st073024

2-phenyl-3H-triazolo[4,5-d]pyrimidin-7-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1997
Screen concentration 93.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 686268
SMILES C1=CC=C(C=C1)N2NC3=NC=NC(=O)C3=N2
Standardized SMILES Oc1ncnc2nn(nc12)c3ccccc3
Molecular weight 213.1955
ALogP 0.59
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.24
% growth inhibition (Hom. pool) 4.05


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 686268
Download HIP data (tab-delimited text)  (excel)
Gene:CDC27(YBL084C)|FD-Score:-3.24|P-value:5.89E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC31(YOR257W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.03||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:GLE1(YDL207W)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.16||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:INO80(YGL150C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.42||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:MTR3(YGR158C)|FD-Score:4.6|P-value:2.12E-6|Clearance:0.55||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NEO1(YIL048W)|FD-Score:-3.16|P-value:7.85E-4|Clearance:0||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PRE6(YOL038W)|FD-Score:4.17|P-value:1.51E-5|Clearance:0.55||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RRB1(YMR131C)|FD-Score:4.53|P-value:3.02E-6|Clearance:0.55||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SEC31(YDL195W)|FD-Score:-3.73|P-value:9.74E-5|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:YPR177C(YPR177C_d)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:CDC27(YBL084C)|FD-Score:-3.24|P-value:5.89E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC31(YOR257W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.03||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:GLE1(YDL207W)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.16||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:INO80(YGL150C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.42||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:MTR3(YGR158C)|FD-Score:4.6|P-value:2.12E-6|Clearance:0.55||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NEO1(YIL048W)|FD-Score:-3.16|P-value:7.85E-4|Clearance:0||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PRE6(YOL038W)|FD-Score:4.17|P-value:1.51E-5|Clearance:0.55||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RRB1(YMR131C)|FD-Score:4.53|P-value:3.02E-6|Clearance:0.55||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SEC31(YDL195W)|FD-Score:-3.73|P-value:9.74E-5|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:YPR177C(YPR177C_d)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 686268
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:APL1(YJR005W)|FD-Score:4.32|P-value:7.91E-6||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ATG14(YBR128C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:COX15(YER141W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:CSR1(YLR380W)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:EMP47(YFL048C)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FAR11(YNL127W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FCY2(YER056C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FLD1(YLR404W)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FMP21(YBR269C_p)|FD-Score:7.49|P-value:3.52E-14||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLT1(YDL171C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:HIR1(YBL008W)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:ISC1(YER019W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:LEU3(YLR451W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MFA2(YNL145W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MRPS28(YDR337W)|FD-Score:4.33|P-value:7.60E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MVB12(YGR206W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NNF2(YGR089W)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUP100(YKL068W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Gene:NUP120(YKL057C)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUP170(YBL079W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PEX22(YAL055W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PFK26(YIL107C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:RXT2(YBR095C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SNA4(YDL123W)|FD-Score:-3.93|P-value:4.31E-5||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SNT2(YGL131C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SOK1(YDR006C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPT3(YDR392W)|FD-Score:4.79|P-value:8.28E-7||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TLG2(YOL018C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOS6(YNL300W_p)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRS65(YGR166W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:VPS62(YGR141W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:YEL028W(YEL028W_d)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YHL017W(YHL017W_p)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YJL160C(YJL160C_p)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YML002W(YML002W_p)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YOL163W(YOL163W_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YSC84(YHR016C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ADO1(YJR105W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:APL1(YJR005W)|FD-Score:4.32|P-value:7.91E-6||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ATG14(YBR128C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:COX15(YER141W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:CSR1(YLR380W)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:EMP47(YFL048C)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FAR11(YNL127W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FCY2(YER056C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FLD1(YLR404W)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FMP21(YBR269C_p)|FD-Score:7.49|P-value:3.52E-14||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLT1(YDL171C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:HIR1(YBL008W)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:ISC1(YER019W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:LEU3(YLR451W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MFA2(YNL145W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MRPS28(YDR337W)|FD-Score:4.33|P-value:7.60E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MVB12(YGR206W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NNF2(YGR089W)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUP100(YKL068W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Gene:NUP120(YKL057C)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUP170(YBL079W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PEX22(YAL055W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PFK26(YIL107C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:RXT2(YBR095C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SNA4(YDL123W)|FD-Score:-3.93|P-value:4.31E-5||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SNT2(YGL131C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SOK1(YDR006C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPT3(YDR392W)|FD-Score:4.79|P-value:8.28E-7||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TLG2(YOL018C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOS6(YNL300W_p)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRS65(YGR166W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:VPS62(YGR141W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:YEL028W(YEL028W_d)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YHL017W(YHL017W_p)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YJL160C(YJL160C_p)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YML002W(YML002W_p)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YOL163W(YOL163W_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YSC84(YHR016C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR158C4.602.12E-60.55MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YMR131C4.533.02E-60.55RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YOL038W4.171.51E-50.55PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YOR257W3.621.47E-40.03CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YGL150C3.591.67E-40.42INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YDL207W3.167.77E-40.16GLE1Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
YML127W3.000.001340.16RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YDR299W2.840.002230.01BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YGL008C2.830.002310.06PMA1Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
YER127W2.770.002810.06LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YDR398W2.710.003400.00UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YIL051C2.710.003410.04MMF1Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes
YPL251W_d2.670.003840.00YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YJL011C2.660.003890.03RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YLL035W2.630.004270.03GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR269C_p7.493.52E-14FMP21_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YLR404W5.052.25E-7FLD1Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
YDR392W4.798.28E-7SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YHL017W_p4.483.78E-6YHL017W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
YDR337W4.337.60E-6MRPS28Mitochondrial ribosomal protein of the small subunit
YJR005W4.327.91E-6APL1Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
YNL145W4.131.83E-5MFA2Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
YNL127W4.052.56E-5FAR11Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2
YBR095C3.914.65E-5RXT2Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth
YFL048C3.895.02E-5EMP47Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport
YGR089W3.739.76E-5NNF2Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation
YDR006C3.651.30E-4SOK1Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13
YBL079W3.651.32E-4NUP170Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
YML002W_p3.601.56E-4YML002W_pPutative protein of unknown function; expression induced by heat and by calcium shortage
YIL107C3.492.46E-4PFK266-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A

