4023503

1-ethyl-5-fluoroindole-2,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2002
Screen concentration 131.0 μM
Source Chembridge (Fragment library)
PubChem CID 4736863
SMILES CCN1C2=C(C=C(C=C2)F)C(=O)C1=O
Standardized SMILES CCN1C(=O)C(=O)c2cc(F)ccc12
Molecular weight 193.1744
ALogP 1.43
H-bond donor count 0
H-bond acceptor count 3
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.61
% growth inhibition (Hom. pool) 4.32


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4736863
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:-5.48|P-value:2.07E-8|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:AME1(YBR211C)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.1||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP7(YPR034W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ASA1(YPR085C)|FD-Score:-3.16|P-value:7.80E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CAB5(YDR196C)|FD-Score:3.81|P-value:7.04E-5|Clearance:0.12||SGD DESC:Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies Gene:CWC24(YLR323C)|FD-Score:-3.13|P-value:8.79E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DHR2(YKL078W)|FD-Score:3.9|P-value:4.80E-5|Clearance:0.09||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FCF1(YDR339C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.04||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:FCF2(YLR051C)|FD-Score:-4.15|P-value:1.64E-5|Clearance:0||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GPI18(YBR004C)|FD-Score:4.07|P-value:2.38E-5|Clearance:0.13||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:GUS1(YGL245W)|FD-Score:4.53|P-value:2.99E-6|Clearance:0.16||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:MCM7(YBR202W)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.1||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NOG1(YPL093W)|FD-Score:3.93|P-value:4.17E-5|Clearance:0.03||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP14(YDL148C)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.01||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP4(YPL043W)|FD-Score:-3.54|P-value:2.04E-4|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:PDS1(YDR113C)|FD-Score:-4.31|P-value:8.24E-6|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP9(YDL030W)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.08||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PSF1(YDR013W)|FD-Score:-3.97|P-value:3.64E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RER2(YBR002C)|FD-Score:5.94|P-value:1.41E-9|Clearance:0.6||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC2(YJR068W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.03||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPL5(YPL131W)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RRP4(YHR069C)|FD-Score:8.17|P-value:1.51E-16|Clearance:2.23||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC6(YCR052W)|FD-Score:16.5|P-value:8.89E-62|Clearance:8.37||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC16(YPL085W)|FD-Score:-3.72|P-value:9.85E-5|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEC9(YGR009C)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.12||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SEN1(YLR430W)|FD-Score:4.37|P-value:6.22E-6|Clearance:0.3||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SMC4(YLR086W)|FD-Score:-4.07|P-value:2.34E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SMD3(YLR147C)|FD-Score:3.09|P-value:9.87E-4|Clearance:0.01||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SRB7(YDR308C)|FD-Score:3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:TOM40(YMR203W)|FD-Score:5.35|P-value:4.52E-8|Clearance:0.82||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:URA6(YKL024C)|FD-Score:-3.99|P-value:3.27E-5|Clearance:0||SGD DESC:Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) Gene:USB1(YLR132C)|FD-Score:3.36|P-value:3.83E-4|Clearance:0.01||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:YSH1(YLR277C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.05||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:ALG14(YBR070C)|FD-Score:-5.48|P-value:2.07E-8|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:AME1(YBR211C)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.1||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP7(YPR034W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ASA1(YPR085C)|FD-Score:-3.16|P-value:7.80E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CAB5(YDR196C)|FD-Score:3.81|P-value:7.04E-5|Clearance:0.12||SGD DESC:Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies Gene:CWC24(YLR323C)|FD-Score:-3.13|P-value:8.79E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DHR2(YKL078W)|FD-Score:3.9|P-value:4.80E-5|Clearance:0.09||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FCF1(YDR339C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.04||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:FCF2(YLR051C)|FD-Score:-4.15|P-value:1.64E-5|Clearance:0||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GPI18(YBR004C)|FD-Score:4.07|P-value:2.38E-5|Clearance:0.13||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:GUS1(YGL245W)|FD-Score:4.53|P-value:2.99E-6|Clearance:0.16||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:MCM7(YBR202W)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.1||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NOG1(YPL093W)|FD-Score:3.93|P-value:4.17E-5|Clearance:0.03||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP14(YDL148C)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.01||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP4(YPL043W)|FD-Score:-3.54|P-value:2.04E-4|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:PDS1(YDR113C)|FD-Score:-4.31|P-value:8.24E-6|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP9(YDL030W)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.08||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PSF1(YDR013W)|FD-Score:-3.97|P-value:3.64E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RER2(YBR002C)|FD-Score:5.94|P-value:1.41E-9|Clearance:0.6||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC2(YJR068W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.03||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPL5(YPL131W)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RRP4(YHR069C)|FD-Score:8.17|P-value:1.51E-16|Clearance:2.23||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC6(YCR052W)|FD-Score:16.5|P-value:8.89E-62|Clearance:8.37||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC16(YPL085W)|FD-Score:-3.72|P-value:9.85E-5|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEC9(YGR009C)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.12||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SEN1(YLR430W)|FD-Score:4.37|P-value:6.22E-6|Clearance:0.3||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SMC4(YLR086W)|FD-Score:-4.07|P-value:2.34E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SMD3(YLR147C)|FD-Score:3.09|P-value:9.87E-4|Clearance:0.01||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SRB7(YDR308C)|FD-Score:3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:TOM40(YMR203W)|FD-Score:5.35|P-value:4.52E-8|Clearance:0.82||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:URA6(YKL024C)|FD-Score:-3.99|P-value:3.27E-5|Clearance:0||SGD DESC:Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) Gene:USB1(YLR132C)|FD-Score:3.36|P-value:3.83E-4|Clearance:0.01||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:YSH1(YLR277C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.05||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4736863
