4023501

1,5-dimethylindole-2,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2012
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 3477460
SMILES CC1=CC2=C(C=C1)N(C(=O)C2=O)C
Standardized SMILES CN1C(=O)C(=O)c2cc(C)ccc12
Molecular weight 175.184
ALogP 1.36
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.18
% growth inhibition (Hom. pool) 1.14


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3477460
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:-4.45|P-value:4.34E-6|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ARC19(YKL013C)|FD-Score:4.88|P-value:5.35E-7|Clearance:0.39||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:AVO1(YOL078W)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.16||SGD DESC:Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BBP1(YPL255W)|FD-Score:-3.83|P-value:6.34E-5|Clearance:0||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:DHR2(YKL078W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.22||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DUT1(YBR252W)|FD-Score:3.37|P-value:3.76E-4|Clearance:0.04||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ERG9(YHR190W)|FD-Score:-5.65|P-value:8.01E-9|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:GAB1(YLR459W)|FD-Score:4.88|P-value:5.28E-7|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:MCM10(YIL150C)|FD-Score:6.01|P-value:9.46E-10|Clearance:0.84||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:NAB2(YGL122C)|FD-Score:7.16|P-value:3.99E-13|Clearance:0.96||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NAF1(YNL124W)|FD-Score:-4.03|P-value:2.74E-5|Clearance:0||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:ORC2(YBR060C)|FD-Score:4.48|P-value:3.67E-6|Clearance:0.26||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:PRP9(YDL030W)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.28||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PUP1(YOR157C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.01||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RAD53(YPL153C)|FD-Score:4.21|P-value:1.27E-5|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RNA14(YMR061W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC10(YHR143W-A)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:SEC13(YLR208W)|FD-Score:3.86|P-value:5.67E-5|Clearance:0.04||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC23(YPR181C)|FD-Score:-5.41|P-value:3.13E-8|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SES1(YDR023W)|FD-Score:3.93|P-value:4.21E-5|Clearance:0.07||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SPC97(YHR172W)|FD-Score:-4.49|P-value:3.62E-6|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SSY1(YDR160W)|FD-Score:5.17|P-value:1.18E-7|Clearance:0.29||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:SUB2(YDL084W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.06||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TAF1(YGR274C)|FD-Score:3.26|P-value:5.66E-4|Clearance:0.07||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TAF4(YMR005W)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.01||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TFG1(YGR186W)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.04||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TID3(YIL144W)|FD-Score:-3.51|P-value:2.28E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UTP10(YJL109C)|FD-Score:6.2|P-value:2.80E-10|Clearance:0.19||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YLR339C(YLR339C_d)|FD-Score:3.82|P-value:6.78E-5|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:YNL114C(YNL114C_d)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YOR203W(YOR203W_d)|FD-Score:3.74|P-value:9.12E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:ALG14(YBR070C)|FD-Score:-4.45|P-value:4.34E-6|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ARC19(YKL013C)|FD-Score:4.88|P-value:5.35E-7|Clearance:0.39||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:AVO1(YOL078W)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.16||SGD DESC:Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BBP1(YPL255W)|FD-Score:-3.83|P-value:6.34E-5|Clearance:0||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:DHR2(YKL078W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.22||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DUT1(YBR252W)|FD-Score:3.37|P-value:3.76E-4|Clearance:0.04||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ERG9(YHR190W)|FD-Score:-5.65|P-value:8.01E-9|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:GAB1(YLR459W)|FD-Score:4.88|P-value:5.28E-7|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:MCM10(YIL150C)|FD-Score:6.01|P-value:9.46E-10|Clearance:0.84||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:NAB2(YGL122C)|FD-Score:7.16|P-value:3.99E-13|Clearance:0.96||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NAF1(YNL124W)|FD-Score:-4.03|P-value:2.74E-5|Clearance:0||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:ORC2(YBR060C)|FD-Score:4.48|P-value:3.67E-6|Clearance:0.26||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:PRP9(YDL030W)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.28||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PUP1(YOR157C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.01||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RAD53(YPL153C)|FD-Score:4.21|P-value:1.27E-5|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RNA14(YMR061W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC10(YHR143W-A)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:SEC13(YLR208W)|FD-Score:3.86|P-value:5.67E-5|Clearance:0.04||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC23(YPR181C)|FD-Score:-5.41|P-value:3.13E-8|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SES1(YDR023W)|FD-Score:3.93|P-value:4.21E-5|Clearance:0.07||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SPC97(YHR172W)|FD-Score:-4.49|P-value:3.62E-6|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SSY1(YDR160W)|FD-Score:5.17|P-value:1.18E-7|Clearance:0.29||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:SUB2(YDL084W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.06||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TAF1(YGR274C)|FD-Score:3.26|P-value:5.66E-4|Clearance:0.07||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TAF4(YMR005W)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.01||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TFG1(YGR186W)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.04||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TID3(YIL144W)|FD-Score:-3.51|P-value:2.28E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UTP10(YJL109C)|FD-Score:6.2|P-value:2.80E-10|Clearance:0.19||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YLR339C(YLR339C_d)|FD-Score:3.82|P-value:6.78E-5|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:YNL114C(YNL114C_d)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YOR203W(YOR203W_d)|FD-Score:3.74|P-value:9.12E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3477460
