0481-0013

2-chloro-3-(chloromethyl)naphthalene-1,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_20
Screen concentration 4.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 182045
SMILES C1=CC=C2C(=C1)C(=O)C(=C(C2=O)Cl)CCl
Standardized SMILES ClCC1=C(Cl)C(=O)c2ccccc2C1=O
Molecular weight 241.0701
ALogP 2.69
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 56.14
% growth inhibition (Hom. pool) 11.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 182045
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC20(YGL116W)|FD-Score:4.04|P-value:2.70E-5|Clearance:0.16||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:DED1(YOR204W)|FD-Score:-4.78|P-value:8.67E-7|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ERG26(YGL001C)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.12||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:ERG7(YHR072W)|FD-Score:-3.8|P-value:7.23E-5|Clearance:0||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GLC7(YER133W)|FD-Score:-3.61|P-value:1.53E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.19||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:MET30(YIL046W)|FD-Score:7.13|P-value:4.87E-13|Clearance:1.21||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MEX67(YPL169C)|FD-Score:3.75|P-value:9.00E-5|Clearance:0.05||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NOP15(YNL110C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PRP24(YMR268C)|FD-Score:3.21|P-value:6.62E-4|Clearance:0.03||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPB3(YIL021W)|FD-Score:5.67|P-value:7.04E-9|Clearance:1.21||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL25(YOL127W)|FD-Score:3.79|P-value:7.66E-5|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL5(YPL131W)|FD-Score:3.88|P-value:5.20E-5|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPN3(YER021W)|FD-Score:3.17|P-value:7.68E-4|Clearance:0.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:SEC12(YNR026C)|FD-Score:6.45|P-value:5.69E-11|Clearance:1.21||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SWC4(YGR002C)|FD-Score:-3.36|P-value:3.90E-4|Clearance:0||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TFC1(YBR123C)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.11||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TOA2(YKL058W)|FD-Score:-3.24|P-value:5.94E-4|Clearance:0||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:ULP2(YIL031W)|FD-Score:3.11|P-value:9.32E-4|Clearance:0.1||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:YDR327W(YDR327W_d)|FD-Score:3.18|P-value:7.38E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Gene:YJL195C(YJL195C_d)|FD-Score:-3.27|P-value:5.42E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJL202C(YJL202C_d)|FD-Score:4.47|P-value:3.97E-6|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex Gene:YOR282W(YOR282W_d)|FD-Score:3.18|P-value:7.37E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:CDC19(YAL038W)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC20(YGL116W)|FD-Score:4.04|P-value:2.70E-5|Clearance:0.16||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:DED1(YOR204W)|FD-Score:-4.78|P-value:8.67E-7|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ERG26(YGL001C)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.12||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:ERG7(YHR072W)|FD-Score:-3.8|P-value:7.23E-5|Clearance:0||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GLC7(YER133W)|FD-Score:-3.61|P-value:1.53E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.19||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:MET30(YIL046W)|FD-Score:7.13|P-value:4.87E-13|Clearance:1.21||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MEX67(YPL169C)|FD-Score:3.75|P-value:9.00E-5|Clearance:0.05||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NOP15(YNL110C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PRP24(YMR268C)|FD-Score:3.21|P-value:6.62E-4|Clearance:0.03||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RPB3(YIL021W)|FD-Score:5.67|P-value:7.04E-9|Clearance:1.21||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL25(YOL127W)|FD-Score:3.79|P-value:7.66E-5|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL5(YPL131W)|FD-Score:3.88|P-value:5.20E-5|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPN3(YER021W)|FD-Score:3.17|P-value:7.68E-4|Clearance:0.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:SEC12(YNR026C)|FD-Score:6.45|P-value:5.69E-11|Clearance:1.21||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SWC4(YGR002C)|FD-Score:-3.36|P-value:3.90E-4|Clearance:0||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TFC1(YBR123C)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.11||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TOA2(YKL058W)|FD-Score:-3.24|P-value:5.94E-4|Clearance:0||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:ULP2(YIL031W)|FD-Score:3.11|P-value:9.32E-4|Clearance:0.1||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:YDR327W(YDR327W_d)|FD-Score:3.18|P-value:7.38E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Gene:YJL195C(YJL195C_d)|FD-Score:-3.27|P-value:5.42E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJL202C(YJL202C_d)|FD-Score:4.47|P-value:3.97E-6|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex Gene:YOR282W(YOR282W_d)|FD-Score:3.18|P-value:7.37E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 182045
Download HOP data (tab-delimited text)  (excel)
Gene:AIM20(YIL158W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:BDF1(YLR399C)|FD-Score:3.27|P-value:5.28E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BEM4(YPL161C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BIO2(YGR286C)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:CMR1(YDL156W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:CMR3(YPR013C_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COS10(YNR075W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CTK3(YML112W)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DRS2(YAL026C)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM4(YKR076W)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELP3(YPL086C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:FAR7(YFR008W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:GAD1(YMR250W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCY1(YOR120W)|FD-Score:5.3|P-value:5.78E-8||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GND1(YHR183W)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HIM1(YDR317W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HTA1(YDR225W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