4013930

1-adamantylmethanol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2014
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 64556
SMILES C1C2CC3CC1CC(C2)(C3)CO
Standardized SMILES OCC12CC3CC(CC(C3)C1)C2
Molecular weight 166.26
ALogP 2.12
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.29
% growth inhibition (Hom. pool) 7.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 64556
Download HIP data (tab-delimited text)  (excel)
Gene:CBF2(YGR140W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.09||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CEF1(YMR213W)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.07||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:GPI11(YDR302W)|FD-Score:-6.7|P-value:1.08E-11|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:IPL1(YPL209C)|FD-Score:4.48|P-value:3.73E-6|Clearance:0.5||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:IRR1(YIL026C)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.08||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NOC2(YOR206W)|FD-Score:3.79|P-value:7.50E-5|Clearance:0.22||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:POL31(YJR006W)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.02||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:PRE1(YER012W)|FD-Score:5.48|P-value:2.16E-8|Clearance:1||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:QRI1(YDL103C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.1||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:3.29|P-value:5.06E-4|Clearance:0.16||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT2(YDL007W)|FD-Score:3.98|P-value:3.47E-5|Clearance:0.11||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:STH1(YIL126W)|FD-Score:-4.09|P-value:2.12E-5|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TAF7(YMR227C)|FD-Score:-3.25|P-value:5.75E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:YAH1(YPL252C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.19||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:CBF2(YGR140W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.09||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CEF1(YMR213W)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.07||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:GPI11(YDR302W)|FD-Score:-6.7|P-value:1.08E-11|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:IPL1(YPL209C)|FD-Score:4.48|P-value:3.73E-6|Clearance:0.5||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:IRR1(YIL026C)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.08||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NOC2(YOR206W)|FD-Score:3.79|P-value:7.50E-5|Clearance:0.22||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:POL31(YJR006W)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.02||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:PRE1(YER012W)|FD-Score:5.48|P-value:2.16E-8|Clearance:1||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:QRI1(YDL103C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.1||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:3.29|P-value:5.06E-4|Clearance:0.16||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT2(YDL007W)|FD-Score:3.98|P-value:3.47E-5|Clearance:0.11||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:STH1(YIL126W)|FD-Score:-4.09|P-value:2.12E-5|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TAF7(YMR227C)|FD-Score:-3.25|P-value:5.75E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:YAH1(YPL252C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.19||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 64556
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ALE1(YOR175C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:APL5(YPL195W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ATG22(YCL038C)|FD-Score:5.06|P-value:2.07E-7||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:AVO2(YMR068W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BRE1(YDL074C)|FD-Score:-4.28|P-value:9.24E-6||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BUD25(YER014C-A)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:BUD26(YDR241W_d)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBR1(YIL043C)|FD-Score:-3.9|P-value:4.75E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCS1(YMR038C)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLC1(YGR167W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CYM1(YDR430C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Gene:DAL81(YIR023W)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DGK1(YOR311C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DLD2(YDL178W)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EAF5(YEL018W)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:ECM27(YJR106W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:ERG2(YMR202W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERP2(YAL007C)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FMP33(YJL161W_p)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAS1(YMR307W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GIP3(YPL137C)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GLO4(YOR040W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GSY1(YFR015C)|FD-Score:3.76|P-value:8.49E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GYP7(YDL234C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:HSL7(YBR133C)|FD-Score:4.79|P-value:8.36E-7||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HSP12(YFL014W)|FD-Score:4.37|P-value:6.31E-6||SGD DESC:Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension Gene:IAH1(YOR126C)|FD-Score:4.34|P-value:7.06E-6||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:ICT1(YLR099C)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:ITR1(YDR497C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KTR4(YBR199W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LAP2(YNL045W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MCH1(YDL054C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MCH5(YOR306C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MLF3(YNL074C)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide Gene:MRP1(YDR347W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPS35(YGR165W)|FD-Score:7.35|P-value:9.82E-14||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSH4(YFL003C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein Gene:MTC5(YDR128W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:MYO5(YMR109W)|FD-Score:5.1|P-value:1.68E-7||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:NMD2(YHR077C)|FD-Score:3.73|P-value:9.53E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:OCA1(YNL099C)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PCL9(YDL179W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PDC6(YGR087C)|FD-Score:-3.79|P-value:7.47E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PET130(YJL023C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX12(YMR026C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PFS1(YHR185C)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PIB2(YGL023C)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PKP1(YIL042C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p Gene:PMD1(YER132C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:POA1(YBR022W)|FD-Score:4.3|P-value:8.68E-6||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PRM3(YPL192C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body Gene:PRM6(YML047C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:PTC5(YOR090C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:QCR7(YDR529C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RGA2(YDR379W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RPL31B(YLR406C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:4.31|P-value:7.99E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RRT1(YBL048W_d)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RRT2(YBR246W)|FD-Score:-5.83|P-value:2.70E-9||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SBH2(YER019C-A)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SEG2(YKL105C_p)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SFK1(YKL051W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane Gene:SIP4(YJL089W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SKG3(YLR187W_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SKI7(YOR076C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SNF11(YDR073W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SPT3(YDR392W)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SSD1(YDR293C)|FD-Score:-4.7|P-value:1.30E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STL1(YDR536W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:STO1(YMR125W)|FD-Score:-4.85|P-value:6.21E-7||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUM1(YDR310C)|FD-Score:5.37|P-value:3.94E-8||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TAN1(YGL232W)|FD-Score:4.89|P-value:5.05E-7||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THR4(YCR053W)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRE1(YPL176C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TSA2(YDR453C)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress Gene:UBI4(YLL039C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UGA4(YDL210W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane Gene:URC2(YDR520C_p)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VAM10(YOR068C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VPS45(YGL095C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR027C(YBR027C_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR016W(YCR016W_p)|FD-Score:5.04|P-value:2.28E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR133C(YDR133C_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR521W(YDR521W_d)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YER097W(YER097W_d)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL165C(YGL165C_d)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGR127W(YGR127W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR149W(YGR149W_p)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YIG1(YPL201C)|FD-Score:5.29|P-value:5.97E-8||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL166C(YIL166C_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR146W(YJR146W_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YLR001C(YLR001C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR122C(YLR122C_d)|FD-Score:-3.98|P-value:3.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR225C(YLR225C_p)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR236C(YLR236C_d)|FD-Score:4|P-value:3.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR279C(YMR279C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL205C(YNL205C_d)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL019W(YOL019W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOL160W(YOL160W_d)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL164W-A(YOL164W-A_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOP1(YPR028W)|FD-Score:-3.73|P-value:9.52E-5||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR131C(YOR131C_p)|FD-Score:11|P-value:2.28E-28||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR152C(YOR152C_p)|FD-Score:4.91|P-value:4.65E-7||SGD DESC:Putative protein of unknown function; YOR152C is not an essential gene Gene:YOR238W(YOR238W_p)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPK2(YMR104C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPL073C(YPL073C_d)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL245W(YPL245W_p)|FD-Score:-3.81|P-value:6.