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:4.97|P-value:3.39E-7||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADH6(YMR318C)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:4.97|P-value:3.38E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARF3(YOR094W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ARO4(YBR249C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG21(YPL100W)|FD-Score:6.84|P-value:3.86E-12||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:BUD17(YNR027W)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CDC26(YFR036W)|FD-Score:4.89|P-value:4.96E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC73(YLR418C)|FD-Score:7.59|P-value:1.65E-14||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLN2(YPL256C)|FD-Score:4.86|P-value:6.00E-7||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COA1(YIL157C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COG6(YNL041C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCG1(YIR030C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DDI1(YER143W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DYN3(YMR299C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FAR7(YFR008W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FMP16(YDR070C_p)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FMP46(YKR049C)|FD-Score:-4.85|P-value:6.16E-7||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP48(YGR052W_p)|FD-Score:-4.36|P-value:6.36E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FSH3(YOR280C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GIS2(YNL255C)|FD-Score:-4.48|P-value:3.80E-6||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GIS3(YLR094C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function Gene:GLE2(YER107C)|FD-Score:6.13|P-value:4.50E-10||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPM3(YOL056W)|FD-Score:-3.99|P-value:3.34E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HIS3(YOR202W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:HNT1(YDL125C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IZH2(YOL002C)|FD-Score:-3.84|P-value:6.10E-5||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KAP104(YBR017C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:KEL2(YGR238C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LIN1(YHR156C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MAC1(YMR021C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCT1(YOR221C)|FD-Score:5.83|P-value:2.69E-9||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MKC7(YDR144C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MRP49(YKL167C)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSY1(YPL097W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:OCA1(YNL099C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OPI9(YLR338W_d)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PCL9(YDL179W)|FD-Score:-4.41|P-value:5.27E-6||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PDR1(YGL013C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PET122(YER153C)|FD-Score:4.95|P-value:3.68E-7||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PGC1(YPL206C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PIM1(YBL022C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PRK1(YIL095W)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRS2(YER099C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:RGD2(YFL047W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL14B(YHL001W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL31A(YDL075W)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPS10A(YOR293W)|FD-Score:-3.98|P-value:3.41E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RTR2(YDR066C_p)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAP4(YGL229C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SLZ1(YNL196C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNA2(YDR525W-A)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOL2(YCR073W-A)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SRO7(YPR032W)|FD-Score:-6.15|P-value:3.78E-10||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SRP40(YKR092C)|FD-Score:5.37|P-value:3.89E-8||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSA2(YLL024C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STE20(YHL007C)|FD-Score:-3.74|P-value:9.38E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SYS1(YJL004C)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THI20(YOL055C)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Gene:TMA7(YLR262C-A)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TOK1(YJL093C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:VPH2(YKL119C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS1(YKR001C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS3(YDR495C)|FD-Score:10.4|P-value:1.49E-25||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:XBP1(YIL101C)|FD-Score:6.15|P-value:3.87E-10||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR226C(YBR226C_d)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL001W-A(YCL001W-A_p)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR006C(YCR006C_d)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR075W-A(YCR075W-A_p)|FD-Score:4.84|P-value:6.37E-7||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDR248C(YDR248C_p)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YGL214W(YGL214W_d)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR107W(YGR107W_d)|FD-Score:-4.27|P-value:9.