st069590

2-(1-methoxycarbonylindol-3-yl)ethyl-methylidyneazanium

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1968
Screen concentration 43.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 6384066
SMILES COC(=O)N1C=C(C2=CC=CC=C21)CC[N+]#C
Standardized SMILES COC(=O)n1cc(CC[N+]#C)c2ccccc12
Molecular weight 229.2545
ALogP 3.03
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 57.38
% growth inhibition (Hom. pool) 9.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6384066
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:-4.58|P-value:2.32E-6|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:8.52|P-value:7.98E-18|Clearance:0.95||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BIG1(YHR101C)|FD-Score:5.55|P-value:1.43E-8|Clearance:0.53||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:BPL1(YDL141W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.06||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:CDC12(YHR107C)|FD-Score:9.83|P-value:4.28E-23|Clearance:1.31||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC25(YLR310C)|FD-Score:3.19|P-value:7.20E-4|Clearance:0.01||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC31(YOR257W)|FD-Score:-5.06|P-value:2.07E-7|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DCP2(YNL118C)|FD-Score:4.79|P-value:8.51E-7|Clearance:0.6||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:DML1(YMR211W)|FD-Score:11|P-value:1.39E-28|Clearance:1.2||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG7(YHR072W)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.05||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GAA1(YLR088W)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.4||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GDI1(YER136W)|FD-Score:5.01|P-value:2.65E-7|Clearance:0.17||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:IRA1(YBR140C)|FD-Score:3.27|P-value:5.38E-4|Clearance:0.04||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KEI1(YDR367W)|FD-Score:3.34|P-value:4.23E-4|Clearance:0.07||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MAS1(YLR163C)|FD-Score:-4.11|P-value:1.98E-5|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MPE1(YKL059C)|FD-Score:4.85|P-value:6.22E-7|Clearance:0.06||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOP58(YOR310C)|FD-Score:-6.34|P-value:1.13E-10|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PDC2(YDR081C)|FD-Score:5.97|P-value:1.17E-9|Clearance:0.42||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRE5(YMR314W)|FD-Score:3.76|P-value:8.46E-5|Clearance:0.06||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RNA15(YGL044C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.09||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB7(YDR404C)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPG1(YBR079C)|FD-Score:3.18|P-value:7.36E-4|Clearance:0.11||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:RPN5(YDL147W)|FD-Score:3.79|P-value:7.53E-5|Clearance:0.03||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPS15(YOL040C)|FD-Score:-3.97|P-value:3.54E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSM10(YDR041W)|FD-Score:-3.36|P-value:3.85E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SMC5(YOL034W)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.02||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SPC105(YGL093W)|FD-Score:16.8|P-value:1.22E-63|Clearance:4.18||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TOM22(YNL131W)|FD-Score:-3.44|P-value:2.86E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:-3.15|P-value:8.08E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:WRS1(YOL097C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.16||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YDR526C(YDR526C_d)|FD-Score:15.2|P-value:1.46E-52|Clearance:4.18||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL086C(YJL086C_d)|FD-Score:-3.17|P-value:7.66E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YJL195C(YJL195C_d)|FD-Score:-3.16|P-value:7.96E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJR141W(YJR141W_p)|FD-Score:7.57|P-value:1.86E-14|Clearance:1.6||SGD DESC:Essential protein of unknown function Gene:YKT6(YKL196C)|FD-Score:3.21|P-value:6.73E-4|Clearance:0.02||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YTH1(YPR107C)|FD-Score:18|P-value:8.78E-73|Clearance:4.18||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:-4.58|P-value:2.32E-6|Clearance:0||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:8.52|P-value:7.98E-18|Clearance:0.95||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BIG1(YHR101C)|FD-Score:5.55|P-value:1.43E-8|Clearance:0.53||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:BPL1(YDL141W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.06||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:CDC12(YHR107C)|FD-Score:9.83|P-value:4.28E-23|Clearance:1.31||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC25(YLR310C)|FD-Score:3.19|P-value:7.20E-4|Clearance:0.01||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC31(YOR257W)|FD-Score:-5.06|P-value:2.07E-7|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DCP2(YNL118C)|FD-Score:4.79|P-value:8.51E-7|Clearance:0.6||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:DML1(YMR211W)|FD-Score:11|P-value:1.39E-28|Clearance:1.2||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG7(YHR072W)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.05||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GAA1(YLR088W)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.4||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GDI1(YER136W)|