st072814

4-hydroxy-5-nitro-2-[(Z)-2-(2-oxochromen-3-yl)ethenyl]-1H-pyrimidin-6-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1995
Screen concentration 61.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 5769371
SMILES C1=CC=C2C(=C1)C=C(C(=O)O2)C=CC3=NC(=C(C(=O)N3)[N+](=O)[O-])O
Standardized SMILES Oc1nc(C=CC2=Cc3ccccc3OC2=O)nc(O)c1[N+](=O)[O-]
Molecular weight 327.2485
ALogP 1.6
H-bond donor count 2
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.89
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5769371
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:3.23|P-value:6.13E-4|Clearance:0.15||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC21(YOR074C)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.08||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:PGA2(YNL149C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.27||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:PRE6(YOL038W)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PUP1(YOR157C)|FD-Score:4.66|P-value:1.58E-6|Clearance:1.07||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:ROK1(YGL171W)|FD-Score:-3.34|P-value:4.23E-4|Clearance:0||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPS13(YDR064W)|FD-Score:5.63|P-value:8.99E-9|Clearance:1.07||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:TIF5(YPR041W)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:YPL251W(YPL251W_d)|FD-Score:-3.35|P-value:4.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:CCT2(YIL142W)|FD-Score:3.23|P-value:6.13E-4|Clearance:0.15||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC21(YOR074C)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.08||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:PGA2(YNL149C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.27||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:PRE6(YOL038W)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PUP1(YOR157C)|FD-Score:4.66|P-value:1.58E-6|Clearance:1.07||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:ROK1(YGL171W)|FD-Score:-3.34|P-value:4.23E-4|Clearance:0||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPS13(YDR064W)|FD-Score:5.63|P-value:8.99E-9|Clearance:1.07||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:TIF5(YPR041W)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:YPL251W(YPL251W_d)|FD-Score:-3.35|P-value:4.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5769371
Download HOP data (tab-delimited text)  (excel)
Gene:AFR1(YDR085C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication Gene:ALG6(YOR002W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:AVT1(YJR001W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNA4(YBL098W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CUE1(YMR264W)|FD-Score:4.4|P-value:5.51E-6||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EXO5(YBR163W)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FMP21(YBR269C_p)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GDS1(YOR355W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPD2(YOL059W)|FD-Score:-4.04|P-value:2.63E-5||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HGH1(YGR187C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HIR1(YBL008W)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HIS7(YBR248C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HSP31(YDR533C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HXT10(YFL011W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:ITR2(YOL103W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:KNS1(YLL019C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LCB3(YJL134W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication Gene:MET17(YLR303W)|FD-Score:4.8|P-value:7.94E-7||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MNT4(YNR059W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRF1(YGL143C)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:PUS5(YLR165C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:QDR1(YIL120W)|FD-Score:-4.06|P-value:2.42E-5||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAD57(YDR004W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAM1(YDL090C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RIB4(YOL143C)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RPA34(YJL148W)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPL38(YLR325C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPL7A(YGL076C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:5.56|P-value:1.39E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAS3(YBL052C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SPO20(YMR017W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SPO21(YOL091W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SWC3(YAL011W)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TMA23(YMR269W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:VAM3(YOR106W)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:YBR284W(YBR284W_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDL129W(YDL129W_p)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR010C(YDR010C_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR096W(YJR096W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR075C(YKR075C_p)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR402W(YLR402W_d)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML009C-A(YML009C-A_d)|FD-Score:-4.42|P-value:4.90E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL190W(YNL190W_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YOP1(YPR028W)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR020W-A(YOR020W-A_p)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR015C(YPR015C_p)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR153W(YPR153W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Putative protein of unknown function Gene:YSC83(YHR017W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:YSC84(YHR016C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:AFR1(YDR085C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication Gene:ALG6(YOR002W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:AVT1(YJR001W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNA4(YBL098W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CUE1(YMR264W)|FD-Score:4.4|P-value:5.51E-6||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EXO5(YBR163W)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FMP21(YBR269C_p)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GDS1(YOR355W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPD2(YOL059W)|FD-Score:-4.04|P-value:2.63E-5||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HGH1(YGR187C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HIR1(YBL008W)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HIS7(YBR248C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HSP31(YDR533C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HXT10(YFL011W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:ITR2(YOL103W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:KNS1(YLL019C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LCB3(YJL134W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication Gene:MET17(YLR303W)|FD-Score:4.8|P-value:7.94E-7||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MNT4(YNR059W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRF1(YGL143C)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:PUS5(YLR165C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:QDR1(YIL120W)|FD-Score:-4.06|P-value:2.42E-5||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAD57(YDR004W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAM1(YDL090C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RIB4(YOL143C)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RPA34(YJL148W)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPL38(YLR325C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPL7A(YGL076C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:5.56|P-value:1.39E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAS3(YBL052C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SPO20(YMR017W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SPO21(YOL091W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SWC3(YAL011W)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TMA23(YMR269W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:VAM3(YOR106W)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:YBR284W(YBR284W_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDL129W(YDL129W_p)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR010C(YDR010C_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR096W(YJR096W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR075C(YKR075C_p)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR402W(YLR402W_d)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML009C-A(YML009C-A_d)|FD-Score:-4.42|P-value:4.90E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL190W(YNL190W_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YOP1(YPR028W)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR020W-A(YOR020W-A_p)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR015C(YPR015C_p)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR153W(YPR153W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Putative protein of unknown function Gene:YSC83(YHR017W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:YSC84(YHR016C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR064W5.638.99E-91.07RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YOR157C4.661.58E-61.07PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YNL149C3.591.66E-40.27PGA2Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
YOR074C3.324.56E-40.08CDC21Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
YIL142W3.236.13E-40.15CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YFL017C3.090.001020.14GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YHL015W2.950.001610.04RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YOL130W2.900.001843.14E-4ALR1Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
YKL196C2.900.001840.04YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YDR166C2.870.002060.02SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YLR275W2.850.002190.15SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YLR215C2.700.003440.09CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
YBL105C2.610.004551.66E-4PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YDR464W2.610.004550.02SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YLR078C2.590.004800.01BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL093C5.561.39E-8RSM25Mitochondrial ribosomal protein of the small subunit
YBR269C_p5.151.33E-7FMP21_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBL008W5.131.48E-7HIR1Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores
YLR303W4.807.94E-7MET17Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis
YPR153W4.641.74E-6YPR153WPutative protein of unknown function
YBL098W4.572.48E-6BNA4Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease
YPR028W4.483.74E-6YOP1Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress
YMR264W4.405.51E-6CUE1Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
YOR106W4.386.06E-6VAM3Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
YOR355W4.337.49E-6GDS1Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR269W4.251.09E-5TMA23Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
YAL011W4.082.25E-5SWC3Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
YLR165C3.983.41E-5PUS5Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability
YGR187C3.963.71E-5HGH1Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress
YFL011W3.924.39E-5HXT10Putative hexose transporter, expressed at low levels and expression is repressed by glucose

GO enrichment analysis for SGTC_1995
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0881.56E-11SGTC_315k915-0449 187.0 μMChemDiv (Drug-like library)209702150.0957447
0.0862.89E-11SGTC_1990st072050 59.8 μMTimTec (Natural product derivative library)172339480.0864198
0.0764.62E-9SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0526316ERG2
0.0758.02E-9SGTC_1833st055984 71.0 μMTimTec (Natural product derivative library)6762880.148649
0.0758.15E-9SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0806452ERG2
0.0663.80E-7SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0568182ERG2
0.0654.93E-7SGTC_28419004761 39.0 μMChembridge (Drug-like library)29910390.0740741amide catabolism
0.0656.05E-7SGTC_1791st048313 56.1 μMTimTec (Natural product derivative library)12049760.0674157
0.0631.41E-6SGTC_23227413319 38.2 μMChembridge (Fragment library)18404000.140845ERAD & cell cycle
0.0622.09E-6SGTC_2724maprotiline 69.6 μMMiscellaneous40110.0649351ERG2
0.0612.40E-6SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.0795455ERG2
0.0603.33E-6SGTC_495decylubiquinone 155.0 μMICCB bioactive library29710.0410959copper-dependent oxidative stress
0.0603.48E-6SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.0879121ERG2
0.0604.37E-6SGTC_1749st037932 54.2 μMTimTec (Natural product derivative library)5944970.0681818
0.0595.33E-6SGTC_12640783-0040 158.0 μMChemDiv (Drug-like library)28557980.0833333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1820st05376518.6 μM0.3246753690829TimTec (Natural product derivative library)428.459845.4106RPP1 & pyrimidine depletion
SGTC_1702st02703851 μM0.3086425521182TimTec (Natural product derivative library)392.387984.31718
SGTC_2981391-072910 μM0.2968753237558ChemDiv (Drug-like library)263.247563.3104calcium & mitochondrial duress
SGTC_1958st07651341.9 μM0.292308732122TimTec (Natural product derivative library)262.26283.31360S ribosome export
SGTC_22047233102200 μM0.2878792172480Chembridge (Fragment library)229.231341.6841360S ribosome export
SGTC_1703st02698348.3 μM0.28751104023TimTec (Natural product derivative library)414.410125.02915
SGTC_1655st01254655.1 μM0.285714720985TimTec (Natural product derivative library)237.253342.63113
SGTC_1771st0488824.15 μM0.2739734580403TimTec (Natural product derivative library)299.278123.1315RPP1 & pyrimidine depletion
SGTC_1695st02691434.5 μM0.2727273289191TimTec (Natural product derivative library)380.43855.26814
SGTC_1628st00425277.7 μM0.268657692968TimTec (Natural product derivative library)257.28452.4713