st076550

2-[2-(4-methylphenyl)-4-oxochromen-3-yl]oxyacetonitrile

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1961
Screen concentration 68.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 1579337
SMILES CC1=CC=C(C=C1)C2=C(C(=O)C3=CC=CC=C3O2)OCC#N
Standardized SMILES Cc1ccc(cc1)C2=C(OCC#N)C(=O)c3ccccc3O2
Molecular weight 291.3007
ALogP 3.04
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.34
% growth inhibition (Hom. pool) 3.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1579337
Download HIP data (tab-delimited text)  (excel)
Gene:CDC31(YOR257W)|FD-Score:-5.29|P-value:6.11E-8|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC48(YDL126C)|FD-Score:4.18|P-value:1.49E-5|Clearance:0.39||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CRM1(YGR218W)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:DFR1(YOR236W)|FD-Score:3.17|P-value:7.59E-4|Clearance:0.19||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FOL2(YGR267C)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.16||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GPI18(YBR004C)|FD-Score:5.1|P-value:1.70E-7|Clearance:0.47||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:ILS1(YBL076C)|FD-Score:-4.31|P-value:8.26E-6|Clearance:0||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MSL5(YLR116W)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.15||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:PAP1(YKR002W)|FD-Score:-3.85|P-value:5.96E-5|Clearance:0||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PRP2(YNR011C)|FD-Score:6.28|P-value:1.67E-10|Clearance:0.98||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:PRP3(YDR473C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.04||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RIB2(YOL066C)|FD-Score:3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RLP7(YNL002C)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.04||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RVB2(YPL235W)|FD-Score:4.46|P-value:4.04E-6|Clearance:0.24||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:TFB1(YDR311W)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.08||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TRM5(YHR070W)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.06||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:UTP22(YGR090W)|FD-Score:3.79|P-value:7.68E-5|Clearance:0.07||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:UTP9(YHR196W)|FD-Score:6.08|P-value:5.89E-10|Clearance:0.98||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR190W(YBR190W_d)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL221W(YDL221W_d)|FD-Score:4.62|P-value:1.87E-6|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:YJL195C(YJL195C_d)|FD-Score:4.19|P-value:1.42E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:CDC31(YOR257W)|FD-Score:-5.29|P-value:6.11E-8|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC48(YDL126C)|FD-Score:4.18|P-value:1.49E-5|Clearance:0.39||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CRM1(YGR218W)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:DFR1(YOR236W)|FD-Score:3.17|P-value:7.59E-4|Clearance:0.19||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FOL2(YGR267C)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.16||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GPI18(YBR004C)|FD-Score:5.1|P-value:1.70E-7|Clearance:0.47||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:ILS1(YBL076C)|FD-Score:-4.31|P-value:8.26E-6|Clearance:0||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MSL5(YLR116W)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.15||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:PAP1(YKR002W)|FD-Score:-3.85|P-value:5.96E-5|Clearance:0||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PRP2(YNR011C)|FD-Score:6.28|P-value:1.67E-10|Clearance:0.98||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:PRP3(YDR473C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.04||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RIB2(YOL066C)|FD-Score:3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RLP7(YNL002C)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.04||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RVB2(YPL235W)|FD-Score:4.46|P-value:4.04E-6|Clearance:0.24||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:TFB1(YDR311W)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.08||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TRM5(YHR070W)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.06||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:UTP22(YGR090W)|FD-Score:3.79|P-value:7.68E-5|Clearance:0.07||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:UTP9(YHR196W)|FD-Score:6.08|P-value:5.89E-10|Clearance:0.98||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR190W(YBR190W_d)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL221W(YDL221W_d)|FD-Score:4.62|P-value:1.87E-6|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:YJL195C(YJL195C_d)|FD-Score:4.19|P-value:1.42E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1579337
Download HOP data (tab-delimited text)  (excel)
