st069448

(3Z)-3-hydroxyimino-10,13-dimethyl-1,2,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-ol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1974
Screen concentration 26.9 μM
Source TimTec (Natural product derivative library)
PubChem CID 6323587
SMILES CC12CCC(=NO)CC1CCC3C2CCC4(C3CCC4O)C
Standardized SMILES CC12CCC3C(CCC4CC(=NO)CCC34C)C1CCC2O
Molecular weight 305.4549
ALogP 3.37
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.73
% growth inhibition (Hom. pool) 10.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6323587
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:22|P-value:9.49E-108|Clearance:17.08||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CCT7(YJL111W)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC39(YCR093W)|FD-Score:4.63|P-value:1.81E-6|Clearance:0.5||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:ERG11(YHR007C)|FD-Score:4.13|P-value:1.80E-5|Clearance:0.46||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ESA1(YOR244W)|FD-Score:-3.1|P-value:9.60E-4|Clearance:0||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:GAB1(YLR459W)|FD-Score:-3.18|P-value:7.47E-4|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GPN2(YOR262W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.61||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:MEX67(YPL169C)|FD-Score:4.94|P-value:3.85E-7|Clearance:0.31||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MIM1(YOL026C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.06||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:RPB7(YDR404C)|FD-Score:-3.23|P-value:6.10E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPO26(YPR187W)|FD-Score:-3.81|P-value:7.06E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:-4.64|P-value:1.73E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:-3.44|P-value:2.95E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SPB1(YCL054W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:YDR526C(YDR526C_d)|FD-Score:-3.11|P-value:9.47E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACC1(YNR016C)|FD-Score:22|P-value:9.49E-108|Clearance:17.08||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:CCT7(YJL111W)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC39(YCR093W)|FD-Score:4.63|P-value:1.81E-6|Clearance:0.5||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:ERG11(YHR007C)|FD-Score:4.13|P-value:1.80E-5|Clearance:0.46||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ESA1(YOR244W)|FD-Score:-3.1|P-value:9.60E-4|Clearance:0||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:GAB1(YLR459W)|FD-Score:-3.18|P-value:7.47E-4|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GPN2(YOR262W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.61||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:MEX67(YPL169C)|FD-Score:4.94|P-value:3.85E-7|Clearance:0.31||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MIM1(YOL026C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.06||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:RPB7(YDR404C)|FD-Score:-3.23|P-value:6.10E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPO26(YPR187W)|FD-Score:-3.81|P-value:7.06E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:-4.64|P-value:1.73E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:-3.44|P-value:2.95E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SPB1(YCL054W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:YDR526C(YDR526C_d)|FD-Score:-3.11|P-value:9.47E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6323587
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ADD37(YMR184W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:APL6(YGR261C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:BRR1(YPR057W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CST6(YIL036W)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DOG2(YHR043C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DSE3(YOR264W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG2(YBR281C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:DUG3(YNL191W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ECM25(YJL201W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:EGD1(YPL037C)|FD-Score:-3.75|P-value:8.82E-5||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ENV9(YOR246C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FEN1(YCR034W)|FD-Score:4.99|P-value:3.01E-7||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FUR4(YBR021W)|FD-Score:5.48|P-value:2.18E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GET3(YDL100C)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GTS1(YGL181W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:GYP1(YOR070C)|FD-Score:-3.93|P-value:4.31E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:GZF3(YJL110C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HFD1(YMR110C)|FD-Score:6.47|P-value:4.75E-11||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:HXT5(YHR096C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication Gene:JIP3(YLR331C_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:LIA1(YJR070C)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LPD1(YFL018C)|FD-Score:-3.88|P-value:5.23E-5||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:LSM7(YNL147W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDM1(YML104C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MFA2(YNL145W)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MKK1(YOR231W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:NAT1(YDL040C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:PBS2(YJL128C)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PFK26(YIL107C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PHO89(YBR296C)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PSP2(YML017W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PSR2(YLR019W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PTR2(YKR093W)|FD-Score:4.78|P-value:8.77E-7||