4544-0043

1-[2-(2,4-dichlorophenoxy)ethyl]benzimidazol-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_197
Screen concentration 41.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 896523
SMILES C1=CC=C2C(=C1)N=C(N2CCOC3=C(C=C(C=C3)Cl)Cl)N
Standardized SMILES Nc1nc2ccccc2n1CCOc3ccc(Cl)cc3Cl
Molecular weight 322.1892
ALogP 4.31
H-bond donor count 1
H-bond acceptor count 3
Response signature endomembrane recycling

Pool Growth Kinetics
% growth inhibition (Het. pool) 42.02
% growth inhibition (Hom. pool) 11.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 896523
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.23||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:BRL1(YHR036W)|FD-Score:8.55|P-value:6.26E-18|Clearance:1.69||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC23(YHR166C)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC39(YCR093W)|FD-Score:6.86|P-value:3.40E-12|Clearance:0.17||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DED1(YOR204W)|FD-Score:3.18|P-value:7.39E-4|Clearance:0.07||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ERG11(YHR007C)|FD-Score:10.4|P-value:9.48E-26|Clearance:1.88||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.08||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:12.5|P-value:5.05E-36|Clearance:1.88||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GLC7(YER133W)|FD-Score:5.67|P-value:7.06E-9|Clearance:0.1||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KEI1(YDR367W)|FD-Score:3.11|P-value:9.35E-4|Clearance:0.04||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MED4(YOR174W)|FD-Score:3.85|P-value:5.86E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NEO1(YIL048W)|FD-Score:13.7|P-value:2.55E-43|Clearance:1.88||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP58(YOR310C)|FD-Score:5.86|P-value:2.31E-9|Clearance:0.19||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PDC2(YDR081C)|FD-Score:6.69|P-value:1.09E-11|Clearance:0.83||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PIK1(YNL267W)|FD-Score:12.9|P-value:1.22E-38|Clearance:1.88||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RFC1(YOR217W)|FD-Score:3.2|P-value:6.82E-4|Clearance:0.02||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPT1(YKL145W)|FD-Score:4.77|P-value:9.14E-7|Clearance:0.49||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSC58(YLR033W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:4.05|P-value:2.56E-5|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC4(YFL005W)|FD-Score:5.57|P-value:1.24E-8|Clearance:0.08||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SFH1(YLR321C)|FD-Score:3.85|P-value:5.96E-5|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SLD3(YGL113W)|FD-Score:5.49|P-value:2.01E-8|Clearance:0.23||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT14(YPL175W)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.2||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SPT15(YER148W)|FD-Score:4.92|P-value:4.32E-7|Clearance:0.15||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SPT5(YML010W)|FD-Score:-4.05|P-value:2.59E-5|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:TFA1(YKL028W)|FD-Score:5.04|P-value:2.38E-7|Clearance:0.12||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFG2(YGR005C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.03||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TIM54(YJL054W)|FD-Score:13.5|P-value:7.00E-42|Clearance:1.88||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOA1(YOR194C)|FD-Score:3.84|P-value:6.18E-5|Clearance:0.18||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:TOA2(YKL058W)|FD-Score:5.26|P-value:7.07E-8|Clearance:0.23||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TRR1(YDR353W)|FD-Score:4.29|P-value:9.13E-6|Clearance:0.07||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:YDR526C(YDR526C_d)|FD-Score:10.6|P-value:1.15E-26|Clearance:1.88||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR102W(YOR102W_d)|FD-Score:3.94|P-value:4.05E-5|Clearance:0.09||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:ACC1(YNR016C)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.23||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:BRL1(YHR036W)|FD-Score:8.55|P-value:6.26E-18|Clearance:1.69||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC23(YHR166C)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC39(YCR093W)|FD-Score:6.86|P-value:3.40E-12|Clearance:0.17||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DED1(YOR204W)|FD-Score:3.18|P-value:7.39E-4|Clearance:0.07||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ERG11(YHR007C)|FD-Score:10.4|P-value:9.48E-26|Clearance:1.88||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.08||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:12.5|P-value:5.05E-36|Clearance:1.88||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GLC7(YER133W)|FD-Score:5.67|P-value:7.06E-9|Clearance:0.1||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KEI1(YDR367W)|FD-Score:3.11|P-value:9.35E-4|Clearance:0.04||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MED4(YOR174W)|FD-Score:3.85|P-value:5.86E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NEO1(YIL048W)|FD-Score:13.7|P-value:2.55E-43|Clearance:1.88||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP58(YOR310C)|FD-Score:5.86|P-value:2.31E-9|Clearance:0.19||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PDC2(YDR081C)|FD-Score:6.69|P-value:1.09E-11|Clearance:0.83||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PIK1(YNL267W)|FD-Score:12.9|P-value:1.22E-38|Clearance:1.88||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RFC1(YOR217W)|FD-Score:3.2|P-value:6.82E-4|Clearance:0.02||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPT1(YKL145W)|FD-Score:4.77|P-value:9.14E-7|Clearance:0.49||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSC58(YLR033W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:4.05|P-value:2.56E-5|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC4(YFL005W)|FD-Score:5.57|P-value:1.24E-8|Clearance:0.08||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SFH1(YLR321C)|FD-Score:3.85|P-value:5.96E-5|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SLD3(YGL113W)|FD-Score:5.49|P-value:2.01E-8|Clearance:0.23||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT14(YPL175W)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.2||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SPT15(YER148W)|FD-Score:4.92|P-value:4.32E-7|Clearance:0.15||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SPT5(YML010W)|FD-Score:-4.05|P-value:2.59E-5|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:TFA1(YKL028W)|FD-Score:5.04|P-value:2.38E-7|Clearance:0.12||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFG2(YGR005C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.03||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TIM54(YJL054W)|FD-Score:13.5|P-value:7.00E-42|Clearance:1.88||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOA1(YOR194C)|FD-Score:3.84|P-value:6.18E-5|Clearance:0.18||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:TOA2(YKL058W)|FD-Score:5.26|P-value:7.07E-8|Clearance:0.23||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TRR1(YDR353W)|FD-Score:4.29|P-value:9.13E-6|Clearance:0.07||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:YDR526C(YDR526C_d)|FD-Score:10.6|P-value:1.15E-26|Clearance:1.88||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR102W(YOR102W_d)|FD-Score:3.94|P-value:4.05E-5|Clearance:0.09||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 896523
