k015-0023

5-bromo-1-[(4-nitrophenyl)methyl]indole-2,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_196
Screen concentration 18.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 5211998
SMILES C1=CC(=CC=C1CN2C3=C(C=C(C=C3)Br)C(=O)C2=O)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(CN2C(=O)C(=O)c3cc(Br)ccc23)cc1
Molecular weight 361.147
ALogP 3.1
H-bond donor count 0
H-bond acceptor count 4
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.5
% growth inhibition (Hom. pool) 7.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5211998
Download HIP data (tab-delimited text)  (excel)
Gene:ERG26(YGL001C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.28||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FAS1(YKL182W)|FD-Score:-3.47|P-value:2.60E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GUS1(YGL245W)|FD-Score:-3.23|P-value:6.13E-4|Clearance:0||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:KRI1(YNL308C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.08||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MPE1(YKL059C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.07||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOP9(YJL010C)|FD-Score:4.1|P-value:2.06E-5|Clearance:0.38||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NTF2(YER009W)|FD-Score:-3.36|P-value:3.92E-4|Clearance:0||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:RVB1(YDR190C)|FD-Score:-3.56|P-value:1.87E-4|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:UTP8(YGR128C)|FD-Score:-3.35|P-value:3.99E-4|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YCG1(YDR325W)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.26||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:ERG26(YGL001C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.28||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FAS1(YKL182W)|FD-Score:-3.47|P-value:2.60E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GUS1(YGL245W)|FD-Score:-3.23|P-value:6.13E-4|Clearance:0||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:KRI1(YNL308C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.08||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MPE1(YKL059C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.07||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOP9(YJL010C)|FD-Score:4.1|P-value:2.06E-5|Clearance:0.38||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NTF2(YER009W)|FD-Score:-3.36|P-value:3.92E-4|Clearance:0||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:RVB1(YDR190C)|FD-Score:-3.56|P-value:1.87E-4|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:UTP8(YGR128C)|FD-Score:-3.35|P-value:3.99E-4|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YCG1(YDR325W)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.26||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5211998
Download HOP data (tab-delimited text)  (excel)
Gene:ACO2(YJL200C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:AKR2(YOR034C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:ALP1(YNL270C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:AUS1(YOR011W)|FD-Score:6.41|P-value:7.19E-11||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BBC1(YJL020C)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BSC2(YDR275W)|FD-Score:4.86|P-value:5.74E-7||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:CIN5(YOR028C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CTR9(YOL145C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DLD2(YDL178W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DST1(YGL043W)|FD-Score:4.29|P-value:8.89E-6||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECL1(YGR146C)|FD-Score:4.81|P-value:7.44E-7||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EMC2(YJR088C)|FD-Score:5.94|P-value:1.39E-9||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:ERG2(YMR202W)|FD-Score:5.66|P-value:7.55E-9||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FEN1(YCR034W)|FD-Score:5.95|P-value:1.36E-9||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FLO1(YAR050W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:GUD1(YDL238C)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HAC1(YFL031W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HVG1(YER039C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IMP2(YMR035W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IRS4(YKR019C)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAF1(YDR005C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MGT1(YDL200C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MMT1(YMR177W)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MYO5(YMR109W)|FD-Score:-5.07|P-value:2.00E-7||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:OCA6(YDR067C_p)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PBP1(YGR178C)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PBP4(YDL053C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:10.8|P-value:2.96E-27||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PPH3(YDR075W)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:QCR10(YHR001W-A)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:QDR1(YIL120W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RIM15(YFL033C)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RPL14B(YHL001W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM28(YDR494W)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SAC7(YDR389W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAP4(YGL229C)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SBH2(YER019C-A)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SIW14(YNL032W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SNQ2(YDR011W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPS4(YOR313C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SPT3(YDR392W)|FD-Score:7.3|P-value:1.44E-13||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SWC5(YBR231C)|FD-Score:5.64|P-value:8.46E-9||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWT21(YNL187W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:SYF2(YGR129W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:THI4(YGR144W)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:VPH2(YKL119C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:YDL026W(YDL026W_d)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL183C(YDL183C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YEN1(YER041W)|FD-Score:3.74|P-value:9.20E-5||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER010C(YER010C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YER034W(YER034W_p)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGL108C(YGL108C_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGR126W(YGR126W_p)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR127W(YGR127W_p)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR130C(YGR130C)|FD-Score:5.85|P-value:2.43E-9||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YGR151C(YGR151C_d)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YLR374C(YLR374C_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YOL131W(YOL131W_p)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function Gene:YOR325W(YOR325W_d)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPK1(YKL126W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ACO2(YJL200C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:AKR2(YOR034C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:ALP1(YNL270C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:AUS1(YOR011W)|FD-Score:6.41|P-value:7.19E-11||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BBC1(YJL020C)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BSC2(YDR275W)|FD-Score:4.86|P-value:5.74E-7||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:CIN5(YOR028C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CTR9(YOL145C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DLD2(YDL178W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DST1(YGL043W)|FD-Score:4.29|P-value:8.89E-6||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECL1(YGR146C)|FD-Score:4.81|P-value:7.44E-7||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EMC2(YJR088C)|FD-Score:5.94|P-value:1.39E-9||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:ERG2(YMR202W)|FD-Score:5.66|P-value:7.55E-9||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FEN1(YCR034W)|FD-Score:5.95|P-value:1.36E-9||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FLO1(YAR050W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:GUD1(YDL238C)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HAC1(YFL031W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HVG1(YER039C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IMP2(YMR035W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IRS4(YKR019C)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAF1(YDR005C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MGT1(YDL200C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MMT1(YMR177W)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MYO5(YMR109W)|FD-Score:-5.07|P-value:2.00E-7||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:OCA6(YDR067C_p)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PBP1(YGR178C)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PBP4(YDL053C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:10.8|P-value:2.96E-27||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PPH3(YDR075W)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:QCR10(YHR001W-A)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:QDR1(YIL120W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RIM15(YFL033C)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RPL14B(YHL001W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM28(YDR494W)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SAC7(YDR389W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAP4(YGL229C)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SBH2(YER019C-A)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SIW14(YNL032W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SNQ2(YDR011W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPS4(YOR313C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SPT3(YDR392W)|FD-Score:7.3|P-value:1.44E-13||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SWC5(YBR231C)|FD-Score:5.64|P-value:8.46E-9||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWT21(YNL187W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:SYF2(YGR129W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:THI4(YGR144W)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:VPH2(YKL119C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:YDL026W(YDL026W_d)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL183C(YDL183C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YEN1(YER041W)|FD-Score:3.74|P-value:9.20E-5||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER010C(YER010C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YER034W(YER034W_p)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGL108C(YGL108C_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGR126W(YGR126W_p)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR127W(YGR127W_p)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR130C(YGR130C)|FD-Score:5.85|P-value:2.43E-9||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YGR151C(YGR151C_d)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YLR374C(YLR374C_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YOL131W(YOL131W_p)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function Gene:YOR325W(YOR325W_d)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPK1(YKL126W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL010C4.102.06E-50.38NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YDR325W3.721.00E-40.26YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YKL059C3.462.68E-40.07MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YGL001C3.393.45E-40.28ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YNL308C3.129.16E-40.08KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YDR468C3.040.001180.08TLG1Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
YDL221W_d2.960.001540.06YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YML010W2.900.001860.14SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YMR277W2.760.002870.06FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YDR145W2.700.003450.07TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YLR029C2.630.004260.12RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YMR168C2.510.006030.15CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YMR059W2.360.009170.02SEN15Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YLR076C_d2.340.009570.01YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YJL015C_d2.330.009810.00YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C10.802.96E-27PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YDR392W7.301.44E-13SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YOR011W6.417.19E-11AUS1Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen
YCR034W5.951.36E-9FEN1Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication
YJR088C5.941.39E-9EMC2Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
YGR130C5.852.43E-9YGR130CComponent of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7)
YMR202W5.667.55E-9ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YBR231C5.648.46E-9SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YER039C5.161.22E-7HVG1Protein of unknown function, has homology to Vrg4p
YDR275W4.865.74E-7BSC2Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication
YGR146C4.817.44E-7ECL1Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation
YER034W_p4.681.44E-6YER034W_pProtein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
YDL026W_d4.552.69E-6YDL026W_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
YGL043W4.298.89E-6DST1General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
YGR129W4.141.71E-5SYF2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest

