st069479

2,3,4,6,7,8,9,9a-octahydro-1H-quinolizin-1-ylmethyl 3-chloro-5-thiophen-2-yl-7-(trifluoromethyl)pyrazolo[1,5-a]pyrimidine-2-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1969
Screen concentration 40.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 3895485
SMILES C1CCN2CCCC(C2C1)COC(=O)C3=NN4C(=CC(=NC4=C3Cl)C5=CC=CS5)C(F)(F)F
Standardized SMILES FC(F)(F)c1cc(nc2c(Cl)c(nn12)C(=O)OCC3CCCN4CCCCC34)c5cccs5
Molecular weight 498.9489
ALogP 5.96
H-bond donor count 0
H-bond acceptor count 9
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.28
% growth inhibition (Hom. pool) 1.09


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3895485
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.07||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ATP16(YDL004W)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.13||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:DSN1(YIR010W)|FD-Score:-4|P-value:3.19E-5|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:FAP7(YDL166C)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:KRE33(YNL132W)|FD-Score:-3.79|P-value:7.67E-5|Clearance:0||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MET30(YIL046W)|FD-Score:3.16|P-value:7.84E-4|Clearance:0.05||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MEX67(YPL169C)|FD-Score:3.8|P-value:7.26E-5|Clearance:0.28||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NPA3(YJR072C)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.12||SGD DESC:Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress Gene:PRE3(YJL001W)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PRI2(YKL045W)|FD-Score:-3.21|P-value:6.54E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP16(YKR086W)|FD-Score:3.24|P-value:6.06E-4|Clearance:0||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:PRP2(YNR011C)|FD-Score:5.65|P-value:7.96E-9|Clearance:1.85||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RPN5(YDL147W)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.03||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPP1(YHR062C)|FD-Score:-4.1|P-value:2.02E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RTT105(YER104W)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.19||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEN34(YAR008W)|FD-Score:-3.15|P-value:8.25E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:TAH18(YPR048W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:ARP7(YPR034W)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.07||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ATP16(YDL004W)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.13||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:DSN1(YIR010W)|FD-Score:-4|P-value:3.19E-5|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:FAP7(YDL166C)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:KRE33(YNL132W)|FD-Score:-3.79|P-value:7.67E-5|Clearance:0||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MET30(YIL046W)|FD-Score:3.16|P-value:7.84E-4|Clearance:0.05||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MEX67(YPL169C)|FD-Score:3.8|P-value:7.26E-5|Clearance:0.28||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NPA3(YJR072C)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.12||SGD DESC:Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress Gene:PRE3(YJL001W)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PRI2(YKL045W)|FD-Score:-3.21|P-value:6.54E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP16(YKR086W)|FD-Score:3.24|P-value:6.06E-4|Clearance:0||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:PRP2(YNR011C)|FD-Score:5.65|P-value:7.96E-9|Clearance:1.85||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RPN5(YDL147W)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.03||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPP1(YHR062C)|FD-Score:-4.1|P-value:2.02E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RTT105(YER104W)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.19||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEN34(YAR008W)|FD-Score:-3.15|P-value:8.25E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:TAH18(YPR048W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3895485
Download HOP data (tab-delimited text)  (excel)
Gene:ACN9(YDR511W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:APL4(YPR029C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:ATP15(YPL271W)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BIO2(YGR286C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BUD19(YJL188C_d)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CLB1(YGR108W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COX18(YGR062C)|FD-Score:4.44|P-value:4.56E-6||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DAL7(YIR031C)|FD-Score:4.34|P-value:7.27E-6||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DLS1(YJL065C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication Gene:FBP26(YJL155C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:GND1(YHR183W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GSF2(YML048W)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GTS1(YGL181W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HOP2(YGL033W)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IST3(YIR005W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:LRE1(YCL051W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:NTH2(YBR001C)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAU7(YAR020C)|FD-Score:-5.56|P-value:1.33E-8||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PBP2(YBR233W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PCS60(YBR222C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PEX32(YBR168W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:RAD9(YDR217C)|FD-Score:6.04|P-value:7.80E-10||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RCF2(YNR018W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RPL39(YJL189W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:SCP1(YOR367W)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLX8(YER116C)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SNL1(YIL016W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SNX4(YJL036W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOL4(YGR248W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SST2(YLR452C)|FD-Score:4|P-value:3.15E-5||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:SUC2(YIL162W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TOM71(YHR117W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TPK2(YPL203W)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPN1(YGL186C)|FD-Score:8.14|P-value:1.97E-16||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:VMA16(YHR026W)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YHR078W(YHR078W_p)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YHR177W(YHR177W_p)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIL046W-A(YIL046W-A_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YND1(YER005W)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL057W(YNL057W_d)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL257W(YPL257W_p)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR109W(YPR109W_p)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR197C(YPR197C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACN9(YDR511W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:APL4(YPR029C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:ATP15(YPL271W)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BIO2(YGR286C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BUD19(YJL188C_d)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CLB1(YGR108W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COX18(YGR062C)|FD-Score:4.44|P-value:4.56E-6||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DAL7(YIR031C)|FD-Score:4.34|P-value:7.27E-6||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DLS1(YJL065C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication Gene:FBP26(YJL155C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:GND1(YHR183W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GSF2(YML048W)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GTS1(YGL181W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HOP2(YGL033W)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IST3(YIR005W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:LRE1(YCL051W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:NTH2(YBR001C)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAU7(YAR020C)|FD-Score:-5.56|P-value:1.33E-8||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PBP2(YBR233W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PCS60(YBR222C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PEX32(YBR168W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:RAD9(YDR217C)|FD-Score:6.04|P-value:7.80E-10||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RCF2(YNR018W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RPL39(YJL189W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:SCP1(YOR367W)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLX8(YER116C)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SNL1(YIL016W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SNX4(YJL036W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOL4(YGR248W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SST2(YLR452C)|FD-Score:4|P-value:3.15E-5||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:SUC2(YIL162W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TOM71(YHR117W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TPK2(YPL203W)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPN1(YGL186C)|FD-Score:8.14|P-value:1.97E-16||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:VMA16(YHR026W)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YHR078W(YHR078W_p)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YHR177W(YHR177W_p)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIL046W-A(YIL046W-A_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YND1(YER005W)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL057W(YNL057W_d)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL257W(YPL257W_p)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR109W(YPR109W_p)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR197C(YPR197C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNR011C5.657.96E-91.85PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YPL169C3.807.26E-50.28MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YDL147W3.522.14E-40.03RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YDL004W3.502.36E-40.13ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YJR072C3.363.87E-40.12NPA3Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
YPR048W3.246.03E-40.00TAH18Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance
YKR086W3.246.06E-40.00PRP16DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step
YPR034W3.236.09E-40.07ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YIL046W3.167.84E-40.05MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YER104W3.119.44E-40.19RTT105Protein with a role in regulation of Ty1 transposition
YOR117W2.920.001750.02RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YGR047C2.900.001860.04TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YOR207C2.860.002130.12RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDR082W2.740.003100.01STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YBR202W2.730.003160.07MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL186C8.141.97E-16TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YDR217C6.047.80E-10RAD9DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
YHR026W5.191.07E-7VMA16Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
YML048W4.751.01E-6GSF2ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression
YJL189W4.691.34E-6RPL39Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
YGR062C4.444.56E-6COX18Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC
YOR367W4.434.72E-6SCP1Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin
YIR031C4.347.27E-6DAL7Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YJL155C4.121.90E-5FBP26Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
YHR078W_p4.082.27E-5YHR078W_pHigh osmolarity-regulated gene of unknown function
YLR452C4.003.15E-5SST2GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
YBR168W3.944.08E-5PEX32Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
YHR183W3.855.83E-5GND16-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication
YBR222C3.855.95E-5PCS60Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase
YPL203W3.817.05E-5TPK2cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase