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AFT1(YGL071W)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APJ1(YNL077W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARF1(YDL192W)|FD-Score:6.86|P-value:3.46E-12||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ATG18(YFR021W)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATX1(YNL259C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BNI1(YNL271C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTS1(YPL069C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CCR4(YAL021C)|FD-Score:5.83|P-value:2.80E-9||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CHS7(YHR142W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLC1(YGR167W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COQ8(YGL119W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:CPR6(YLR216C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:5.78|P-value:3.71E-9||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAP1(YPL170W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DFG16(YOR030W)|FD-Score:4.57|P-value:2.41E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:ERG2(YMR202W)|FD-Score:14.5|P-value:1.14E-47||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:5.71|P-value:5.56E-9||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERV14(YGL054C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EST3(YIL009C-A)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FET3(YMR058W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:GEF1(YJR040W)|FD-Score:6.8|P-value:5.10E-12||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:HAL9(YOL089C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HBN1(YCL026C-B_p)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HHF1(YBR009C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HNT3(YOR258W)|FD-Score:3.83|P-value:6.34E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IMP2'(YIL154C)|FD-Score:11.7|P-value:9.06E-32||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LAA1(YJL207C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LEM3(YNL323W)|FD-Score:5.22|P-value:8.74E-8||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MDM20(YOL076W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MRPS16(YPL013C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS18(YPR134W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NEM1(YHR004C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NRP1(YDL167C)|FD-Score:5.33|P-value:4.95E-8||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NUR1(YDL089W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:NVJ2(YPR091C)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments Gene:PDR1(YGL013C)|FD-Score:8.68|P-value:1.98E-18||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFA3(YNL326C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PFK2(YMR205C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PFK26(YIL107C)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PHO80(YOL001W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PIM1(YBL022C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PKH3(YDR466W)|FD-Score:4.28|P-value:9.42E-6||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:POP2(YNR052C)|FD-Score:5.35|P-value:4.50E-8||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PSP2(YML017W)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PSY4(YBL046W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:PTC1(YDL006W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTR2(YKR093W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RCY1(YJL204C)|FD-Score:6.09|P-value:5.75E-10||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIM13(YMR154C)|FD-Score:5.46|P-value:2.41E-8||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:4.76|P-value:9.50E-7||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:5.01|P-value:2.72E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:ROX1(YPR065W)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RRT12(YCR045C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSC1(YGR056W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAC1(YKL212W)|FD-Score:8.7|P-value:1.66E-18||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCS2(YER120W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SMI1(YGR229C)|FD-Score:4.29|P-value:8.96E-6||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF8(YPL002C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNX3(YOR357C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SPT3(YDR392W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRT1(YMR101C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase Gene:SSZ1(YHR064C)|FD-Score:6.1|P-value:5.26E-10||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:TPN1(YGL186C)|FD-Score:9.42|P-value:2.26E-21||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRK1(YJL129C)|FD-Score:8.49|P-value:1.05E-17||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-3.79|P-value:7.58E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.85|P-value:5.96E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP18(YMR071C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:TVP23(YDR084C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VAC14(YLR386W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS21(YOR089C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS30(YPL120W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS35(YJL154C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS61(YDR136C_d)|FD-Score:-6.3|P-value:1.52E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:6.67|P-value:1.29E-11||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YDR186C(YDR186C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR271C(YDR271C_d)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YER034W(YER034W_p)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER119C-A(YER119C-A_d)|FD-Score:5.28|P-value:6.57E-8||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFL034W(YFL034W_p)|FD-Score:9.7|P-value:1.48E-22||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YGL199C(YGL199C_d)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR122W(YGR122W)|FD-Score:5.21|P-value:9.22E-8||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR130C(YGR130C)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YJR003C(YJR003C_p)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL070W(YKL070W_p)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL118W(YKL118W_d)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR111W(YLR111W_d)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Putative