5119600

3-methyl-2,3,4,9-tetrahydropyrido[3,4-b]indol-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2015
Screen concentration 184.0 μM
Source Chembridge (Fragment library)
PubChem CID 2828786
SMILES CC1CC2=C(C(=O)N1)NC3=CC=CC=C23
Standardized SMILES CC1Cc2c([nH]c3ccccc23)C(=O)N1
Molecular weight 200.2365
ALogP 2.08
H-bond donor count 2
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.3
% growth inhibition (Hom. pool) 9.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2828786
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:CCA1(YER168C)|FD-Score:4.41|P-value:5.18E-6|Clearance:0.24||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCT7(YJL111W)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.03||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC11(YJR076C)|FD-Score:3.14|P-value:8.41E-4|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC42(YLR229C)|FD-Score:-5.62|P-value:9.82E-9|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:FAF1(YIL019W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.04||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:ISD11(YER048W-A)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.05||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LSM2(YBL026W)|FD-Score:-3.21|P-value:6.75E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.16||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NOG1(YPL093W)|FD-Score:4.58|P-value:2.38E-6|Clearance:0.17||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NUP49(YGL172W)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.03||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PGA3(YML125C)|FD-Score:3.19|P-value:7.20E-4|Clearance:0.05||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:PIK1(YNL267W)|FD-Score:4.84|P-value:6.47E-7|Clearance:0.13||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRE1(YER012W)|FD-Score:3.22|P-value:6.34E-4|Clearance:0.01||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PZF1(YPR186C)|FD-Score:4.17|P-value:1.52E-5|Clearance:0.31||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RET2(YFR051C)|FD-Score:4.72|P-value:1.21E-6|Clearance:0.14||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RHO1(YPR165W)|FD-Score:-3.91|P-value:4.54E-5|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB3(YIL021W)|FD-Score:-4.87|P-value:5.49E-7|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPS13(YDR064W)|FD-Score:6.76|P-value:6.69E-12|Clearance:1.1||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RRP4(YHR069C)|FD-Score:5.67|P-value:7.25E-9|Clearance:0.83||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC20(YDR498C)|FD-Score:-3.61|P-value:1.56E-4|Clearance:0||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SPT6(YGR116W)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.06||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:STS1(YIR011C)|FD-Score:3.13|P-value:8.67E-4|Clearance:0.03||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:TAF7(YMR227C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.06||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TFB1(YDR311W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TIF34(YMR146C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.2||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:UBC1(YDR177W)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UFE1(YOR075W)|FD-Score:-3.16|P-value:7.82E-4|Clearance:0||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:YOR146W(YOR146W_d)|FD-Score:3.35|P-value:3.99E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ACP1(YKL192C)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:CCA1(YER168C)|FD-Score:4.41|P-value:5.18E-6|Clearance:0.24||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCT7(YJL111W)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.03||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC11(YJR076C)|FD-Score:3.14|P-value:8.41E-4|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC42(YLR229C)|FD-Score:-5.62|P-value:9.82E-9|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:FAF1(YIL019W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.04||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:ISD11(YER048W-A)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.05||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LSM2(YBL026W)|FD-Score:-3.21|P-value:6.75E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.16||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NOG1(YPL093W)|FD-Score:4.58|P-value:2.38E-6|Clearance:0.17||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NUP49(YGL172W)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.03||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PGA3(YML125C)|FD-Score:3.19|P-value:7.20E-4|Clearance:0.05||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:PIK1(YNL267W)|FD-Score:4.84|P-value:6.47E-7|Clearance:0.13||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRE1(YER012W)|FD-Score:3.22|P-value:6.34E-4|Clearance:0.01||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PZF1(YPR186C)|FD-Score:4.17|P-value:1.52E-5|Clearance:0.31||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RET2(YFR051C)|FD-Score:4.72|P-value:1.21E-6|Clearance:0.14||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RHO1(YPR165W)|FD-Score:-3.91|P-value:4.54E-5|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB3(YIL021W)|FD-Score:-4.87|P-value:5.49E-7|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPS13(YDR064W)|FD-Score:6.76|P-value:6.69E-12|Clearance:1.1||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RRP4(YHR069C)|FD-Score:5.67|P-value:7.25E-9|Clearance:0.83||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC20(YDR498C)|FD-Score:-3.61|P-value:1.56E-4|Clearance:0||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SPT6(YGR116W)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.06||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:STS1(YIR011C)|FD-Score:3.13|P-value:8.67E-4|Clearance:0.03||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:TAF7(YMR227C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.06||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TFB1(YDR311W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TIF34(YMR146C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.2||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:UBC1(YDR177W)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UFE1(YOR075W)|FD-Score:-3.16|P-value:7.82E-4|Clearance:0||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:YOR146W(YOR146W_d)|FD-Score:3.35|P-value:3.99E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2828786
