st077032

(4-methylphenyl)-(1,3,4,5-tetrahydropyrido[4,3-b]indol-2-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1954
Screen concentration 68.9 μM
Source TimTec (Natural product derivative library)
PubChem CID 698907
SMILES CC1=CC=C(C=C1)C(=O)N2CCC3=C(C2)C4=CC=CC=C4N3
Standardized SMILES Cc1ccc(cc1)C(=O)N2CCc3[nH]c4ccccc4c3C2
Molecular weight 290.359
ALogP 3.69
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.78
% growth inhibition (Hom. pool) 1.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 698907
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:3.15|P-value:8.16E-4|Clearance:0.14||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CSE1(YGL238W)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.04||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:CUS1(YMR240C)|FD-Score:4.32|P-value:7.83E-6|Clearance:0.12||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:MAK5(YBR142W)|FD-Score:-5.94|P-value:1.39E-9|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MCD1(YDL003W)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.06||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MCD4(YKL165C)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.11||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MVD1(YNR043W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.31||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:POL12(YBL035C)|FD-Score:-3.77|P-value:8.12E-5|Clearance:0||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:POP1(YNL221C)|FD-Score:5.71|P-value:5.80E-9|Clearance:1.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP43(YGL120C)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.01||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RKI1(YOR095C)|FD-Score:-5.67|P-value:7.06E-9|Clearance:0||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RPB11(YOL005C)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPC82(YPR190C)|FD-Score:-4.17|P-value:1.50E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPS3(YNL178W)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SEC11(YIR022W)|FD-Score:4.06|P-value:2.41E-5|Clearance:0.44||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:STU2(YLR045C)|FD-Score:4.53|P-value:2.91E-6|Clearance:0.21||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:TLG1(YDR468C)|FD-Score:4.63|P-value:1.87E-6|Clearance:0.09||SGD DESC:Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p Gene:UTP20(YBL004W)|FD-Score:-5.04|P-value:2.33E-7|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YKL111C(YKL111C_d)|FD-Score:-3.19|P-value:7.23E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:CCT3(YJL014W)|FD-Score:3.15|P-value:8.16E-4|Clearance:0.14||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CSE1(YGL238W)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.04||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:CUS1(YMR240C)|FD-Score:4.32|P-value:7.83E-6|Clearance:0.12||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:MAK5(YBR142W)|FD-Score:-5.94|P-value:1.39E-9|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MCD1(YDL003W)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.06||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MCD4(YKL165C)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.11||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MVD1(YNR043W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.31||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:POL12(YBL035C)|FD-Score:-3.77|P-value:8.12E-5|Clearance:0||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:POP1(YNL221C)|FD-Score:5.71|P-value:5.80E-9|Clearance:1.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP43(YGL120C)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.01||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RKI1(YOR095C)|FD-Score:-5.67|P-value:7.06E-9|Clearance:0||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RPB11(YOL005C)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPC82(YPR190C)|FD-Score:-4.17|P-value:1.50E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPS3(YNL178W)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SEC11(YIR022W)|FD-Score:4.06|P-value:2.41E-5|Clearance:0.44||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:STU2(YLR045C)|FD-Score:4.53|P-value:2.91E-6|Clearance:0.21||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:TLG1(YDR468C)|FD-Score:4.63|P-value:1.87E-6|Clearance:0.09||SGD DESC:Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p Gene:UTP20(YBL004W)|FD-Score:-5.04|P-value:2.33E-7|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YKL111C(YKL111C_d)|FD-Score:-3.19|P-value:7.23E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 698907
Download HOP data (tab-delimited text)  (excel)
Gene:AFG3(YER017C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AFT1(YGL071W)|FD-Score:4.96|P-value:3.57E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGE2(YIL044C)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AHA1(YDR214W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:APP1(YNL094W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARL1(YBR164C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO2(YGL148W)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATX2(YOR079C)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation Gene:BSP1(YPR171W)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CTA1(YDR256C)|FD-Score:-4.69|P-value:1.35E-6||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CUE5(YOR042W)|FD-Score:-3.74|P-value:9.16E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUP9(YPL177C)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DRS2(YAL026C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ENT1(YDL161W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERD1(YDR414C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ESC1(YMR219W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FRK1(YPL141C)|FD-Score:3.79|P-value:7.47E-5||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FZF1(YGL254W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GLE2(YER107C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HCR1(YLR192C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HNT3(YOR258W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HSP42(YDR171W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:HTL1(YCR020W-B)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:INP2(YMR163C)|FD-Score:4.76|P-value:9.70E-7||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:KAP114(YGL241W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KRE2(YDR483W)|FD-Score:-3.81|P-value:6.98E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:MDM31(YHR194W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MED1(YPR070W)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MHF2(YDL160C-A)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MNL2(YLR057W)|FD-Score:4.97|P-value:3.42E-7||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MOH1(YBL049W)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MPC2(YHR162W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRE11(YMR224C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRK1(YDL079C)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MSL1(YIR009W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:MXR1(YER042W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAP1(YKR048C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:OAC1(YKL120W)|FD-Score:-6.18|P-value:3.28E-10||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OPI3(YJR073C)