GO enrichment analysis for SGTC_196
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2231.77E-67SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0779221ERG2
0.2104.14E-60SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0930233PDR1
0.2108.96E-60SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.108434ERG2
0.2101.28E-59SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.149254ERG2
0.2034.85E-56SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.695652PDR1
0.1971.23E-52SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.156627PDR1
0.1931.01E-50SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.11236PDR1
0.1783.94E-43SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.0985916sphingolipid biosynthesis & PDR1
0.1702.10E-39SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.132353
0.1652.72E-37SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0491803PDR1
0.1641.40E-36SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.357143PDR1
0.1599.57E-35SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.0519481ERG2
0.1553.12E-33SGTC_489paxilline 115.0 μMICCB bioactive library1050080.0526316PDR1
0.1549.74E-33SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0632911ERG2
0.1541.09E-32SGTC_29329016711 44.5 μMChembridge (Drug-like library)71173280.0657895

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1027k015-00258.67 μM0.760873598955ChemDiv (Drug-like library)361.146963.10204
SGTC_295k015-002731 μM0.6956523887424ChemDiv (Drug-like library)296.277482.8404PDR1
SGTC_169k015-001716.03 μM0.6818181840002ChemDiv (Drug-like library)334.1398633.41303
SGTC_1028k015-00294.8 μM0.6326534588309ChemDiv (Drug-like library)351.141023.68304
SGTC_1458k015-00346.33 μM0.6086963598957ChemDiv (Drug-like library)352.1303263.61904
SGTC_544k015-002052.4 μM0.5555563008304ChemDiv (Drug-like library)316.14943.20802
SGTC_349k015-001424 μM0.522196908ChemDiv (Drug-like library)350.594463.87202
SGTC_10483161-030024.5 μM0.5106382165101ChemDiv (Drug-like library)266.090722.24102
SGTC_1457k015-003257.3 μM0.54302660ChemDiv (Drug-like library)334.6864233.22405
SGTC_1031k035-000916 μM0.4333335180959ChemDiv (Drug-like library)378.174182.88505