4023502

5-chloro-1-methylindole-2,3-dione

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2001
Screen concentration 57.7 μM
Source Chembridge (Fragment library)
PubChem CID 108941
SMILES CN1C2=C(C=C(C=C2)Cl)C(=O)C1=O
Standardized SMILES CN1C(=O)C(=O)c2cc(Cl)ccc12
Molecular weight 195.6024
ALogP 1.54
H-bond donor count 0
H-bond acceptor count 2
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.29
% growth inhibition (Hom. pool) 4.32


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 108941
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:4.38|P-value:5.89E-6|Clearance:0.08||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC20(YGL116W)|FD-Score:-4.26|P-value:1.04E-5|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:HEM12(YDR047W)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.21||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:4.92|P-value:4.35E-7|Clearance:0.14||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HYP2(YEL034W)|FD-Score:4.08|P-value:2.24E-5|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LAS17(YOR181W)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:PAM18(YLR008C)|FD-Score:-4.75|P-value:1.02E-6|Clearance:0||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:RFC2(YJR068W)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.19||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPB2(YOR151C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.16||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RRN11(YML043C)|FD-Score:-3.48|P-value:2.50E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RSC6(YCR052W)|FD-Score:13.7|P-value:6.30E-43|Clearance:7.97||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:3.37|P-value:3.77E-4|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC21(YNL287W)|FD-Score:-4.02|P-value:2.93E-5|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SFH1(YLR321C)|FD-Score:4.08|P-value:2.26E-5|Clearance:0.55||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:TOM40(YMR203W)|FD-Score:4.78|P-value:8.74E-7|Clearance:0.4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRS23(YDR246W)|FD-Score:-4.02|P-value:2.88E-5|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:YDL196W(YDL196W_d)|FD-Score:5.71|P-value:5.56E-9|Clearance:0.79||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGL074C(YGL074C_d)|FD-Score:4.3|P-value:8.59E-6|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:ARP7(YPR034W)|FD-Score:4.38|P-value:5.89E-6|Clearance:0.08||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC20(YGL116W)|FD-Score:-4.26|P-value:1.04E-5|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:HEM12(YDR047W)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.21||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:4.92|P-value:4.35E-7|Clearance:0.14||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HYP2(YEL034W)|FD-Score:4.08|P-value:2.24E-5|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LAS17(YOR181W)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:PAM18(YLR008C)|FD-Score:-4.75|P-value:1.02E-6|Clearance:0||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:RFC2(YJR068W)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.19||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPB2(YOR151C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.16||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RRN11(YML043C)|FD-Score:-3.48|P-value:2.50E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RSC6(YCR052W)|FD-Score:13.7|P-value:6.30E-43|Clearance:7.97||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:3.37|P-value:3.77E-4|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC21(YNL287W)|FD-Score:-4.02|P-value:2.93E-5|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SFH1(YLR321C)|FD-Score:4.08|P-value:2.26E-5|Clearance:0.55||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:TOM40(YMR203W)|FD-Score:4.78|P-value:8.74E-7|Clearance:0.4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRS23(YDR246W)|FD-Score:-4.02|P-value:2.88E-5|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:YDL196W(YDL196W_d)|FD-Score:5.71|P-value:5.56E-9|Clearance:0.79||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGL074C(YGL074C_d)|FD-Score:4.3|P-value:8.59E-6|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 108941
Download HOP data (tab-delimited text)  (excel)
Gene:ACK1(YDL203C)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:AIP1(YMR092C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ALG9(YNL219C)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:AVL9(YLR114C)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BIT61(YJL058C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BRP1(YGL007W_d)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BTN2(YGR142W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COS111(YBR203W)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:COX5B(YIL111W)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication Gene:DAP2(YHR028C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DBR1(YKL149C)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DDR2(YOL052C-A)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:FAA1(YOR317W)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FLC1(YPL221W)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FMP16(YDR070C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FMP23(YBR047W_p)|FD-Score:7.06|P-value:8.04E-13||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP25(YLR077W)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FMT1(YBL013W)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FYV12(YOR183W_p)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCR2(YNL199C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GLE2(YER107C)|FD-Score:-3.45|P-value:2.86E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GPM3(YOL056W)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GSH2(YOL049W)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:LAS21(YJL062W)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LYS14(YDR034C)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MCM22(YJR135C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MDM36(YPR083W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:NPC2(YDL046W)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OPI8(YKR035C_d)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PCD1(YLR151C)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PPM1(YDR435C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRS2(YER099C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PSK2(YOL045W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUS1(YPL212C)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:QCR10(YHR001W-A)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:QCR2(YPR191W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:RAD28