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSW1(YOR255W)|FD-Score:-3.87|P-value:5.53E-5||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:OXA1(YER154W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PAD1(YDR538W)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PEP7(YDR323C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET54(YGR222W)|FD-Score:-4.55|P-value:2.73E-6||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:RPL16A(YIL133C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL24B(YGR148C)|FD-Score:-5.26|P-value:7.05E-8||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:5.26|P-value:7.25E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:RRT2(YBR246W)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SAP4(YGL229C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEG1(YMR086W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SLT2(YHR030C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOD1(YJR104C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPC2(YML055W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SSA4(YER103W)|FD-Score:6.18|P-value:3.21E-10||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SVL3(YPL032C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TOS3(YGL179C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:TPN1(YGL186C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM2(YKR056W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRP2(YER090W)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:5.81|P-value:3.13E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA2(YBR006W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VEL1(YGL258W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS29(YHR012W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS30(YPL120W)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS36(YLR417W)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:5.29|P-value:6.01E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR012C(YBR012C_d)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR178W(YBR178W_d)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCF1(YDR135C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDR391C(YDR391C_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YER175W-A(YER175W-A_p)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YFL012W(YFL012W_p)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL260W(YGL260W_p)|FD-Score:-3.25|P-value:5.77E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR042W(YGR042W_p)|FD-Score:-3.83|P-value:6.54E-5||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YGR107W(YGR107W_d)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR266W(YGR266W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YJL175W(YJL175W_d)|FD-Score:-3.94|P-value:4.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YJL215C(YJL215C_d)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL070W(YKL070W_p)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL169C(YKL169C_d)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKR011C(YKR011C_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR001C(YLR001C_p)|FD-Score:4.37|P-value:6.12E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR225C(YLR225C_p)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YMR178W(YMR178W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR244W(YMR244W_p)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Putative protein of unknown function Gene:YNR040W(YNR040W_p)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOP1(YPR028W)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR277C(YOR277C_d)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL039W(YPL039W_p)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPQ1(YOL092W_p)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR012W(YPR012W_d)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:AFG3(YER017C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AFT1(YGL071W)|FD-Score:4.96|P-value:3.57E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGE2(YIL044C)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AHA1(YDR214W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:APP1(YNL094W)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARL1(YBR164C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO2(YGL148W)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATX2(YOR079C)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation Gene:BSP1(YPR171W)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CTA1(YDR256C)|FD-Score:-4.69|P-value:1.35E-6||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CUE5(YOR042W)|FD-Score:-3.74|P-value:9.16E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUP9(YPL177C)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DRS2(YAL026C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ENT1(YDL161W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERD1(YDR414C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ESC1(YMR219W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FRK1(YPL141C)|FD-Score:3.79|P-value:7.47E-5||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FZF1(YGL254W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GLE2(YER107C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HCR1(YLR192C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HNT3(YOR258W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HSP42(YDR171W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:HTL1(YCR020W-B)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:INP2(YMR163C)|FD-Score:4.76|P-value:9.70E-7||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:KAP114(YGL241W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KRE2(YDR483W)|FD-Score:-3.81|P-value:6.98E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:MDM31(YHR194W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MED1(YPR070W)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MHF2(YDL160C-A)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MNL2(YLR057W)|FD-Score:4.97|P-value:3.42E-7||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MOH1(YBL049W)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MPC2(YHR162W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRE11(YMR224C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRK1(YDL079C)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MSL1(YIR009W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:MXR1(YER042W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAP1(YKR048C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:OAC1(YKL120W)|FD-Score:-6.18|P-value:3.28E-10||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OPI3(YJR073C)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSW1(YOR255W)|FD-Score:-3.87|P-value:5.53E-5||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:OXA1(YER154W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PAD1(YDR538W)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PEP7(YDR323C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET54(YGR222W)|FD-Score:-4.55|P-value:2.73E-6||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:RPL16A(YIL133C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL24B(YGR148C)|FD-Score:-5.26|P-value:7.05E-8||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:5.26|P-value:7.25E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:RRT2(YBR246W)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SAP4(YGL229C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEG1(YMR086W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SLT2(YHR030C