5132927

1-(1H-indol-3-yl)butan-2-amine

Originally believed to exert its effects predominantly via monoamine oxidase inhibition, etryptamine was developed during the 1960s as an antidepressant by Upjohn as Monase but was withdrawn from potential commercial use due to an unacceptable incidence of agranulocytosis

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2017
Screen concentration 60.6 μM
Source Chembridge (Fragment library)
PubChem CID 8367
SMILES CCC(CC1=CNC2=CC=CC=C21)N
Standardized SMILES CCC(N)Cc1c[nH]c2ccccc12
Molecular weight 188.2688
ALogP 2.45
H-bond donor count 2
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.25
% growth inhibition (Hom. pool) 7.76


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 8367
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:3.23|P-value:6.21E-4|Clearance:0.01||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:FRS2(YFL022C)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.24||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GPI8(YDR331W)|FD-Score:5.08|P-value:1.88E-7|Clearance:0.54||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:GPN2(YOR262W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.08||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:IDI1(YPL117C)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.14||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:MCM3(YEL032W)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.03||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NUP82(YJL061W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.07||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PIK1(YNL267W)|FD-Score:3.76|P-value:8.33E-5|Clearance:0.11||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RAD53(YPL153C)|FD-Score:-3.67|P-value:1.23E-4|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RHO1(YPR165W)|FD-Score:-4.81|P-value:7.41E-7|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RRP14(YKL082C)|FD-Score:-3.52|P-value:2.12E-4|Clearance:0||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:SEC23(YPR181C)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.03||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC8(YPR055W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.21||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SUP35(YDR172W)|FD-Score:-4.88|P-value:5.27E-7|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TAO3(YIL129C)|FD-Score:3.97|P-value:3.66E-5|Clearance:0.2||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:YBR190W(YBR190W_d)|FD-Score:4.28|P-value:9.46E-6|Clearance:0.31||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YLR379W(YLR379W_d)|FD-Score:4.54|P-value:2.85E-6|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C Gene:ARC15(YIL062C)|FD-Score:3.23|P-value:6.21E-4|Clearance:0.01||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:FRS2(YFL022C)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.24||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GPI8(YDR331W)|FD-Score:5.08|P-value:1.88E-7|Clearance:0.54||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:GPN2(YOR262W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.08||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:IDI1(YPL117C)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.14||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:MCM3(YEL032W)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.03||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NUP82(YJL061W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.07||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PIK1(YNL267W)|FD-Score:3.76|P-value:8.33E-5|Clearance:0.11||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RAD53(YPL153C)|FD-Score:-3.67|P-value:1.23E-4|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RHO1(YPR165W)|FD-Score:-4.81|P-value:7.41E-7|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RRP14(YKL082C)|FD-Score:-3.52|P-value:2.12E-4|Clearance:0||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:SEC23(YPR181C)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.03||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC8(YPR055W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.21||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SUP35(YDR172W)|FD-Score:-4.88|P-value:5.27E-7|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TAO3(YIL129C)|FD-Score:3.97|P-value:3.66E-5|Clearance:0.2||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:YBR190W(YBR190W_d)|FD-Score:4.28|P-value:9.46E-6|Clearance:0.31||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YLR379W(YLR379W_d)|FD-Score:4.54|P-value:2.85E-6|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 8367
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ARF1(YDL192W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:AUS1(YOR011W)|FD-Score:-4.77|P-value:9.27E-7||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BNA3(YJL060W)|FD-Score:5.18|P-value:1.10E-7||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BNI1(YNL271C)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BNR1(YIL159W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BSC5(YNR069C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CDC73(YLR418C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB5(YPR120C)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COS111(YBR203W)|FD-Score:6.91|P-value:2.45E-12||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL7(YIR031C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAP1(YPL170W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DGK1(YOR311C)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DGR2(YKL121W_p)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:ELP2(YGR200C)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ERF2(YLR246W)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:FMP23(YBR047W_p)|FD-Score:4.87|P-value:5.66E-7||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUI1(YBL042C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FUM1(YPL262W)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GGA1(YDR358W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLK1(YCL040W)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GUT2(YIL155C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HHT2(YNL031C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HOP1(YIL072W)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HOP2(YGL033W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:ICY2(YPL250C)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IMG2(YCR071C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:JIP3(YLR331C_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:JNM1(YMR294W)|FD-Score:7.1|P-value:6.46E-13||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KTR6(YPL053C)|FD-Score:7.08|P-value:7.27E-13||