st071872

methyl 2-amino-6-(hydroxymethyl)-4-(2-nitrophenyl)-8-oxo-4H-pyrano[3,2-b]pyran-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1988
Screen concentration 53.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 16414116
SMILES COC(=O)C1=C(OC2=C(C1C3=CC=CC=C3[N+](=O)[O-])OC(=CC2=O)CO)N
Standardized SMILES COC(=O)C1=C(N)OC2=C(OC(=CC2=O)CO)C1c3ccccc3[N+](=O)[O-]
Molecular weight 374.3017
ALogP 0.2
H-bond donor count 2
H-bond acceptor count 9
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.42
% growth inhibition (Hom. pool) 1.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16414116
Download HIP data (tab-delimited text)  (excel)
Gene:MEC1(YBR136W)|FD-Score:-3.88|P-value:5.25E-5|Clearance:0||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:YIP1(YGR172C)|FD-Score:3.85|P-value:6.00E-5|Clearance:0.77||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:YPR177C(YPR177C_d)|FD-Score:-3.23|P-value:6.15E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:MEC1(YBR136W)|FD-Score:-3.88|P-value:5.25E-5|Clearance:0||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:YIP1(YGR172C)|FD-Score:3.85|P-value:6.00E-5|Clearance:0.77||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:YPR177C(YPR177C_d)|FD-Score:-3.23|P-value:6.15E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16414116
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:AIM39(YOL053W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss Gene:APE3(YBR286W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:AVT1(YJR001W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI1(YNL271C)|FD-Score:-4|P-value:3.18E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BOI1(YBL085W)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:CLN1(YMR199W)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CTS1(YLR286C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DAT1(YML113W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:EAF3(YPR023C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:ERP3(YDL018C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FET4(YMR319C)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GIN4(YDR507C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GYP7(YDL234C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:IGO2(YHR132W-A)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:LEM3(YNL323W)|FD-Score:-4.68|P-value:1.40E-6||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MBF1(YOR298C-A)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MRH4(YGL064C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MSE1(YOL033W)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MSL1(YIR009W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:NPR1(YNL183C)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:PCL1(YNL289W)|FD-Score:5.09|P-value:1.75E-7||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX10(YDR265W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PGD1(YGL025C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:RGL1(YPL066W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPO41(YFL036W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTS1(YOR014W)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SIT4(YDL047W)|FD-Score:5.53|P-value:1.63E-8||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLG1(YOR008C)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:STP1(YDR463W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TOM70(YNL121C)|FD-Score:-4.01|P-value:2.99E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:UGA3(YDL170W)|FD-Score:6.49|P-value:4.33E-11||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS17(YOR132W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YBL055C(YBL055C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR141C(YBR141C_p)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR284W(YBR284W_p)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR056C(YDR056C_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YEH1(YLL012W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:YGR122C-A(YGR122C-A_d)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YHR097C(YHR097C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YML047W-A(YML047W-A_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL011C(YNL011C_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YOL050C(YOL050C_d)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR1(YGR281W)|FD-Score:-4.32|P-value:7.77E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:ZRT2(YLR130C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:ABZ2(YMR289W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:AIM39(YOL053W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss Gene:APE3(YBR286W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:AVT1(YJR001W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI1(YNL271C)|FD-Score:-4|P-value:3.18E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BOI1(YBL085W)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:CLN1(YMR199W)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CTS1(YLR286C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DAT1(YML113W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:EAF3(YPR023C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:ERP3(YDL018C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FET4(YMR319C)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GIN4(YDR507C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GYP7(YDL234C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:IGO2(YHR132W-A)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:LEM3(YNL323W)|FD-Score:-4.68|P-value:1.40E-6||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MBF1(YOR298C-A)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MRH4(YGL064C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MSE1(YOL033W)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MSL1(YIR009W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:NPR1(YNL183C)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:PCL1(YNL289W)|FD-Score:5.09|P-value:1.75E-7||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX10(YDR265W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PGD1(YGL025C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:RGL1(YPL066W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPO41(YFL036W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTS1(YOR014W)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SIT4(YDL047W)|FD-Score:5.53|P-value:1.63E-8||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLG1(YOR008C)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:STP1(YDR463W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TOM70(YNL121C)|FD-Score:-4.01|P-value:2.99E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:UGA3(YDL170W)|FD-Score:6.49|P-value:4.33E-11||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS17(YOR132W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YBL055C(YBL055C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR141C(YBR141C_p)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR284W(YBR284W_p)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR056C(YDR056C_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YEH1(YLL012W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:YGR122C-A(YGR122C-A_d)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YHR097C(YHR097C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YML047W-A(YML047W-A_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL011C(YNL011C_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YOL050C(YOL050C_d)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR1(YGR281W)|FD-Score:-4.32|P-value:7.77E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:ZRT2(YLR130C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR172C3.856.00E-50.77YIP1Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
YDR299W3.080.001050.03BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YKL060C3.040.001160.26FBA1Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress
YBL076C2.790.002670.31ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YOR250C2.470.006690.02CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YDR064W2.450.007140.01RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YGL073W2.440.007270.00HSF1Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated
YBR135W2.440.007300.01CKS1Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters
YDR088C2.430.007460.02SLU7RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
YJR065C2.420.007820.05ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YER172C2.370.008850.05BRR2RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
YJL019W2.320.010200.00MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YGL171W2.320.010300.00ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YML043C2.310.010300.02RRN11Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YLR359W2.290.010900.04ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL170W6.494.33E-11UGA3Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus
YDL047W5.531.63E-8SIT4Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization
YNL289W5.091.75E-7PCL1Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth
YJR001W4.241.12E-5AVT1Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YML113W4.231.16E-5DAT1DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability
YBR141C_p4.221.22E-5YBR141C_pPutative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
YBL055C4.161.62E-5YBL055C3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
YIR009W4.102.06E-5MSL1U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
YGR122C-A_d4.072.32E-5YGR122C-A_dDubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
YLR286C3.993.37E-5CTS1Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
YMR199W3.797.56E-5CLN1G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
YNL183C3.797.59E-5NPR1Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
YLR130C3.701.09E-4ZRT2Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor
YOR132W3.601.59E-4VPS17Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes
YDL018C3.492.39E-4ERP3Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport

