st075797

2-methylpropyl N-[2-(1H-indol-3-yl)ethyl]carbamate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1998
Screen concentration 76.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 24208315
SMILES CC(C)COC(=O)NCCC1=CNC2=CC=CC=C21
Standardized SMILES CC(C)COC(=O)NCCc1c[nH]c2ccccc12
Molecular weight 260.3315
ALogP 3.41
H-bond donor count 2
H-bond acceptor count 2
Response signature mitochondrial response to ROS

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.53
% growth inhibition (Hom. pool) 8.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24208315
Download HIP data (tab-delimited text)  (excel)
Gene:CDC23(YHR166C)|FD-Score:-3.18|P-value:7.32E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC3(YLR314C)|FD-Score:-3.81|P-value:6.87E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CWC25(YNL245C)|FD-Score:3.12|P-value:9.06E-4|Clearance:0.07||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:ILS1(YBL076C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.13||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MCM5(YLR274W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.02||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:OKP1(YGR179C)|FD-Score:-3.18|P-value:7.47E-4|Clearance:0||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:PRP45(YAL032C)|FD-Score:3.88|P-value:5.15E-5|Clearance:0.35||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RAD53(YPL153C)|FD-Score:-5.18|P-value:1.08E-7|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RER2(YBR002C)|FD-Score:4.1|P-value:2.04E-5|Clearance:0.35||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RSC6(YCR052W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:TFC3(YAL001C)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRE2(YOR256C)|FD-Score:-4.07|P-value:2.36E-5|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YKT6(YKL196C)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YPL238C(YPL238C_d)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:CDC23(YHR166C)|FD-Score:-3.18|P-value:7.32E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC3(YLR314C)|FD-Score:-3.81|P-value:6.87E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CWC25(YNL245C)|FD-Score:3.12|P-value:9.06E-4|Clearance:0.07||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:ILS1(YBL076C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.13||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MCM5(YLR274W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.02||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:OKP1(YGR179C)|FD-Score:-3.18|P-value:7.47E-4|Clearance:0||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:PRP45(YAL032C)|FD-Score:3.88|P-value:5.15E-5|Clearance:0.35||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RAD53(YPL153C)|FD-Score:-5.18|P-value:1.08E-7|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RER2(YBR002C)|FD-Score:4.1|P-value:2.04E-5|Clearance:0.35||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RSC6(YCR052W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:TFC3(YAL001C)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRE2(YOR256C)|FD-Score:-4.07|P-value:2.36E-5|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YKT6(YKL196C)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YPL238C(YPL238C_d)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24208315
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:AIM36(YMR157C)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ALY1(YKR021W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:APS2(YJR058C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:ARO1(YDR127W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG15(YCR068W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BTS1(YPL069C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CRN1(YLR429W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CUE1(YMR264W)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CUR1(YPR158W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DMA2(YNL116W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DOT6(YER088C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ECM33(YBR078W)|FD-Score:5.66|P-value:7.60E-9||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FCY2(YER056C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FMP10(YER182W_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GDB1(YPR184W)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEP4(YHR100C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:ILM1(YJR118C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:LSM6(YDR378C)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MAP1(YLR244C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MEF2(YJL102W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MET10(YFR030W)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MKT1(YNL085W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:4.47|P-value:3.90E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRT4(YKL009W)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:NAT4(YMR069W)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:NCL1(YBL024W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NSI1(YDR026C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication Gene:PAU7(YAR020C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PEX27(YOR193W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PKR1(YMR123W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PRM6(YML047C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:RAV1(YJR033C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCK2(YLR248W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RDL1(YOR285W)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIP1(YEL024W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RPL20B(YOR312C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:9.1|P-value:4.44E-20||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RRD2(YPL152W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTS1(YOR014W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SCJ1(YMR214W)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SDC25(YLL016W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SPO75(YLL005C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SWM2(YNR004W_p)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TAT2(YOL020W)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TMA108(YIL137C)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TRK1(YJL129C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM44(YPL030W)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:TRP1(YDR007W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.14|P-value:4.02E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VID24(YBR105C)|FD-Score:3.09|P-value:0.00100||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS41(YDR080W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YBL071C-B(YBL071C-B_p)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR219C(YBR219C_p)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDR149C(YDR149C_d)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YIR018C-A(YIR018C-A_p)|FD-Score:5.55|P-value:1.40E-8||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YMR262W(YMR262W_p)|FD-Score:-4.61|P-value:2.06E-6||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNL095C(YNL095C_p)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YNR048W(YNR048W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YPR117W(YPR117W_p)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative protein of unknown function Gene:ABZ2(YMR289W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:AIM36(YMR157C)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ALY1(YKR021W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:APS2(YJR058C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:ARO1(YDR127W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG15(YCR068W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BTS1(YPL069C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CRN1(YLR429W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CUE1(YMR264W)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CUR1(YPR158W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DMA2(YNL116W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DOT6(YER088C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ECM33(YBR078W)|FD-Score:5.66|P-value:7.60E-9||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FCY2(YER056C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FMP10(YER182W_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GDB1(YPR184W)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEP4(YHR100C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:ILM1(YJR118C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:LSM6(YDR378C)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MAP1(YLR244C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MEF2(YJL102W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MET10(YFR030W)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MKT1(YNL085W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:4.47|P-value:3.90E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRT4(YKL009W)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:NAT4(YMR069W)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:NCL1(YBL024W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NSI1(YDR026C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication Gene:PAU7(YAR020C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PEX27(YOR193W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PKR1(YMR123W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PRM6(YML047C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:RAV1(YJR033C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCK2(YLR248W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RDL1(YOR285W)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIP1(YEL024W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RPL20B(YOR312C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:9.1|P-value:4.44E-20||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RRD2(YPL152W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTS1(YOR014W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SCJ1(YMR214W)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SDC25(YLL016W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SPO75(YLL005C)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SWM2(YNR004W_p)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TAT2(YOL020W)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TMA108(YIL137C)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TRK1(YJL129C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM44(YPL030W)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:TRP1(YDR007W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.14|P-value:4.02E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VID24(YBR105C)|FD-Score:3.09|P-value:0.00100||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS41(YDR080W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YBL071C-B(YBL071C-B_p)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR219C(YBR219C_p)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDR149C(YDR149C_d)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YIR018C-A(YIR018C-A_p)|FD-Score:5.55|P-value:1.40E-8||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YMR262W(YMR262W_p)|FD-Score:-4.61|P-value:2.06E-6||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNL095C(YNL095C_p)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YNR048W(YNR048W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YPR117W(YPR117W_p)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C4.102.04E-50.35RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YAL032C3.885.15E-50.35PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YCR052W3.532.07E-40.11RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YLR274W3.423.16E-40.02MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YPL238C_d3.393.44E-40.14YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YBL076C3.255.68E-40.14ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YNL245C3.129.06E-40.07CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YLR430W3.050.001160.17SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YGR128C2.880.002000.06UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YMR061W2.810.002450.04RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YPL131W2.780.002750.02RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YPL235W2.760.002930.07RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YMR208W2.680.003650.02ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YDR141C2.670.003830.07DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YJL010C2.600.004650.03NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFL036W9.104.44E-20RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YKL211C6.144.02E-10TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YBR078W5.667.60E-9ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YIR018C-A_p5.551.40E-8YIR018C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YFR030W5.071.96E-7MET10Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide
YBR219C_p4.741.08E-6YBR219C_pPutative protein of unknown function; YBR219C is not an essential gene
YPR117W_p4.641.72E-6YPR117W_pPutative protein of unknown function
YMR123W4.533.00E-6PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YLR439W4.473.90E-6MRPL4Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
YBL071C-B_p4.405.34E-6YBL071C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YOR285W4.289.54E-6RDL1Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress
YOR193W4.121.88E-5PEX27Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p
YDR149C_d4.082.25E-5YDR149C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth
YLR061W4.032.80E-5RPL22ARibosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
YDR354W3.993.29E-5TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis

GO enrichment analysis for SGTC_1998
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1991.23E-53SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0315789
0.1964.04E-52SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0857143
0.1892.33E-48SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0862069
0.1871.19E-47SGTC_18825615643 20.0 μMMiscellaneous22530750.0833333TRP & mitochondrial translation
0.1858.75E-47SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0681818
0.1843.35E-46SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0952381TRP & mitochondrial translation
0.1817.56E-45SGTC_18815652484 16.0 μMMiscellaneous22551290.097561TRP & mitochondrial translation
0.1778.90E-43SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.123288
0.1761.87E-42SGTC_12400356-0016 42.1 μMChemDiv (Drug-like library)43056130.138889mitochondrial response to ROS
0.1741.53E-41SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.0740741
0.1741.84E-41SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.151899
0.1742.62E-41SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.118421
0.1734.55E-41SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.0821918
0.1734.65E-41SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.106667
0.1731.03E-40SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0533333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1778st04681958.9 μM0.589286671715TimTec (Natural product derivative library)339.345283.69324
SGTC_1790st04571143.5 μM0.4375585119TimTec (Natural product derivative library)459.3848795.01539
SGTC_1677st01920453.9 μM0.4354843945625TimTec (Natural product derivative library)370.829461.06334
SGTC_1931st07443857.1 μM0.4126982787313TimTec (Natural product derivative library)350.410983.91323
SGTC_452diindolylmethane8 μM0.4090913071ICCB bioactive library246.306464.39120RNA processing & uracil transport
SGTC_2935903801629.96 μM0.46463195Chembridge (Drug-like library)296.3387833.92722
SGTC_2017513292760.65 μM0.3921578367Chembridge (Fragment library)188.268842.45121
SGTC_2588indole-3-carbinol100 μM0.3913043712Microsource (Natural product library)147.173861.51821
SGTC_2932901671144.54 μM0.3833337117328Chembridge (Drug-like library)308.331242.78423
SGTC_1694st02401144.9 μM0.3823532787533TimTec (Natural product derivative library)445.306444.01124ERG2