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LSM12(YHR121W)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LYS1(YIR034C)|FD-Score:3.78|P-value:7.93E-5||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAC1(YMR021C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCH1(YDL054C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MEC3(YLR288C)|FD-Score:5.72|P-value:5.42E-9||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MET17(YLR303W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MNT4(YNR059W)|FD-Score:4.9|P-value:4.87E-7||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRPL51(YPR100W)|FD-Score:-4.56|P-value:2.53E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS9(YBR146W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC1(YML128C)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MVB12(YGR206W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NAT1(YDL040C)|FD-Score:-6.21|P-value:2.70E-10||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NFT1(YKR103W)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:OCA5(YHL029C_p)|FD-Score:6.07|P-value:6.30E-10||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OM45(YIL136W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:OST5(YGL226C-A)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAH1(YMR165C)|FD-Score:4.54|P-value:2.87E-6||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PCK1(YKR097W)|FD-Score:4.91|P-value:4.63E-7||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PFA3(YNL326C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PHO81(YGR233C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:POT1(YIL160C)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPT1(YGR123C)|FD-Score:-4.29|P-value:8.80E-6||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:RGA1(YOR127W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPS30A(YLR287C-A)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT102(YGR275W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:4.31|P-value:8.30E-6||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAK1(YER129W)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SBH1(YER087C-B)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SCW4(YGR279C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SEA4(YBL104C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SEC72(YLR292C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHE9(YDR393W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SKI8(YGL213C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SPL2(YHR136C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SUN4(YNL066W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:SYG1(YIL047C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TDA5(YLR426W_p)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TED1(YIL039W)|FD-Score:5.33|P-value:4.98E-8||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:THR4(YCR053W)|FD-Score:-4.54|P-value:2.87E-6||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIF4631(YGR162W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TOR1(YJR066W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:VID27(YNL212W)|FD-Score:4.33|P-value:7.62E-6||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:VPH2(YKL119C)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:YBL055C(YBL055C)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR016W(YBR016W)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR099C(YBR099C_d)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YBR226C(YBR226C_d)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR535C(YDR535C_d)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YGR273C(YGR273C_p)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHC3(YJL059W)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIR007W(YIR007W_p)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR018C(YKR018C_p)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR125W(YLR125W_p)|FD-Score:-7.56|P-value:2.09E-14||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR184W(YLR184W_d)|FD-Score:5.15|P-value:1.29E-7||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YLR456W(YLR456W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YMR114C(YMR114C)|FD-Score:-5.63|P-value:9.17E-9||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR221C(YMR221C_p)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Putative protein of unknown function Gene:YMR279C(YMR279C)|FD-Score:-3.88|P-value:5.22E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNR064C(YNR064C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL106W(YOL106W_d)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL131W(YOL131W_p)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function Gene:YOR041C(YOR041C_d)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR097C(YOR097C_p)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPL080C(YPL080C_d)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR059C(YPR059C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:AAT2(YLR027C)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ARF1(YDL192W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:AUS1(YOR011W)|FD-Score:-4.77|P-value:9.27E-7||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BNA3(YJL060W)|FD-Score:5.18|P-value:1.10E-7||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BNI1(YNL271C)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BNR1(YIL159W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BSC5(YNR069C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CDC73(YLR418C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLB5(YPR120C)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COS111(YBR203W)|FD-Score:6.91|P-value:2.45E-12||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL7(YIR031C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAP1(YPL170W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DGK1(YOR311C)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DGR2(YKL121W_p)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:ELP2(YGR200C)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ERF2(YLR246W)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:FMP23(YBR047W_p)|FD-Score:4.87|P-value:5.66E-7||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUI1(YBL042C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FUM1(YPL262W)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GGA1(YDR358W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLK1(YCL040W)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GUT2(YIL155C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HHT2(YNL031C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HOP1(YIL072W)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HOP2(YGL033W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:ICY2(YPL250C)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IMG2(YCR071