st076914

3-[3-(3-chloroanilino)propanoyl]chromen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1964
Screen concentration 61.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 24208661
SMILES C1=CC=C2C(=C1)C=C(C(=O)O2)C(=O)CCNC3=CC(=CC=C3)Cl
Standardized SMILES Clc1cccc(NCCC(=O)C2=Cc3ccccc3OC2=O)c1
Molecular weight 327.7617
ALogP 3.83
H-bond donor count 1
H-bond acceptor count 4
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.32
% growth inhibition (Hom. pool) 3.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24208661
Download HIP data (tab-delimited text)  (excel)
Gene:BET1(YIL004C)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.07||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CDC13(YDL220C)|FD-Score:3.17|P-value:7.51E-4|Clearance:0.12||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:ECM16(YMR128W)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.01||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:ENP2(YGR145W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.03||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:HIP1(YGR191W)|FD-Score:3.76|P-value:8.63E-5|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:NAF1(YNL124W)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NTR2(YKR022C)|FD-Score:3.72|P-value:9.89E-5|Clearance:0.15||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:RNA15(YGL044C)|FD-Score:3.24|P-value:5.88E-4|Clearance:0.07||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB10(YOR210W)|FD-Score:3.75|P-value:8.74E-5|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB11(YOL005C)|FD-Score:3.31|P-value:4.59E-4|Clearance:0.02||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPP1(YHR062C)|FD-Score:10.2|P-value:1.03E-24|Clearance:4.55||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:3.27|P-value:5.36E-4|Clearance:0.03||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP4(YHR069C)|FD-Score:4.93|P-value:4.07E-7|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC10(YLR166C)|FD-Score:5.65|P-value:8.22E-9|Clearance:0.71||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SNU71(YGR013W)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.35||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:SSL1(YLR005W)|FD-Score:4.3|P-value:8.56E-6|Clearance:0.1||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SUI3(YPL237W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.14||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TAF11(YML015C)|FD-Score:3.85|P-value:5.95E-5|Clearance:0.09||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:UBC1(YDR177W)|FD-Score:4.89|P-value:5.06E-7|Clearance:0.59||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP25(YIL091C)|FD-Score:-3.87|P-value:5.43E-5|Clearance:0||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YGL069C(YGL069C_d)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.02||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:BET1(YIL004C)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.07||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CDC13(YDL220C)|FD-Score:3.17|P-value:7.51E-4|Clearance:0.12||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:ECM16(YMR128W)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.01||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:ENP2(YGR145W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.03||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:HIP1(YGR191W)|FD-Score:3.76|P-value:8.63E-5|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:NAF1(YNL124W)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NTR2(YKR022C)|FD-Score:3.72|P-value:9.89E-5|Clearance:0.15||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:RNA15(YGL044C)|FD-Score:3.24|P-value:5.88E-4|Clearance:0.07||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB10(YOR210W)|FD-Score:3.75|P-value:8.74E-5|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB11(YOL005C)|FD-Score:3.31|P-value:4.59E-4|Clearance:0.02||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPP1(YHR062C)|FD-Score:10.2|P-value:1.03E-24|Clearance:4.55||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:3.27|P-value:5.36E-4|Clearance:0.03||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP4(YHR069C)|FD-Score:4.93|P-value:4.07E-7|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC10(YLR166C)|FD-Score:5.65|P-value:8.22E-9|Clearance:0.71||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SNU71(YGR013W)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.35||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:SSL1(YLR005W)|FD-Score:4.3|P-value:8.56E-6|Clearance:0.1||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SUI3(YPL237W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.14||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TAF11(YML015C)|FD-Score:3.85|P-value:5.95E-5|Clearance:0.09||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:UBC1(YDR177W)|FD-Score:4.89|P-value:5.06E-7|Clearance:0.59||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP25(YIL091C)|FD-Score:-3.87|P-value:5.43E-5|Clearance:0||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YGL069C(YGL069C_d)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.02||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24208661
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ABF2(YMR072W)|FD-Score:5.82|P-value:3.02E-9||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ADA2(YDR448W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADO1(YJR105W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ANP1(YEL036C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ATP2(YJR121W)|FD-Score:-4.35|P-value:6.85E-6||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ATP3(YBR039W)|FD-Score:4.3|P-value:8.57E-6||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:CLB5(YPR120C)|FD-Score:5.43|P-value:2.85E-8||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:CPR3(YML078W)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:CTF18(YMR078C)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTR1(YPR124W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DIF1(YLR437C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:EAF5(YEL018W)