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR273C(YGR273C_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHL015W-A(YHL015W-A_p)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Putative protein of unknown function Gene:YKL169C(YKL169C_d)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR040C(YLR040C_p)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR255C(YLR255C_d)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YLR460C(YLR460C_p)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR158W-B(YMR158W-B_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YMR173W-A(YMR173W-A_d)|FD-Score:4.09|P-value:2.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR226C(YMR226C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YNK1(YKL067W)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YNR005C(YNR005C_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR065C(YNR065C_p)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR022C(YOR022C_p)|FD-Score:3.73|P-value:9.65E-5||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPK1(YKL126W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL034W(YPL034W_p)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPT11(YNL304W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:YPT53(YNL093W)|FD-Score:8.02|P-value:5.32E-16||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ZWF1(YNL241C)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:ACM1(YPL267W)|FD-Score:4.97|P-value:3.39E-7||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADH6(YMR318C)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:4.97|P-value:3.38E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARF3(YOR094W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ARO4(YBR249C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG21(YPL100W)|FD-Score:6.84|P-value:3.86E-12||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:BUD17(YNR027W)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CDC26(YFR036W)|FD-Score:4.89|P-value:4.96E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC73(YLR418C)|FD-Score:7.59|P-value:1.65E-14||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLN2(YPL256C)|FD-Score:4.86|P-value:6.00E-7||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COA1(YIL157C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COG6(YNL041C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCG1(YIR030C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DDI1(YER143W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DYN3(YMR299C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FAR7(YFR008W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FMP16(YDR070C_p)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FMP46(YKR049C)|FD-Score:-4.85|P-value:6.16E-7||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP48(YGR052W_p)|FD-Score:-4.36|P-value:6.36E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FSH3(YOR280C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GIS2(YNL255C)|FD-Score:-4.48|P-value:3.80E-6||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GIS3(YLR094C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function Gene:GLE2(YER107C)|FD-Score:6.13|P-value:4.50E-10||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPM3(YOL056W)|FD-Score:-3.99|P-value:3.34E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HIS3(YOR202W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:HNT1(YDL125C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IZH2(YOL002C)|FD-Score:-3.84|P-value:6.10E-5||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KAP104(YBR017C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:KEL2(YGR238C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LIN1(YHR156C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MAC1(YMR021C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCT1(YOR221C)|FD-Score:5.83|P-value:2.69E-9||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MKC7(YDR144C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MRP49(YKL167C)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSY1(YPL097W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:OCA1(YNL099C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OPI9(YLR338W_d)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PCL9(YDL179W)|FD-Score:-4.41|P-value:5.27E-6||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PDR1(YGL013C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PET122(YER153C)|FD-Score:4.95|P-value:3.68E-7||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PGC1(YPL206C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PIM1(YBL022C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PRK1(YIL095W)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRS2(YER099C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:RGD2(YFL047W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL14B(YHL001W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL31A(YDL075W)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPS10A(YOR293W)|FD-Score:-3.98|P-value:3.41E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RTR2(YDR066C_p)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAP4(YGL229C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SLZ1(YNL196C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNA2(YDR525W-A)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOL2(YCR073W-A)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SRO7(YPR032W)|FD-Score:-6.15|P-value:3.78E-10||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SRP40(YKR092C)|FD-Score:5.37|P-value:3.89E-8||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSA2(YLL024C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STE20(YHL007C)|FD-Score:-3.74|P-value:9.38E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SYS1(YJL004C)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THI20(YOL055C)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Gene:TMA7(YLR262C-A)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TOK1(YJL093C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:VPH2(YKL119C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS1(YKR001C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS3(YDR495C)|FD-Score:10.4|P-value:1.49E-25||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:XBP1(YIL101C)|FD-Score:6.15|P-value:3.87E-10||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR226C(YBR226C_d)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL001W-A(YCL001W-A_p)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR006C(YCR006C_d)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR075W-A(YCR075W-A_p)|FD-Score:4.84|P-value:6.37E-7||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDR248C(YDR248C_p)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 Gene:YGL214W(YGL214W_d)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR107W(YGR107W_d)|FD-Score:-4.27|P-value:9.