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOD1(YJR104C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPC2(YML055W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SSA4(YER103W)|FD-Score:6.18|P-value:3.21E-10||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SVL3(YPL032C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TOS3(YGL179C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:TPN1(YGL186C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM2(YKR056W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRP2(YER090W)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:5.81|P-value:3.13E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA2(YBR006W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VEL1(YGL258W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS29(YHR012W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS30(YPL120W)|FD-Score:4.49|P-value:3.57E-6||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS36(YLR417W)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:5.29|P-value:6.01E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR012C(YBR012C_d)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR178W(YBR178W_d)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCF1(YDR135C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDR391C(YDR391C_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YER175W-A(YER175W-A_p)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YFL012W(YFL012W_p)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL260W(YGL260W_p)|FD-Score:-3.25|P-value:5.77E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR042W(YGR042W_p)|FD-Score:-3.83|P-value:6.54E-5||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YGR107W(YGR107W_d)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR266W(YGR266W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YJL175W(YJL175W_d)|FD-Score:-3.94|P-value:4.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YJL215C(YJL215C_d)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL070W(YKL070W_p)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL169C(YKL169C_d)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKR011C(YKR011C_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR001C(YLR001C_p)|FD-Score:4.37|P-value:6.12E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR225C(YLR225C_p)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YMR178W(YMR178W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR244W(YMR244W_p)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Putative protein of unknown function Gene:YNR040W(YNR040W_p)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOP1(YPR028W)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR277C(YOR277C_d)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL039W(YPL039W_p)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPQ1(YOL092W_p)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR012W(YPR012W_d)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL221C5.715.80E-91.08POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YDR468C4.631.87E-60.09TLG1Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
YLR045C4.532.91E-60.21STU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
YMR240C4.327.83E-60.12CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YNL178W4.201.36E-50.08RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YDL003W4.121.89E-50.06MCD1Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
YIR022W4.062.41E-50.44SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YNR043W3.621.47E-40.31MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YKL165C3.314.71E-40.11MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YGL238W3.206.92E-40.04CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YGL120C3.167.86E-40.01PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YJL014W3.158.16E-40.14CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YKL125W3.010.001310.12RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YHR036W2.890.001910.20BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YDL145C2.690.003580.02COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER103W6.183.21E-10SSA4Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
YBR058C-A5.813.13E-9TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR136C_d5.296.01E-8VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YDL020C5.267.25E-8RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLR057W4.973.42E-7MNL2Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
YGL071W4.963.57E-7AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YMR163C4.769.70E-7INP2Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene
YML055W4.741.09E-6SPC2Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25
YDR538W4.592.18E-6PAD1Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX
YMR224C4.582.33E-6MRE11Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
YPL120W4.493.57E-6VPS30Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
YLR001C_p4.376.12E-6YLR001C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
YPR028W4.327.87E-6YOP1Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress
YJR104C4.299.00E-6SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YKL070W_p4.201.33E-5YKL070W_pPutative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_1954
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1561.27E-33SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.121212TSC3-RPN4
0.1562.10E-33SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.129032
0.1541.23E-32SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.15625TSC3-RPN4
0.1512.58E-31SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.123288
0.1461.36E-29SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0735294TSC3-RPN4
0.1455.14E-29SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.132353TSC3-RPN4
0.1411.95E-27SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.155844TSC3-RPN4
0.1367.73E-26SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.136364TSC3-RPN4
0.1343.62E-25SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0434783TSC3-RPN4
0.1322.38E-24SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.129032
0.1287.17E-23SGTC_8600443-0016 25.1 μMChemDiv (Drug-like library)53379420.125
0.1287.56E-23SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.151515
0.1256.51E-22SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.111111TSC3-RPN4
0.1232.24E-21SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0625
0.1233.33E-21SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0677966

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1636st00770768.9 μM0.576923403051TimTec (Natural product derivative library)290.359023.75911
SGTC_1952st07703664.1 μM0.5178572937007TimTec (Natural product derivative library)258.315623.01412
SGTC_1957st07703562.4 μM0.433333705593TimTec (Natural product derivative library)278.73413.19212
SGTC_1793st04825672.23 μM0.42623672022TimTec (Natural product derivative library)274.315022.51113
SGTC_2696st07769174.32 μM0.37037757133TimTec (Natural product derivative library)200.279542.83611
SGTC_2414st07723292.9 μM0.345455729801TimTec (Natural product derivative library)215.247823.13622
SGTC_3158910000749.47 μM0.30645219297233Chembridge (Drug-like library)373.286844.01902
SGTC_1825st05214362.6 μM0.305085674173TimTec (Natural product derivative library)319.44334.22412
SGTC_1953st07680012.1 μM0.301887297591TimTec (Natural product derivative library)219.281124.53410
SGTC_20155119600184 μM0.2982462828786Chembridge (Fragment library)200.236482.07521