wogonin

5,7-dihydroxy-8-methoxy-2-phenylchromen-4-one

A flavanone.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1955
Screen concentration 32.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 5281703
SMILES COC1=C(C=C(C2=C1OC(=CC2=O)C3=CC=CC=C3)O)O
Standardized SMILES COc1c(O)cc(O)c2C(=O)C=C(Oc12)c3ccccc3
Molecular weight 284.2635
ALogP 2.64
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.35
% growth inhibition (Hom. pool) 8.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5281703
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.8|P-value:7.18E-5|Clearance:0.05||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP9(YMR033W)|FD-Score:-4.17|P-value:1.52E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT3(YJL014W)|FD-Score:-3.19|P-value:7.18E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC31(YOR257W)|FD-Score:-3.22|P-value:6.43E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:JIP5(YPR169W)|FD-Score:4.09|P-value:2.20E-5|Clearance:0.2||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:NMD3(YHR170W)|FD-Score:-3.1|P-value:9.59E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG1(YPL093W)|FD-Score:-3.19|P-value:7.15E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOG2(YNR053C)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:PIS1(YPR113W)|FD-Score:3.75|P-value:8.83E-5|Clearance:0.06||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:QRI1(YDL103C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.06||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RPC19(YNL113W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.32||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:SEC21(YNL287W)|FD-Score:3.16|P-value:7.91E-4|Clearance:0.01||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC31(YDL195W)|FD-Score:9.64|P-value:2.70E-22|Clearance:5.55||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SGV1(YPR161C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.02||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SNP1(YIL061C)|FD-Score:-3.1|P-value:9.75E-4|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:3.88|P-value:5.18E-5|Clearance:0.08||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TFA1(YKL028W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.19||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TOM40(YMR203W)|FD-Score:-3.83|P-value:6.34E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP20(YBL004W)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:-4.77|P-value:9.38E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ACT1(YFL039C)|FD-Score:3.8|P-value:7.18E-5|Clearance:0.05||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP9(YMR033W)|FD-Score:-4.17|P-value:1.52E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT3(YJL014W)|FD-Score:-3.19|P-value:7.18E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC31(YOR257W)|FD-Score:-3.22|P-value:6.43E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:JIP5(YPR169W)|FD-Score:4.09|P-value:2.20E-5|Clearance:0.2||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:NMD3(YHR170W)|FD-Score:-3.1|P-value:9.59E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG1(YPL093W)|FD-Score:-3.19|P-value:7.15E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOG2(YNR053C)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:PIS1(YPR113W)|FD-Score:3.75|P-value:8.83E-5|Clearance:0.06||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:QRI1(YDL103C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.06||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RPC19(YNL113W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.32||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:SEC21(YNL287W)|FD-Score:3.16|P-value:7.91E-4|Clearance:0.01||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC31(YDL195W)|FD-Score:9.64|P-value:2.70E-22|Clearance:5.55||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SGV1(YPR161C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.02||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SNP1(YIL061C)|FD-Score:-3.1|P-value:9.75E-4|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:3.88|P-value:5.18E-5|Clearance:0.08||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TFA1(YKL028W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.19||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TOM40(YMR203W)|FD-Score:-3.83|P-value:6.34E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP20(YBL004W)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:-4.77|P-value:9.38E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5281703
Download HOP data (tab-delimited text)  (excel)
Gene:ALG12(YNR030W)|FD-Score:6.22|P-value:2.52E-10||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ALG6(YOR002W)|FD-Score:-3.74|P-value:9.30E-5||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARL1(YBR164C)|FD-Score:-5.99|P-value:1.07E-9||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO4(YBR249C)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:BNA3(YJL060W)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BSC2(YDR275W)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BZZ1(YHR114W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CAF120(YNL278W)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CTA1(YDR256C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CUL3(YGR003W)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:CYS4(YGR155W)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:4.49|P-value:3.55E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DOG2(YHR043C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DSS1(YMR287C)|FD-Score:5.68|P-value:6.63E-9||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DUR3(YHL016C)|FD-Score:-4.12|P-value:1.85E-5||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ERG3(YLR056W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FIN1(YDR130C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FZF1(YGL254W)|FD-Score:5.22|P-value:9.17E-8||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GLO1(YML004C)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GLO2(YDR272W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:HEF3(YNL014W)|FD-Score:-4.41|P-value:5.25E-6||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:ILV6(YCL009C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:INO2(YDR123C)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:IRC10(YOL015W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:MAC1(YMR021C)|FD-Score:-5.83|P-value:2.82E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MEP2(YNL142W)|FD-Score:-3.91|P-value:4.54E-5||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MNN11(YJL183W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MON1(YGL124C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL11(YDL202