:HUG1(YML058W-A)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IDP3(YNL009W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:INO2(YDR123C)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:IRC15(YPL017C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:LAP3(YNL239W)|FD-Score:5.37|P-value:4.01E-8||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LSM1(YJL124C)|FD-Score:5.95|P-value:1.37E-9||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MDG1(YNL173C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MRPL44(YMR225C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSH3(YCR092C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NBA1(YOL070C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:OGG1(YML060W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PEP12(YOR036W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PMP3(YDR276C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:POA1(YBR022W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PRC1(YMR297W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRM5(YIL117C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PST2(YDR032C)|FD-Score:51.1|P-value:0||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RBK1(YCR036W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative ribokinase Gene:RPL20A(YMR242C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:SIS2(YKR072C)|FD-Score:5.93|P-value:1.54E-9||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLI1(YGR212W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SOD1(YJR104C)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPS4(YOR313C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SRO7(YPR032W)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSN3(YPL042C)|FD-Score:3.13|P-value:8.59E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STV1(YMR054W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TDH3(YGR192C)|FD-Score:-4.27|P-value:9.62E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:UBR1(YGR184C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VAM6(YDL077C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VPS24(YKL041W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS36(YLR417W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS8(YAL002W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YAL004W(YAL004W_d)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAL018C(YAL018C_p)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Putative protein of unknown function Gene:YBT1(YLL048C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCL001W-A(YCL001W-A_p)|FD-Score:5.39|P-value:3.56E-8||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YER134C(YER134C)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YER135C(YER135C_d)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YGR290W(YGR290W_d)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YGR291C(YGR291C_d)|FD-Score:6.75|P-value:7.18E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL141W(YIL141W_d)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR061W(YJR061W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL034W(YNL034W_p)|FD-Score:6.07|P-value:6.39E-10||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YNL095C(YNL095C_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YOR114W(YOR114W_p)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL039W(YPL039W_p)|FD-Score:5.48|P-value:2.16E-8||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL108W(YPL108W_p)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPL162C(YPL162C_p)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:ZRC1(YMR243C)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:AIM20(YIL158W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:BDF1(YLR399C)|FD-Score:3.27|P-value:5.28E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BEM4(YPL161C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BIO2(YGR286C)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:CMR1(YDL156W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:CMR3(YPR013C_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COS10(YNR075W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CTK3(YML112W)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DRS2(YAL026C)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM4(YKR076W)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELP3(YPL086C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:FAR7(YFR008W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:GAD1(YMR250W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCY1(YOR120W)|FD-Score:5.3|P-value:5.78E-8||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GND1(YHR183W)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HIM1(YDR317W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HTA1(YDR225W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:HUG1(YML058W-A)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IDP3(YNL009W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:INO2(YDR123C)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:IRC15(YPL017C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:LAP3(YNL239W)|FD-Score:5.37|P-value:4.01E-8||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LSM1(YJL124C)|FD-Score:5.95|P-value:1.37E-9||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MDG1(YNL173C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MRPL44(YMR225C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSH3(YCR092C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NBA1(YOL070C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:OGG1(YML060W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PEP12(YOR036W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PMP3(YDR276C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:POA1(YBR022W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PRC1(YMR297W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRM5(YIL117C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PST2(YDR032C)|FD-Score:51.1|P-value:0||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:RBK1(YCR036W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative ribokinase