GO enrichment analysis for SGTC_1997
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1285.04E-23SGTC_29329016711 44.5 μMChembridge (Drug-like library)71173280.0895522
0.0898.05E-12SGTC_1309pyrazolanthrone 13.4 μMChemDiv (Drug-like library)85150.18
0.0856.36E-11SGTC_1984st072354 53.1 μMTimTec (Natural product derivative library)57629380.0864198
0.0732.35E-8SGTC_30849116877 49.5 μMChembridge (Drug-like library)272413250.112676
0.0604.47E-6SGTC_12941000-1494 117.0 μMChemDiv (Drug-like library)7127970.131148
0.0595.04E-6SGTC_22947971431 200.0 μMChembridge (Fragment library)57415720.0491803
0.0595.23E-6SGTC_32459134821 49.5 μMChembridge (Drug-like library)170149010.177419
0.0595.63E-6SGTC_1746st038117 42.0 μMTimTec (Natural product derivative library)28982180.0365854
0.0571.33E-5SGTC_8160325-0417 81.3 μMChemDiv (Drug-like library)681010.0810811
0.0561.52E-5SGTC_29769009005 19.5 μMChembridge (Drug-like library)29931480.0909091
0.0552.54E-5SGTC_28589025781 9.0 μMChembridge (Drug-like library)48949800.133333sphingolipid biosynthesis & PDR1
0.0542.83E-5SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.0447761PDR1
0.0543.07E-5SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.112903
0.0543.10E-5SGTC_6281636-0253 16.0 μMChemDiv (Drug-like library)44561430.125sphingolipid biosynthesis & PDR1
0.0535.26E-5SGTC_12870971-0001 34.6 μMChemDiv (Drug-like library)46658690.101695sphingolipid biosynthesis & PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1943st07633165.2 μM0.31578924208415TimTec (Natural product derivative library)306.770642.72813
SGTC_1942st07633065.2 μM0.28070224208414TimTec (Natural product derivative library)306.770642.72813
SGTC_2707st07855666.6 μM0.27586217250899TimTec (Natural product derivative library)300.378742.89813
SGTC_1947st07633261.6 μM0.27419424208416TimTec (Natural product derivative library)324.7611032.93314
SGTC_2710st07856253.72 μM0.27272717250908TimTec (Natural product derivative library)357.430062.40724
SGTC_1948st07632966.6 μM0.26666724208413TimTec (Natural product derivative library)300.378743.03613
SGTC_3384055-0136114 μM0.2542372900563ChemDiv (Drug-like library)286.30581.93515
SGTC_2708st07855956 μM0.2517250902TimTec (Natural product derivative library)357.430062.43724
SGTC_2692st07742647.7 μM0.24285725238571TimTec (Natural product derivative library)419.499444.12124
SGTC_1944st07637156.9 μM0.23529424208455TimTec (Natural product derivative library)351.3312461.52726