Gene:AIM20(YIL158W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:AIM37(YNL100W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIM45(YPR004C)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:AIM46(YHR199C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALE1(YOR175C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:BZZ1(YHR114W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CHD1(YER164W)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CTK3(YML112W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DIF1(YLR437C)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DMA1(YHR115C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS1(YMR287C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EAF6(YJR082C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ENT5(YDR153C)|FD-Score:-3.81|P-value:6.97E-5||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:ERI1(YPL096C-A)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FBP26(YJL155C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:HER2(YMR293C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HGH1(YGR187C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HRD3(YLR207W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:IMG1(YCR046C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:ISM1(YPL040C)|FD-Score:4.29|P-value:9.11E-6||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LDB7(YBL006C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MOH1(YBL049W)|FD-Score:7.34|P-value:1.03E-13||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MSC6(YOR354C)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MSG5(YNL053W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSN1(YOL116W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:PAD1(YDR538W)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PBS2(YJL128C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PEX19(YDL065C)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PEX27(YOR193W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:RAD18(YCR066W)|FD-Score:7.07|P-value:7.56E-13||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RPS11A(YDR025W)|FD-Score:-3.84|P-value:6.08E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTT107(YHR154W)|FD-Score:6.44|P-value:6.01E-11||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SFB3(YHR098C)|FD-Score:5.54|P-value:1.53E-8||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SMF1(YOL122C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins Gene:SMY1(YKL079W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SNF2(YOR290C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNZ2(YNL333W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SST2(YLR452C)|FD-Score:4|P-value:3.12E-5||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:TAT1(YBR069C)|FD-Score:5.75|P-value:4.47E-9||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:XKS1(YGR194C)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains Gene:YAP1(YML007W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR064W(YBR064W_d)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR139W(YBR139W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YGR273C(YGR273C_p)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YGR283C(YGR283C)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YHR078W(YHR078W_p)|FD-Score:4.87|P-value:5.61E-7||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YIL060W(YIL060W_p)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR374C(YLR374C_d)|FD-Score:5.26|P-value:7.38E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YNL276C(YNL276C_d)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOR1(YGR281W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPL014W(YPL014W_p)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:AIM20(YIL158W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:AIM37(YNL100W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIM45(YPR004C)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:AIM46(YHR199C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALE1(YOR175C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:BZZ1(YHR114W)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CHD1(YER164W)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CTK3(YML112W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DIF1(YLR437C)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DMA1(YHR115C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS1(YMR287C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EAF6(YJR082C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ENT5(YDR153C)|FD-Score:-3.81|P-value:6.97E-5||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:ERI1(YPL096C-A)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FBP26(YJL155C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:HER2(YMR293C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HGH1(YGR187C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HRD3(YLR207W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:IMG1(YCR046C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:ISM1(YPL040C)|FD-Score:4.29|P-value:9.11E-6||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LDB7(YBL006C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MOH1(YBL049W)|FD-Score:7.34|P-value:1.03E-13||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MSC6(YOR354C)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MSG5(YNL053W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSN1(YOL116W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:PAD1(YDR538W)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PBS2(YJL128C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PEX19(YDL065C)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PEX27(YOR193W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:RAD18(YCR066W)|FD-Score:7.07|P-value:7.56E-13||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RPS11A(YDR025W)|FD-Score:-3.84|P-value:6.08E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTT107(YHR154W)|FD-Score:6.44|P-value:6.01E-11||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SFB3(YHR098C)|FD-Score:5.54|P-value:1.53E-8||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SMF1(YOL122C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins Gene:SMY1(YKL079W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SNF2(YOR290C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNZ2(YNL333W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SST2(YLR452C)|FD-Score:4|P-value:3.12E-5||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:TAT1(YBR069C)|FD-Score:5.75|P-value:4.47E-9||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:XKS1(YGR194C)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains Gene:YAP1(YML007W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR064W(YBR064W_d)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR139W(YBR139W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YGR273C(YGR273C_p)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YGR283C(YGR283C)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YHR078W(YHR078W_p)|FD-Score:4.87|P-value:5.61E-7||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YIL060W(YIL060W_p)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR374C(YLR374C_d)|FD-Score:5.26|P-value:7.38E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YNL276C(YNL276C_d)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOR1(YGR281W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPL014W(YPL014W_p)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNR011C6.281.67E-100.98PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YHR196W6.085.89E-100.98UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YBR004C5.101.70E-70.47GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YDL221W_d4.621.87E-60.16YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YPL235W4.464.04E-60.24RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YNL002C4.221.20E-50.04RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YJL195C_d4.191.42E-50.01YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YDL126C4.181.49E-50.39CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YGR090W3.797.68E-50.07UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YGR267C3.711.02E-40.16FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YBR190W_d3.551.92E-40.06YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YDR473C3.492.39E-40.04PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YLR116W3.462.73E-40.14MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YDR311W3.314.63E-40.08TFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
YOL066C3.236.22E-40.00RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL049W7.341.03E-13MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YCR066W7.077.56E-13RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YHR154W6.446.01E-11RTT107Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
YBR069C5.754.47E-9TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YHR098C5.541.53E-8SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YLR374C_d5.267.38E-8YLR374C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W
YHR078W_p4.875.61E-7YHR078W_pHigh osmolarity-regulated gene of unknown function
YGR283C4.523.04E-6YGR283CPutative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus
YPL040C4.299.11E-6ISM1Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
YOL122C4.261.02E-5SMF1Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
YIL060W_p4.032.74E-5YIL060W_pPutative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
YLR452C4.003.12E-5SST2GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
YHR199C3.816.92E-5AIM46Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YLR437C3.758.80E-5DIF1Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein
YBR048W3.739.45E-5RPS11BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1961
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0822.37E-10SGTC_9831491-0821 12.2 μMChemDiv (Drug-like library)464951130.0540541
0.0809.00E-10SGTC_2779methotrexate 400.0 μMMiscellaneous1269410.0638298
0.0791.08E-9SGTC_1969st069479 40.1 μMTimTec (Natural product derivative library)38954850.0679612
0.0772.79E-9SGTC_26344'-methoxyflavone 100.0 μMMicrosource (Natural product library)777930.321429
0.0758.62E-9SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.0857143
0.0759.19E-9SGTC_2656pseudo-anisatin 100.0 μMMicrosource (Natural product library)67088090.0675676
0.0722.62E-8SGTC_7924534-1957 155.0 μMChemDiv (Drug-like library)17179040.125
0.0691.19E-7SGTC_8380109-0203 71.4 μMChemDiv (Drug-like library)7594220.166667
0.0691.33E-7SGTC_1963st076565 50.3 μMTimTec (Natural product derivative library)49142800.129412
0.0681.55E-7SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.0786517translation
0.0681.87E-7SGTC_1025k007-0731 70.0 μMChemDiv (Drug-like library)62561180.0735294
0.0682.07E-7SGTC_1618st003135 44.5 μMTimTec (Natural product derivative library)12706150.0674157
0.0663.16E-7SGTC_31249127567 49.5 μMChembridge (Drug-like library)175920300.102564DNA damage response
0.0663.21E-7SGTC_25354',4'-dimethoxydalbergione 51.2 μMMicrosource (Natural product library)3641060.101449DNA damage response
0.0631.43E-6SGTC_20315180455 200.0 μMChembridge (Fragment library)299490.0392157

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1864st05764530.4 μM0.530612688666TimTec (Natural product derivative library)282.290662.80804amide catabolism
SGTC_1868st05764442.7 μM0.444444688665TimTec (Natural product derivative library)282.290662.80804
SGTC_15494'-methoxyflavonol74.6 μM0.43396297141TimTec (Pure natural product library)268.264082.58214
SGTC_18693',7'-dimethoxyflavone40.3 μM0.4688664TimTec (Natural product derivative library)282.290662.80804
SGTC_25623',7'-dimethoxyflavone64.92 μM0.4688664Microsource (Natural product library)282.290662.80804
SGTC_1693xanthone101.9 μM0.3720937020TimTec (Natural product derivative library)196.201422.96402DNA damage response
SGTC_2532xanthone78.9 μM0.3720937020Microsource (Natural product library)196.201422.96402
SGTC_1112tnp000691.17 μM0.37037145826TimTec (Natural product library)270.23692.11435
SGTC_11103-hydroxy-3'-methoxyflavone1.24 μM0.362069676296TimTec (Natural product library)268.264082.58214iron homeostasis
SGTC_1830st05598250.3 μM0.352941455313TimTec (Natural product derivative library)254.23752.35624
SGTC_1109tnp00067928.26 nM0.345455676295TimTec (Natural product library)254.23752.35624
SGTC_1865st05763952.2 μM0.344828688818TimTec (Natural product derivative library)312.316642.79105