YMR134W_p)|FD-Score:-3.27|P-value:5.40E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:ERO1(YML130C)|FD-Score:-3.13|P-value:8.59E-4|Clearance:0||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:EXO70(YJL085W)|FD-Score:3.76|P-value:8.51E-5|Clearance:0.16||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FRS2(YFL022C)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.28||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GPI8(YDR331W)|FD-Score:3.76|P-value:8.43E-5|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:PMI40(YER003C)|FD-Score:5.53|P-value:1.63E-8|Clearance:1.61||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PTI1(YGR156W)|FD-Score:3.27|P-value:5.45E-4|Clearance:0.19||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:PWP1(YLR196W)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RRS1(YOR294W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.06||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:ECM16(YMR128W)|FD-Score:3.83|P-value:6.29E-5|Clearance:0.07||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:ERG12(YMR208W)|FD-Score:3.92|P-value:4.45E-5|Clearance:0.08||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG29(YMR134W_p)|FD-Score:-3.27|P-value:5.40E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:ERO1(YML130C)|FD-Score:-3.13|P-value:8.59E-4|Clearance:0||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:EXO70(YJL085W)|FD-Score:3.76|P-value:8.51E-5|Clearance:0.16||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FRS2(YFL022C)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.28||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GPI8(YDR331W)|FD-Score:3.76|P-value:8.43E-5|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:PMI40(YER003C)|FD-Score:5.53|P-value:1.63E-8|Clearance:1.61||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PTI1(YGR156W)|FD-Score:3.27|P-value:5.45E-4|Clearance:0.19||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:PWP1(YLR196W)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RRS1(YOR294W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.06||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24208416
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ACS1(YAL054C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AIM46(YHR199C)|FD-Score:5.3|P-value:5.68E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARL1(YBR164C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARR1(YPR199C)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATE1(YGL017W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:BIO4(YNR057C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:CIR1(YGR207C)|FD-Score:-3.72|P-value:9.80E-5||SGD DESC:Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response Gene:CRZ1(YNL027W)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress Gene:CTF18(YMR078C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CYT1(YOR065W)|FD-Score:-4.79|P-value:8.24E-7||SGD DESC:Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex Gene:DBF2(YGR092W)|FD-Score:5.24|P-value:8.06E-8||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DLS1(YJL065C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication Gene:DPL1(YDR294C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Gene:DSS4(YPR017C)|FD-Score:3.29|P-value:5.10E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM22(YLR228C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:5.25|P-value:7.72E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FCY2(YER056C)|FD-Score:-4.91|P-value:4.48E-7||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FTH1(YBR207W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress Gene:GAL11(YOL051W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GPR1(YDL035C)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HOM2(YDR158W)|FD-Score:4.83|P-value:6.71E-7||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:ICY1(YMR195W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IDP3(YNL009W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IME1(YJR094C)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:IMP1(YMR150C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IOC4(YMR044W)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:MET14(YKL001C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MND2(YIR025W)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPL33(YMR286W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC6(YOR354C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MSS51(YLR203C)|FD-Score:-3.8|P-value:7.14E-5||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MUB1(YMR100W)|FD-Score:-5.75|P-value:4.44E-9||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:MXR2(YCL033C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NDE2(YDL085W)|FD-Score:-4.32|P-value:7.94E-6||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NNK1(YKL171W)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm Gene:NPC2(YDL046W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:PET54(YGR222W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PIR1(YKL164C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PMA2(YPL036W)|FD-Score:-3.72|P-value:9.89E-5||SGD DESC:Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential Gene:PRR1(YKL116C)|FD-Score:-5.35|P-value:4.40E-8||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:RGT1(YKL038W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RNP1(YLL046C)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL24B(YGR148C)|FD-Score:4.76|P-value:9.72E-7||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL37A(YLR185W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication Gene:RRT6(YGL146C_p)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:RUB1(YDR139C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SAP185(YJL098W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication Gene:SAT4(YCR008W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SEG2(YKL105C_p)|FD-Score:-4.56|P-value:2.54E-6||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SEH1(YGL100W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:STB6(YKL072W)|FD-Score:-3.89|P-value:4.93E-5||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:TDA6(YPR157W_p)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TGS1(YPL157W)|FD-Score:4.27|P-value:9.56E-6||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOM70(YNL121C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:4.49|P-value:3.56E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TPO5(YKL174C)|FD-Score:-3.8|P-value:7.36E-5||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:UBP2(YOR124C)|FD-Score:-3.79|P-value:7.55E-5||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:UBX2(YML013W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UPF3(YGR072W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VPS4(YPR173C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCK2(YNL154C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YCL023C(YCL023C_d)|FD-Score:-3.73|P-value:9.