(YDR030C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RGL1(YPL066W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIB4(YOL143C)|FD-Score:-3.82|P-value:6.76E-5||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RLF2(YPR018W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RNH70(YGR276C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPS0B(YLR048W)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RTF1(YGL244W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAP30(YMR263W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAY1(YGR263C)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SCD6(YPR129W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SIP18(YMR175W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SKP2(YNL311C)|FD-Score:-3.94|P-value:4.16E-5||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLY41(YOR307C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SOD1(YJR104C)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOD2(YHR008C)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SOV1(YMR066W)|FD-Score:5.41|P-value:3.16E-8||SGD DESC:Mitochondrial protein of unknown function Gene:SPO22(YIL073C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SSP1(YHR184W)|FD-Score:4.29|P-value:8.88E-6||SGD DESC:Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis Gene:SUE1(YPR151C)|FD-Score:5.13|P-value:1.42E-7||SGD DESC:Mitochondrial protein required for degradation of unstable forms of cytochrome c Gene:SUS1(YBR111W-A)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TKL1(YPR074C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMN3(YER113C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation Gene:TOK1(YJL093C)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TOS6(YNL300W_p)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:VMA3(YEL027W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VOA1(YGR106C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS27(YNR006W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YAP3(YHL009C)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBR238C(YBR238C)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR285W(YBR285W_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YBR292C(YBR292C_d)|FD-Score:-5.45|P-value:2.59E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YDR090C(YDR090C_p)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Putative protein of unknown function Gene:YDR199W(YDR199W_d)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR203W(YDR203W_d)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJR061W(YJR061W_p)|FD-Score:4.34|P-value:7.10E-6||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YLL007C(YLL007C_p)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR122C(YLR122C_d)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR244W(YMR244W_p)|FD-Score:-4.61|P-value:2.02E-6||SGD DESC:Putative protein of unknown function Gene:YOR283W(YOR283W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPL199C(YPL199C_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR109W(YPR109W_p)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:ZWF1(YNL241C)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:ACK1(YDL203C)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:AIP1(YMR092C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ALG9(YNL219C)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:AVL9(YLR114C)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BIT61(YJL058C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BRP1(YGL007W_d)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BTN2(YGR142W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COS111(YBR203W)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:COX5B(YIL111W)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication Gene:DAP2(YHR028C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DBR1(YKL149C)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DDR2(YOL052C-A)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:FAA1(YOR317W)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FLC1(YPL221W)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FMP16(YDR070C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FMP23(YBR047W_p)|FD-Score:7.06|P-value:8.04E-13||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP25(YLR077W)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FMT1(YBL013W)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FYV12(YOR183W_p)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCR2(YNL199C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GLE2(YER107C)|FD-Score:-3.45|P-value:2.86E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GPM3(YOL056W)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GSH2(YOL049W)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:LAS21(YJL062W)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LYS14(YDR034C)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MCM22(YJR135C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MDM36(YPR083W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:NPC2(YDL046W)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OPI8(YKR035C_d)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PCD1(YLR151C)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PPM1(YDR435C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRS2(YER099C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PSK2(YOL045W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUS1(YPL212C)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:QCR10(YHR001W-A)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:QCR2(YPR191W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:RAD28(YDR030C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RGL1(YPL066W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIB4(YOL143C)|FD-Score:-3.82|P-value:6.76E-5||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RLF2(YPR018W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RNH70(YGR276C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPS0B(YLR048W)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RTF1(YGL244W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAP30(YMR263W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAY1(YGR263C)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SCD6(YPR129W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SIP18(YMR175W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SKP2(YNL311C)|FD-Score:-3.94|P-value:4.16E-5||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLY41(YOR307C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SOD1(YJR104C)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOD2(YHR008C)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SOV1(YMR066W)|FD-Score:5.41|P-value:3.16E-8||SGD DESC:Mitochondrial protein of unknown function