Gene:RPL20A(YMR242C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:SIS2(YKR072C)|FD-Score:5.93|P-value:1.54E-9||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLI1(YGR212W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SOD1(YJR104C)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPS4(YOR313C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SRO7(YPR032W)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSN3(YPL042C)|FD-Score:3.13|P-value:8.59E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STV1(YMR054W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TDH3(YGR192C)|FD-Score:-4.27|P-value:9.62E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:UBR1(YGR184C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VAM6(YDL077C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VPS24(YKL041W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS36(YLR417W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS8(YAL002W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YAL004W(YAL004W_d)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAL018C(YAL018C_p)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Putative protein of unknown function Gene:YBT1(YLL048C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCL001W-A(YCL001W-A_p)|FD-Score:5.39|P-value:3.56E-8||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YER134C(YER134C)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YER135C(YER135C_d)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YGR290W(YGR290W_d)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YGR291C(YGR291C_d)|FD-Score:6.75|P-value:7.18E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL141W(YIL141W_d)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR061W(YJR061W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL034W(YNL034W_p)|FD-Score:6.07|P-value:6.39E-10||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YNL095C(YNL095C_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YOR114W(YOR114W_p)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL039W(YPL039W_p)|FD-Score:5.48|P-value:2.16E-8||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL108W(YPL108W_p)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPL162C(YPL162C_p)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:ZRC1(YMR243C)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL046W7.134.87E-131.21MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YNR026C6.455.69E-111.21SEC12Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
YIL021W5.677.04E-91.21RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YJL202C_d4.473.97E-60.43YJL202C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
YGL116W4.042.70E-50.16CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YPL131W3.885.20E-50.10RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YOL127W3.797.66E-50.04RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YPL169C3.759.00E-50.05MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YBR123C3.691.10E-40.11TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YNL110C3.591.66E-40.07NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YJR013W3.522.13E-40.19GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YGL001C3.334.31E-40.12ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YMR268C3.216.62E-40.03PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YOR282W_d3.187.37E-44.10E-4YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YDR327W_d3.187.38E-40.01YDR327W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR032C51.100PST2Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
YGR291C_d6.757.18E-12YGR291C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YNL034W_p6.076.39E-10YNL034W_pPutative protein of unknown function; YNL034W is not an essential gene
YJL124C5.951.37E-9LSM1Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
YKR072C5.931.54E-9SIS2Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
YPL039W_p5.482.16E-8YPL039W_pPutative protein of unknown function; YPL039W is not an essential gene
YCL001W-A_p5.393.56E-8YCL001W-A_pPutative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III
YNL239W5.374.01E-8LAP3Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
YOR120W5.305.78E-8GCY1Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
YKR076W5.161.25E-7ECM4Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YMR225C4.721.20E-6MRPL44Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
YPR032W4.681.43E-6SRO7Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
YGR286C4.611.97E-6BIO2Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
YFR008W4.542.83E-6FAR7Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
YIL141W_d4.356.95E-6YIL141W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_20
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1977.14E-53SGTC_2521angolensin 13.4 μMMicrosource (Natural product library)8214280.0740741
0.1837.67E-46SGTC_8570438-0306 1.4 μMChemDiv (Drug-like library)46861360.105263copper-dependent oxidative stress
0.1291.91E-23SGTC_11483909-7663 27.7 μMChemDiv (Drug-like library)40845260.0909091
0.1285.68E-23SGTC_1060092-0003 11.6 μMChemDiv (Drug-like library)10721480.255814copper-dependent oxidative stress
0.1271.47E-22SGTC_2608thymoquinone 42.7 μMMicrosource (Natural product library)102810.102564
0.1151.14E-18SGTC_10214476-0760 85.3 μMChemDiv (Drug-like library)7467680.142857
0.1095.13E-17SGTC_1562morin hydrate 59.1 μMTimTec (Pure natural product library)185421360.142857
0.1071.45E-16SGTC_24345670830 169.9 μMMiscellaneous15498300.150943
0.1041.00E-15SGTC_30579095946 49.5 μMChembridge (Drug-like library)245400430.101695Golgi
0.1024.24E-15SGTC_24315490079 200.0 μMMiscellaneous57223450.0925926
0.1001.55E-14SGTC_6221348-1566 26.3 μMChemDiv (Drug-like library)73404070.125
0.0896.63E-12SGTC_1865st057639 52.2 μMTimTec (Natural product derivative library)6888180.153846
0.0831.97E-10SGTC_1458k015-0034 6.3 μMChemDiv (Drug-like library)35989570.16
0.0822.51E-10SGTC_407eburnamonine 100.0 μMMiscellaneous31950.126984
0.0814.75E-10SGTC_13641653-0028 37.6 μMChemDiv (Drug-like library)57186520.08

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12490481-001510.2 μM0.5135144227577ChemDiv (Drug-like library)325.788844.18103
SGTC_2080521252412.4 μM0.46875260183Chembridge (Fragment library)221.639721.36113copper-dependent oxidative stress
SGTC_2130NSC 72.45 μM0.4687521448Chembridge (Fragment library)235.66631.89703copper-dependent oxidative stress
SGTC_230775389266.34 μM0.38235396582Chembridge (Fragment library)228.031621.77303
SGTC_3883',4'-dichloroisocoumarin16.7 μM0.3636361609Miscellaneous215.032862.75802
SGTC_501nsc-95397161 μM0.342857262093ICCB bioactive library310.388561.17126
SGTC_2781556727671.43 μM0.3404262853274Chembridge (Drug-like library)321.755542.10215
SGTC_2681menadione3.18 μM0.3333334055Miscellaneous172.180022.20402superoxide
SGTC_394menadione8.33 μM0.3333334055Miscellaneous172.180022.20402superoxide
SGTC_6120302-016722.4 μM0.333333235821ChemDiv (Drug-like library)287.654743.36704copper-dependent oxidative stress
SGTC_1036k216-021533.5 μM0.325622330ChemDiv (Drug-like library)266.681883.90803mitochondrial response to ROS