st073995

2-butyl-11b-methyl-1,2,3,5,6,11-hexahydroindolizino[8,7-b]indole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1993
Screen concentration 70.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 3098394
SMILES CCCCC1CC2(C3=C(CCN2C1)C4=CC=CC=C4N3)C
Standardized SMILES CCCCC1CN2CCc3c([nH]c4ccccc34)C2(C)C1
Molecular weight 282.4231
ALogP 4.95
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.9
% growth inhibition (Hom. pool) 5.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3098394
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:-3.17|P-value:7.67E-4|Clearance:0||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CDC27(YBL084C)|FD-Score:5.57|P-value:1.28E-8|Clearance:0.85||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ERG27(YLR100W)|FD-Score:3.5|P-value:2.32E-4|Clearance:0.03||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:GNA1(YFL017C)|FD-Score:-4.59|P-value:2.18E-6|Clearance:0||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:NEO1(YIL048W)|FD-Score:4.56|P-value:2.60E-6|Clearance:0.34||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NIC96(YFR002W)|FD-Score:4.21|P-value:1.25E-5|Clearance:0.58||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PRP24(YMR268C)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.02||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RIB5(YBR256C)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.04||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPL10(YLR075W)|FD-Score:6.17|P-value:3.36E-10|Clearance:0.85||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPN3(YER021W)|FD-Score:4.64|P-value:1.74E-6|Clearance:0.08||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RRP1(YDR087C)|FD-Score:3.42|P-value:3.19E-4|Clearance:0.39||SGD DESC:Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Gene:YGR114C(YGR114C_d)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YNL114C(YNL114C_d)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:ABF1(YKL112W)|FD-Score:-3.17|P-value:7.67E-4|Clearance:0||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CDC27(YBL084C)|FD-Score:5.57|P-value:1.28E-8|Clearance:0.85||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ERG27(YLR100W)|FD-Score:3.5|P-value:2.32E-4|Clearance:0.03||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:GNA1(YFL017C)|FD-Score:-4.59|P-value:2.18E-6|Clearance:0||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:NEO1(YIL048W)|FD-Score:4.56|P-value:2.60E-6|Clearance:0.34||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NIC96(YFR002W)|FD-Score:4.21|P-value:1.25E-5|Clearance:0.58||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PRP24(YMR268C)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.02||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RIB5(YBR256C)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.04||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPL10(YLR075W)|FD-Score:6.17|P-value:3.36E-10|Clearance:0.85||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPN3(YER021W)|FD-Score:4.64|P-value:1.74E-6|Clearance:0.08||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RRP1(YDR087C)|FD-Score:3.42|P-value:3.19E-4|Clearance:0.39||SGD DESC:Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Gene:YGR114C(YGR114C_d)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YNL114C(YNL114C_d)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3098394
Download HOP data (tab-delimited text)  (excel)
Gene:AGC1(YPR021C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:APL1(YJR005W)|FD-Score:6.04|P-value:7.62E-10||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:APT1(YML022W)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR5(YDR304C)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CTK3(YML112W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTS2(YDR371W_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:DPH1(YIL103W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ECM19(YLR390W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERV14(YGL054C)|FD-Score:4.94|P-value:3.99E-7||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GLR1(YPL091W)|FD-Score:4.47|P-value:3.92E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GTO3(YMR251W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:GZF3(YJL110C)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HEL2(YDR266C)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:INM2(YDR287W)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:INP1(YMR204C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC5(YFR038W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:MET22(YOL064C)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:NUP120(YKL057C)|FD-Score:8.24|P-value:8.57E-17||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OAF1(YAL051W)|FD-Score:5|P-value:2.80E-7||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OXP1(YKL215C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OYE3(YPL171C)|FD-Score:4.39|P-value:5.64E-6||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PAN5(YHR063C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PEP3(YLR148W)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET20(YPL159C)|FD-Score:-3.31|P-value:4.75E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX10(YDR265W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PRS2(YER099C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PUS5(YLR165C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAS2(YNL098C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RGP1(YDR137W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.83|P-value:6.89E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPA34(YJL148W)|FD-Score:-4.47|P-value:3.99E-6||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPL15B(YMR121C)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPL9B(YNL067W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS6B(YBR181C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:5.91|P-value:1.73E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SFP1(YLR403W)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SIW14(YNL032W)|FD-Score:3.81|P-value:7.04E-5||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SPT21(YMR179W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SWA2(YDR320C)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TAX4(YJL083W)|FD-Score:-4.46|P-value:4.11E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TDH1(YJL052W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TOM70(YNL121C)|FD-Score:6.27|P-value:1.85E-10||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPS2(YDR074W)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRR2(YHR106W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VPS1(YKR001C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:4.75|P-value:9.93E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.88|P-value:5.38E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBL044W(YBL044W_p)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBL062W(YBL062W_d)|FD-Score:5.44|P-value:2.68E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR221W-A(YBR221W-A_p)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YER010C(YER010C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YER152C(YER152C)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YFR006W(YFR006W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL218W(YGL218W_d)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR237C(YGR237C_p)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YNL155W(YNL155W_p)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YNL179C(YNL179C_d)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR093C(YOR093C_p)|FD-Score:-3.96|P-value:3.73E-5||SGD