Gene:SPO22(YIL073C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SSP1(YHR184W)|FD-Score:4.29|P-value:8.88E-6||SGD DESC:Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis Gene:SUE1(YPR151C)|FD-Score:5.13|P-value:1.42E-7||SGD DESC:Mitochondrial protein required for degradation of unstable forms of cytochrome c Gene:SUS1(YBR111W-A)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TKL1(YPR074C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMN3(YER113C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation Gene:TOK1(YJL093C)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TOS6(YNL300W_p)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:VMA3(YEL027W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VOA1(YGR106C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS27(YNR006W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YAP3(YHL009C)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBR238C(YBR238C)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR285W(YBR285W_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YBR292C(YBR292C_d)|FD-Score:-5.45|P-value:2.59E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YDR090C(YDR090C_p)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Putative protein of unknown function Gene:YDR199W(YDR199W_d)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR203W(YDR203W_d)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJR061W(YJR061W_p)|FD-Score:4.34|P-value:7.10E-6||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YLL007C(YLL007C_p)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR122C(YLR122C_d)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR244W(YMR244W_p)|FD-Score:-4.61|P-value:2.02E-6||SGD DESC:Putative protein of unknown function Gene:YOR283W(YOR283W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPL199C(YPL199C_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPR109W(YPR109W_p)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:ZWF1(YNL241C)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCR052W13.706.30E-437.97RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YDL196W_d5.715.56E-90.79YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YDL205C4.924.35E-70.14HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YMR203W4.788.74E-70.40TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YPR034W4.385.89E-60.08ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YGL074C_d4.308.59E-60.22YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YEL034W4.082.24E-50.00HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YLR321C4.082.26E-50.55SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YOR151C3.532.09E-40.16RPB2RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
YFR037C3.373.77E-40.04RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YJR068W3.334.31E-40.19RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YDR047W3.148.32E-40.21HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YNL240C2.940.001650.16NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YBR038W2.780.002740.00CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YGR060W2.770.002770.02ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR047W_p7.068.04E-13FMP23_pPutative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBR203W5.842.67E-9COS111Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR066W5.413.16E-8SOV1Mitochondrial protein of unknown function
YPR151C5.131.42E-7SUE1Mitochondrial protein required for degradation of unstable forms of cytochrome c
YLR114C5.042.34E-7AVL9Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
YGL007W_d4.817.52E-7BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YEL027W4.542.85E-6VMA3Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis
YKL149C4.483.66E-6DBR1RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition
YJR061W_p4.347.10E-6YJR061W_pPutative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication
YHR184W4.298.88E-6SSP1Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis
YLL010C4.241.13E-5PSR1Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p
YNL241C4.141.76E-5ZWF1Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress
YIL073C4.121.89E-5SPO22Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis
YPR109W_p4.102.05E-5YPR109W_pPredicted membrane protein; dipoid deletion strain has high budding index
YMR038C4.092.18E-5CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2001
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1742.03E-41SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.16superoxide
0.1722.38E-40SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.151515heme biosynthesis & mitochondrial translocase
0.1673.75E-38SGTC_20024023503 131.0 μMChembridge (Fragment library)47368630.375mitochondrial processes
0.1648.57E-37SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.140351copper-dependent oxidative stress
0.1631.70E-36SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0666667heme biosynthesis & mitochondrial translocase
0.1604.48E-35SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.617647heme biosynthesis & mitochondrial translocase
0.1453.63E-29SGTC_20114012611 177.0 μMChembridge (Fragment library)20498870.0625mitochondrial processes
0.1441.49E-28SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.177778superoxide
0.1431.72E-28SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.185185mitochondrial processes
0.1382.01E-26SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.177778superoxide
0.1361.01E-25SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.103448heme biosynthesis & mitochondrial translocase
0.1351.53E-25SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.22heme biosynthesis & mitochondrial translocase
0.1337.42E-25SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.118644RSC & ERG11
0.1231.80E-21SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.116279
0.1226.19E-21SGTC_14813346-2004 36.0 μMChemDiv (Drug-like library)27705220.0821918

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20124023501200 μM0.6451613477460Chembridge (Fragment library)175.183961.36202
SGTC_23369001819198.65 μM0.6176472989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_1915st06710530.6 μM0.39393969751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_22667929956200 μM0.3863642967153Chembridge (Fragment library)238.670240.76912
SGTC_20024023503131 μM0.3754736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_349k015-001424 μM0.3404262196908ChemDiv (Drug-like library)350.594463.87202
SGTC_12690831-07963.05 μM0.340426927758ChemDiv (Drug-like library)268.697763.1170160S ribosome export
SGTC_10483161-030024.5 μM0.3181822165101ChemDiv (Drug-like library)266.090722.24102
SGTC_2919797818846.38 μM0.3148152980291Chembridge (Drug-like library)331.750362.26224
SGTC_1028k015-00294.8 μM0.3137264588309ChemDiv (Drug-like library)351.141023.68304