W)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS35(YGR165W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSC7(YHR039C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSN4(YKL062W)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MUB1(YMR100W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NHP6A(YPR052C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:PGD1(YGL025C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PSY2(YNL201C)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:REV7(YIL139C)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RFU1(YLR073C)|FD-Score:-4.75|P-value:1.01E-6||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL24B(YGR148C)|FD-Score:-3.77|P-value:8.03E-5||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SCO1(YBR037C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SCP160(YJL080C)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SEE1(YIL064W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SFB3(YHR098C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SLX1(YBR228W)|FD-Score:6.18|P-value:3.17E-10||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SRX1(YKL086W)|FD-Score:-3.89|P-value:4.99E-5||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SSD1(YDR293C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYS1(YJL004C)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA8(YAL064C-A_p)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TOM6(YOR045W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPK2(YPL203W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRP2(YER090W)|FD-Score:7.38|P-value:8.12E-14||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.95|P-value:1.85E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VNX1(YNL321W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS64(YDR200C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YAL037C-A(YAL037C-A_p)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Putative protein of unknown function Gene:YBR241C(YBR241C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:5.32|P-value:5.31E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGL242C(YGL242C_p)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR290W(YGR290W_d)|FD-Score:5.41|P-value:3.09E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YJL211C(YJL211C_d)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YJR111C(YJR111C_p)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YKL044W(YKL044W_p)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLL020C(YLL020C_d)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLL054C(YLL054C_p)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR225C(YLR225C_p)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR434C(YLR434C_d)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YLR455W(YLR455W_p)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR122C(YMR122C_d)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL140C(YNL140C_p)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOR114W(YOR114W_p)|FD-Score:-3.74|P-value:9.02E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL025C(YPL025C_d)|FD-Score:-3.73|P-value:9.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:-4.37|P-value:6.20E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL119C-A(YPL119C-A_p)|FD-Score:-3.16|P-value:7.82E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL197C(YPL197C_d)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:ALG12(YNR030W)|FD-Score:6.22|P-value:2.52E-10||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ALG6(YOR002W)|FD-Score:-3.74|P-value:9.30E-5||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARL1(YBR164C)|FD-Score:-5.99|P-value:1.07E-9||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO4(YBR249C)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:BNA3(YJL060W)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BSC2(YDR275W)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BZZ1(YHR114W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CAF120(YNL278W)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CTA1(YDR256C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CUL3(YGR003W)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:CYS4(YGR155W)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:4.49|P-value:3.55E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DOG2(YHR043C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DSS1(YMR287C)|FD-Score:5.68|P-value:6.63E-9||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DUR3(YHL016C)|FD-Score:-4.12|P-value:1.85E-5||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ERG3(YLR056W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FIN1(YDR130C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FZF1(YGL254W)|FD-Score:5.22|P-value:9.17E-8||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GLO1(YML004C)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GLO2(YDR272W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:HEF3(YNL014W)|FD-Score:-4.41|P-value:5.25E-6||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:ILV6(YCL009C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:INO2(YDR123C)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:IRC10(YOL015W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:MAC1(YMR021C)|FD-Score:-5.83|P-value:2.82E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MEP2(YNL142W)|FD-Score:-3.91|P-value:4.54E-5||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MNN11(YJL183W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MON1(YGL124C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL11(YDL202W)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS35(YGR165W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSC7(YHR039C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSN4(YKL062W)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MUB1(YMR100W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NHP6A(YPR052C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:PGD1(YGL025C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PSY2(YNL201C)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:REV7(YIL139C)|FD-Score:4.35|P-value:6.86E-6||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RFU1(YLR073C)|FD-Score:-4.75|P-value:1.01E-6||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL24B(YGR148C)|FD-Score:-3.77|P-value:8.03E-5||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SCO1(YBR037C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SCP160(YJL080C)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SEE1(YIL064W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SFB3(YHR098C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SLX1(YBR228W)|FD-Score:6.18|P-value:3.17E-10||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SRX1(YKL086W)|FD-Score:-3.89|P-value:4.99E-5||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SSD1(YDR293C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYS1(YJL004C)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA8(YAL064C-A_p)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TOM6(YOR045W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPK2(YPL203W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRP2(YER090W)|FD-Score:7.38|P-value:8.12E-14||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.95|P-value:1.85E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VNX1(YNL321W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS64(YDR200C)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YAL037C-A(YAL037C-A_p)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Putative protein of unknown function Gene:YBR241C(YBR241C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:5.32|P-value:5.31E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGL242C(YGL242C_p)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR290W(YGR290W_d)|FD-Score:5.41|P-value:3.09E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YJL211C(YJL211C_d)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YJR111C(YJR111C_p)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YKL044W(YKL044W_p)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLL020C(YLL020C_d)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLL054C(YLL054C_p)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR225C(YLR225C_p)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR434C(YLR434C_d)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YLR455W(YLR455W_p)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR122C(YMR122C_d)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL140C(YNL140C_p)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOR114W(YOR114W_p)|FD-Score:-3.74|P-value:9.02E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL025C(YPL025C_d)|FD-Score:-3.73|P-value:9.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:-4.37|P-value:6.20E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL119C-A(YPL119C-A_p)|FD-Score:-3.16|P-value:7.82E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL197C(YPL197C_d)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL195W9.642.70E-225.55SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPR169W4.092.20E-50.20JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YPL237W3.885.18E-50.08SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YFL039C3.807.18E-50.05ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YPR113W3.758.83E-50.06PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YPR161C3.691.13E-40.02SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YNL113W3.661.24E-40.32RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YKL028W3.354.09E-40.19TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YNL287W3.167.91E-40.01SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YDL103C3.148.32E-40.06QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YMR128W3.090.001010.03ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YLR378C3.060.001100.15SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YMR239C2.910.001820.01RNT1Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
YLR078C2.900.001860.04BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
YDL193W2.860.002130.03NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER090W7.388.12E-14TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR354W6.951.85E-12TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YNR030W6.222.52E-10ALG12Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation
YBR228W6.183.17E-10SLX1Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p
YMR287C5.686.63E-9DSS13'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
YGR290W_d5.413.09E-8YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YDR008C_d5.325.31E-8YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL254W5.229.17E-8FZF1Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress
YGR155W5.151.30E-7CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YKL211C4.572.44E-6TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YIR023W4.493.55E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YBR249C4.473.97E-6ARO43-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
YIL139C4.356.86E-6REV7Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
YDL216C4.279.97E-6RRI1Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling
YML124C4.251.06E-5TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1955
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2329.24E-73SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0340909
0.2228.54E-67SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.307692
0.2201.53E-65SGTC_1104tacrolimus 29.8 μMNIH Clinical Collection235817960.075
0.2181.16E-64SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.121212
0.2112.31E-60SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.016129
0.2074.90E-58SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0735294
0.2062.15E-57SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.0821918
0.1981.91E-53SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0555556
0.1966.88E-52SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0526316
0.1945.13E-51SGTC_22967971656 126.5 μMChembridge (Fragment library)6665700.109589
0.1939.10E-51SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.112676
0.1927.58E-50SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.12987
0.1857.97E-47SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.0897436
0.1842.41E-46SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0864198
0.1842.72E-46SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0857143

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1959st07709155.8 μM0.704545821356TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_1852st05625431.4 μM0.5625442583TimTec (Natural product derivative library)312.316643.08705
SGTC_1834st05598152.8 μM0.55102471719TimTec (Natural product derivative library)284.263482.63625RPP1 & pyrimidine depletion
SGTC_2515chrysin20.77 μM0.5434785281607Microsource (Natural product library)254.23752.65224RPP1 & pyrimidine depletion
SGTC_2600tangeritin87.95 μM0.53191568077Microsource (Natural product library)372.36863.05407
SGTC_1965Pectolinarigenin58.4 μM0.5294125320438TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_2513baicalein25.65 μM0.5106385281605Microsource (Natural product library)270.23692.4135calcium & mitochondrial duress
SGTC_15615-methoxyflavone52.9 μM0.594525TimTec (Pure natural product library)252.264683.1203
SGTC_2550chrysin dimethyl ether71.87 μM0.588881Microsource (Natural product library)282.290663.10304
SGTC_2593nobiletin86.24 μM0.572344Microsource (Natural product library)402.394583.03808