sophoraflavanone G

(2S)-2-(2,4-dihydroxyphenyl)-5,7-dihydroxy-8-(5-methyl-2-prop-1-en-2-ylhex-4-enyl)-2,3-dihydrochromen-4-one

Sophoraflavanone G is a volatile phytoncide; a natural flavanone with antimicrobial activity.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1951
Screen concentration 9.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 9910234
SMILES CC(=CCC(CC1=C(C=C(C2=C1OC(CC2=O)C3=C(C=C(C=C3)O)O)O)O)C(=C)C)C
Standardized SMILES CC(=CCC(Cc1c(O)cc(O)c2C(=O)CC(Oc12)c3ccc(O)cc3O)C(=C)C)C
Molecular weight 424.4862
ALogP 5.66
H-bond donor count 4
H-bond acceptor count 6
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 30.01
% growth inhibition (Hom. pool) 5.76


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 9910234
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.74||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CAB2(YIL083C)|FD-Score:3.1|P-value:9.82E-4|Clearance:0.04||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:DBP2(YNL112W)|FD-Score:-3.15|P-value:8.24E-4|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:IRA1(YBR140C)|FD-Score:3.85|P-value:6.01E-5|Clearance:0.39||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:NUP159(YIL115C)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:OLE1(YGL055W)|FD-Score:3.94|P-value:4.16E-5|Clearance:0.09||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PRE10(YOR362C)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.12||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP6(YBR055C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.74||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PUP3(YER094C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.03||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RGR1(YLR071C)|FD-Score:-3.6|P-value:1.57E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation Gene:RIA1(YNL163C)|FD-Score:3.34|P-value:4.23E-4|Clearance:0.08||SGD DESC:Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes Gene:RRP43(YCR035C)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SLD5(YDR489W)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.02||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:TFG1(YGR186W)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.03||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:YDR053W(YDR053W_d)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.08||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YOR146W(YOR146W_d)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ACC1(YNR016C)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.74||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CAB2(YIL083C)|FD-Score:3.1|P-value:9.82E-4|Clearance:0.04||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:DBP2(YNL112W)|FD-Score:-3.15|P-value:8.24E-4|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:IRA1(YBR140C)|FD-Score:3.85|P-value:6.01E-5|Clearance:0.39||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:NUP159(YIL115C)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:OLE1(YGL055W)|FD-Score:3.94|P-value:4.16E-5|Clearance:0.09||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PRE10(YOR362C)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.12||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP6(YBR055C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.74||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PUP3(YER094C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.03||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RGR1(YLR071C)|FD-Score:-3.6|P-value:1.57E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation Gene:RIA1(YNL163C)|FD-Score:3.34|P-value:4.23E-4|Clearance:0.08||SGD DESC:Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes Gene:RRP43(YCR035C)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SLD5(YDR489W)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.02||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:TFG1(YGR186W)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.03||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:YDR053W(YDR053W_d)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.08||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YOR146W(YOR146W_d)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 9910234
Download HOP data (tab-delimited text)  (excel)
Gene:ADH3(YMR083W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:APL5(YPL195W)|FD-Score:7.05|P-value:9.16E-13||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APS1(YLR170C)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARF2(YDL137W)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARL1(YBR164C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:AXL2(YIL140W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:BEM4(YPL161C)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BRP1(YGL007W_d)|FD-Score:4.82|P-value:7.07E-7||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CCW12(YLR110C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHL4(YDR254W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:CKB1(YGL019W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:COG5(YNL051W)|FD-Score:-3.11|P-value:9.30E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-5.19|P-value:1.03E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRH1(YGR189C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CRT10(YOL063C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CSM4(YPL200W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CTR9(YOL145C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DMA1(YHR115C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS4(YPR017C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EAF5(YEL018W)|FD-Score:5.3|P-value:5.84E-8||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:ERG2(YMR202W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERJ5(YFR041C)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAA4(YMR246W)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FUR4(YBR021W)|FD-Score:6.29|P-value:1.61E-10||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN5(YGR252W)|FD-Score:4.06|P-value:2.51E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GLY1(YEL046C)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GTR2(YGR163W)|FD-Score:-3.79|P-value:7.53E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GYP1(YOR070C)|FD-Score:-6.58|P-value:2.30E-11||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBS1(YKR084C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HEL1(YKR017C_p)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HTD2(YHR067W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXK1(YFR053C)|FD-Score:5.04|P-value:2.28E-7||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-5.53|P-value:1.61E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