C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:JIP3(YLR331C_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:JNM1(YMR294W)|FD-Score:7.1|P-value:6.46E-13||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KTR6(YPL053C)|FD-Score:7.08|P-value:7.27E-13||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LSM12(YHR121W)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LYS1(YIR034C)|FD-Score:3.78|P-value:7.93E-5||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAC1(YMR021C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCH1(YDL054C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MEC3(YLR288C)|FD-Score:5.72|P-value:5.42E-9||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MET17(YLR303W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MNT4(YNR059W)|FD-Score:4.9|P-value:4.87E-7||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRPL51(YPR100W)|FD-Score:-4.56|P-value:2.53E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS9(YBR146W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC1(YML128C)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MVB12(YGR206W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NAT1(YDL040C)|FD-Score:-6.21|P-value:2.70E-10||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NFT1(YKR103W)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:OCA5(YHL029C_p)|FD-Score:6.07|P-value:6.30E-10||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OM45(YIL136W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:OST5(YGL226C-A)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PAH1(YMR165C)|FD-Score:4.54|P-value:2.87E-6||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PCK1(YKR097W)|FD-Score:4.91|P-value:4.63E-7||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PFA3(YNL326C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PHO81(YGR233C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:POT1(YIL160C)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPT1(YGR123C)|FD-Score:-4.29|P-value:8.80E-6||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:RGA1(YOR127W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPS30A(YLR287C-A)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT102(YGR275W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:4.31|P-value:8.30E-6||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAK1(YER129W)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SBH1(YER087C-B)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SCW4(YGR279C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SEA4(YBL104C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SEC72(YLR292C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHE9(YDR393W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SKI8(YGL213C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SPL2(YHR136C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SUN4(YNL066W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:SYG1(YIL047C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TDA5(YLR426W_p)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TED1(YIL039W)|FD-Score:5.33|P-value:4.98E-8||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:THR4(YCR053W)|FD-Score:-4.54|P-value:2.87E-6||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIF4631(YGR162W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TOR1(YJR066W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:VID27(YNL212W)|FD-Score:4.33|P-value:7.62E-6||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:VPH2(YKL119C)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:YBL055C(YBL055C)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR016W(YBR016W)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR099C(YBR099C_d)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YBR226C(YBR226C_d)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR535C(YDR535C_d)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YGR273C(YGR273C_p)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHC3(YJL059W)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIR007W(YIR007W_p)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR018C(YKR018C_p)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR125W(YLR125W_p)|FD-Score:-7.56|P-value:2.09E-14||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR184W(YLR184W_d)|FD-Score:5.15|P-value:1.29E-7||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YLR456W(YLR456W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YMR114C(YMR114C)|FD-Score:-5.63|P-value:9.17E-9||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR221C(YMR221C_p)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Putative protein of unknown function Gene:YMR279C(YMR279C)|FD-Score:-3.88|P-value:5.22E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNR064C(YNR064C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL106W(YOL106W_d)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL131W(YOL131W_p)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function Gene:YOR041C(YOR041C_d)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR097C(YOR097C_p)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPL080C(YPL080C_d)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR059C(YPR059C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR331W5.081.88E-70.54GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YLR379W_d4.542.85E-60.02YLR379W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C
YFL022C4.523.16E-60.24FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YBR190W_d4.289.46E-60.31YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YIL129C3.973.66E-50.20TAO3Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
YNL267W3.768.33E-50.11PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YEL032W3.651.29E-40.03MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YOR262W3.621.45E-40.08GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YJL061W3.542.00E-40.07NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YPR055W3.472.59E-40.21SEC8Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
YPR181C3.265.49E-40.03SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YIL062C3.236.21E-40.01ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YPL117C3.226.39E-40.14IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
YDL120W3.080.001020.09YFH1Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
YDR302W2.990.001380.03GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR294W7.106.46E-13JNM1Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
YPL053C7.087.27E-13KTR6Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication
YBR203W6.912.45E-12COS111Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YHL029C_p6.076.30E-10OCA5_pCytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
YKR103W5.891.98E-9NFT1Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds.
