st077036

ethyl 8-methyl-1,3,4,5-tetrahydropyrido[4,3-b]indole-2-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1952
Screen concentration 64.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 2937007
SMILES CCOC(=O)N1CCC2=C(C1)C3=C(N2)C=CC(=C3)C
Standardized SMILES CCOC(=O)N1CCc2[nH]c3ccc(C)cc3c2C1
Molecular weight 258.3156
ALogP 3.01
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.52
% growth inhibition (Hom. pool) 3.49


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2937007
Download HIP data (tab-delimited text)  (excel)
Gene:ALG13(YGL047W)|FD-Score:4.93|P-value:4.13E-7|Clearance:0.16||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ARP9(YMR033W)|FD-Score:5.36|P-value:4.07E-8|Clearance:0.04||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:HYP2(YEL034W)|FD-Score:3.73|P-value:9.54E-5|Clearance:0.39||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MTR3(YGR158C)|FD-Score:-3.42|P-value:3.08E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NUP49(YGL172W)|FD-Score:-3.42|P-value:3.08E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:OLE1(YGL055W)|FD-Score:3.99|P-value:3.34E-5|Clearance:0.08||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PDI1(YCL043C)|FD-Score:3.89|P-value:4.96E-5|Clearance:0.16||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PRP6(YBR055C)|FD-Score:6.26|P-value:1.90E-10|Clearance:0.55||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PUP1(YOR157C)|FD-Score:-4.59|P-value:2.22E-6|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RIM2(YBR192W)|FD-Score:5.72|P-value:5.44E-9|Clearance:0.35||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RIX1(YHR197W)|FD-Score:4|P-value:3.11E-5|Clearance:0.02||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RNT1(YMR239C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.21||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RPC19(YNL113W)|FD-Score:-3.15|P-value:8.15E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL10(YLR075W)|FD-Score:-3.87|P-value:5.35E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPM2(YML091C)|FD-Score:4.96|P-value:3.60E-7|Clearance:0.03||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPS20(YHL015W)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RSC8(YFR037C)|FD-Score:5.32|P-value:5.14E-8|Clearance:0.37||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RTS2(YOR077W)|FD-Score:-3.12|P-value:9.04E-4|Clearance:0||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SGD1(YLR336C)|FD-Score:3.13|P-value:8.65E-4|Clearance:0.04||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SHQ1(YIL104C)|FD-Score:-4.07|P-value:2.39E-5|Clearance:0||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SLY1(YDR189W)|FD-Score:-3.31|P-value:4.74E-4|Clearance:0||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:SPC105(YGL093W)|FD-Score:4.77|P-value:9.43E-7|Clearance:0.47||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:STH1(YIL126W)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.18||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUI3(YPL237W)|FD-Score:-4.72|P-value:1.18E-6|Clearance:0||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TAM41(YGR046W)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.02||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TOM40(YMR203W)|FD-Score:7.8|P-value:3.02E-15|Clearance:1.54||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:VTI1(YMR197C)|FD-Score:3.91|P-value:4.68E-5|Clearance:0.01||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL196W(YDL196W_d)|FD-Score:9.36|P-value:3.81E-21|Clearance:1.56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGL069C(YGL069C_d)|FD-Score:4.29|P-value:8.82E-6|Clearance:0.11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YPL238C(YPL238C_d)|FD-Score:-3.24|P-value:6.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ALG13(YGL047W)|FD-Score:4.93|P-value:4.13E-7|Clearance:0.16||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ARP9(YMR033W)|FD-Score:5.36|P-value:4.07E-8|Clearance:0.04||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:HYP2(YEL034W)|FD-Score:3.73|P-value:9.54E-5|Clearance:0.39||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MTR3(YGR158C)|FD-Score:-3.42|P-value:3.08E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NUP49(YGL172W)|FD-Score:-3.42|P-value:3.08E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:OLE1(YGL055W)|FD-Score:3.99|P-value:3.34E-5|Clearance:0.08||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PDI1(YCL043C)|FD-Score:3.89|P-value:4.96E-5|Clearance:0.16||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PRP6(YBR055C)|FD-Score:6.26|P-value:1.90E-10|Clearance:0.55||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PUP1(YOR157C)|FD-Score:-4.59|P-value:2.22E-6|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RIM2(YBR192W)|FD-Score:5.72|P-value:5.44E-9|Clearance:0.35||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RIX1(YHR197W)|FD-Score:4|P-value:3.11E-5|Clearance:0.02||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RNT1(YMR239C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.21||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RPC19(YNL113W)|FD-Score:-3.15|P-value:8.15E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL10(YLR075W)|FD-Score:-3.87|P-value:5.35E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPM2(YML091C)|FD-Score:4.96|P-value:3.60E-7|Clearance:0.03||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPS20(YHL015W)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RSC8(YFR037C)|FD-Score:5.32|P-value:5.14E-8|Clearance:0.37||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RTS2(YOR077W)|FD-Score:-3.12|P-value:9.04E-4|Clearance:0||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SGD1(YLR336C)|FD-Score:3.13|P-value:8.65E-4|Clearance:0.04||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SHQ1(YIL104C)|FD-Score:-4.07|P-value:2.39E-5|Clearance:0||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SLY1(YDR189W)|FD-Score:-3.31|P-value:4.74E-4|Clearance:0||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:SPC105(YGL093W)|FD-Score:4.77|P-value:9.43E-7|Clearance:0.47||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:STH1(YIL126W)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.18||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUI3(YPL237W)|FD-Score:-4.72|P-value:1.18E-6|Clearance:0||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TAM41(YGR046W)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.02||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TOM40(YMR203W)|FD-Score:7.8|P-value:3.02E-15|Clearance:1.54||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:VTI1(YMR197C)|FD-Score:3.91|P-value:4.68E-5|Clearance:0.01||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL196W(YDL196W_d)|FD-Score:9.36|P-value:3.81E-21|Clearance:1.56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGL069C(YGL069C_d)|FD-Score:4.29|P-value:8.82E-6|Clearance:0.11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YPL238C(YPL238C_d)|FD-Score:-3.24|P-value:6.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2937007
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:4.4|P-value:5.42E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ANT1(YPR128C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARO1(YDR127W)|FD-Score:5.21|P-value:9.58E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:AVO2(YMR068W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BRE1(YDL074C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BTS1(YPL069C)|FD-Score:-4.94|P-value:3.85E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CIN8(YEL061C)|FD-Score:4.41|P-value:5.12E-6||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COG6(YNL041C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX10(YPL172C)|FD-Score:6.99|P-value:1.36E-12||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:COX8(YLR395C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUR1(YPR158W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DCG1(YIR030C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DDR2(YOL052C-A)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DFG5(YMR238W)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DIN7(YDR263C)|FD-Score:-3.94|P-value:4.04E-5||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DRS2(YAL026C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSF2(YBR007C_p)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EFG1(YGR271C-A)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELA1(YNL230C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ERV14(YGL054C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EXO1(YOR033C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FBP1(YLR377C)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FCY2(YER056C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FPR2(YDR519W)|FD-Score:-3.75|P-value:8.92E-5||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GEP4(YHR100C)|FD-Score:4.86|P-value:5.75E-7||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIP3(YPL137C)|FD-Score:4.8|P-value:8.06E-7||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GPR1(YDL035C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GYP1(YOR070C)|FD-Score:6|P-value:9.71E-10||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOM2(YDR158W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSP42(YDR171W)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IMA1(YGR287C)|FD-Score:-5.7|P-value:6.05E-9||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:JNM1(YMR294W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KCS1(YDR017C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LNP1(YHR192W_p)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MAC1(YMR021C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDG1(YNL173C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MRPL6(YHR147C)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB4(YOL112W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MST1(YKL194C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MSY1(YPL097W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MTM1(YGR257C)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NAT5(YOR253W)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NOP12(YOL041C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NUP2(YLR335W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OM14(YBR230C)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OXR1(YPL196W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PCK1(YKR097W)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PEP8(YJL053W)|FD-Score:4.35|P-value:6.88E-6||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX28(YHR150W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:POP2(YNR052C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PST1(YDR055W)|FD-Score:-3.79|P-value:7.61E-5||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PUS7(YOR243C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RIM13(YMR154C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:6.02|P-value:8.69E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS23A(YGR118W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RTC3(YHR087W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SIZ1(YDR409W)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLO1(YER180C-A)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:STB2(YMR053C)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TKL1(YPR074C)|FD-Score:-4.28|P-value:9.30E-6||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:4.84|P-value:6.49E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:12.3|P-value:3.81E-35||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.42|P-value:1.81E-17||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:13.2|P-value:2.77E-40||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:4.61|P-value:1.99E-6||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VAM10(YOR068C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VPS1(YKR001C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS28(YPL065W)|FD-Score:4.92|P-value:4.35E-7||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:WTM2(YOR229W)|FD-Score:-4.15|P-value:1.65E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YBL062W(YBL062W_d)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL042W(YCL042W_p)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDL009C(YDL009C_p)|FD-Score:3.74|P-value:9.15E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:10.5|P-value:2.60E-26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR274C(YDR274C_d)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER097W(YER097W_d)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:6.67|P-value:1.26E-11||SGD