GO enrichment analysis for SGTC_1969
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0854.73E-11SGTC_7181130-0073 349.0 μMChemDiv (Drug-like library)67400200.07
0.0791.08E-9SGTC_1961st076550 68.7 μMTimTec (Natural product derivative library)15793370.0679612
0.0782.20E-9SGTC_2525harmine 47.9 μMMiscellaneous52809530.0625ergosterol biosynthesis
0.0773.58E-9SGTC_1923st058458 19.1 μMTimTec (Natural product derivative library)35349820.0833333
0.0758.34E-9SGTC_2545solidagenone 89.6 μMMicrosource (Natural product library)67085720.0740741
0.0741.04E-8SGTC_9373122-0005 46.7 μMChemDiv (Drug-like library)7595350.0612245
0.0713.96E-8SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.077669960S ribosome export
0.0714.57E-8SGTC_2747doxorubicin 7.6 μMMiscellaneous317030.0846154anthracycline transcription coupled DNA repair
0.0672.74E-7SGTC_1970st070122 35.8 μMTimTec (Natural product derivative library)1029280.0833333TSC3-RPN4
0.0672.84E-7SGTC_7192810-4230 27.3 μMChemDiv (Drug-like library)7210790.0842105
0.0655.62E-7SGTC_32139129884 49.5 μMChembridge (Drug-like library)171907420.134615
0.0656.03E-7SGTC_610978-8327 98.6 μMChemDiv (Drug-like library)61500590.09375ergosterol biosynthesis
0.0648.86E-7SGTC_1972st070201 17.4 μMTimTec (Natural product derivative library)28290990.107843
0.0649.73E-7SGTC_8304076-0286 57.8 μMChemDiv (Drug-like library)68666590.101695translation
0.0631.08E-6SGTC_631k072-0230 41.5 μMChemDiv (Drug-like library)57731470.1360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1991st0730008.92 μM0.4431824301969TimTec (Natural product derivative library)466.57415.92105NEO1
SGTC_1797st04869556.8 μM0.4125592262TimTec (Natural product derivative library)352.266084.07403NEO1
SGTC_1638st00837060.6 μM0.40506324207134TimTec (Natural product derivative library)325.830363.46524ergosterol biosynthesis
SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride61.8 μM0.40506317037758TimTec (Natural product derivative library)323.857544.19413ergosterol biosynthesis
SGTC_1660st01296028.1 μM0.40506324207266TimTec (Natural product derivative library)580.4471550.052815iron homeostasis
SGTC_1629st00508544.9 μM0.33734924207091TimTec (Natural product derivative library)445.334994.49704RPP1 & pyrimidine depletion
SGTC_1657st01293943.4 μM0.24324324207257TimTec (Natural product derivative library)462.314515-2.478914
SGTC_687k029-004434.9 μM0.2127665829338ChemDiv (Drug-like library)337.2274.59214PDR1
SGTC_6721441-001853.7 μM0.2251141ChemDiv (Drug-like library)212.270323.19403
SGTC_1918oxolan-2-ylmethyl 3-bromobenzoate70.1 μM0.2551469TimTec (Natural product derivative library)285.133822.79203