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR273C(YGR273C_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHL015W-A(YHL015W-A_p)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Putative protein of unknown function Gene:YKL169C(YKL169C_d)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR040C(YLR040C_p)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR255C(YLR255C_d)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YLR460C(YLR460C_p)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR158W-B(YMR158W-B_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YMR173W-A(YMR173W-A_d)|FD-Score:4.09|P-value:2.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR226C(YMR226C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YNK1(YKL067W)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YNR005C(YNR005C_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR065C(YNR065C_p)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR022C(YOR022C_p)|FD-Score:3.73|P-value:9.65E-5||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPK1(YKL126W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL034W(YPL034W_p)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPT11(YNL304W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:YPT53(YNL093W)|FD-Score:8.02|P-value:5.32E-16||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ZWF1(YNL241C)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL122C7.163.99E-130.96NAB2Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress
YJL109C6.202.80E-100.19UTP10Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
YIL150C6.019.46E-100.84MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YDR160W5.171.18E-70.29SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YLR459W4.885.28E-70.00GAB1GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
YKL013C4.885.35E-70.40ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YBR060C4.483.67E-60.26ORC2Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
YOR157C4.231.19E-50.01PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YPL153C4.211.27E-50.00RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YDL030W4.211.28E-50.28PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YDR023W3.934.21E-50.07SES1Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
YLR208W3.865.67E-50.04SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YLR339C_d3.826.78E-50.07YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
YOR203W_d3.749.12E-50.09YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation
YDL084W3.651.31E-40.06SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR495C10.401.49E-25VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YNL093W8.025.32E-16YPT53Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
YLR418C7.591.65E-14CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YPL100W6.843.86E-12ATG21Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein
YIL101C6.153.87E-10XBP1Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YER107C6.134.50E-10GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YOR221C5.832.69E-9MCT1Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
YKR092C5.373.89E-8SRP40Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
YDR070C_p5.171.15E-7FMP16_pProtein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
YIL095W5.101.69E-7PRK1Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication
YGL071W4.973.38E-7AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YPL267W4.973.39E-7ACM1Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein
YER153C4.953.68E-7PET122Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
YFR036W4.894.96E-7CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YPL256C4.866.00E-7CLN2G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)

GO enrichment analysis for SGTC_2012
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0832.16E-10SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.113208
0.0741.44E-8SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.137255
0.0722.60E-8SGTC_24435493264 126.7 μMMiscellaneous53400550.0517241
0.0723.03E-8SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0731707
0.0713.85E-8SGTC_1536cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid 61.9 μMTimTec (Pure natural product library)665350.0757576
0.0672.92E-7SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.0655738TSC3-RPN4
0.0663.49E-7SGTC_6383970-0837 4.1 μMChemDiv (Drug-like library)41184510.145455
0.0663.53E-7SGTC_1483253-0744 288.0 μMChemDiv (Drug-like library)68936550.116667
0.0654.76E-7SGTC_21355326629 150.4 μMChembridge (Fragment library)50200770.136364RPP1 & pyrimidine depletion
0.0631.40E-6SGTC_12790874-0590 65.2 μMChemDiv (Drug-like library)35230740.113208
0.0621.81E-6SGTC_117lucigenin 22.1 μMChemDiv (Drug-like library)651000.102564redox potentiating
0.0603.76E-6SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.102941RSC & ERG11
0.0604.45E-6SGTC_24615755302 26.1 μMMiscellaneous27889040.0967742
0.0595.58E-6SGTC_14163966-0321 25.3 μMChemDiv (Drug-like library)5825300.177778
0.0595.85E-6SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.0819672PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2001402350257.66 μM0.645161108941Chembridge (Fragment library)195.602441.5402mitochondrial processes
SGTC_1915st06710530.6 μM0.4062569751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_20405100729123 μM0.411840Chembridge (Fragment library)161.157381.15612
SGTC_295k015-002731 μM0.3913043887424ChemDiv (Drug-like library)296.277482.8404PDR1
SGTC_20024023503131 μM0.3846154736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_23369001819198.65 μM0.3846152989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_1686st019653115.5 μM0.35135197252TimTec (Natural product derivative library)173.211141.90401mitochondrial processes
SGTC_7414582-0002126 μM0.3414632912688ChemDiv (Drug-like library)241.28512.61202
SGTC_10483161-030024.5 μM0.3255812165101ChemDiv (Drug-like library)266.090722.24102
SGTC_14790549-0158134 μM0.3191495440202ChemDiv (Drug-like library)356.17023.19913