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR147C(YPR147C_p)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:AAC3(YBR085W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:ALE1(YOR175C)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:APL5(YPL195W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ATG22(YCL038C)|FD-Score:5.06|P-value:2.07E-7||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:AVO2(YMR068W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BRE1(YDL074C)|FD-Score:-4.28|P-value:9.24E-6||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BUD25(YER014C-A)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:BUD26(YDR241W_d)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBR1(YIL043C)|FD-Score:-3.9|P-value:4.75E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCS1(YMR038C)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLC1(YGR167W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CYM1(YDR430C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Gene:DAL81(YIR023W)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DGK1(YOR311C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DLD2(YDL178W)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EAF5(YEL018W)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:ECM27(YJR106W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:ERG2(YMR202W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERP2(YAL007C)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FMP33(YJL161W_p)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAS1(YMR307W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GIP3(YPL137C)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GLO4(YOR040W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GSY1(YFR015C)|FD-Score:3.76|P-value:8.49E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GYP7(YDL234C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking Gene:HSL7(YBR133C)|FD-Score:4.79|P-value:8.36E-7||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HSP12(YFL014W)|FD-Score:4.37|P-value:6.31E-6||SGD DESC:Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension Gene:IAH1(YOR126C)|FD-Score:4.34|P-value:7.06E-6||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:ICT1(YLR099C)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:ITR1(YDR497C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KTR4(YBR199W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LAP2(YNL045W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MCH1(YDL054C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MCH5(YOR306C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MLF3(YNL074C)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide Gene:MRP1(YDR347W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPS35(YGR165W)|FD-Score:7.35|P-value:9.82E-14||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSH4(YFL003C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein Gene:MTC5(YDR128W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:MYO5(YMR109W)|FD-Score:5.1|P-value:1.68E-7||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:NMD2(YHR077C)|FD-Score:3.73|P-value:9.53E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:OCA1(YNL099C)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PCL9(YDL179W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PDC6(YGR087C)|FD-Score:-3.79|P-value:7.47E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PET130(YJL023C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PEX12(YMR026C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PFS1(YHR185C)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PIB2(YGL023C)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PKP1(YIL042C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p Gene:PMD1(YER132C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:POA1(YBR022W)|FD-Score:4.3|P-value:8.68E-6||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PRM3(YPL192C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body Gene:PRM6(YML047C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:PTC5(YOR090C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:QCR7(YDR529C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RGA2(YDR379W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RPL31B(YLR406C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:4.31|P-value:7.99E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RRT1(YBL048W_d)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RRT2(YBR246W)|FD-Score:-5.83|P-value:2.70E-9||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SBH2(YER019C-A)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SEG2(YKL105C_p)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SFK1(YKL051W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane Gene:SIP4(YJL089W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SKG3(YLR187W_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SKI7(YOR076C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SNF11(YDR073W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SPT3(YDR392W)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SSD1(YDR293C)|FD-Score:-4.7|P-value:1.30E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STL1(YDR536W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:STO1(YMR125W)|FD-Score:-4.85|P-value:6.21E-7||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUM1(YDR310C)|FD-Score:5.37|P-value:3.94E-8||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TAN1(YGL232W)|FD-Score:4.89|P-value:5.05E-7||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THR4(YCR053W)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRE1(YPL176C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TSA2(YDR453C)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress Gene:UBI4(YLL039C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UGA4(YDL210W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane Gene:URC2(YDR520C_p)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VAM10(YOR068C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VPS45(YGL095C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR027C(YBR027C_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR016W(YCR016W_p)|FD-Score:5.04|P-value:2.28E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR133C(YDR133C_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR521W(YDR521W_d)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YER097W(YER097W_d)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL165C(YGL165C_d)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGR127W(YGR127W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR149W(YGR149W_p)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YIG1(YPL201C)|FD-Score:5.29|P-value:5.97E-8||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL166C(YIL166C_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR146W(YJR146W_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YLR001C(YLR001C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR122C(YLR122C_d)|FD-Score:-3.98|P-value:3.