FD-Score:5.01|P-value:2.65E-7|Clearance:0.17||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:IRA1(YBR140C)|FD-Score:3.27|P-value:5.38E-4|Clearance:0.04||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KEI1(YDR367W)|FD-Score:3.34|P-value:4.23E-4|Clearance:0.07||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MAS1(YLR163C)|FD-Score:-4.11|P-value:1.98E-5|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MPE1(YKL059C)|FD-Score:4.85|P-value:6.22E-7|Clearance:0.06||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOP58(YOR310C)|FD-Score:-6.34|P-value:1.13E-10|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PDC2(YDR081C)|FD-Score:5.97|P-value:1.17E-9|Clearance:0.42||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRE5(YMR314W)|FD-Score:3.76|P-value:8.46E-5|Clearance:0.06||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RNA15(YGL044C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.09||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB7(YDR404C)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPG1(YBR079C)|FD-Score:3.18|P-value:7.36E-4|Clearance:0.11||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:RPN5(YDL147W)|FD-Score:3.79|P-value:7.53E-5|Clearance:0.03||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPS15(YOL040C)|FD-Score:-3.97|P-value:3.54E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSM10(YDR041W)|FD-Score:-3.36|P-value:3.85E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SMC5(YOL034W)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.02||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SPC105(YGL093W)|FD-Score:16.8|P-value:1.22E-63|Clearance:4.18||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TOM22(YNL131W)|FD-Score:-3.44|P-value:2.86E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:-3.15|P-value:8.08E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:WRS1(YOL097C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.16||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YDR526C(YDR526C_d)|FD-Score:15.2|P-value:1.46E-52|Clearance:4.18||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL086C(YJL086C_d)|FD-Score:-3.17|P-value:7.66E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YJL195C(YJL195C_d)|FD-Score:-3.16|P-value:7.96E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJR141W(YJR141W_p)|FD-Score:7.57|P-value:1.86E-14|Clearance:1.6||SGD DESC:Essential protein of unknown function Gene:YKT6(YKL196C)|FD-Score:3.21|P-value:6.73E-4|Clearance:0.02||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YTH1(YPR107C)|FD-Score:18|P-value:8.78E-73|Clearance:4.18||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6384066
Download HOP data (tab-delimited text)  (excel)
Gene:AIM17(YHL021C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM18(YHR198C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARC18(YLR370C)|FD-Score:-3.19|P-value:7.05E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG20(YDL113C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:BRR1(YPR057W)|FD-Score:-5.84|P-value:2.67E-9||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CBF1(YJR060W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CKB2(YOR039W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CRS5(YOR031W)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CTR1(YPR124W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYK3(YDL117W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:DOC1(YGL240W)|FD-Score:-5.21|P-value:9.19E-8||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:EGD1(YPL037C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:FPR2(YDR519W)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GDT1(YBR187W_p)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GEP5(YLR091W)|FD-Score:-3.23|P-value:6.30E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:ISM1(YPL040C)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:KIN82(YCR091W)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:LST4(YKL176C)|FD-Score:-4|P-value:3.23E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYP1(YNL268W)|FD-Score:6.46|P-value:5.38E-11||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MOS1(YCL057C-A)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL31(YKL138C)|FD-Score:-4.76|P-value:9.77E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTG1(YMR097C)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MYO4(YAL029C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NAM8(YHR086W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NAT4(YMR069W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:PAN5(YHR063C)|FD-Score:4.32|P-value:7.71E-6||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PAU7(YAR020C)|FD-Score:5.52|P-value:1.68E-8||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PDR12(YPL058C)|FD-Score:4.35|P-value:6.92E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PET10(YKR046C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PGD1(YGL025C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIF1(YML061C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:QRI5(YLR204W)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD9(YDR217C)|FD-Score:4.76|P-value:9.62E-7||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RPL6B(YLR448W)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-3.96|P-value:3.73E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RRP6(YOR001W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SAP1(YER047C)|FD-Score:4.95|P-value:3.67E-7||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SIZ1(YDR409W)|FD-Score:5.12|P-value:1.55E-7||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLH1(YGR271W