DESC:Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YPL185W(YPL185W_d)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR170C(YPR170C_d)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:AGC1(YPR021C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:APL1(YJR005W)|FD-Score:6.04|P-value:7.62E-10||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:APT1(YML022W)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR5(YDR304C)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CTK3(YML112W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTS2(YDR371W_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:DPH1(YIL103W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ECM19(YLR390W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERV14(YGL054C)|FD-Score:4.94|P-value:3.99E-7||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GLR1(YPL091W)|FD-Score:4.47|P-value:3.92E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GTO3(YMR251W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:GZF3(YJL110C)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HEL2(YDR266C)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:INM2(YDR287W)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:INP1(YMR204C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC5(YFR038W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:MET22(YOL064C)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:NUP120(YKL057C)|FD-Score:8.24|P-value:8.57E-17||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OAF1(YAL051W)|FD-Score:5|P-value:2.80E-7||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OXP1(YKL215C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OYE3(YPL171C)|FD-Score:4.39|P-value:5.64E-6||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PAN5(YHR063C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PEP3(YLR148W)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET20(YPL159C)|FD-Score:-3.31|P-value:4.75E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX10(YDR265W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PRS2(YER099C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PUS5(YLR165C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAS2(YNL098C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RGP1(YDR137W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.83|P-value:6.89E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPA34(YJL148W)|FD-Score:-4.47|P-value:3.99E-6||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPL15B(YMR121C)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPL9B(YNL067W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS6B(YBR181C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:5.91|P-value:1.73E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SFP1(YLR403W)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SIW14(YNL032W)|FD-Score:3.81|P-value:7.04E-5||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SPT21(YMR179W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SWA2(YDR320C)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TAX4(YJL083W)|FD-Score:-4.46|P-value:4.11E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TDH1(YJL052W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TOM70(YNL121C)|FD-Score:6.27|P-value:1.85E-10||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPS2(YDR074W)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRR2(YHR106W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VPS1(YKR001C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:4.75|P-value:9.93E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.88|P-value:5.38E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBL044W(YBL044W_p)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBL062W(YBL062W_d)|FD-Score:5.44|P-value:2.68E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR221W-A(YBR221W-A_p)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YER010C(YER010C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction Gene:YER152C(YER152C)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YFR006W(YFR006W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL218W(YGL218W_d)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR237C(YGR237C_p)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YNL155W(YNL155W_p)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YNL179C(YNL179C_d)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR093C(YOR093C_p)|FD-Score:-3.96|P-value:3.73E-5||SGD DESC:Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YPL185W(YPL185W_d)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR170C(YPR170C_d)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR075W6.173.36E-100.85RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YBL084C5.571.28E-80.85CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YNL114C_d4.721.16E-60.08YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YER021W4.641.74E-60.08RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YIL048W4.562.60E-60.34NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YFR002W4.211.25E-50.58NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YGR114C_d3.631.41E-40.13YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YLR100W3.502.32E-40.03ERG273-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
YBR256C3.472.56E-40.04RIB5Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
YMR268C3.432.98E-40.02PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YDR087C3.423.19E-40.39RRP1Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles
YPR016C3.030.001230.05TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YIL142W2.980.001440.10CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGR274C2.880.002010.03TAF1TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression
YLR033W2.850.002200.00RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL057C8.248.57E-17NUP120Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
YNL121C6.271.85E-10TOM70Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication
YJR005W6.047.62E-10APL1Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
YKL212W5.911.73E-9SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YBL062W_d5.442.68E-8YBL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR266C5.052.18E-7HEL2RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
YAL051W5.002.80E-7OAF1Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication
YER152C4.963.59E-7YER152CProtein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene
YGL054C4.943.99E-7ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YGL095C4.759.93E-7VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YDR287W4.503.43E-6INM2Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
YMR204C4.483.69E-6INP1Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane
YPL091W4.473.92E-6GLR1Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
YPL171C4.395.64E-6OYE3Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death
YGL218W_d4.376.26E-6YGL218W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance

GO enrichment analysis for SGTC_1993
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1633.06E-36SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.106383NEO1
0.1533.90E-32SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.0789474NEO1
0.1446.39E-29SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.0606061NEO1
0.1441.08E-28SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.106667NEO1
0.1381.74E-26SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0967742NEO1
0.1374.03E-26SGTC_1680diphenhydramine 100.0 μMNIH Clinical Collection89800.109375NEO1
0.1331.37E-24SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.108434NEO1
0.1322.07E-24SGTC_22577960413 200.0 μMChembridge (Fragment library)29746890.140625NEO1-PIK1
0.1291.84E-23SGTC_2697mebhydrolin 72.4 μMTimTec (Natural product derivative library)225300.202899NEO1
0.1293.11E-23SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.139241ERG2
0.1279.99E-23SGTC_9403232-1287 54.8 μMChemDiv (Drug-like library)15854930.0666667NEO1
0.1263.17E-22SGTC_270tamoxifen 5.5 μMMiscellaneous53760.109589NEO1
0.1231.75E-21SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.106667NEO1
0.1202.36E-20SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.142857endomembrane recycling
0.1202.94E-20SGTC_20365160441 105.0 μMChembridge (Fragment library)1345340.174603NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1636st00770768.9 μM0.298507403051TimTec (Natural product derivative library)290.359023.75911
SGTC_1954st07703268.9 μM0.279412698907TimTec (Natural product derivative library)290.359023.68611
SGTC_1953st07680012.1 μM0.275862297591TimTec (Natural product derivative library)219.281124.53410
SGTC_1524corynanthine62.1 μM0.27518528820TimTec (Pure natural product library)408.918962.99745
SGTC_20155119600184 μM0.2741942828786Chembridge (Fragment library)200.236482.07521
SGTC_6480375-047333.3 μM0.2459021713139ChemDiv (Drug-like library)230.348585.0111
SGTC_489paxilline115 μM0.244186105008ICCB bioactive library435.555223.934PDR1
SGTC_20515263410176 μM0.238095227584Chembridge (Fragment library)229.320762.15412
SGTC_407eburnamonine100 μM0.2368423195Miscellaneous294.390783.82402
SGTC_2696st07769174.32 μM0.234375757133TimTec (Natural product derivative library)200.279542.83611