KRE6(YPR159W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LCL1(YPL056C_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LDB7(YBL006C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LSC2(YGR244C)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:LSM6(YDR378C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MEF2(YJL102W)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MFT1(YML062C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MHF2(YDL160C-A)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MMS2(YGL087C)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL6(YHR147C)|FD-Score:-8.42|P-value:1.83E-17||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSB3(YNL293W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSN4(YKL062W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MTM1(YGR257C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NDT80(YHR124W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NPL4(YBR170C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:NPP2(YEL016C)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:NTO1(YPR031W)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:NUP170(YBL079W)|FD-Score:-3.83|P-value:6.44E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:-3.83|P-value:6.30E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH2(YDL019C)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCL9(YDL179W)|FD-Score:-5.31|P-value:5.40E-8||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PDR10(YOR328W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PET54(YGR222W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PHO90(YJL198W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PIF1(YML061C)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PMP2(YEL017C-A)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PRM10(YJL108C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRO2(YOR323C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS3(YHL011C)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK2(YJR059W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTP2(YOR208W)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:QCR7(YDR529C)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RBK1(YCR036W)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Putative ribokinase Gene:REG1(YDR028C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RKM2(YDR198C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:ROY1(YMR258C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RRT2(YBR246W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RTC1(YOL138C)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RTC3(YHR087W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAP1(YER047C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SET2(YJL168C)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SIP4(YJL089W)|FD-Score:4.92|P-value:4.35E-7||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SLC1(YDL052C)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SNL1(YIL016W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SPO21(YOL091W)|FD-Score:-5.58|P-value:1.21E-8||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPT3(YDR392W)|FD-Score:-4.11|P-value:1.97E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRC1(YML034W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSN2(YDR443C)|FD-Score:-3.15|P-value:8.27E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SUT2(YPR009W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TAT2(YOL020W)|FD-Score:-8.05|P-value:4.10E-16||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:THR1(YHR025W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TPN1(YGL186C)|FD-Score:-4.94|P-value:3.98E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM732(YMR259C_p)|FD-Score:-3.87|P-value:5.46E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:VPS21(YOR089C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:-4.63|P-value:1.85E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS53(YJL029C)|FD-Score:-5.43|P-value:2.82E-8||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:VPS61(YDR136C_d)|FD-Score:-6.71|P-value:9.63E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:WTM2(YOR229W)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YCP4(YCR004C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR061W(YCR061W_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR290W(YDR290W_d)|FD-Score:4.29|P-value:9.13E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR306C(YDR306C_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR445C(YDR445C_d)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL045C(YEL045C_d)|FD-Score:-5.11|P-value:1.58E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER087C-A(YER087C-A_d)|FD-Score:-4.29|P-value:9.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YER184C(YER184C_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YGR111W(YGR111W_p)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR146C-A(YGR146C-A_p)|FD-Score:-4.52|P-value:3.05E-6||SGD DESC:Putative protein of unknown function Gene:YGR226C(YGR226C_d)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YHR127W(YHR127W)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YHR130C(YHR130C_d)|FD-Score:4.51|P-value:3.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL029C(YIL029C_p)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL086C(YIL086C_d)|FD-Score:4.29|P-value:9.13E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR011C(YKR011C_p)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR252W(YLR252W_d)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR271W(YLR271W_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR307C-A(YLR307C-A_p)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:-3.76|P-value:8.