st072290

ethyl 3-benzyl-4-hydroxy-2-oxo-1H-quinoline-6-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1987
Screen concentration 61.9 μM
Source TimTec (Natural product derivative library)
PubChem CID 54697379
SMILES CCOC(=O)C1=CC2=C(C=C1)NC(=O)C(=C2O)CC3=CC=CC=C3
Standardized SMILES CCOC(=O)c1ccc2nc(O)c(Cc3ccccc3)c(O)c2c1
Molecular weight 323.3426
ALogP 2.85
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.42
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 54697379
Download HIP data (tab-delimited text)  (excel)
Gene:EMW1(YNL313C)|FD-Score:3.87|P-value:5.43E-5|Clearance:0.01||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:ERG29(YMR134W_p)|FD-Score:3.86|P-value:5.60E-5|Clearance:0.15||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:RET3(YPL010W)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.43||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPT1(YKL145W)|FD-Score:4.92|P-value:4.23E-7|Clearance:1.05||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:TBF1(YPL128C)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.18||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:TFC6(YDR362C)|FD-Score:3.28|P-value:5.12E-4|Clearance:0.01||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:YDL196W(YDL196W_d)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:EMW1(YNL313C)|FD-Score:3.87|P-value:5.43E-5|Clearance:0.01||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:ERG29(YMR134W_p)|FD-Score:3.86|P-value:5.60E-5|Clearance:0.15||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:RET3(YPL010W)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.43||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPT1(YKL145W)|FD-Score:4.92|P-value:4.23E-7|Clearance:1.05||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:TBF1(YPL128C)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.18||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:TFC6(YDR362C)|FD-Score:3.28|P-value:5.12E-4|Clearance:0.01||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:YDL196W(YDL196W_d)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 54697379
Download HOP data (tab-delimited text)  (excel)
Gene:ARL1(YBR164C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:BEM2(YER155C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BEM3(YPL115C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:CBS2(YDR197W)|FD-Score:-4.55|P-value:2.66E-6||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CHS7(YHR142W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CMK2(YOL016C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:CPR3(YML078W)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:CWC21(YDR482C)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DNL4(YOR005C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:ELG1(YOR144C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EPT1(YHR123W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:ERD1(YDR414C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:GEA2(YEL022W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GRX6(YDL010W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GSC2(YGR032W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GUD1(YDL238C)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HSL7(YBR133C)|FD-Score:4.3|P-value:8.72E-6||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HST2(YPL015C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HYR1(YIR037W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:INP1(YMR204C)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC23(YOR044W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:MEI4(YER044C-A)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MMP1(YLL061W)|FD-Score:-4.36|P-value:6.45E-6||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MNN2(YBR015C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPS28(YDR337W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSE1(YOL033W)|FD-Score:6.21|P-value:2.71E-10||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:ODC2(YOR222W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:OPI11(YPR044C_d)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PAI3(YMR174C)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PIF1(YML061C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PKH1(YDR490C)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PPH22(YDL188C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:4.78|P-value:8.72E-7||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:SLI15(YBR156C)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SNQ2(YDR011W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPS19(YNL202W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SRL3(YKR091W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SSN8(YNL025C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STM1(YLR150W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TVP15(YDR100W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UGA3(YDL170W)|FD-Score:4.82|P-value:7.34E-7||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VAM10(YOR068C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:WSS1(YHR134W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBL062W(YBL062W_d)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL083C(YBL083C_d)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR027C(YBR027C_d)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL159W-A(YDL159W-A_p)|FD-Score:8.42|P-value:1.83E-17||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YGR001C(YGR001C_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR280C(YLR280C_d)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function Gene:YNL115C(YNL115C_p)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL216W(YPL216W_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPL257W(YPL257W_p)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPQ1(YOL092W_p)|FD-Score:4.89|P-value:4.96E-7||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR059C(YPR059C_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR123C(YPR123C_d)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ARL1(YBR164C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:BEM2(YER155C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BEM3(YPL115C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:CBS2(YDR197W)|FD-Score:-4.55|P-value:2.66E-6||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CHS7(YHR142W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CMK2(YOL016C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:CPR3(YML078W)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:CWC21(YDR482C)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DNL4(YOR005C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:ELG1(YOR144C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EPT1(YHR123W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:ERD1(YDR414C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:GEA2(YEL022W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GRX6(YDL010W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GSC2(YGR032W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GUD1(YDL238C)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HSL7(YBR133C)|FD-Score:4.3|P-value:8.72E-6||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HST2(YPL015C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HYR1(YIR037W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:INP1(YMR204C)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC23(YOR044W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:MEI4(YER044C-A)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MMP1(YLL061W)|FD-Score:-4.36|P-value:6.45E-6||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MNN2(YBR015C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPS28(YDR337W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSE1(YOL033W)|FD-Score:6.21|P-value:2.71E-10||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:ODC2(YOR222W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:OPI11(YPR044C_d)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PAI3(YMR174C)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PIF1(YML061C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PKH1(YDR490C)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PPH22(YDL188C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:4.78|P-value:8.72E-7||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:SLI15(YBR156C)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SNQ2(YDR011W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPS19(YNL202W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SRL3(YKR091W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SSN8(YNL025C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STM1(YLR150W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TVP15(YDR100W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UGA3(YDL170W)|FD-Score:4.82|P-value:7.34E-7||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VAM10(YOR068C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:WSS1(YHR134W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBL062W(YBL062W_d)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL083C(YBL083C_d)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR027C(YBR027C_d)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL159W-A(YDL159W-A_p)|FD-Score:8.42|P-value:1.83E-17||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YGR001C(YGR001C_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR280C(YLR280C_d)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function Gene:YNL115C(YNL115C_p)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL216W(YPL216W_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPL257W(YPL257W_p)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPQ1(YOL092W_p)|FD-Score:4.89|P-value:4.96E-7||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR059C(YPR059C_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR123C(YPR123C_d)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL145W4.924.23E-71.05RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YNL313C3.875.43E-50.01EMW1Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress
YMR134W_p3.865.60E-50.15ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YPL010W3.721.01E-40.43RET3Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YDR362C3.285.12E-40.01TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YPL128C3.275.31E-40.18TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YIL142W3.090.001010.08CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YER126C3.010.001300.01NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YLR078C3.000.001360.02BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
YER048W-A2.980.001440.01ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YMR197C2.970.001470.01VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YDR060W2.960.001526.52E-5MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
YNL149C2.960.001520.02PGA2Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
YJR072C2.940.001650.11NPA3Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
YJL069C2.830.002340.03UTP18Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL159W-A_p8.421.83E-17YDL159W-A_pPutative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
YOL033W6.212.71E-10MSE1Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated
YOL092W_p4.894.96E-7YPQ1_pPutative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter
YDL170W4.827.34E-7UGA3Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus
YDL136W4.788.72E-7RPL35BRibosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
YHR134W4.711.23E-6WSS1Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response
YIR037W4.671.50E-6HYR1Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor
YBR156C4.532.97E-6SLI15Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly
YDR482C4.434.72E-6CWC21Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p
YBR133C4.308.72E-6HSL7Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress
YDR011W4.261.02E-5SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YOR222W4.141.73E-5ODC2Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism
YNL202W4.131.81E-5SPS19Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
YDR337W4.013.03E-5MRPS28Mitochondrial ribosomal protein of the small subunit
YPL257W_p3.875.45E-5YPL257W_pPutative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene

GO enrichment analysis for SGTC_1987
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1056.44E-16SGTC_1984st072354 53.1 μMTimTec (Natural product derivative library)5762938NA
0.1033.03E-15SGTC_20422-0095 29.0 μMChemDiv (Drug-like library)6790840NAredox potentiating
0.1017.24E-15SGTC_162k030-0015 37.8 μMChemDiv (Drug-like library)1809920NAiron homeostasis
0.0881.61E-11SGTC_8590439-0102 54.6 μMChemDiv (Drug-like library)610143NAredox potentiating
0.0681.46E-7SGTC_1996st072960 66.8 μMTimTec (Natural product derivative library)1719405NA
0.0681.47E-7SGTC_2000st075254 36.4 μMTimTec (Natural product derivative library)17234175NA
0.0672.13E-7SGTC_1985st071382 65.5 μMTimTec (Natural product derivative library)6123237NA
0.0672.97E-7SGTC_30979117246 49.5 μMChembridge (Drug-like library)16423892NA
0.0649.01E-7SGTC_1097itavastatin 7.2 μMNIH Clinical Collection5282451NAazole & statin
0.0622.29E-6SGTC_14023909-7673 35.9 μMChemDiv (Drug-like library)5328014NA
0.0561.56E-5SGTC_2744captopril 92.0 μMMiscellaneous44093NA60S ribosome export
0.0552.25E-5SGTC_28107959871 45.5 μMChembridge (Drug-like library)2974535NAcopper-dependent oxidative stress
0.0552.59E-5SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)2832013NA60S ribosome export
0.0534.69E-5SGTC_1856st057054 55.7 μMTimTec (Natural product derivative library)1266018NA
0.0534.82E-5SGTC_23175986382 200.0 μMChembridge (Fragment library)877883NA

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature