Pectolinarigenin

5,7-dihydroxy-6-methoxy-2-(4-methoxyphenyl)chromen-4-one

An anti-inflammatory.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1965
Screen concentration 58.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 5320438
SMILES COC1=CC=C(C=C1)C2=CC(=O)C3=C(C(=C(C=C3O2)O)OC)O
Standardized SMILES COc1ccc(cc1)C2=CC(=O)c3c(O)c(OC)c(O)cc3O2
Molecular weight 314.2895
ALogP 2.62
H-bond donor count 2
H-bond acceptor count 6
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.29
% growth inhibition (Hom. pool) 4.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5320438
Download HIP data (tab-delimited text)  (excel)
Gene:DPS1(YLL018C)|FD-Score:-3.1|P-value:9.63E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:INO80(YGL150C)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.07||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:MCM6(YGL201C)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.12||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:RPP1(YHR062C)|FD-Score:4.91|P-value:4.61E-7|Clearance:0.91||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRB1(YMR131C)|FD-Score:-3.5|P-value:2.32E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRP9(YPR137W)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC13(YLR208W)|FD-Score:7.37|P-value:8.54E-14|Clearance:1.87||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TAD3(YLR316C)|FD-Score:4|P-value:3.17E-5|Clearance:0.19||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TAF10(YDR167W)|FD-Score:3.81|P-value:7.06E-5|Clearance:0.3||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF11(YML015C)|FD-Score:3.51|P-value:2.28E-4|Clearance:0.29||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:YKT6(YKL196C)|FD-Score:5.5|P-value:1.86E-8|Clearance:0.6||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:DPS1(YLL018C)|FD-Score:-3.1|P-value:9.63E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:INO80(YGL150C)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.07||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:MCM6(YGL201C)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.12||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:RPP1(YHR062C)|FD-Score:4.91|P-value:4.61E-7|Clearance:0.91||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRB1(YMR131C)|FD-Score:-3.5|P-value:2.32E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRP9(YPR137W)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC13(YLR208W)|FD-Score:7.37|P-value:8.54E-14|Clearance:1.87||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TAD3(YLR316C)|FD-Score:4|P-value:3.17E-5|Clearance:0.19||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TAF10(YDR167W)|FD-Score:3.81|P-value:7.06E-5|Clearance:0.3||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF11(YML015C)|FD-Score:3.51|P-value:2.28E-4|Clearance:0.29||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:YKT6(YKL196C)|FD-Score:5.5|P-value:1.86E-8|Clearance:0.6||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5320438
Download HOP data (tab-delimited text)  (excel)
Gene:AIM17(YHL021C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AKR2(YOR034C)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:ANR2(YKL047W_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ARO1(YDR127W)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATP3(YBR039W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCH1(YMR237W)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BRR1(YPR057W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BSC1(YDL037C)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CTK3(YML112W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DSS1(YMR287C)|FD-Score:5.7|P-value:5.82E-9||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EGT2(YNL327W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:ERV46(YAL042W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FLC3(YGL139W)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GAL2(YLR081W)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GPD1(YDL022W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:IRC21(YMR073C)|FD-Score:5.26|P-value:7.06E-8||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LCB4(YOR171C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MDY2(YOL111C)|FD-Score:3.84|P-value:6.03E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MFA2(YNL145W)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MSA2(YKR077W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSL1(YIR009W)|FD-Score:5.6|P-value:1.06E-8||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:NTH2(YBR001C)|FD-Score:5.32|P-value:5.19E-8||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PDH1(YPR002W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PHO89(YBR296C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PRM10(YJL108C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:RIM1(YCR028C-A)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:ROX1(YPR065W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPS29A(YLR388W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RSM28(YDR494W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SDC1(YDR469W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SFT2(YBL102W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:TDA5(YLR426W_p)|FD-Score:5.08|P-value:1.87E-7||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:THO2(YNL139C)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TIR4(YOR009W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:TPN1(YGL186C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRE1(YPL176C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:VPS71(YML041C)|FD-Score:5.33|P-value:5.03E-8||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VTH1(YIL173W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YGL214W(YGL214W_d)|FD-Score:-4.37|P-value:6.25E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGP1(YNL160W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR153W(YGR153W_p)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function Gene:YHR125W(YHR125W_d)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR177W(YHR177W_p)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YML012C-A(YML012C-A_d)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YPR196W(YPR196W_p)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Putative maltose-responsive transcription factor Gene:YRM1(YOR172W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes Gene:ZTA1(YBR046C)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:AIM17(YHL021C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AKR2(YOR034C)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:ANR2(YKL047W_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ARO1(YDR127W)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATP3(YBR039W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCH1(YMR237W)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BRR1(YPR057W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BSC1(YDL037C)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CTK3(YML112W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DSS1(YMR287C)|FD-Score:5.7|P-value:5.82E-9||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EGT2(YNL327W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:ERV46(YAL042W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FLC3(YGL139W)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GAL2(YLR081W)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GPD1(YDL022W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:IRC21(YMR073C)|FD-Score:5.26|P-value:7.06E-8||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LCB4(YOR171C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MDY2(YOL111C)|FD-Score:3.84|P-value:6.03E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MFA2(YNL145W)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MSA2(YKR077W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSL1(YIR009W)|FD-Score:5.6|P-value:1.06E-8||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:NTH2(YBR001C)|FD-Score:5.32|P-value:5.19E-8||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PDH1(YPR002W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PHO89(YBR296C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PRM10(YJL108C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:RIM1(YCR028C-A)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:ROX1(YPR065W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPS29A(YLR388W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RSM28(YDR494W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SDC1(YDR469W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SFT2(YBL102W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:TDA5(YLR426W_p)|FD-Score:5.08|P-value:1.87E-7||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:THO2(YNL139C)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TIR4(YOR009W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:TPN1(YGL186C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRE1(YPL176C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:VPS71(YML041C)|FD-Score:5.33|P-value:5.03E-8||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VTH1(YIL173W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YGL214W(YGL214W_d)|FD-Score:-4.37|P-value:6.25E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGP1(YNL160W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR153W(YGR153W_p)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function Gene:YHR125W(YHR125W_d)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR177W(YHR177W_p)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YML012C-A(YML012C-A_d)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YPR196W(YPR196W_p)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Putative maltose-responsive transcription factor Gene:YRM1(YOR172W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes Gene:ZTA1(YBR046C)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W7.378.54E-141.87SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YKL196C5.501.86E-80.60YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YHR062C4.914.61E-70.91RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR316C4.003.17E-50.19TAD3Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs
YDR167W3.817.06E-50.30TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YML015C3.512.28E-40.29TAF11TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
YGL150C3.216.56E-40.07INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YGL201C3.158.29E-40.12MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YJR076C3.020.001260.00CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YBR121C3.020.001280.25GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YDL153C2.770.002800.06SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YDR429C2.710.003330.07TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YJL194W2.650.004080.00CDC6Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR222C2.640.004100.00UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YOR119C2.640.004120.02RIO1Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR287C5.705.82E-9DSS13'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
YNL139C5.601.05E-8THO2Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
YIR009W5.601.06E-8MSL1U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
YML041C5.335.03E-8VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YBR001C5.325.19E-8NTH2Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication
YMR073C5.267.06E-8IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YLR426W_p5.081.87E-7TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YLR081W4.641.72E-6GAL2Galactose permease, required for utilization of galactose; also able to transport glucose
YMR237W4.562.58E-6BCH1Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication
YBR039W4.161.56E-5ATP3Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YNL327W4.121.88E-5EGT2Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner
YPR057W4.111.97E-5BRR1snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed
YNL145W4.102.10E-5MFA2Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
YPR196W_p3.993.35E-5YPR196W_pPutative maltose-responsive transcription factor
YBR046C3.953.91E-5ZTA1NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