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAD33(YML011C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAM1(YDL090C)|FD-Score:5.79|P-value:3.54E-9||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RAS2(YNL098C)|FD-Score:4.86|P-value:5.86E-7||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:REG1(YDR028C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:ROX1(YPR065W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL6B(YLR448W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPN13(YLR421C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:SPT3(YDR392W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT4(YGR063C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUB1(YMR039C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THP1(YOL072W)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TMA108(YIL137C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TMA7(YLR262C-A)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TOM7(YNL070W)|FD-Score:6.09|P-value:5.72E-10||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPK1(YJL164C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRI1(YMR233W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:5.02|P-value:2.56E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS51(YKR020W)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YBR016W(YBR016W)|FD-Score:4.79|P-value:8.52E-7||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR027C(YBR027C_d)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR099C(YBR099C_d)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YGL042C(YGL042C_d)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YIL029C(YIL029C_p)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL077C(YIL077C_p)|FD-Score:5.76|P-value:4.20E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YIM1(YMR152W)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YKL169C(YKL169C_d)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR194C(YLR194C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YNL092W(YNL092W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNL211C(YNL211C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YPL062W(YPL062W_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR146C(YPR146C_d)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AAC3(YBR085W)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ADD37(YMR184W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:APL6(YGR261C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:BRR1(YPR057W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CST6(YIL036W)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DOG2(YHR043C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DSE3(YOR264W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG2(YBR281C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:DUG3(YNL191W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ECM25(YJL201W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:EGD1(YPL037C)|FD-Score:-3.75|P-value:8.82E-5||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ENV9(YOR246C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FEN1(YCR034W)|FD-Score:4.99|P-value:3.01E-7||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FUR4(YBR021W)|FD-Score:5.48|P-value:2.18E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GET3(YDL100C)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GTS1(YGL181W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:GYP1(YOR070C)|FD-Score:-3.93|P-value:4.31E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:GZF3(YJL110C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HFD1(YMR110C)|FD-Score:6.47|P-value:4.75E-11||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:HXT5(YHR096C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication Gene:JIP3(YLR331C_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:LIA1(YJR070C)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LPD1(YFL018C)|FD-Score:-3.88|P-value:5.23E-5||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:LSM7(YNL147W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDM1(YML104C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MFA2(YNL145W)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MKK1(YOR231W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:NAT1(YDL040C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:PBS2(YJL128C)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PFK26(YIL107C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PHO89(YBR296C)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PSP2(YML017W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PSR2(YLR019W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PTR2(YKR093W)|FD-Score:4.78|P-value:8.77E-7||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAD33(YML011C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAM1(YDL090C)|FD-Score:5.79|P-value:3.54E-9||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RAS2(YNL098C)|FD-Score:4.86|P-value:5.86E-7||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:REG1(YDR028C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:ROX1(YPR065W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL6B(YLR448W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPN13(YLR421C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:SPT3(YDR392W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT4(YGR063C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUB1(YMR039C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THP1(YOL072W)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TMA108(YIL137C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TMA7(YLR262C-A)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TOM7(YNL070W)|FD-Score:6.09|P-value:5.72E-10||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPK1(YJL164C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRI1(YMR233W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:5.02|P-value:2.56E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS51(YKR020W)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YBR016W(YBR016W)|FD-Score:4.79|P-value:8.52E-7||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR027C(YBR027C_d)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR099C(YBR099C_d)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YGL042C(YGL042C_d)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YIL029C(YIL029C_p)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL077C(YIL077C_p)|FD-Score:5.76|P-value:4.20E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YIM1(YMR152W)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YKL169C(YKL169C_d)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR194C(YLR194C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YNL092W(YNL092W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNL211C(YNL211C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YPL062W(YPL062W_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR146C(YPR146C_d)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YNR016C22.009.49E-10817.10ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YPL169C4.943.85E-70.31MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YCR093W4.631.81E-60.50CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YHR007C4.131.80E-50.47ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YOL026C3.671.23E-40.06MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YOR262W3.611.55E-40.61GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YER146W3.000.001370.07LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YMR298W2.930.001690.03LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
YJR013W2.900.001860.13GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YIL061C2.770.002830.02SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YBL018C2.750.002970.12POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR153C2.630.004210.07ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YIL046W2.560.005170.04MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YLR249W2.530.005770.03YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YFR052W2.500.006230.00RPN12Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR110C6.474.75E-11HFD1Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
YNL070W6.095.72E-10TOM7Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
YDL090C5.793.54E-9RAM1Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
YIL077C_p5.764.20E-9YIL077C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YBR021W5.482.18E-8FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YBR296C5.171.20E-7PHO89Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
YIL029C_p5.032.44E-7YIL029C_pPutative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W
YBR058C-A5.022.56E-7TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YCR034W4.993.01E-7FEN1Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication
YJR070C4.983.18E-7LIA1Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
YNL098C4.865.86E-7RAS2GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
YBR016W4.798.52E-7YBR016WTail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
YKR093W4.788.77E-7PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YJL164C4.701.28E-6TPK1cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
YNL145W4.661.56E-6MFA2Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1

GO enrichment analysis for SGTC_1974
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1512.16E-31SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0547945RSC complex & mRNA processing
0.1498.31E-31SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0151515TSC3-RPN4
0.1454.75E-29SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0454545
0.1331.19E-24SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.0394737RSC complex & mRNA processing
0.1195.37E-20SGTC_606k015-0008 38.6 μMChemDiv (Drug-like library)19704750.0147059
0.1164.97E-19SGTC_1586deoxycorticosterone acetate 53.7 μMTimTec (Pure natural product library)59520.239437
0.1155.71E-19SGTC_2248tofa 1.1 μMMiscellaneous1151750.0441176sphingolipid biosynthesis & PDR1
0.1071.58E-16SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.0540541RSC complex & mRNA processing
0.1071.73E-16SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.0266667amide catabolism
0.1063.23E-16SGTC_2747doxorubicin 7.6 μMMiscellaneous317030.0679612anthracycline transcription coupled DNA repair
0.1055.44E-16SGTC_2408sulbentine 18.4 μMMiscellaneous676860.030303
0.1023.97E-15SGTC_15846-fluoro-dl-tryptophan 90.0 μMTimTec (Pure natural product library)949370.0273973mitochondrial processes
0.1016.88E-15SGTC_1811447-1644 8.1 μMChemDiv (Drug-like library)5346000.0609756endomembrane recycling
0.1001.13E-14SGTC_8710574-0004 149.0 μMChemDiv (Drug-like library)95511730.0263158
0.0992.66E-14SGTC_15956-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 67.5 μMTimTec (Pure natural product library)6887880.0394737

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_25005-alpha-androstan-3,17-dione61.44 μM0.4230776710655Microsource (Natural product library)288.424423.40202
SGTC_1572epiandrosterone68.9 μM0.407407441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.407407441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.4074072754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_1750st0818592.36 μM0.385291695TimTec (Natural product derivative library)275.472024.43511endomembrane recycling
SGTC_231b-estradiol524 μM0.368421450Miscellaneous272.381963.83822
SGTC_1560lithocholic acid53.1 μM0.3333339903TimTec (Pure natural product library)376.57265.18423
SGTC_417st075190100 μM0.3222757TimTec (Pure natural product library)376.488025.51213
SGTC_1517deoxycholic acid methyl ester49.2 μM0.28169229346TimTec (Pure natural product library)406.598584.30724
SGTC_2531methyl deoxycholate70.07 μM0.2816916667744Microsource (Natural product library)406.598584.30724
SGTC_2564betulinic acid100 μM0.2463776708517Microsource (Natural product library)442.673746.3423