YLR288C5.725.42E-9MEC3DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
YIL039W5.334.98E-8TED1Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YJL060W5.181.10E-7BNA3Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
YLR184W_d5.151.29E-7YLR184W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR273C_p5.072.02E-7YGR273C_pPutative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene
YKR097W4.914.63E-7PCK1Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
YNR059W4.904.87E-7MNT4Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation
YBR047W_p4.875.66E-7FMP23_pPutative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBR146W4.741.06E-6MRPS9Mitochondrial ribosomal protein of the small subunit
YCR071C4.641.71E-6IMG2Mitochondrial ribosomal protein of the large subunit

GO enrichment analysis for SGTC_2017
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0649.08E-7SGTC_22226885208 172.1 μMChembridge (Fragment library)6095140.109091
0.0621.80E-6SGTC_14863970-0793 4.5 μMChemDiv (Drug-like library)28443300.0545455
0.0588.02E-6SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.0393701
0.0589.16E-6SGTC_9861493-0483 84.0 μMChemDiv (Drug-like library)400790.103448
0.0571.06E-5SGTC_1470k295-0292 231.0 μMChemDiv (Drug-like library)50456120.084507
0.0571.21E-5SGTC_1647st011722 22.4 μMTimTec (Natural product derivative library)28547830.0983607
0.0518.02E-5SGTC_1657st012939 43.4 μMTimTec (Natural product derivative library)242072570.0192308
0.0518.48E-5SGTC_25354',4'-dimethoxydalbergione 51.2 μMMicrosource (Natural product library)3641060.0833333DNA damage response
0.0519.76E-5SGTC_2667digitoxin 100.0 μMMicrosource (Natural product library)57020410.0309278
0.0501.08E-4SGTC_1468k284-1086 134.0 μMChemDiv (Drug-like library)159964570.104478DNA intercalators
0.0501.24E-4SGTC_2649anisomycin 9.6 μMICCB bioactive library66103090.116667
0.0501.31E-4SGTC_2517totarol 10.0 μMMicrosource (Natural product library)4601780.078125
0.0472.75E-4SGTC_10004048-0884 8.7 μMChemDiv (Drug-like library)130400880.0769231copper-dependent oxidative stress
0.0472.88E-4SGTC_13893405-0187 19.0 μMChemDiv (Drug-like library)7998900.101695
0.0473.45E-4SGTC_6841140-0066 328.0 μMChemDiv (Drug-like library)68135340.0853659Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_452diindolylmethane8 μM0.5454553071ICCB bioactive library246.306464.39120RNA processing & uracil transport
SGTC_2588indole-3-carbinol100 μM0.5142863712Microsource (Natural product library)147.173861.51821
SGTC_1826st05565541.6 μM0.45098676168TimTec (Natural product derivative library)329.395063.2332ERG2
SGTC_20515263410176 μM0.44186227584Chembridge (Fragment library)229.320762.15412
SGTC_2591skatole100 μM0.4285716736Microsource (Natural product library)131.174462.60910
SGTC_1931st07443857.1 μM0.4181822787313TimTec (Natural product derivative library)350.410983.91323
SGTC_1825st05214362.6 μM0.404255674173TimTec (Natural product derivative library)319.44334.22412
SGTC_1774st04837148.47 μM0.3958334242727TimTec (Natural product derivative library)318.415483.47412RNA processing & uracil transport
SGTC_1998st07579776.8 μM0.39215724208315TimTec (Natural product derivative library)260.33153.40722mitochondrial response to ROS
SGTC_1754st04206516.14 μM0.3913041126236TimTec (Natural product derivative library)355.47544.64612NEO1-PIK1