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YDL011C(YDL011C_d)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR056C(YDR056C_p)|FD-Score:-5.64|P-value:8.58E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YER034W(YER034W_p)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YGL114W(YGL114W_p)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YGR064W(YGR064W_d)|FD-Score:4.57|P-value:2.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHR097C(YHR097C_p)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YJL160C(YJL160C_p)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YML018C(YML018C_p)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML083C(YML083C_p)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YMR153C-A(YMR153C-A_d)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YMR210W(YMR210W)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Putative protein of unknown function Gene:YMR320W(YMR320W_d)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOR020W-A(YOR020W-A_p)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL107W(YPL107W_p)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPL272C(YPL272C_p)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:YPR050C(YPR050C_d)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR089W(YPR089W_p)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR127W(YPR127W)|FD-Score:6.9|P-value:2.63E-12||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:AAC3(YBR085W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ACS1(YAL054C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AIM46(YHR199C)|FD-Score:5.3|P-value:5.68E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARL1(YBR164C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARR1(YPR199C)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATE1(YGL017W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:BIO4(YNR057C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:CIR1(YGR207C)|FD-Score:-3.72|P-value:9.80E-5||SGD DESC:Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response Gene:CRZ1(YNL027W)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress Gene:CTF18(YMR078C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CYT1(YOR065W)|FD-Score:-4.79|P-value:8.24E-7||SGD DESC:Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex Gene:DBF2(YGR092W)|FD-Score:5.24|P-value:8.06E-8||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DLS1(YJL065C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication Gene:DPL1(YDR294C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Gene:DSS4(YPR017C)|FD-Score:3.29|P-value:5.10E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM22(YLR228C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:5.25|P-value:7.72E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FCY2(YER056C)|FD-Score:-4.91|P-value:4.48E-7||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FTH1(YBR207W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress Gene:GAL11(YOL051W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GPR1(YDL035C)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HOM2(YDR158W)|FD-Score:4.83|P-value:6.71E-7||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:ICY1(YMR195W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IDP3(YNL009W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IME1(YJR094C)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:IMP1(YMR150C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IOC4(YMR044W)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:MET14(YKL001C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MND2(YIR025W)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPL33(YMR286W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC6(YOR354C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MSS51(YLR203C)|FD-Score:-3.8|P-value:7.14E-5||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MUB1(YMR100W)|FD-Score:-5.75|P-value:4.44E-9||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:MXR2(YCL033C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NDE2(YDL085W)|FD-Score:-4.32|P-value:7.94E-6||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NNK1(YKL171W)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm Gene:NPC2(YDL046W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:PET54(YGR222W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PIR1(YKL164C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PMA2(YPL036W)|FD-Score:-3.72|P-value:9.89E-5||SGD DESC:Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential Gene:PRR1(YKL116C)|FD-Score:-5.35|P-value:4.40E-8||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:RGT1(YKL038W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RNP1(YLL046C)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL24B(YGR148C)|FD-Score:4.76|P-value:9.72E-7||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL37A(YLR185W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication Gene:RRT6(YGL146C_p)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:RUB1(YDR139C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SAP185(YJL098W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication Gene:SAT4(YCR008W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SEG2(YKL105C_p)|FD-Score:-4.56|P-value:2.54E-6||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SEH1(YGL100W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:STB6(YKL072W)|FD-Score:-3.89|P-value:4.93E-5||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:TDA6(YPR157W_p)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TGS1(YPL157W)|FD-Score:4.27|P-value:9.56E-6||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOM70(YNL121C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:4.49|P-value:3.56E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TPO5(YKL174C)|FD-Score:-3.8|P-value:7.36E-5||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:UBP2(YOR124C)|FD-Score:-3.79|P-value:7.55E-5||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:UBX2(YML013W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UPF3(YGR072W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VPS4(YPR173C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCK2(YNL154C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; YCK2 has a paralog, YCK1, that arose from the whole genome duplication Gene:YCL023C(YCL023C_d)|FD-Score:-3.73|P-value:9.