st070275

3-[2-(aziridin-1-yl)ethyl]-5-phenoxy-1H-indole hydrochloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1973
Screen concentration 50.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 17118334
SMILES C1CN1CCC2=CNC3=C2C=C(C=C3)OC4=CC=CC=C4.Cl
Standardized SMILES C(Cc1c[nH]c2ccc(Oc3ccccc3)cc12)N4CC4
Molecular weight 314.8093
ALogP 4.28
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.11
% growth inhibition (Hom. pool) 6.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17118334
Download HIP data (tab-delimited text)  (excel)
Gene:GPI2(YPL076W)|FD-Score:-3.39|P-value:3.50E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:RLP7(YNL002C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.18||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:SRP101(YDR292C)|FD-Score:3.98|P-value:3.38E-5|Clearance:0.45||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:TAF12(YDR145W)|FD-Score:3.35|P-value:4.07E-4|Clearance:0.08||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF9(YMR236W)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:YJR023C(YJR023C_d)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.19||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:GPI2(YPL076W)|FD-Score:-3.39|P-value:3.50E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:RLP7(YNL002C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.18||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:SRP101(YDR292C)|FD-Score:3.98|P-value:3.38E-5|Clearance:0.45||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:TAF12(YDR145W)|FD-Score:3.35|P-value:4.07E-4|Clearance:0.08||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF9(YMR236W)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:YJR023C(YJR023C_d)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.19||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17118334
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ARO2(YGL148W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG20(YDL113C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:ATP25(YMR098C)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BNA1(YJR025C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BUD27(YFL023W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:DAL81(YIR023W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ECM25(YJL201W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:ERG2(YMR202W)|FD-Score:6.45|P-value:5.44E-11||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FLC3(YGL139W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GCN20(YFR009W)|FD-Score:-5.17|P-value:1.16E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GPB2(YAL056W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:HTZ1(YOL012C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:KRE2(YDR483W)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:KTR6(YPL053C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MSG5(YNL053W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSH6(YDR097C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:PMP2(YEL017C-A)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PRC1(YMR297W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRO2(YOR323C)|FD-Score:9.51|P-value:9.46E-22||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTK2(YJR059W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:QCR7(YDR529C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RGP1(YDR137W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL7B(YPL198W)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SOL4(YGR248W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SRL1(YOR247W)|FD-Score:-3.85|P-value:5.82E-5||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SWA2(YDR320C)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWC5(YBR231C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TCB3(YML072C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TLG2(YOL018C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:UNG1(YML021C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VPS1(YKR001C)|FD-Score:-3.76|P-value:8.47E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:-4.28|P-value:9.50E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAF9(YNL107W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBL071C(YBL071C_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR016W(YBR016W)|FD-Score:4.75|P-value:9.99E-7||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR141C(YBR141C_p)|FD-Score:4.34|P-value:7.08E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YDL068W(YDL068W_d)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR431W(YDR431W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR521W(YDR521W_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YHB1(YGR234W)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YHL042W(YHL042W_p)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YHR139C-A(YHR139C-A_d)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR087W(YJR087W_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Gene:YKU80(YMR106C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR257W(YLR257W_p)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YPK1(YKL126W)|FD-Score:11|P-value:1.39E-28||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR078C(YPR078C_p)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:ACB1(YGR037C)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ARO2(YGL148W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG20(YDL113C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:ATP25(YMR098C)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BNA1(YJR025C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BUD27(YFL023W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:DAL81(YIR023W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ECM25(YJL201W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:ERG2(YMR202W)|FD-Score:6.45|P-value:5.44E-11||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FLC3(YGL139W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GCN20(YFR009W)|FD-Score:-5.17|P-value:1.16E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GPB2(YAL056W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:HTZ1(YOL012C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:KRE2(YDR483W)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:KTR6(YPL053C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MSG5(YNL053W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSH6(YDR097C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:PMP2(YEL017C-A)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PRC1(YMR297W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRO2(YOR323C)|FD-Score:9.51|P-value:9.46E-22||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTK2(YJR059W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:QCR7(YDR529C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RGP1(YDR137W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL7B(YPL198W)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SOL4(YGR248W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SRL1(YOR247W)|FD-Score:-3.85|P-value:5.82E-5||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SWA2(YDR320C)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWC5(YBR231C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TCB3(YML072C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TLG2(YOL018C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:UNG1(YML021C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VPS1(YKR001C)|FD-Score:-3.76|P-value:8.47E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:-4.28|P-value:9.50E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAF9(YNL107W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBL071C(YBL071C_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR016W(YBR016W)|FD-Score:4.75|P-value:9.99E-7||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR141C(YBR141C_p)|FD-Score:4.34|P-value:7.08E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YDL068W(YDL068W_d)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR431W(YDR431W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR521W(YDR521W_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YHB1(YGR234W)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YHL042W(YHL042W_p)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YHR139C-A(YHR139C-A_d)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR087W(YJR087W_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Gene:YKU80(YMR106C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR257W(YLR257W_p)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YPK1(YKL126W)|FD-Score:11|P-value:1.39E-28||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR078C(YPR078C_p)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR292C3.983.38E-50.45SRP101Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
YJR023C_d3.542.01E-40.19YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YDR145W3.354.07E-40.08TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YNL002C3.275.44E-40.18RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YKL122C3.090.001000.02SRP21Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
YFL008W3.070.001050.07SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YOR169C_d3.010.001320.03YOR169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W
YER146W2.980.001450.04LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YFR051C2.930.001680.04RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YDL217C2.900.001880.08TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YBL073W_d2.810.002450.07YBL073W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C
YKL058W2.740.003040.04TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YOR207C2.700.003450.00RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YPR187W2.700.003500.14RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YGR251W2.560.005200.00NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W11.001.39E-28YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YOR323C9.519.46E-22PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YMR202W6.455.44E-11ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YIR023W5.081.92E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YBR016W4.759.99E-7YBR016WTail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
YDR483W4.395.62E-6KRE2Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
YBR141C_p4.347.08E-6YBR141C_pPutative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
YHR139C-A_d4.082.27E-5YHR139C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPR078C_p3.973.66E-5YPR078C_pPutative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible
YFL023W3.875.48E-5BUD27Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP
YGL139W3.807.16E-5FLC3Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER
YGR234W3.768.47E-5YHB1Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YNL053W3.749.06E-5MSG5Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p
YGR037C3.739.47E-5ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YPL198W3.739.60E-5RPL7BRibosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1973
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2871.57E-112SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.125ERG2
0.2601.79E-91SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.0581395
0.2508.28E-85SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.135135ERG2
0.2474.46E-83SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.238806ERG2
0.2461.81E-82SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.135802
0.2263.63E-69SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.164384calcium & mitochondrial duress
0.2231.41E-67SGTC_23749071832 200.0 μMChembridge (Fragment library)173816980.1endomembrane recycling
0.2192.84E-65SGTC_2732terfenadine 20.0 μMMiscellaneous54050.183099ERG2
0.2183.70E-64SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.173333ERG2
0.2148.34E-62SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.130952
0.2049.56E-57SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.111111calcium & mitochondrial duress
0.2011.10E-54SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.0540541ERG2
0.1942.09E-51SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0757576ERG2
0.1799.58E-44SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0833333ERG2
0.1791.07E-43SGTC_20464110841 106.0 μMChembridge (Fragment library)441196300.169811iron homeostasis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20515263410176 μM0.392157227584Chembridge (Fragment library)229.320762.15412
SGTC_10995-nonyloxytryptamine2.85 μM0.38596523581825NIH Clinical Collection338.915245.52532
SGTC_1754st04206516.14 μM0.3773581126236TimTec (Natural product derivative library)355.47544.64612NEO1-PIK1
SGTC_9453381-076811.3 μM0.3773581126236ChemDiv (Drug-like library)355.47544.64612endomembrane recycling
SGTC_1818st05213656.3 μM0.368421674169TimTec (Natural product derivative library)339.861784.40212NEO1-PIK1
SGTC_1825st05214362.6 μM0.363636674173TimTec (Natural product derivative library)319.44334.22412
SGTC_540159-00559 μM0.3559323319099ChemDiv (Drug-like library)530.476949.51910ERG2
SGTC_12630774-120864.8 μM0.345455906585ChemDiv (Drug-like library)304.342543.51612
SGTC_60844-001319.84 μM0.3392861584900ChemDiv (Drug-like library)451.580888.7710
SGTC_452diindolylmethane8 μM0.3333333071ICCB bioactive library246.306464.39120RNA processing & uracil transport