Download HOP data (tab-delimited text)  (excel)
Gene:AGX1(YFL030W)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:APC9(YLR102C)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ARI1(YGL157W)|FD-Score:5.33|P-value:4.85E-8||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARO1(YDR127W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ASF1(YJL115W)|FD-Score:-4.79|P-value:8.52E-7||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:BNA1(YJR025C)|FD-Score:-4.41|P-value:5.07E-6||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CBP1(YJL209W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CBT1(YKL208W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CNL1(YDR357C_p)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:COQ10(YOL008W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COX7(YMR256C)|FD-Score:9.29|P-value:7.62E-21||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTK3(YML112W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DXO1(YDR370C_p)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:ECM5(YMR176W)|FD-Score:-6.03|P-value:8.12E-10||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:FIG2(YCR089W)|FD-Score:4.02|P-value:2.87E-5||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FMP23(YBR047W_p)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMT1(YBL013W)|FD-Score:7.51|P-value:2.90E-14||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FPR1(YNL135C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GCN2(YDR283C)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GDE1(YPL110C)|FD-Score:6.8|P-value:5.10E-12||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GLR1(YPL091W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GLT1(YDL171C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GSH1(YJL101C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HSP78(YDR258C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:HXK2(YGL253W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:HXT12(YIL170W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:ILV6(YCL009C)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IRC15(YPL017C)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:IRC24(YIR036C_p)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:KCC4(YCL024W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:KSS1(YGR040W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LEU9(YOR108W)|FD-Score:-4.38|P-value:5.96E-6||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MAM3(YOL060C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MET5(YJR137C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine Gene:MGR3(YMR115W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MNE1(YOR350C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MPP6(YNR024W)|FD-Score:4.88|P-value:5.26E-7||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MTH1(YDR277C)|FD-Score:6.29|P-value:1.55E-10||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:NTE1(YML059C)|FD-Score:4.31|P-value:8.32E-6||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:NUM1(YDR150W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OPI8(YKR035C_d)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OTU1(YFL044C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PEX22(YAL055W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PIN3(YPR154W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PMT3(YOR321W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PPQ1(YPL179W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:QDR3(YBR043C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RAD28(YDR030C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RER1(YCL001W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:RPP1B(YDL130W)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS0A(YGR214W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS10A(YOR293W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTA1(YGR213C)|FD-Score:8.18|P-value:1.43E-16||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SGF73(YGL066W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SML1(YML058W)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase Gene:SNF7(YLR025W)|FD-Score:-4.32|P-value:7.70E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNT2(YGL131C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SSF2(YDR312W)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:STE50(YCL032W)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUE1(YPR151C)|FD-Score:5.84|P-value:2.62E-9||SGD DESC:Mitochondrial protein required for degradation of unstable forms of cytochrome c Gene:SUL2(YLR092W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWD1(YAR003W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SWM1(YDR260C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TRM7(YBR061C)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:UBA4(YHR111W)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBC5(YDR059C)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:VHR1(YIL056W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication Gene:VPS65(YLR322W_d)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YBR277C(YBR277C_d)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCF1(YDR135C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YCK3(YER123W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR061W(YCR061W_p)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDL176W(YDL176W)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR133C(YDR133C_d)|FD-Score:4.4|P-value:5.