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SPS4(YOR313C)|FD-Score:5.82|P-value:3.01E-9||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:TED1(YIL039W)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRM9(YML014W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TSC3(YBR058C-A)|FD-Score:-3.79|P-value:7.60E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBA4(YHR111W)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VPS1(YKR001C)|FD-Score:-5.1|P-value:1.73E-7||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YCL023C(YCL023C_d)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCR006C(YCR006C_d)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR061W(YDR061W_p)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR114C(YDR114C_p)|FD-Score:-4.58|P-value:2.38E-6||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR338C(YDR338C_p)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YDR340W(YDR340W_d)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Putative protein of unknown function Gene:YEN1(YER041W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFT2(YDR319C_p)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGL072C(YGL072C_d)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL159W(YGL159W_p)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHR078W(YHR078W_p)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YKR047W(YKR047W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YNL296W(YNL296W_d)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL073C(YOL073C_p)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YPL168W(YPL168W_p)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YTA6(YPL074W)|FD-Score:4.76|P-value:9.58E-7||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:AIM17(YHL021C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM18(YHR198C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARC18(YLR370C)|FD-Score:-3.19|P-value:7.05E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG20(YDL113C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:BRR1(YPR057W)|FD-Score:-5.84|P-value:2.67E-9||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CBF1(YJR060W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CKB2(YOR039W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CRS5(YOR031W)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CTR1(YPR124W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYK3(YDL117W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:DOC1(YGL240W)|FD-Score:-5.21|P-value:9.19E-8||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:EGD1(YPL037C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:FPR2(YDR519W)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GDT1(YBR187W_p)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GEP5(YLR091W)|FD-Score:-3.23|P-value:6.30E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:ISM1(YPL040C)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:KIN82(YCR091W)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:LST4(YKL176C)|FD-Score:-4|P-value:3.23E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYP1(YNL268W)|FD-Score:6.46|P-value:5.38E-11||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MOS1(YCL057C-A)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL31(YKL138C)|FD-Score:-4.76|P-value:9.77E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTG1(YMR097C)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MYO4(YAL029C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NAM8(YHR086W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NAT4(YMR069W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:PAN5(YHR063C)|FD-Score:4.32|P-value:7.71E-6||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PAU7(YAR020C)|FD-Score:5.52|P-value:1.68E-8||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PDR12(YPL058C)|FD-Score:4.35|P-value:6.92E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PET10(YKR046C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PGD1(YGL025C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIF1(YML061C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:QRI5(YLR204W)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD9(YDR217C)|FD-Score:4.76|P-value:9.62E-7||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RPL6B(YLR448W)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-3.96|P-value:3.73E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RRP6(YOR001W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SAP1(YER047C)|FD-Score:4.95|P-value:3.67E-7||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SIZ1(YDR409W)|FD-Score:5.12|P-value:1.55E-7||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLH1(YGR271W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SPS4(YOR313C)|FD-Score:5.82|P-value:3.01E-9||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:TED1(YIL039W)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRM9(YML014W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TSC3(YBR058C-A)|FD-Score:-3.79|P-value:7.60E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBA4(YHR111W)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VPS1(YKR001C)|FD-Score:-5.1|P-value:1.73E-7||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YCL023C(YCL023C_d)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YCR006C(YCR006C_d)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR061W(YDR061W_p)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR114C(YDR114C_p)|FD-Score:-4.58|P-value:2.38E-6||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR338C(YDR338C_p)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YDR340W(YDR340W_d)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Putative