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:FMP16(YDR070C_p)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GET3(YDL100C)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GTR1(YML121W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GZF3(YJL110C)|FD-Score:4|P-value:3.18E-5||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAT1(YPL001W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HCM1(YCR065W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HFD1(YMR110C)|FD-Score:-4.51|P-value:3.22E-6||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:HOM2(YDR158W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC4(YDR540C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LCB5(YLR260W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MAC1(YMR021C)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MBP1(YDL056W)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MCM16(YPR046W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDL1(YLR188W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MIC17(YMR002W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRF1(YGL143C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRP4(YHL004W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRP51(YPL118W)|FD-Score:4.81|P-value:7.43E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL22(YNL177C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTC4(YBR255W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:POL32(YJR043C)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PYC2(YBR218C)|FD-Score:4.82|P-value:7.17E-7||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RDH54(YBR073W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RPL38(YLR325C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPL42B(YHR141C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTN2(YDL204W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAE3(YHR079C-A)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SAP1(YER047C)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SET4(YJL105W)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SFP1(YLR403W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SGM1(YJR134C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SRL1(YOR247W)|FD-Score:7.25|P-value:2.16E-13||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SUC2(YIL162W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:THP3(YPR045C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TLG2(YOL018C)|FD-Score:4.3|P-value:8.62E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP3(YKL211C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS51(YKR020W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VTH1(YIL173W)|FD-Score:-4.4|P-value:5.32E-6||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YEL045C(YEL045C_d)|FD-Score:4.99|P-value:2.99E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YHR125W(YHR125W_d)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL077C(YIL077C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YKL162C(YKL162C_p)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YLL054C(YLL054C_p)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YMR31(YFR049W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL010W(YNL010W_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL114C(YOL114C_p)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:AAD3(YCR107W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ABF2(YMR072W)|FD-Score:5.82|P-value:3.02E-9||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ADA2(YDR448W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADO1(YJR105W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ANP1(YEL036C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ATP2(YJR121W)|FD-Score:-4.35|P-value:6.85E-6||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ATP3(YBR039W)|FD-Score:4.3|P-value:8.57E-6||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:CLB5(YPR120C)|FD-Score:5.43|P-value:2.85E-8||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:CPR3(YML078W)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:CTF18(YMR078C)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTR1(YPR124W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DIF1(YLR437C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:EAF5(YEL018W)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:FMP16(YDR070C_p)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GET3(YDL100C)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GTR1(YML121W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GZF3(YJL110C)|FD-Score:4|P-value:3.18E-5||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAT1(YPL001W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HCM1(YCR065W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HFD1(YMR110C)|FD-Score:-4.51|P-value:3.22E-6||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:HOM2(YDR158W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC4(YDR540C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LCB5(YLR260W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MAC1(YMR021C)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MBP1(YDL056W)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MCM16(YPR046W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDL1(YLR188W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MIC17(YMR002W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRF1(YGL143C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRP4(YHL004W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRP51(YPL118W)|FD-Score:4.81|P-value:7.43E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL22(YNL177C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTC4(YBR255W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:POL32(YJR043C)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PYC2(YBR218C)|FD-Score:4.82|P-value:7.17E-7||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RDH54(YBR073W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RPL38(YLR325C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPL42B(YHR141C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTN2(YDL204W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAE3(YHR079C-A)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SAP1(YER047C)