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR374C(YDR374C_p)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function Gene:YER067C-A(YER067C-A_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YGR018C(YGR018C_p)|FD-Score:5.61|P-value:9.84E-9||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YGR042W(YGR042W_p)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YGR107W(YGR107W_d)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR283C(YGR283C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YHR175W-A(YHR175W-A_p)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJR079W(YJR079W_p)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YJR107W(YJR107W_p)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Putative lipase Gene:YKL030W(YKL030W_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL169C(YKL169C_d)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR177W(YLR177W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR460C(YLR460C_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR173W-A(YMR173W-A_d)|FD-Score:7.62|P-value:1.29E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL013C(YNL013C_d)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Gene:YNL092W(YNL092W_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNL140C(YNL140C_p)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOR053W(YOR053W_d)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPR117W(YPR117W_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function Gene:YPT32(YGL210W)|FD-Score:4.49|P-value:3.64E-6||SGD DESC:Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication Gene:ZWF1(YNL241C)|FD-Score:6.05|P-value:7.07E-10||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AGX1(YFL030W)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:APC9(YLR102C)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ARI1(YGL157W)|FD-Score:5.33|P-value:4.85E-8||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARO1(YDR127W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ASF1(YJL115W)|FD-Score:-4.79|P-value:8.52E-7||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:BNA1(YJR025C)|FD-Score:-4.41|P-value:5.07E-6||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CBP1(YJL209W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CBT1(YKL208W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CNL1(YDR357C_p)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:COQ10(YOL008W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COX7(YMR256C)|FD-Score:9.29|P-value:7.62E-21||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTK3(YML112W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DXO1(YDR370C_p)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:ECM5(YMR176W)|FD-Score:-6.03|P-value:8.12E-10||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:FIG2(YCR089W)|FD-Score:4.02|P-value:2.87E-5||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FMP23(YBR047W_p)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMT1(YBL013W)|FD-Score:7.51|P-value:2.90E-14||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FPR1(YNL135C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GCN2(YDR283C)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GDE1(YPL110C)|FD-Score:6.8|P-value:5.10E-12||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GLR1(YPL091W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GLT1(YDL171C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GSH1(YJL101C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HSP78(YDR258C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:HXK2(YGL253W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:HXT12(YIL170W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:ILV6(YCL009C)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IRC15(YPL017C)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:IRC24(YIR036C_p)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:KCC4(YCL024W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:KSS1(YGR040W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LEU9(YOR108W)|FD-Score:-4.38|P-value:5.96E-6||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MAM3(YOL060C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MET5(YJR137C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine Gene:MGR3(YMR115W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MNE1(YOR350C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MPP6(YNR024W)|FD-Score:4.88|P-value:5.26E-7||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MTH1(YDR277C)|FD-Score:6.29|P-value:1.55E-10||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:NTE1(YML059C)|FD-Score:4.31|P-value:8.32E-6||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:NUM1(YDR150W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OPI8(YKR035C_d)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OTU1(YFL044C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PEX22(YAL055W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PIN3(YPR154W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PMT3(YOR321W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PPQ1(YPL179W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:QDR3(YBR043C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RAD28(YDR030C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RER1(YCL001W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:RPP1B(YDL130W)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS0A(YGR214W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS10A(YOR293W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTA1(YGR213C)|FD-Score:8.18|P-value:1.43E-16||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SGF73(YGL066W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SML1(YML058W)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase Gene:SNF7(YLR025W)|FD-Score:-4.32|P-value:7.70E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNT2(YGL131C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SSF2(YDR312W)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:STE50(YCL032W)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUE1(YPR151C)|FD-Score:5.84|P-value:2.62E-9||SGD DESC:Mitochondrial protein required for degradation of unstable forms of cytochrome c Gene:SUL2(YLR092W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWD1(YAR003W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SWM1(YDR260C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TRM7(YBR061C)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:UBA4(YHR111W)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBC5(YDR059C)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:VHR1(YIL056W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication Gene:VPS65(YLR322W_d)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YBR277C(YBR277C_d)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCF1(YDR135C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YCK3(YER123W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR061W(YCR061W_p)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDL176W(YDL176W)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR133C(YDR133C_d)|FD-Score:4.4|P-value:5.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR374C(YDR374C_p)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function Gene:YER067C-A(YER067C-A_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YGR018C(YGR018C_p)|FD-Score:5.61|P-value:9.84E-9||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YGR042W(YGR042W_p)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YGR107W(YGR107W_d)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR283C(YGR283C)|FD-Score:3.87|P-value:5.37E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YHR175W-A(YHR175W-A_p)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJR079W(YJR079W_p)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YJR107W(YJR107W_p)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Putative lipase Gene:YKL030W(YKL030W_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL169C(YKL169C_d)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR177W(YLR177W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR460C(YLR460C_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR173W-A(YMR173W-A_d)|FD-Score:7.62|P-value:1.29E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL013C(YNL013C_d)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Gene:YNL092W(YNL092W_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNL140C(YNL140C_p)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOR053W(YOR053W_d)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPR117W(YPR117W_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function Gene:YPT32(YGL210W)|FD-Score:4.49|P-value:3.64E-6||SGD DESC:Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication Gene:ZWF1(YNL241C)|FD-Score:6.05|P-value:7.07E-10||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCR052W16.508.89E-628.37RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YHR069C8.171.51E-162.23RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YBR002C5.941.41E-90.60RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YMR203W5.354.52E-80.82TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YGL245W4.532.99E-60.16GUS1Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
YLR430W4.376.22E-60.30SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YBR004C4.072.38E-50.13GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YPL093W3.934.17E-50.03NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YKL078W3.904.80E-50.09DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YDR196C3.817.04E-50.12CAB5Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies
YDR339C3.691.14E-40.04FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YBR211C3.641.34E-40.10AME1Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
YDR308C3.541.98E-40.00SRB7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
YJR068W3.542.01E-40.03RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YPR034W3.512.27E-40.01ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR256C9.297.62E-21COX7Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YGR213C8.181.43E-16RTA1Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
YMR173W-A_d7.621.29E-14YMR173W-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W
YBL013W7.512.90E-14FMT1Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
YPL110C6.805.10E-12GDE1Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
YDR277C6.291.55E-10MTH1Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation
YNL241C6.057.07E-10ZWF1Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress
YPR151C5.842.62E-9SUE1Mitochondrial protein required for degradation of unstable forms of cytochrome c
YGR018C_p5.619.84E-9YGR018C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W
YGL157W5.334.85E-8ARI1NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
YGR042W_p5.141.39E-7YGR042W_pProtein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
YDR312W5.101.71E-7SSF2Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
YOL117W5.042.35E-7RRI2Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
YNR024W4.885.26E-7MPP6Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes
YBR277C_d4.751.03E-6YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W

GO enrichment analysis for SGTC_2002
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1673.75E-38SGTC_20014023502 57.7 μMChembridge (Fragment library)1089410.375mitochondrial processes
0.0976.57E-14SGTC_493zm226600 134.0 μMICCB bioactive library52400980.101695mitochondrial processes
0.0944.14E-13SGTC_22287354201 200.0 μMChembridge (Fragment library)7534510.105263endomembrane recycling
0.0938.42E-13SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.1
0.0921.40E-12SGTC_1992st073643 23.3 μMTimTec (Natural product derivative library)31612920.16129mitochondrial processes
0.0898.13E-12SGTC_5203584-0023 682.0 μMChemDiv (Drug-like library)13407900.135593
0.0864.34E-11SGTC_13541498-1681 81.0 μMChemDiv (Drug-like library)57542430.171429
0.0822.31E-10SGTC_462ag-879 158.0 μMICCB bioactive library68096540.0677966
0.0813.73E-10SGTC_22567954252 200.0 μMChembridge (Fragment library)29729200.0701754mitochondrial processes
0.0741.15E-8SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.078125RSC & ERG11
0.0731.68E-8SGTC_9211561-0023 372.0 μMChemDiv (Drug-like library)240508820.0857143
0.0723.24E-8SGTC_2516gossypol 71.5 μMTimTec (Pure natural product library)35030.0877193
0.0713.97E-8SGTC_14813346-2004 36.0 μMChemDiv (Drug-like library)27705220.123288
0.0714.79E-8SGTC_31009120536 49.5 μMChembridge (Drug-like library)164211960.057971
0.0706.30E-8SGTC_1567st069303 68.4 μMTimTec (Pure natural product library)55813190.0833333mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_23369001819198.65 μM0.6571432989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_169k015-001716.03 μM0.523811840002ChemDiv (Drug-like library)334.1398633.41303
SGTC_1458k015-00346.33 μM0.4883723598957ChemDiv (Drug-like library)352.1303263.61904
SGTC_20605238662140 μM0.447368790130Chembridge (Fragment library)195.1472430.5114
SGTC_10483161-030024.5 μM0.4186052165101ChemDiv (Drug-like library)266.090722.24102
SGTC_20225136654200 μM0.3902441713771Chembridge (Fragment library)204.225180.99703
SGTC_295k015-002731 μM0.3877553887424ChemDiv (Drug-like library)296.277482.8404PDR1
SGTC_20124023501200 μM0.3846153477460Chembridge (Fragment library)175.183961.36202
SGTC_349k015-001424 μM0.3752196908ChemDiv (Drug-like library)350.594463.87202
SGTC_2001402350257.66 μM0.375108941Chembridge (Fragment library)195.602441.5402mitochondrial processes