DESC:Putative protein of unknown function Gene:YJL055W(YJL055W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKL136W(YKL136W_d)|FD-Score:-4.89|P-value:5.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YMR172C-A(YMR172C-A_d)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:-5.1|P-value:1.71E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR059C(YPR059C_d)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:AAC3(YBR085W)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:4.4|P-value:5.42E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ANT1(YPR128C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARO1(YDR127W)|FD-Score:5.21|P-value:9.58E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:AVO2(YMR068W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BRE1(YDL074C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BTS1(YPL069C)|FD-Score:-4.94|P-value:3.85E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CIN8(YEL061C)|FD-Score:4.41|P-value:5.12E-6||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COG6(YNL041C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX10(YPL172C)|FD-Score:6.99|P-value:1.36E-12||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:COX8(YLR395C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUR1(YPR158W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DCG1(YIR030C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DDR2(YOL052C-A)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DFG5(YMR238W)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DIN7(YDR263C)|FD-Score:-3.94|P-value:4.04E-5||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DRS2(YAL026C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSF2(YBR007C_p)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EFG1(YGR271C-A)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELA1(YNL230C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ERV14(YGL054C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EXO1(YOR033C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FBP1(YLR377C)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FCY2(YER056C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FPR2(YDR519W)|FD-Score:-3.75|P-value:8.92E-5||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GEP4(YHR100C)|FD-Score:4.86|P-value:5.75E-7||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIP3(YPL137C)|FD-Score:4.8|P-value:8.06E-7||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GPR1(YDL035C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GYP1(YOR070C)|FD-Score:6|P-value:9.71E-10||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOM2(YDR158W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSP42(YDR171W)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IMA1(YGR287C)|FD-Score:-5.7|P-value:6.05E-9||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:JNM1(YMR294W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KCS1(YDR017C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LNP1(YHR192W_p)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MAC1(YMR021C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDG1(YNL173C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MRPL6(YHR147C)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB4(YOL112W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MST1(YKL194C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MSY1(YPL097W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MTM1(YGR257C)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NAT5(YOR253W)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NOP12(YOL041C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NUP2(YLR335W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OM14(YBR230C)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OXR1(YPL196W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PCK1(YKR097W)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PEP8(YJL053W)|FD-Score:4.35|P-value:6.88E-6||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX28(YHR150W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:POP2(YNR052C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PST1(YDR055W)|FD-Score:-3.79|P-value:7.61E-5||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PUS7(YOR243C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RIM13(YMR154C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:6.02|P-value:8.69E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS23A(YGR118W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RTC3(YHR087W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SIZ1(YDR409W)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLO1(YER180C-A)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:STB2(YMR053C)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TKL1(YPR074C)|FD-Score:-4.28|P-value:9.30E-6||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:4.84|P-value:6.49E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:12.3|P-value:3.81E-35||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.42|P-value:1.81E-17||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