protein of unknown function Gene:YEN1(YER041W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFT2(YDR319C_p)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGL072C(YGL072C_d)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL159W(YGL159W_p)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHR078W(YHR078W_p)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YKR047W(YKR047W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YNL296W(YNL296W_d)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL073C(YOL073C_p)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YPL168W(YPL168W_p)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YTA6(YPL074W)|FD-Score:4.76|P-value:9.58E-7||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C18.008.78E-734.18YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YGL093W16.801.22E-634.18SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YDR526C_d15.201.46E-524.18YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR211W11.001.39E-281.20DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YHR107C9.834.28E-231.31CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YJL081C8.527.98E-180.95ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YJR141W_p7.571.86E-141.60YJR141W_pEssential protein of unknown function
YDR081C5.971.17E-90.42PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YHR101C5.551.43E-80.54BIG1Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
YER136W5.012.65E-70.17GDI1GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
YKL059C4.856.22E-70.06MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YNL118C4.798.51E-70.60DCP2Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
YLR088W4.191.39E-50.40GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YDL147W3.797.53E-50.03RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YMR314W3.768.46E-50.06PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL268W6.465.38E-11LYP1Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
YOR313C5.823.01E-9SPS4Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage
YOR031W5.601.10E-8CRS5Copper-binding metallothionein, required for wild-type copper resistance
YAR020C5.521.68E-8PAU7Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
YDR409W5.121.55E-7SIZ1SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
YER047C4.953.67E-7SAP1Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system
YPL074W4.769.58E-7YTA6Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
YDR217C4.769.62E-7RAD9DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
YPL058C4.356.92E-6PDR12Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity
YHR063C4.327.71E-6PAN52-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE
YMR069W4.221.24E-5NAT4N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A
YHR198C4.191.41E-5AIM18Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YDR319C_p4.151.69E-5YFT2_pProtein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
YNL296W_d4.082.25E-5YNL296W_dDubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog
YDR338C_p3.934.22E-5YDR338C_pPutative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily

GO enrichment analysis for SGTC_1968
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3038.55E-125SGTC_30641327-0027 10.0 μMChemDiv (Drug-like library)160004550.0945946
0.2929.07E-117SGTC_24295902844 64.4 μMMiscellaneous19277900.0909091
0.2841.17E-109SGTC_30591327-0034 3.0 μMChemDiv (Drug-like library)159985800.0519481
0.2562.46E-88SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.0519481
0.2431.90E-79SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004550.0945946
0.1738.64E-41SGTC_216k038-0035 28.4 μMChemDiv (Drug-like library)44442310.0434783ubiquinone biosynthesis & proteasome
0.1603.46E-35SGTC_200k038-0079 37.5 μMChemDiv (Drug-like library)34366830.0555556ubiquinone biosynthesis & proteasome
0.1577.39E-34SGTC_5860957-0265 8.2 μMChemDiv (Drug-like library)67523090.091954cell wall
0.1555.20E-33SGTC_5801123-0028 41.0 μMChemDiv (Drug-like library)16772480.056338DNA damage response
0.1541.03E-32SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.132075SWF1 & branched chain AA biosynthesis
0.1506.36E-31SGTC_451chloral hydrate 100.0 μMMiscellaneous2707NA
0.1491.64E-30SGTC_294k038-0071 157.0 μMChemDiv (Drug-like library)241528260.0555556ubiquinone biosynthesis & proteasome
0.1453.82E-29SGTC_268camptothecin 39.6 μMMiscellaneous243600.0941176DNA damage response
0.1411.80E-27SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.128571RSC complex & mRNA processing
0.1403.05E-27SGTC_12941000-1494 117.0 μMChemDiv (Drug-like library)7127970.1875

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8350312-001067.2 μM0.3148151626196ChemDiv (Drug-like library)213.231942.23302
SGTC_10503232-115669.3 μM0.314815700529ChemDiv (Drug-like library)237.276523.0311RSC complex & mRNA processing
SGTC_7924534-1957155 μM0.2786891717904ChemDiv (Drug-like library)282.337022.73412
SGTC_22577960413200 μM0.252974689Chembridge (Fragment library)237.725242.53122NEO1-PIK1
SGTC_229benomyl22.87 μM0.24242428780Miscellaneous290.317722.96524tubulin folding & SWR complex
SGTC_12730849-009614.1 μM0.2413795286025ChemDiv (Drug-like library)216.235882.82302RPP1 & pyrimidine depletion
SGTC_1998st07579776.8 μM0.23437524208315TimTec (Natural product derivative library)260.33153.40722mitochondrial response to ROS
SGTC_14153966-007178.1 μM0.2307692893185ChemDiv (Drug-like library)251.279923.53202DNA intercalators
SGTC_2932901671144.54 μM0.2307697117328Chembridge (Drug-like library)308.331242.78423
SGTC_2788528273471.43 μM0.225806743706Chembridge (Drug-like library)283.282023.05605