protein of unknown function Gene:YLR434C(YLR434C_d)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YLR456W(YLR456W_p)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YML007C-A(YML007C-A_p)|FD-Score:8.02|P-value:5.41E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR057C(YMR057C_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YNL324W(YNL324W_d)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:16.2|P-value:2.73E-59||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR092W(YPR092W_d)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZUO1(YGR285C)|FD-Score:16.2|P-value:1.53E-59||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:ADO1(YJR105W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AFT1(YGL071W)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APJ1(YNL077W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARF1(YDL192W)|FD-Score:6.86|P-value:3.46E-12||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ATG18(YFR021W)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATX1(YNL259C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BNI1(YNL271C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTS1(YPL069C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CCR4(YAL021C)|FD-Score:5.83|P-value:2.80E-9||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CHS7(YHR142W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLC1(YGR167W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COQ8(YGL119W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:CPR6(YLR216C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:5.78|P-value:3.71E-9||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAP1(YPL170W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DFG16(YOR030W)|FD-Score:4.57|P-value:2.41E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:ERG2(YMR202W)|FD-Score:14.5|P-value:1.14E-47||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:5.71|P-value:5.56E-9||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERV14(YGL054C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EST3(YIL009C-A)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FET3(YMR058W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:GEF1(YJR040W)|FD-Score:6.8|P-value:5.10E-12||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:HAL9(YOL089C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HBN1(YCL026C-B_p)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HHF1(YBR009C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HNT3(YOR258W)|FD-Score:3.83|P-value:6.34E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IMP2'(YIL154C)|FD-Score:11.7|P-value:9.06E-32||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LAA1(YJL207C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LEM3(YNL323W)|FD-Score:5.22|P-value:8.74E-8||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MDM20(YOL076W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MRPS16(YPL013C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS18(YPR134W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NEM1(YHR004C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NRP1(YDL167C)|FD-Score:5.33|P-value:4.95E-8||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NUR1(YDL089W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:NVJ2(YPR091C)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments Gene:PDR1(YGL013C)|FD-Score:8.68|P-value:1.98E-18||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFA3(YNL326C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PFK2(YMR205C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PFK26(YIL107C)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PHO80(YOL001W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PIM1(YBL022C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PKH3(YDR466W)|FD-Score:4.28|P-value:9.42E-6||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:POP2(YNR052C)|FD-Score:5.35|P-value:4.50E-8||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PSP2(YML017W)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PSY4(YBL046W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:PTC1(YDL006W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTR2(YKR093W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RCY1(YJL204C)|FD-Score:6.09|P-value:5.75E-10||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIM13(YMR154C)|FD-Score:5.46|P-value:2.41E-8||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:4.76|P-value:9.50E-7||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:5.01|P-value:2.72E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:ROX1(YPR065W)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RRT12(YCR045C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RSC1(YGR056W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAC1(YKL212W)|FD-Score:8.7|P-value:1.66E-18||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCS2(YER120W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SMI1(YGR229C)|FD-Score:4.29|P-value:8.96E-6||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF8(YPL002C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNX3(YOR357C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SPT3(YDR392W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRT1(YMR101C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase Gene:SSZ1(YHR064C)|FD-Score:6.1|P-value:5.26E-10||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:TPN1(YGL186C)|FD-Score:9.42|P-value:2.26E-21||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRK1(YJL129C)|FD-Score:8.49|P-value:1.05E-17||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-3.79|P-value:7.58E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.85|P-value:5.96E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP18(YMR071C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:TVP23(YDR084C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VAC14(YLR386W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS21(YOR089C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS30(YPL120W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS35(YJL154C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS61(YDR136C_d)|FD-Score:-6.3|P-value:1.52E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:6.67|P-value:1.29E-11||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YDR186C(YDR186C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR271C(YDR271C_d)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YER034W(YER034W_p)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER119C-A(YER119C-A_d)|FD-Score:5.28|P-value:6.57E-8||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFL034W(YFL034W_p)|FD-Score:9.7|P-value:1.48E-22||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YGL199C(YGL199C_d)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR122W(YGR122W)|FD-Score:5.21|P-value:9.22E-8||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR130C(YGR130C)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YJR003C(YJR003C_p)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL070W(YKL070W_p)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL118W(YKL118W_d)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR111W(YLR111W_d)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Putative protein of unknown function Gene:YLR434C(YLR434C_d)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YLR456W(YLR456W_p)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YML007C-A(YML007C-A_p)|FD-Score:8.02|P-value:5.41E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR057C(YMR057C_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YNL324W(YNL324W_d)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:16.2|P-value:2.73E-59||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR092W(YPR092W_d)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZUO1(YGR285C)|FD-Score:16.2|P-value:1.53E-59||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W13.702.55E-431.88NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YJL054W13.507.00E-421.88TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
YNL267W12.901.22E-381.88PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YMR134W_p12.505.05E-361.88ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YDR526C_d10.601.15E-261.88YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHR007C10.409.48E-261.88ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YHR036W8.556.26E-181.69BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YCR093W6.863.40E-120.17CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YDR081C6.691.09E-110.83PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YOR310C5.862.31E-90.19NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YER133W5.677.06E-90.10GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YFL005W5.571.24E-80.08SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YGL113W5.492.01E-80.23SLD3Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
YKL058W5.267.07E-80.23TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YKL028W5.042.38E-70.12TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR285C16.201.53E-59ZUO1Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
YKL126W16.202.73E-59YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YMR202W14.501.14E-47ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YIL154C11.709.06E-32IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YFL034W_p9.701.48E-22YFL034W_pPutative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
YGL186C9.422.26E-21TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YKL212W8.701.66E-18SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YGL013C8.681.98E-18PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YJL129C8.491.05E-17TRK1Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
YML007C-A_p8.025.41E-16YML007C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
YDL192W6.863.46E-12ARF1ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
YJR040W6.805.10E-12GEF1Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
YDR372C6.671.29E-11VPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
YHR064C6.105.26E-10SSZ1Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
YJL204C6.095.75E-10RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth

GO enrichment analysis for SGTC_197
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3774.70E-199SGTC_12300170-0365 3.8 μMChemDiv (Drug-like library)40189320.15942endomembrane recycling
0.3701.59E-190SGTC_22577960413 200.0 μMChembridge (Fragment library)29746890.172414NEO1-PIK1
0.3645.48E-184SGTC_6491123-0311 367.0 μMChemDiv (Drug-like library)6936290.117647endomembrane recycling
0.3544.34E-174SGTC_20365160441 105.0 μMChembridge (Fragment library)1345340.131148NEO1
0.3502.05E-169SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.12987NEO1-PIK1
0.3456.76E-165SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.194444ERG2
0.3369.72E-156SGTC_7881182-0090 188.0 μMChemDiv (Drug-like library)57151850.0615385endomembrane recycling
0.3344.76E-154SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.103896ERG2
0.3151.81E-136SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.144737calcium & mitochondrial duress
0.3112.41E-132SGTC_1801st050089 28.4 μMTimTec (Natural product derivative library)50084930.131579NEO1-PIK1
0.3111.82E-132SGTC_1754st042065 16.1 μMTimTec (Natural product derivative library)11262360.0869565NEO1-PIK1
0.3042.60E-126SGTC_1463448-8650 53.1 μMChemDiv (Drug-like library)28914880.0568182NEO1-PIK1
0.2992.40E-122SGTC_20214019055 173.0 μMChembridge (Fragment library)448277080.0952381endomembrane recycling
0.2934.44E-117SGTC_1803st050068 52.4 μMTimTec (Natural product derivative library)39840590.103896NEO1-PIK1
0.2905.80E-115SGTC_3133474-0040 5.0 μMChemDiv (Drug-like library)10433750.0735294NEO1-PIK1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_15120259-054412.2 μM0.44444418476ChemDiv (Drug-like library)293.1484.43412
SGTC_21495554786194.83 μM0.442308745063Chembridge (Fragment library)230.308821.94613cell wall signaling
SGTC_2987901833871.43 μM0.4406785055682Chembridge (Drug-like library)330.808663.98313RPP1 & pyrimidine depletion
SGTC_10462001-0062216 μM0.396825739778ChemDiv (Drug-like library)341.427363.60714
SGTC_2862902763471.43 μM0.3968255012562Chembridge (Drug-like library)324.416764.45213ERG2
SGTC_3295912236253.57 μM0.3754730473Chembridge (Drug-like library)349.214564.14614
SGTC_7243910-0327137 μM0.3606562885483ChemDiv (Drug-like library)316.782083.59813ERG2
SGTC_14133910-053534.4 μM0.3432842876951ChemDiv (Drug-like library)358.861825.21313
SGTC_6681682-049962.6 μM0.333333682214ChemDiv (Drug-like library)324.20184.10612
SGTC_13702001-009244.6 μM0.333333790957ChemDiv (Drug-like library)256.2749233.38903