:13.2|P-value:2.77E-40||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:4.61|P-value:1.99E-6||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VAM10(YOR068C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VPS1(YKR001C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS28(YPL065W)|FD-Score:4.92|P-value:4.35E-7||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:WTM2(YOR229W)|FD-Score:-4.15|P-value:1.65E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YBL062W(YBL062W_d)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL042W(YCL042W_p)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDL009C(YDL009C_p)|FD-Score:3.74|P-value:9.15E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:10.5|P-value:2.60E-26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR274C(YDR274C_d)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER097W(YER097W_d)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:6.67|P-value:1.26E-11||SGD DESC:Putative protein of unknown function Gene:YJL055W(YJL055W_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKL136W(YKL136W_d)|FD-Score:-4.89|P-value:5.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YMR172C-A(YMR172C-A_d)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:-5.1|P-value:1.71E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR059C(YPR059C_d)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL196W_d9.363.81E-211.56YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YMR203W7.803.02E-151.54TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YBR055C6.261.90E-100.55PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YBR192W5.725.44E-90.35RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YMR033W5.364.07E-80.04ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YFR037C5.325.14E-80.37RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YML091C4.963.60E-70.03RPM2Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
YGL047W4.934.13E-70.16ALG13Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
YGL093W4.779.43E-70.47SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YGL069C_d4.298.82E-60.11YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YIL126W4.181.44E-50.18STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YHR197W4.003.11E-50.02RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YGL055W3.993.34E-50.08OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YMR197C3.914.68E-50.01VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YCL043C3.894.96E-50.16PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR354W13.202.77E-40TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YER090W12.303.81E-35TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR008C_d10.502.60E-26YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL211C8.421.81E-17TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YPL172C6.991.36E-12COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YGL117W_p6.671.26E-11YGL117W_pPutative protein of unknown function
YDL020C6.028.69E-10RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YOR070C6.009.71E-10GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YDR127W5.219.58E-8ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YBR058C-A5.131.44E-7TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YOL052C-A4.953.63E-7DDR2Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
YPL065W4.924.35E-7VPS28Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
YHR100C4.865.75E-7GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YGL186C4.846.49E-7TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YPL137C4.808.06E-7GIP3Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1952
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3781.52E-199SGTC_2733amorolfine 100.0 μMMiscellaneous542600.106667plasma membrane duress
0.3276.86E-147SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.164384
0.3221.23E-142SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0789474
0.3182.27E-138SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.1
0.3113.70E-132SGTC_2725haloperidol 53.2 μMMiscellaneous35590.128205plasma membrane duress
0.3011.51E-123SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.107692
0.2958.57E-119SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0543478
0.2923.20E-116SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.0875TSC3-RPN4
0.2923.92E-116SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.0897436
0.2916.93E-116SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.189189plasma membrane duress
0.2911.09E-115SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0649351
0.2886.98E-113SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.27027
0.2851.88E-110SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.1TSC3-RPN4
0.2833.15E-109SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0789474
0.2838.77E-109SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0412371

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1957st07703562.4 μM0.78705593TimTec (Natural product derivative library)278.73413.19212
SGTC_1793st04825672.23 μM0.764706672022TimTec (Natural product derivative library)274.315022.51113
SGTC_1954st07703268.9 μM0.517857698907TimTec (Natural product derivative library)290.359023.68611
SGTC_2696st07769174.32 μM0.480769757133TimTec (Natural product derivative library)200.279542.83611
SGTC_800159-0065512.24 μM0.3768121548079ChemDiv (Drug-like library)390.431784.49814
SGTC_1636st00770768.9 μM0.370968403051TimTec (Natural product derivative library)290.359023.75911
SGTC_1885st05754699.9 μM0.368421702008TimTec (Natural product derivative library)200.236482.18321
SGTC_13943474-000165.7 μM0.3606565017666ChemDiv (Drug-like library)229.340642.46520endomembrane recycling
SGTC_2414st07723292.9 μM0.350877729801TimTec (Natural product derivative library)215.247823.13622
SGTC_13291447-169750.6 μM0.3428572840515ChemDiv (Drug-like library)286.325722.57623