Download HOP data (tab-delimited text)  (excel)
Gene:ALF1(YNL148C)|FD-Score:4.29|P-value:8.97E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APL5(YPL195W)|FD-Score:-5.9|P-value:1.76E-9||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APM4(YOL062C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARG4(YHR018C)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ARO1(YDR127W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG11(YPR049C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATX1(YNL259C)|FD-Score:-3.87|P-value:5.54E-5||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BNR1(YIL159W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BRE1(YDL074C)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CIR1(YGR207C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response Gene:DAL3(YIR032C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DSK2(YMR276W)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:EMP24(YGL200C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERG2(YMR202W)|FD-Score:5.61|P-value:1.01E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERP4(YOR016C)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FMS1(YMR020W)|FD-Score:5.24|P-value:8.02E-8||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:GCN3(YKR026C)|FD-Score:11.1|P-value:9.12E-29||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLC8(YMR311C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HLJ1(YMR161W)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HST1(YOL068C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HVG1(YER039C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:ICY2(YPL250C)|FD-Score:8.46|P-value:1.31E-17||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IES3(YLR052W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRS4(YKR019C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MCH1(YDL054C)|FD-Score:-4.88|P-value:5.44E-7||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MKK1(YOR231W)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MND2(YIR025W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNT4(YNR059W)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRPL11(YDL202W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAT3(YPR131C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NCE102(YPR149W)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:NIT2(YJL126W)|FD-Score:4.87|P-value:5.70E-7||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NPP1(YCR026C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NPR1(YNL183C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:ORM1(YGR038W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSH2(YDL019C)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAC1(YOR269W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly Gene:PAM1(YDR251W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PES4(YFR023W)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET127(YOR017W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PEX15(YOL044W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PGM1(YKL127W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PHO4(YFR034C)|FD-Score:7.19|P-value:3.13E-13||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PIB2(YGL023C)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:POT1(YIL160C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:QCR9(YGR183C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RAD23(YEL037C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RFM1(YOR279C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIB4(YOL143C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RMA1(YKL132C)|FD-Score:-4.3|P-value:8.58E-6||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL39(YJL189W)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RTG1(YOL067C)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTK1(YDL025C)|FD-Score:-4.36|P-value:6.43E-6||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEG2(YKL105C_p)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SHB17(YKR043C)|FD-Score:-7.69|P-value:7.32E-15||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHM2(YLR058C)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SNA4(YDL123W)|FD-Score:-3.76|P-value:8.47E-5||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SPT2(YER161C)|FD-Score:9.19|P-value:1.89E-20||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SRO9(YCL037C)|FD-Score:-5.04|P-value:2.35E-7||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSB1(YDL229W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:4.36|P-value:6.60E-6||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TAD1(YGL243W)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala Gene:TGL2(YDR058C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TRK1(YJL129C)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRP1(YDR007W)|FD-Score:4.94|P-value:3.98E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.66|P-value:7.67E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.43|P-value:5.34E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TVP23(YDR084C)|FD-Score:-4.34|P-value:7.22E-6||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBR2(YLR024C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:VMA7(YGR020C)|FD-Score:6.83|P-value:4.37E-12||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS1(YKR001C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS38(YLR360W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:VPS72(YDR485C)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR113W(YBR113W_d)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR137W(YBR137W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene Gene:YCR015C(YCR015C_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDL073W(YDL073W_p)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.86|P-value:3.55E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL242C(YGL242C_p)|FD-Score:-4.41|P-value:5.28E-6||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR126W(YGR126W_p)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR204C-A(YGR204C-A_p)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR237C(YGR237C_p)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIR024C(YIR024C)|FD-Score:-5.37|P-value:3.89E-8||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YLR122C(YLR122C_d)|FD-Score:6.51|P-value:3.67E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR125W(YLR125W_p)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR345W(YLR345W_p)|FD-Score:-3.25|P-value:5.73E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YMR102C(YMR102C_p)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Putative