GO enrichment analysis for SGTC_1965
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1802.03E-44SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0810811
0.1791.29E-43SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.0533333
0.1741.73E-41SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.104478
0.1504.80E-31SGTC_14163966-0321 25.3 μMChemDiv (Drug-like library)5825300.0793651
0.1395.03E-27SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.134328
0.1382.57E-26SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.520833
0.1339.80E-25SGTC_7864544-0069 423.0 μMChemDiv (Drug-like library)7488210.125
0.1286.25E-23SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.177419
0.1057.52E-16SGTC_14884013-0813 75.9 μMChemDiv (Drug-like library)29100570.121622RPP1 & pyrimidine depletion
0.1031.57E-15SGTC_9933909-8757 62.7 μMChemDiv (Drug-like library)42883240.0694444
0.0992.15E-14SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0869565
0.0912.97E-12SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.128571
0.0905.19E-12SGTC_21745790901 200.0 μMChembridge (Fragment library)7860200.0869565tubulin folding & SWR complex
0.0881.15E-11SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.0769231ergosterol biosynthesis
0.0856.05E-11SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.102564RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2636apigenin dimethyl ether100 μM0.7111115281601Microsource (Natural product library)298.290062.86115
SGTC_2600tangeritin87.95 μM0.57446868077Microsource (Natural product library)372.36863.05407
SGTC_1860st05622874.6 μM0.571429688679TimTec (Natural product derivative library)268.264082.87814
SGTC_1834st05598152.8 μM0.56471719TimTec (Natural product derivative library)284.263482.63625RPP1 & pyrimidine depletion
SGTC_2513baicalein25.65 μM0.5531915281605Microsource (Natural product library)270.23692.4135calcium & mitochondrial duress
SGTC_15924',5'-dihydroxyflavone8.85 μM0.55102688669TimTec (Pure natural product library)282.290663.1030460S ribosome export
SGTC_1111tnp00062510.7 nM0.54676306TimTec (Natural product library)268.264082.87814
SGTC_1955wogonin32.4 μM0.5294125281703TimTec (Natural product derivative library)284.263482.63625
SGTC_14204'-methoxyflavone42 μM0.52083377793ChemDiv (Drug-like library)252.264683.1203
SGTC_26344'-methoxyflavone100 μM0.52083377793Microsource (Natural product library)252.264683.1203
SGTC_1852st05625431.4 μM0.509804442583TimTec (Natural product derivative library)312.316643.08705