st071826

propan-2-yl 2-amino-6-(hydroxymethyl)-8-oxo-4-[2-(trifluoromethyl)phenyl]-4H-pyrano[3,2-b]pyran-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1981
Screen concentration 47.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 11838600
SMILES CC(C)OC(=O)C1=C(OC2=C(C1C3=CC=CC=C3C(F)(F)F)OC(=CC2=O)CO)N
Standardized SMILES CC(C)OC(=O)C1=C(N)OC2=C(OC(=CC2=O)CO)C1c3ccccc3C(F)(F)F
Molecular weight 425.3552
ALogP 1.97
H-bond donor count 2
H-bond acceptor count 10
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.78
% growth inhibition (Hom. pool) -1.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 11838600
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:3.85|P-value:6.01E-5|Clearance:0.1||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CCT7(YJL111W)|FD-Score:4.39|P-value:5.64E-6|Clearance:0.27||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DPS1(YLL018C)|FD-Score:3.74|P-value:9.03E-5|Clearance:0.24||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:DYS1(YHR068W)|FD-Score:4.12|P-value:1.90E-5|Clearance:0.06||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERD2(YBL040C)|FD-Score:-3.88|P-value:5.13E-5|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG27(YLR100W)|FD-Score:-3.23|P-value:6.20E-4|Clearance:0||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:ILS1(YBL076C)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.02||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MCM5(YLR274W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.17||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MTR3(YGR158C)|FD-Score:-4.1|P-value:2.06E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NAT2(YGR147C)|FD-Score:3.23|P-value:6.24E-4|Clearance:0.03||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NUS1(YDL193W)|FD-Score:3.33|P-value:4.39E-4|Clearance:0.1||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:ORC2(YBR060C)|FD-Score:-3.35|P-value:4.01E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:RIX1(YHR197W)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.1||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RTP1(YMR185W_p)|FD-Score:4.06|P-value:2.41E-5|Clearance:0.17||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SUA7(YPR086W)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.05||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:UBA2(YDR390C)|FD-Score:3.2|P-value:6.90E-4|Clearance:0.01||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:UTP8(YGR128C)|FD-Score:4.48|P-value:3.81E-6|Clearance:0.27||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:BMS1(YPL217C)|FD-Score:3.85|P-value:6.01E-5|Clearance:0.1||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CCT7(YJL111W)|FD-Score:4.39|P-value:5.64E-6|Clearance:0.27||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DPS1(YLL018C)|FD-Score:3.74|P-value:9.03E-5|Clearance:0.24||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:DYS1(YHR068W)|FD-Score:4.12|P-value:1.90E-5|Clearance:0.06||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERD2(YBL040C)|FD-Score:-3.88|P-value:5.13E-5|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG27(YLR100W)|FD-Score:-3.23|P-value:6.20E-4|Clearance:0||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:ILS1(YBL076C)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.02||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MCM5(YLR274W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.17||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MTR3(YGR158C)|FD-Score:-4.1|P-value:2.06E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NAT2(YGR147C)|FD-Score:3.23|P-value:6.24E-4|Clearance:0.03||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NUS1(YDL193W)|FD-Score:3.33|P-value:4.39E-4|Clearance:0.1||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:ORC2(YBR060C)|FD-Score:-3.35|P-value:4.01E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:RIX1(YHR197W)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.1||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RTP1(YMR185W_p)|FD-Score:4.06|P-value:2.41E-5|Clearance:0.17||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SUA7(YPR086W)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.05||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:UBA2(YDR390C)|FD-Score:3.2|P-value:6.90E-4|Clearance:0.01||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:UTP8(YGR128C)|FD-Score:4.48|P-value:3.81E-6|Clearance:0.27||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 11838600
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ADI1(YMR009W)|FD-Score:-3.93|P-value:4.30E-5||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:AGC1(YPR021C)|FD-Score:-4.46|P-value:4.14E-6||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:APL5(YPL195W)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APS3(YJL024C)|FD-Score:-5.08|P-value:1.86E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:AYR1(YIL124W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BIO2(YGR286C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:CAX4(YGR036C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation Gene:CDC73(YLR418C)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB2(YPR119W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COX23(YHR116W)|FD-Score:-3.95|P-value:3.93E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CYS3(YAL012W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:ERI1(YPL096C-A)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:ERV14(YGL054C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FCY2(YER056C)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:GEP4(YHR100C)|FD-Score:-4.03|P-value:2.85E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GOS1(YHL031C)|FD-Score:4.41|P-value:5.29E-6||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPD1(YDL022W)|FD-Score:-4.09|P-value:2.15E-5||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:HAL9(YOL089C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:LEO1(YOR123C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:NPT1(YOR209C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:PER1(YCR044C)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX27(YOR193W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PMR1(YGL167C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POP2(YNR052C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRS1(YKL181W)|FD-Score:5.73|P-value:5.16E-9||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK1(YKL198C)|FD-Score:-4.03|P-value:2.77E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RPL18B(YNL301C)|FD-Score:-5.02|P-value:2.57E-7||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:SFP1(YLR403W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLC1(YDL052C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SRP40(YKR092C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSA3(YBL075C)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TIF4631(YGR162W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TNA1(YGR260W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:VAC14(YLR386W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VBA4(YDR119W_p)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VMA22(YHR060W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VMA8(YEL051W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR182C-A(YBR182C-A_p)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR284W(YBR284W_p)|FD-Score:4.29|P-value:8.92E-6||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCL049C(YCL049C_p)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YGK3(YOL128C)|FD-Score:-4.55|P-value:2.74E-6||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL072C(YGL072C_d)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL242C(YGL242C_p)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YKL118W(YKL118W_d)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR434C(YLR434C_d)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML009C-A(YML009C-A_d)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:5.72|P-value:5.25E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YPL257W(YPL257W_p)|FD-Score:3.72|P-value:9.89E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:AAT2(YLR027C)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ADI1(YMR009W)|FD-Score:-3.93|P-value:4.30E-5||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:AGC1(YPR021C)|FD-Score:-4.46|P-value:4.14E-6||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:APL5(YPL195W)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APS3(YJL024C)|FD-Score:-5.08|P-value:1.86E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:AYR1(YIL124W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BIO2(YGR286C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:CAX4(YGR036C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation Gene:CDC73(YLR418C)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB2(YPR119W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COX23(YHR116W)|FD-Score:-3.95|P-value:3.93E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CYS3(YAL012W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:ERI1(YPL096C-A)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:ERV14(YGL054C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FCY2(YER056C)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:GEP4(YHR100C)|FD-Score:-4.03|P-value:2.85E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GOS1(YHL031C)|FD-Score:4.41|P-value:5.29E-6||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPD1(YDL022W)|FD-Score:-4.09|P-value:2.15E-5||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:HAL9(YOL089C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:LEO1(YOR123C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:NPT1(YOR209C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:PER1(YCR044C)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX27(YOR193W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PMR1(YGL167C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POP2(YNR052C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRS1(YKL181W)|FD-Score:5.73|P-value:5.16E-9||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK1(YKL198C)|FD-Score:-4.03|P-value:2.77E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RPL18B(YNL301C)|FD-Score:-5.02|P-value:2.57E-7||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:SFP1(YLR403W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLC1(YDL052C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SRP40(YKR092C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSA3(YBL075C)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TIF4631(YGR162W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TNA1(YGR260W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:VAC14(YLR386W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VBA4(YDR119W_p)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VMA22(YHR060W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VMA8(YEL051W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR182C-A(YBR182C-A_p)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR284W(YBR284W_p)|FD-Score:4.29|P-value:8.92E-6||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCL049C(YCL049C_p)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YGK3(YOL128C)|FD-Score:-4.55|P-value:2.74E-6||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL072C(YGL072C_d)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL242C(YGL242C_p)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YKL118W(YKL118W_d)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR434C(YLR434C_d)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML009C-A(YML009C-A_d)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:5.72|P-value:5.25E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YPL257W(YPL257W_p)|FD-Score:3.72|P-value:9.89E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR128C4.483.81E-60.27UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YJL111W4.395.64E-60.27CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YHR068W4.121.90E-50.06DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YMR185W_p4.062.41E-50.17RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YPR086W3.894.98E-50.05SUA7Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
YPL217C3.856.01E-50.10BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YLL018C3.749.03E-50.24DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YLR274W3.502.31E-40.17MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YDL193W3.334.39E-40.10NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YGR147C3.236.24E-40.03NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YDR390C3.206.90E-40.01UBA2Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YBL076C3.197.21E-40.02ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YHR197W3.167.83E-40.10RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YBR121C3.070.001080.17GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YPR142C_d2.900.001880.03YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL181W5.735.16E-9PRS15-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
YML094C-A_d5.725.25E-9YML094C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
YLR418C5.286.49E-8CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YPL195W5.191.06E-7APL5Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
YKL118W_d4.973.33E-7YKL118W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
YGL072C_d4.721.20E-6YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YGL242C_p4.572.46E-6YGL242C_pPutative protein of unknown function; deletion mutant is viable
YBL075C4.415.24E-6SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
YHL031C4.415.29E-6GOS1v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28
YBR284W_p4.298.92E-6YBR284W_pPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication
YBR182C-A_p4.221.21E-5YBR182C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YGL054C4.221.22E-5ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YML009C-A_d4.131.83E-5YML009C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBL071C-B_p3.865.67E-5YBL071C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YLR027C3.816.91E-5AAT2Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells

GO enrichment analysis for SGTC_1981
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1165.93E-19SGTC_2405hr heat shock (37°C) + Streptovitacin 300.0 μMMiscellaneous914670.0909091heat shock/prefoldin
0.0766.10E-9SGTC_2734clemastine 41.6 μMMiscellaneous269870.111111ERG2
0.0741.01E-8SGTC_14843473-2379 286.0 μMChemDiv (Drug-like library)6719170.137931
0.0691.15E-7SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.116883heat shock/prefoldin
0.0655.89E-7SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.108108copper-dependent oxidative stress
0.0631.49E-6SGTC_1850st056238 17.0 μMTimTec (Natural product derivative library)53931770.13253RPP1 & pyrimidine depletion
0.0631.53E-6SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.0879121
0.0621.74E-6SGTC_12490481-0015 10.2 μMChemDiv (Drug-like library)42275770.109756
0.0622.09E-6SGTC_1518st038567 90.8 μMTimTec (Pure natural product library)779660.116883
0.0612.47E-6SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.0972222cell wall signaling
0.0571.06E-5SGTC_30919116372 49.5 μMChembridge (Drug-like library)171697930.114943
0.0571.16E-5SGTC_21345324836 1.5 μMChembridge (Fragment library)6992720.0769231
0.0571.26E-5SGTC_1846st056244 14.8 μMTimTec (Natural product derivative library)53931820.114943
0.0552.20E-5SGTC_15852',4'-dihydroxychalcone 10.4 μMTimTec (Pure natural product library)53572180.115385
0.0543.78E-5SGTC_27895281112 71.4 μMChembridge (Drug-like library)7659480.109756

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1983st07181346.9 μM0.66666711838595TimTec (Natural product derivative library)426.247382.35827RPP1 & pyrimidine depletion
SGTC_1986st07181245 μM0.62857111838594TimTec (Natural product derivative library)426.247382.35827
SGTC_1988st07187253.4 μM0.58333316414116TimTec (Natural product derivative library)374.301660.19729
SGTC_1979st07180945.3 μM0.5342474976333TimTec (Natural product derivative library)398.194221.63127RPP1 & pyrimidine depletion
SGTC_1989st07186850.2 μM0.53424716414057TimTec (Natural product derivative library)398.194221.63127
SGTC_1980st07181145.7 μM0.524974850TimTec (Natural product derivative library)412.22081.9827RPP1 & pyrimidine depletion
SGTC_2415st07733357.7 μM0.442766255TimTec (Natural product derivative library)346.336161.23426
SGTC_481bay k-864450 μM0.3164562303ICCB bioactive library356.296512.67318
SGTC_1917st06650728.5 μM0.3103453156428TimTec (Natural product derivative library)399.3443063.33218Golgi
SGTC_20054024620188 μM0.264706236743Chembridge (Fragment library)179.215721.66513