protein of unknown function Gene:YNL266W(YNL266W_d)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YNR063W(YNR063W_p)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR121C(YOR121C_d)|FD-Score:6.04|P-value:7.70E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YOR342C(YOR342C_p)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL247C(YPL247C_p)|FD-Score:-4.46|P-value:4.04E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR003C(YPR003C_p)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:ZRC1(YMR243C)|FD-Score:4.33|P-value:7.46E-6||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:4.29|P-value:8.97E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APL5(YPL195W)|FD-Score:-5.9|P-value:1.76E-9||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APM4(YOL062C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARG4(YHR018C)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ARO1(YDR127W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG11(YPR049C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATX1(YNL259C)|FD-Score:-3.87|P-value:5.54E-5||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BNR1(YIL159W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BRE1(YDL074C)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CIR1(YGR207C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response Gene:DAL3(YIR032C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DSK2(YMR276W)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:EMP24(YGL200C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERG2(YMR202W)|FD-Score:5.61|P-value:1.01E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERP4(YOR016C)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FMS1(YMR020W)|FD-Score:5.24|P-value:8.02E-8||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:GCN3(YKR026C)|FD-Score:11.1|P-value:9.12E-29||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLC8(YMR311C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HLJ1(YMR161W)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HST1(YOL068C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HVG1(YER039C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:ICY2(YPL250C)|FD-Score:8.46|P-value:1.31E-17||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IES3(YLR052W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRS4(YKR019C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MCH1(YDL054C)|FD-Score:-4.88|P-value:5.44E-7||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MKK1(YOR231W)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MND2(YIR025W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNT4(YNR059W)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRPL11(YDL202W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAT3(YPR131C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NCE102(YPR149W)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:NIT2(YJL126W)|FD-Score:4.87|P-value:5.70E-7||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NPP1(YCR026C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NPR1(YNL183C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:ORM1(YGR038W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSH2(YDL019C)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAC1(YOR269W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly Gene:PAM1(YDR251W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PES4(YFR023W)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET127(YOR017W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PEX15(YOL044W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PGM1(YKL127W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PHO4(YFR034C)|FD-Score:7.19|P-value:3.13E-13||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PIB2(YGL023C)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:POT1(YIL160C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:QCR9(YGR183C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RAD23(YEL037C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RFM1(YOR279C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIB4(YOL143C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RMA1(YKL132C)|FD-Score:-4.3|P-value:8.58E-6||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL39(YJL189W)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RTG1(YOL067C)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTK1(YDL025C)|FD-Score:-4.36|P-value:6.43E-6||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEG2(YKL105C_p)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SHB17(YKR043C)|FD-Score:-7.69|P-value:7.32E-15||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHM2(YLR058C)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SNA4(YDL123W)|FD-Score:-3.76|P-value:8.47E-5||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SPT2(YER161C)|FD-Score:9.19|P-value:1.89E-20||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SRO9(YCL037C)|FD-Score:-5.04|P-value:2.35E-7||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSB1(YDL229W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:4.36|P-value:6.60E-6||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TAD1(YGL243W)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala Gene:TGL2(YDR058C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TRK1(YJL129C)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRP1(YDR007W)|FD-Score:4.94|P-value:3.98E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.66|P-value:7.67E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.43|P-value:5.34E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TVP23(YDR084C)|FD-Score:-4.34|P-value:7.22E-6||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBR2(YLR024C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:VMA7(YGR020C)|FD-Score:6.83|P-value:4.37E-12||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS1(YKR001C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS38(YLR360W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:VPS72(YDR485C)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR113W(YBR113W_d)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR137W(YBR137W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene Gene:YCR015C(YCR015C_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDL073W(YDL073W_p)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.86|P-value:3.55E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL242C(YGL242C_p)|FD-Score:-4.41|P-value:5.28E-6||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR126W(YGR126W_p)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR204C-A(YGR204C-A_p)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR237C(YGR237C_p)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIR024C(YIR024C)|FD-Score:-5.37|P-value:3.89E-8||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YLR122C(YLR122C_d)|FD-Score:6.51|P-value:3.67E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR125W(YLR125W_p)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR345W(YLR345W_p)|FD-Score:-3.25|P-value:5.73E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YMR102C(YMR102C_p)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Putative protein of unknown function Gene:YNL266W(YNL266W_d)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YNR063W(YNR063W_p)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR121C(YOR121C_d)|FD-Score:6.04|P-value:7.70E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YOR342C(YOR342C_p)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL247C(YPL247C_p)|FD-Score:-4.46|P-value:4.04E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR003C(YPR003C_p)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:ZRC1(YMR243C)|FD-Score:4.33|P-value:7.46E-6||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YDR064W6.766.69E-121.10RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YHR069C5.677.25E-90.83RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YNL267W4.846.47E-70.12PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YFR051C4.721.21E-60.14RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YPL093W4.582.38E-60.17NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YER168C4.415.18E-60.24CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YPR186C4.171.52E-50.30PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YPL169C3.875.54E-50.16MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YGR116W3.711.05E-40.06SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YMR146C3.651.32E-40.20TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YER048W-A3.442.87E-40.05ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YIL019W3.403.42E-40.04FAF1Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
YOR146W_d3.353.99E-40.10YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YGL172W3.255.73E-40.03NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YER012W3.226.34E-40.01PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YKR026C11.109.12E-29GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YER161C9.191.89E-20SPT2Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins
YPL250C8.461.31E-17ICY2Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate
YDR354W7.435.34E-14TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YFR034C7.193.13E-13PHO4Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability
YDR008C_d6.863.55E-12YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGR020C6.834.37E-12VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YLR122C_d6.513.67E-11YLR122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C
YOR121C_d6.047.70E-10YOR121C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W
YKL211C5.667.67E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YMR202W5.611.01E-8ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YMR020W5.248.02E-8FMS1Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
YHR018C5.081.84E-7ARG4Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway
YDR007W4.943.98E-7TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YJL126W4.875.70E-7NIT2Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member

GO enrichment analysis for SGTC_2015
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2869.27E-112SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.126984
0.2565.19E-89SGTC_24885268135 45.7 μMMiscellaneous12703560.160714
0.2482.29E-83SGTC_20245144227 134.0 μMChembridge (Fragment library)34752110.131148
0.2444.65E-81SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0617284
0.2432.13E-80SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.08
0.2419.40E-79SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.1
0.2362.42E-75SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0923077
0.2346.48E-74SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.126984
0.2312.60E-72SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.133333
0.2291.34E-70SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.0588235TRP & mitochondrial translation
0.2277.81E-70SGTC_18825615643 20.0 μMMiscellaneous22530750.0779221TRP & mitochondrial translation
0.2231.67E-67SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0701754
0.2233.40E-67SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.2202.49E-65SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.078125
0.2151.64E-62SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0921053

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1953st07680012.1 μM0.386364297591TimTec (Natural product derivative library)219.281124.53410
SGTC_1885st05754699.9 μM0.367347702008TimTec (Natural product derivative library)200.236482.18321
SGTC_1972st07020117.4 μM0.3275862829099TimTec (Natural product derivative library)326.77691.10933
SGTC_15998-chlro-1-tetrahydronorharmanone90.6 μM0.3137264174840TimTec (Pure natural product library)220.654962.36221
SGTC_1636st00770768.9 μM0.298246403051TimTec (Natural product derivative library)290.359023.75911
SGTC_1954st07703268.9 μM0.298246698907TimTec (Natural product derivative library)290.359023.68611
SGTC_1516st013856110 μM0.2765965281404TimTec (Pure natural product library)182.22122.45511
SGTC_1993st07399570.8 μM0.2741943098394TimTec (Natural product derivative library)282.423144.94511
SGTC_489paxilline115 μM0.27027105008ICCB bioactive library435.555223.934PDR1
SGTC_2591skatole100 μM0.2619056736Microsource (Natural product library)131.174462.60910