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SET4(YJL105W)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SFP1(YLR403W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SGM1(YJR134C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SRL1(YOR247W)|FD-Score:7.25|P-value:2.16E-13||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SUC2(YIL162W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:THP3(YPR045C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TLG2(YOL018C)|FD-Score:4.3|P-value:8.62E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP3(YKL211C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS51(YKR020W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VTH1(YIL173W)|FD-Score:-4.4|P-value:5.32E-6||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YEL045C(YEL045C_d)|FD-Score:4.99|P-value:2.99E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YHR125W(YHR125W_d)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL077C(YIL077C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YKL162C(YKL162C_p)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YLL054C(YLL054C_p)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YMR31(YFR049W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL010W(YNL010W_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL114C(YOL114C_p)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C10.201.03E-244.55RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR166C5.658.22E-90.71SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YHR069C4.934.07E-70.04RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YDR177W4.895.06E-70.59UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YLR005W4.308.56E-60.10SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YGR013W4.201.36E-50.35SNU71Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
YML015C3.855.95E-50.09TAF11TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
YGR191W3.768.63E-50.00HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YOR210W3.758.74E-50.03RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YKR022C3.729.89E-50.15NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YGR145W3.571.79E-40.03ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YPL237W3.541.98E-40.14SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YIL004C3.403.40E-40.07BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YMR128W3.334.40E-40.01ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YOL005C3.314.59E-40.02RPB11RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR247W7.252.16E-13SRL1Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication
YMR072W5.823.02E-9ABF2Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation
YJL105W5.571.24E-8SET4Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication
YPR120C5.432.85E-8CLB5B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase
YEL045C_d4.992.99E-7YEL045C_dDubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress
YBR218C4.827.17E-7PYC2Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
YPL118W4.817.43E-7MRP51Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
YDR158W4.641.74E-6HOM2Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YML078W4.434.64E-6CPR3Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria
YMR021C4.385.88E-6MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YBR039W4.308.57E-6ATP3Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YOL018C4.308.62E-6TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YNL177C4.181.44E-5MRPL22Mitochondrial ribosomal protein of the large subunit
YKL211C4.141.72E-5TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YJR043C4.131.84E-5POL32Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p

GO enrichment analysis for SGTC_1964
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1352.62E-25SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0588235RPP1 & pyrimidine depletion
0.1316.29E-24SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.157143
0.1241.04E-21SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.107143RPP1 & pyrimidine depletion
0.1233.16E-21SGTC_23067482397 179.5 μMChembridge (Fragment library)8914480.130435RPP1 & pyrimidine depletion
0.1181.35E-19SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.15625
0.1164.65E-19SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.131148copper-dependent oxidative stress
0.1151.12E-18SGTC_13611608-0041 5.6 μMChemDiv (Drug-like library)X13610.128571RPP1 & pyrimidine depletion
0.1133.08E-18SGTC_31699102970 49.5 μMChembridge (Drug-like library)170278390.125RPP1 & pyrimidine depletion
0.1119.30E-18SGTC_3620310-0100 9.0 μMChemDiv (Drug-like library)7902350.135135RPP1 & pyrimidine depletion
0.1111.21E-17SGTC_389tpck 16.7 μMMiscellaneous4396470.150685
0.1111.55E-17SGTC_6671498-1044 59.0 μMChemDiv (Drug-like library)67399820.108108RPP1 & pyrimidine depletion
0.1102.14E-17SGTC_2686alizarin 95.4 μMMiscellaneous62930.112903RPP1 & pyrimidine depletion
0.1102.18E-17SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.118421TSC3-RPN4
0.1102.96E-17SGTC_31129123419 49.5 μMChembridge (Drug-like library)170158540.125RPP1 & pyrimidine depletion
0.1081.05E-16SGTC_8201582-0060 59.9 μMChemDiv (Drug-like library)38488400.138889

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_22047233102200 μM0.4385962172480Chembridge (Fragment library)229.231341.6841360S ribosome export
SGTC_1628st00425277.7 μM0.413793692968TimTec (Natural product derivative library)257.28452.4713
SGTC_8810833-0569170 μM0.3934435418700ChemDiv (Drug-like library)306.315362.68414
SGTC_1971st06942963.5 μM0.3809521246522TimTec (Natural product derivative library)280.278082.5191460S ribosome export
SGTC_1856st05705455.7 μM0.3731341266018TimTec (Natural product derivative library)359.3498634.36814
SGTC_13671812-051076.9 μM0.3676471142582ChemDiv (Drug-like library)410.217584.12614
SGTC_2847900566171.43 μM0.3606562991449Chembridge (Drug-like library)303.740263.42913
SGTC_20875276107200 μM0.357143144405Chembridge (Fragment library)238.670241.51713
SGTC_1655st01254655.1 μM0.344828720985TimTec (Natural product derivative library)237.253342.63113
SGTC_1821st05346565.6 μM0.333333675086TimTec (Natural product derivative library)282.090121.81613