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR225C(YLR225C_p)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR236C(YLR236C_d)|FD-Score:4|P-value:3.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR279C(YMR279C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL205C(YNL205C_d)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL019W(YOL019W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOL160W(YOL160W_d)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL164W-A(YOL164W-A_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOP1(YPR028W)|FD-Score:-3.73|P-value:9.52E-5||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR131C(YOR131C_p)|FD-Score:11|P-value:2.28E-28||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR152C(YOR152C_p)|FD-Score:4.91|P-value:4.65E-7||SGD DESC:Putative protein of unknown function; YOR152C is not an essential gene Gene:YOR238W(YOR238W_p)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPK2(YMR104C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPL073C(YPL073C_d)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL245W(YPL245W_p)|FD-Score:-3.81|P-value:6.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR147C(YPR147C_p)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER012W5.482.16E-81.00PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YPL209C4.483.73E-60.50IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
YDL007W3.983.47E-50.11RPT2One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
YIL026C3.875.53E-50.08IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YOR206W3.797.50E-50.22NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YGR140W3.571.79E-40.09CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YDL103C3.482.53E-40.10QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YMR213W3.383.64E-40.07CEF1Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
YJR006W3.314.71E-40.02POL31Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair
YHR062C3.295.06E-40.16RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPL252C3.138.70E-40.19YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YOR249C2.940.001620.03APC5Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
YOL021C2.920.001770.05DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YLR147C2.870.002050.03SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YLR127C2.840.002220.01APC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR131C_p11.002.28E-28YOR131C_pPutative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress
YGR165W7.359.82E-14MRPS35Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets
YDR392W5.803.27E-9SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YDR310C5.373.94E-8SUM1Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
YPL201C5.295.97E-8YIG1Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol
YMR109W5.101.68E-7MYO5One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
YDR128W5.081.92E-7MTC5Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress
YCL038C5.062.07E-7ATG22Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation
YCR016W_p5.042.28E-7YCR016W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis
YOR152C_p4.914.65E-7YOR152C_pPutative protein of unknown function; YOR152C is not an essential gene
YGL232W4.895.05E-7TAN1Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
YBR133C4.798.36E-7HSL7Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress
YBR069C4.751.01E-6TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YKL105C_p4.513.28E-6SEG2_pEisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes
YDR521W_d4.493.52E-6YDR521W_dDubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol

GO enrichment analysis for SGTC_2014
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0993.12E-14SGTC_29329016711 44.5 μMChembridge (Drug-like library)71173280.0175439
0.0814.41E-10SGTC_486niguldipine 82.0 μMMiscellaneous12360.0512821amide catabolism
0.0773.25E-9SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)463887NA
0.0708.44E-8SGTC_1614st002383 48.6 μMTimTec (Natural product derivative library)19472350.0149254cell wall signaling
0.0691.09E-7SGTC_31679103034 49.5 μMChembridge (Drug-like library)51278590.0188679RPP1 & pyrimidine depletion
0.0655.67E-7SGTC_31639099914 49.5 μMChembridge (Drug-like library)16268368NA
0.0655.83E-7SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)2843522NAERG2
0.0656.18E-7SGTC_29769009005 19.5 μMChembridge (Drug-like library)29931480.0178571
0.0622.23E-6SGTC_24605552655 174.2 μMMiscellaneous5401989NAamide catabolism
0.0612.56E-6SGTC_12580671-0043 57.1 μMChemDiv (Drug-like library)4298767NA
0.0587.10E-6SGTC_8700573-2213 57.6 μMChemDiv (Drug-like library)3916321NAERG2
0.0587.87E-6SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.02
0.0571.14E-5SGTC_30049076122 71.4 μMChembridge (Drug-like library)447825510.0357143
0.0571.32E-5SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0188679
0.0561.64E-5SGTC_1524469-0003 551.2 μMChemDiv (Drug-like library)6972800.0212766

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_180111-0177500 nM0.275862721752ChemDiv (Drug-like library)336.062844.06901
SGTC_360335-088114.2 μM0.27523828853ChemDiv (Drug-like library)329.477886.29201ubiquinone biosynthesis & proteasome
SGTC_3670335-08817.1 μM0.27523828853ChemDiv (Drug-like library)329.477886.29201ubiquinone biosynthesis & proteasome
SGTC_20555235184200 μM0.2727272836034Chembridge (Fragment library)283.836743.312ERG2
SGTC_6820kpi-009917 μM0.2647061714895ChemDiv (Drug-like library)235.345182.86503
SGTC_9541189-08532.85 μM0.2631582828194ChemDiv (Drug-like library)255.39783.55111endomembrane recycling
SGTC_1050111-0055734.78 μM0.2580655179370ChemDiv (Drug-like library)272.181422.45511
SGTC_450kpi-003614.9 μM0.2571432795021ChemDiv (Drug-like library)242.355984.48311excess fatty acid
SGTC_13081187-161741.4 μM0.2558142882570ChemDiv (Drug-like library)315.449763.31523fatty acid desaturase (OLE1)
SGTC_6420335-0876158 μM0.2553196812350ChemDiv (Drug-like library)424.373345.41712ubiquinone biosynthesis & proteasome
SGTC_12920kpi-0043232 μM0.239133333905ChemDiv (Drug-like library)315.363643.51814