GO enrichment analysis for SGTC_1988
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0741.41E-8SGTC_1996st072960 66.8 μMTimTec (Natural product derivative library)17194050.0786517
0.0604.22E-6SGTC_1650st011916 45.8 μMTimTec (Natural product derivative library)2425280.15
0.0604.33E-6SGTC_12700831-1170 17.3 μMChemDiv (Drug-like library)67647160.103448
0.0587.27E-6SGTC_2563tomatine 8.5 μMMicrosource (Natural product library)67087770.0542636
0.0571.21E-5SGTC_1998st075797 76.8 μMTimTec (Natural product derivative library)242083150.127907mitochondrial response to ROS
0.0543.86E-5SGTC_11323456-3614 2.5 μMChemDiv (Drug-like library)31255020.0957447tubulin folding & SWR complex
0.0501.39E-4SGTC_5221273-0010 11.6 μMChemDiv (Drug-like library)57050150.13095260S ribosome export
0.0491.81E-4SGTC_12400356-0016 42.1 μMChemDiv (Drug-like library)43056130.114943mitochondrial response to ROS
0.0491.90E-4SGTC_1878temozolomide 1.0 mMMiscellaneous53940.0987654
0.0482.23E-4SGTC_6033794-0023 45.9 μMChemDiv (Drug-like library)38022600.0813954
0.0464.26E-4SGTC_13291447-1697 50.6 μMChemDiv (Drug-like library)28405150.115789
0.0455.50E-4SGTC_1938st074717 56.4 μMTimTec (Natural product derivative library)172509790.108696
0.0455.77E-4SGTC_12490481-0015 10.2 μMChemDiv (Drug-like library)42275770.1125
0.0446.44E-4SGTC_9551216-0110 33.0 μMChemDiv (Drug-like library)36197190.13253sphingolipid biosynthesis & PDR1
0.0430.00104SGTC_28549024559 71.4 μMChembridge (Drug-like library)31635290.150538

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1979st07180945.3 μM0.6417914976333TimTec (Natural product derivative library)398.194221.63127RPP1 & pyrimidine depletion
SGTC_1989st07186850.2 μM0.64179116414057TimTec (Natural product derivative library)398.194221.63127
SGTC_1981st07182647 μM0.58333311838600TimTec (Natural product derivative library)425.355231.971210
SGTC_1980st07181145.7 μM0.5342474974850TimTec (Natural product derivative library)412.22081.9827RPP1 & pyrimidine depletion
SGTC_1983st07181346.9 μM0.52702711838595TimTec (Natural product derivative library)426.247382.35827RPP1 & pyrimidine depletion
SGTC_1986st07181245 μM0.49333311838594TimTec (Natural product derivative library)426.247382.35827
SGTC_2415st07733357.7 μM0.4520552766255TimTec (Natural product derivative library)346.336161.23426
SGTC_1080nisoldipine24.4 μM0.4246584499Miscellaneous388.414362.95817TSC3-RPN4
SGTC_481bay k-864450 μM0.3246752303ICCB bioactive library356.296512.67318
SGTC_1917st06650728.5 μM0.3023263156428TimTec (Natural product derivative library)399.3443063.33218Golgi