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR075C-A(YMR075C-A_d)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL194C(YNL194C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YOL024W(YOL024W_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YPL199C(YPL199C_p)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR114W(YPR114W_p)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Putative protein of unknown function Gene:YPR123C(YPR123C_d)|FD-Score:4.88|P-value:5.42E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP2(YDR326C)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ADH3(YMR083W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:APL5(YPL195W)|FD-Score:7.05|P-value:9.16E-13||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APS1(YLR170C)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARF2(YDL137W)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARL1(YBR164C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:AXL2(YIL140W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:BEM4(YPL161C)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BRP1(YGL007W_d)|FD-Score:4.82|P-value:7.07E-7||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CCW12(YLR110C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHL4(YDR254W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:CKB1(YGL019W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:COG5(YNL051W)|FD-Score:-3.11|P-value:9.30E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-5.19|P-value:1.03E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRH1(YGR189C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CRT10(YOL063C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CSM4(YPL200W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CTR9(YOL145C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DMA1(YHR115C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS4(YPR017C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EAF5(YEL018W)|FD-Score:5.3|P-value:5.84E-8||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:ERG2(YMR202W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERJ5(YFR041C)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAA4(YMR246W)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FUR4(YBR021W)|FD-Score:6.29|P-value:1.61E-10||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN5(YGR252W)|FD-Score:4.06|P-value:2.51E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GLY1(YEL046C)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GTR2(YGR163W)|FD-Score:-3.79|P-value:7.53E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GYP1(YOR070C)|FD-Score:-6.58|P-value:2.30E-11||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBS1(YKR084C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HEL1(YKR017C_p)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HTD2(YHR067W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXK1(YFR053C)|FD-Score:5.04|P-value:2.28E-7||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-5.53|P-value:1.61E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KRE6(YPR159W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LCL1(YPL056C_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LDB7(YBL006C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LSC2(YGR244C)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:LSM6(YDR378C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MEF2(YJL102W)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MFT1(YML062C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MHF2(YDL160C-A)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MMS2(YGL087C)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL6(YHR147C)|FD-Score:-8.42|P-value:1.83E-17||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSB3(YNL293W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSN4(YKL062W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MTM1(YGR257C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NDT80(YHR124W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NPL4(YBR170C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:NPP2(YEL016C)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:NTO1(YPR031W)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:NUP170(YBL079W)|FD-Score:-3.83|P-value:6.44E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:-3.83|P-value:6.30E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH2(YDL019C)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCL9(YDL179W)|FD-Score:-5.31|P-value:5.40E-8||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PDR10(YOR328W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PET54(YGR222W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PHO90(YJL198W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PIF1(YML061C)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PMP2(YEL017C-A)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PRM10(YJL108C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRO2(YOR323C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS3(YHL011C)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK2(YJR059W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTP2(YOR208W)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:QCR7(YDR529C)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RBK1(YCR036W)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Putative ribokinase Gene:REG1(YDR028C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RKM2(YDR198C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:ROY1(YMR258C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RRT2(YBR246W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RTC1(YOL138C)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RTC3(YHR087W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAP1(YER047C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SET2(YJL168C)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SIP4(YJL089W)|FD-Score:4.92|P-value:4.35E-7||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SLC1(YDL052C)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SNL1(YIL016W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SPO21(YOL091W)|FD-Score:-5.58|P-value:1.21E-8||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPT3(YDR392W)|FD-Score:-4.11|P-value:1.97E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRC1(YML034W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSN2(YDR443C)|FD-Score:-3.15|P-value:8.27E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SUT2(YPR009W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TAT2(YOL020W)|FD-Score:-8.05|P-value:4.10E-16||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:THR1(YHR025W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TPN1(YGL186C)|FD-Score:-4.94|P-value:3.98E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM732(YMR259C_p)|FD-Score:-3.87|P-value:5.46E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:VPS21(YOR089C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:-4.63|P-value:1.85E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS53(YJL029C)|FD-Score:-5.43|P-value:2.82E-8||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:VPS61(YDR136C_d)|FD-Score:-6.71|P-value:9.63E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:WTM2(YOR229W)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YCP4(YCR004C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR061W(YCR061W_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR290W(YDR290W_d)|FD-Score:4.29|P-value:9.13E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR306C(YDR306C_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR445C(YDR445C_d)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL045C(YEL045C_d)|FD-Score:-5.11|P-value:1.58E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER087C-A(YER087C-A_d)|FD-Score:-4.29|P-value:9.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YER184C(YER184C_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YGR111W(YGR111W_p)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR146C-A(YGR146C-A_p)|FD-Score:-4.52|P-value:3.05E-6||SGD DESC:Putative protein of unknown function Gene:YGR226C(YGR226C_d)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YHR127W(YHR127W)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YHR130C(YHR130C_d)|FD-Score:4.51|P-value:3.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL029C(YIL029C_p)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL086C(YIL086C_d)|FD-Score:4.29|P-value:9.13E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR011C(YKR011C_p)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR252W(YLR252W_d)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR271W(YLR271W_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR307C-A(YLR307C-A_p)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:-3.76|P-value:8.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR075C-A(YMR075C-A_d)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL194C(YNL194C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YOL024W(YOL024W_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YPL199C(YPL199C_p)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR114W(YPR114W_p)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Putative protein of unknown function Gene:YPR123C(YPR123C_d)|FD-Score:4.88|P-value:5.42E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP2(YDR326C)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YNR016C4.751.03E-60.74ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YBR055C4.681.46E-60.74PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YGL055W3.944.16E-50.09OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YBR140C3.856.01E-50.39IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YOR362C3.462.75E-40.12PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YNL163C3.344.23E-40.08RIA1Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
YGR186W3.265.58E-40.03TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YDR489W3.236.19E-40.02SLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YDR053W_d3.216.68E-40.08YDR053W_dPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
YER094C3.138.89E-40.03PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YIL083C3.109.82E-40.04CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YMR268C3.050.001140.02PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YGL030W3.030.001231.93E-5RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YNL039W3.030.001230.12BDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
YPR165W2.910.001830.08RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL195W7.059.16E-13APL5Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
YBR021W6.291.61E-10FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YEL018W5.305.84E-8EAF5Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex
YOL138C5.141.36E-7RTC1Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif
YFR053C5.042.28E-7HXK1Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
YGR226C_d4.983.14E-7YGR226C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
YJL089W4.924.35E-7SIP4C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus
YPR123C_d4.885.42E-7YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YGL007W_d4.827.07E-7BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YJL198W4.691.39E-6PHO90Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication
YMR246W4.552.74E-6FAA4Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication
YKL062W4.533.00E-6MSN4Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication
YHR130C_d4.513.30E-6YHR130C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR290W_d4.299.13E-6YDR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
YIL086C_d4.299.13E-6YIL086C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1951
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1842.40E-46SGTC_1950st077106 7.2 μMTimTec (Natural product derivative library)138318640.78125RNA processing & uracil transport
0.1301.63E-23SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.0588235RNA processing & uracil transport
0.1223.83E-21SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.0722892RNA processing & uracil transport
0.1196.41E-20SGTC_22206782180 200.0 μMChembridge (Fragment library)8338030.0833333RNA processing & uracil transport
0.1132.85E-18SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.0681818RNA processing & uracil transport
0.1054.70E-16SGTC_398bronopol 33.3 μMMiscellaneous24500.0422535RNA processing & uracil transport
0.1001.19E-14SGTC_9231900-2434 73.3 μMChemDiv (Drug-like library)59105700.0729167RNA processing & uracil transport
0.0985.29E-14SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.0722892RNA processing & uracil transport
0.0881.31E-11SGTC_21925915882 198.5 μMChembridge (Fragment library)28761490.0987654RNA processing & uracil transport
0.0832.06E-10SGTC_3051682-2595 20.8 μMChemDiv (Drug-like library)2260330.0595238RNA processing & uracil transport
0.0791.53E-9SGTC_1745st081495 74.0 μMTimTec (Natural product derivative library)138424020.230769RNA processing & uracil transport
0.0781.62E-9SGTC_224benfluorex 43.9 μMMiscellaneous23180.107527
0.0773.95E-9SGTC_32589138477 49.5 μMChembridge (Drug-like library)49703550.0769231
0.0756.71E-9SGTC_1555citronellol 128.0 μMTimTec (Pure natural product library)88420.144928
0.0722.75E-8SGTC_1974st069448 26.9 μMTimTec (Natural product derivative library)63235870.0674157

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1950st0771067.18 μM0.7812513831864TimTec (Natural product derivative library)408.486825.90635RNA processing & uracil transport
SGTC_918glabranin770.73 nM0.514706124049TimTec (Natural product library)324.37044.47224
SGTC_15372',6'-dihydroxyflavanone78 μM0.3285715106787TimTec (Pure natural product library)256.253382.61524
SGTC_1889st0590805.18 μM0.271429176925TimTec (Natural product derivative library)240.253982.85713
SGTC_1123mangostin811.29 nM0.255281650TimTec (Natural product library)410.459645.93536mitochondrial response to ROS
SGTC_1923st05845819.1 μM0.2432433534982TimTec (Natural product derivative library)240.253982.85713
SGTC_16014'-hydroxyflavanone55.5 μM0.236111165506TimTec (Pure natural product library)240.253982.85713RPP1 & pyrimidine depletion
SGTC_1745st08149574 μM0.23076913842402TimTec (Natural product derivative library)270.279962.94814RNA processing & uracil transport
SGTC_1562morin hydrate59.1 μM0.21621618542136TimTec (Pure natural product library)338.266261.21679
SGTC_2518prenyletin46.28 μM0.215193873459Microsource (Natural product library)246.258563.10614