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YDL011C(YDL011C_d)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR056C(YDR056C_p)|FD-Score:-5.64|P-value:8.58E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YER034W(YER034W_p)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YGL114W(YGL114W_p)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YGR064W(YGR064W_d)|FD-Score:4.57|P-value:2.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHR097C(YHR097C_p)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YJL160C(YJL160C_p)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YML018C(YML018C_p)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML083C(YML083C_p)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YMR153C-A(YMR153C-A_d)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YMR210W(YMR210W)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Putative protein of unknown function Gene:YMR320W(YMR320W_d)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOR020W-A(YOR020W-A_p)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL107W(YPL107W_p)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPL272C(YPL272C_p)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:YPR050C(YPR050C_d)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR089W(YPR089W_p)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR127W(YPR127W)|FD-Score:6.9|P-value:2.63E-12||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER003C5.531.63E-81.61PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YMR208W3.924.45E-50.08ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YMR128W3.836.29E-50.07ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YDR331W3.768.43E-50.00GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YJL085W3.768.51E-50.16EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YOR294W3.601.58E-40.06RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YFL022C3.541.99E-40.28FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YGR156W3.275.45E-40.19PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YDR362C3.070.001060.14TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YBL014C2.940.001670.03RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YML093W2.910.001820.07UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDL058W2.840.002240.15USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YGR065C2.690.003600.04VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
YBR140C2.640.004100.01IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YER147C2.630.004240.02SCC4Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR127W6.902.63E-12YPR127WPutative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YHR199C5.305.68E-8AIM46Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YMR202W5.257.72E-8ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YGR092W5.248.06E-8DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YNR057C5.191.03E-7BIO4Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels
YDR158W4.836.71E-7HOM2Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YGR148C4.769.72E-7RPL24BRibosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication
YML013W4.731.12E-6UBX2Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
YGR064W_d4.572.40E-6YGR064W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C
YPR050C_d4.523.09E-6YPR050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
YOR273C4.493.56E-6TPO4Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
YPL157W4.279.56E-6TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YMR242W-A_p4.171.51E-5YMR242W-A_pPutative protein of unknown function
YPR157W_p3.855.99E-5TDA6_pPutative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele
YML018C_p3.846.23E-5YML018C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1947
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2342.25E-74SGTC_1943st076331 65.2 μMTimTec (Natural product derivative library)242084150.703704
0.1323.37E-24SGTC_1107amlodipine 47.0 μMNIH Clinical Collection21620.12766
0.1194.64E-20SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0657895DNA intercalators
0.1141.74E-18SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0326087DNA intercalators
0.1111.61E-17SGTC_7504073-0104 132.0 μMChemDiv (Drug-like library)223309570.142857
0.1102.72E-17SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.121212DNA intercalators
0.1088.63E-17SGTC_412gemfibrozil 100.0 μMMiscellaneous34630.0769231DNA intercalators
0.1062.41E-16SGTC_469w7 172.0 μMICCB bioactive library451155700.140845ERG2
0.0961.16E-13SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0724638Golgi
0.0952.03E-13SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.0985916DNA intercalators
0.0921.18E-12SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.139241ERG2
0.0921.18E-12SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.0882353DNA intercalators
0.0905.18E-12SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0666667ERG2
0.0889.70E-12SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.104651ERG2
0.0881.22E-11SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.0930233ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1943st07633165.2 μM0.70370424208415TimTec (Natural product derivative library)306.770642.72813
SGTC_1942st07633065.2 μM0.63636424208414TimTec (Natural product derivative library)306.770642.72813
SGTC_1948st07632966.6 μM0.5524208413TimTec (Natural product derivative library)300.378743.03613
SGTC_1944st07637156.9 μM0.50746324208455TimTec (Natural product derivative library)351.3312461.52726
SGTC_2707st07855666.6 μM0.44444417250899TimTec (Natural product derivative library)300.378742.89813
SGTC_2708st07855956 μM0.37837817250902TimTec (Natural product derivative library)357.430062.43724
SGTC_2710st07856253.72 μM0.36486517250908TimTec (Natural product derivative library)357.430062.40724
SGTC_2709st07856056 μM0.34210517250903TimTec (Natural product derivative library)357.430062.43724
SGTC_2692st07742647.7 μM0.33333325238571TimTec (Natural product derivative library)419.499444.12124
SGTC_1029k015-003064.7 μM0.2923082208316ChemDiv (Drug-like library)289.6888633.32903