3594-0513

6-bromo-2-hydroxybenzo[de]isoquinoline-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_202
Screen concentration 34.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 708429
SMILES C1=CC2=C(C=CC3=C2C(=C1)C(=O)N(C3=O)O)Br
Standardized SMILES ON1C(=O)c2cccc3c(Br)ccc(C1=O)c23
Molecular weight 292.0849
ALogP 2.33
H-bond donor count 1
H-bond acceptor count 3
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.02
% growth inhibition (Hom. pool) 11.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 708429
Download HIP data (tab-delimited text)  (excel)
Gene:DAM1(YGR113W)|FD-Score:-3.13|P-value:8.62E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:ERG12(YMR208W)|FD-Score:3.83|P-value:6.33E-5|Clearance:0.24||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:3.18|P-value:7.32E-4|Clearance:0.05||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NDC1(YML031W)|FD-Score:3.1|P-value:9.58E-4|Clearance:0.02||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:POP4(YBR257W)|FD-Score:6.94|P-value:1.94E-12|Clearance:1.6||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP19(YLL036C)|FD-Score:5.43|P-value:2.85E-8|Clearance:1.6||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RPP0(YLR340W)|FD-Score:-4.63|P-value:1.86E-6|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:7.88|P-value:1.69E-15|Clearance:1.6||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMD2(YLR275W)|FD-Score:7.84|P-value:2.30E-15|Clearance:1.6||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SMP3(YOR149C)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.03||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SPT15(YER148W)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.03||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TID3(YIL144W)|FD-Score:3.59|P-value:1.62E-4|Clearance:0.16||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YLR198C(YLR198C_d)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:DAM1(YGR113W)|FD-Score:-3.13|P-value:8.62E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:ERG12(YMR208W)|FD-Score:3.83|P-value:6.33E-5|Clearance:0.24||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:3.18|P-value:7.32E-4|Clearance:0.05||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NDC1(YML031W)|FD-Score:3.1|P-value:9.58E-4|Clearance:0.02||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:POP4(YBR257W)|FD-Score:6.94|P-value:1.94E-12|Clearance:1.6||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP19(YLL036C)|FD-Score:5.43|P-value:2.85E-8|Clearance:1.6||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RPP0(YLR340W)|FD-Score:-4.63|P-value:1.86E-6|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:7.88|P-value:1.69E-15|Clearance:1.6||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMD2(YLR275W)|FD-Score:7.84|P-value:2.30E-15|Clearance:1.6||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SMP3(YOR149C)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.03||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SPT15(YER148W)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.03||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TID3(YIL144W)|FD-Score:3.59|P-value:1.62E-4|Clearance:0.16||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YLR198C(YLR198C_d)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 708429
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ALD6(YPL061W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:BUD31(YCR063W)|FD-Score:4.27|P-value:9.99E-6||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:COG7(YGL005C)|FD-Score:3.76|P-value:8.45E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUR1(YPR158W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DID2(YKR035W-A)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:GET2(YER083C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GUP1(YGL084C)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HSP78(YDR258C)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:ISY1(YJR050W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:MAP1(YLR244C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MTO1(YGL236C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:NPY1(YGL067W)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member Gene:PDR1(YGL013C)|FD-Score:9.47|P-value:1.43E-21||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:RPA34(YJL148W)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPA49(YNL248C)|FD-Score:4.3|P-value:8.35E-6||SGD DESC:RNA polymerase I subunit A49 Gene:RPN4(YDL020C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RVS161(YCR009C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC6(YDR129C)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCS7(YMR272C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SNC2(YOR327C)|FD-Score:5.54|P-value:1.50E-8||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SRN2(YLR119W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SRO9(YCL037C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:STD1(YOR047C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TOP1(YOL006C)|FD-Score:6.52|P-value:3.48E-11||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRP3(YKL211C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP13(YBL067C)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:ULA1(YPL003W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:URA7(YBL039C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:YMR253C(YMR253C_p)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YMR315W(YMR315W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YPL062W(YPL062W_d)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR053C(YPR053C_d)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:ABZ2(YMR289W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ALD6(YPL061W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:BUD31(YCR063W)|FD-Score:4.27|P-value:9.99E-6||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:COG7(YGL005C)|FD-Score:3.76|P-value:8.45E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUR1(YPR158W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DID2(YKR035W-A)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:GET2(YER083C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GUP1(YGL084C)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HSP78(YDR258C)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:ISY1(YJR050W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:MAP1(YLR244C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MTO1(YGL236C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:NPY1(YGL067W)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member Gene:PDR1(YGL013C)|FD-Score:9.47|P-value:1.43E-21||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:RPA34(YJL148W)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPA49(YNL248C)|FD-Score:4.3|P-value:8.35E-6||SGD DESC:RNA polymerase I subunit A49 Gene:RPN4(YDL020C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RVS161(YCR009C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC6(YDR129C)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCS7(YMR272C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SNC2(YOR327C)|FD-Score:5.54|P-value:1.50E-8||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SRN2(YLR119W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SRO9(YCL037C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:STD1(YOR047C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TOP1(YOL006C)|FD-Score:6.52|P-value:3.48E-11||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRP3(YKL211C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP13(YBL067C)|FD-Score:-4.23|P-value:1.19E-5||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:ULA1(YPL003W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:URA7(YBL039C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:YMR253C(YMR253C_p)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YMR315W(YMR315W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YPL062W(YPL062W_d)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR053C(YPR053C_d)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C7.881.69E-151.60RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR275W7.842.30E-151.60SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YBR257W6.941.94E-121.60POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YLL036C5.432.85E-81.60PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YMR208W3.836.33E-50.24ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YIL144W3.591.62E-40.16TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YLR198C_d3.432.97E-40.22YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YOR149C3.226.51E-40.03SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YJL085W3.187.32E-40.05EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YER148W3.138.72E-40.03SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YML031W3.109.58E-40.02NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YGR156W3.080.001030.03PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YDL150W3.050.001140.01RPC53RNA polymerase III subunit C53
YNL126W3.040.001190.04SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YMR268C3.000.001370.04PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C9.471.43E-21PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YOL006C6.523.48E-11TOP1Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination
YJL148W6.232.28E-10RPA34RNA polymerase I subunit A34.5
YOR327C5.541.50E-8SNC2Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication
YPR158W4.741.06E-6CUR1Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication
YNL248C4.308.35E-6RPA49RNA polymerase I subunit A49
YCR063W4.279.99E-6BUD31Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
YBL039C4.102.06E-5URA7Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication
YMR253C_p3.924.45E-5YMR253C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
YGL084C3.836.31E-5GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YGL005C3.768.45E-5COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YCL037C3.729.92E-5SRO9Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
YPL061W3.681.18E-4ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YPL003W3.651.31E-4ULA1Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation
YGL236C3.641.38E-4MTO1Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants

GO enrichment analysis for SGTC_202
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2386.53E-77SGTC_389tpck 16.7 μMMiscellaneous4396470.0806452
0.2132.44E-61SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0649351PDR1
0.1944.29E-51SGTC_21565649887 115.0 μMChembridge (Fragment library)54680440NARPP1 & pyrimidine depletion
0.1903.60E-49SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0816327PDR1
0.1906.05E-49SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.128205PDR1
0.1842.93E-46SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0493827PDR1
0.1825.03E-45SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.203704PDR1
0.1779.05E-43SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.135593PDR1
0.1742.44E-41SGTC_31699102970 49.5 μMChembridge (Drug-like library)170278390.103448RPP1 & pyrimidine depletion
0.1711.01E-39SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.078125PDR1
0.1646.62E-37SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.209302copper-dependent oxidative stress
0.1521.16E-31SGTC_6671498-1044 59.0 μMChemDiv (Drug-like library)67399820.140351RPP1 & pyrimidine depletion
0.1475.85E-30SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.15625RPP1 & pyrimidine depletion
0.1462.49E-29SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.117647
0.1454.20E-29SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.117647

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3393091-483371.4 μM0.3061223240077ChemDiv (Drug-like library)272.686463.1713iron homeostasis
SGTC_13853091-483414.1 μM0.3061224072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion
SGTC_1644st01013420.9 μM0.3061223818134TimTec (Natural product derivative library)280.274782.7814mitochondrial processes
SGTC_1683st010134710 nM0.3061223818134TimTec (Natural product derivative library)280.274782.7814
SGTC_14790549-0158134 μM0.35440202ChemDiv (Drug-like library)356.17023.19913
SGTC_1110083-003952.46 μM0.285714759313ChemDiv (Drug-like library)238.281163.55902
SGTC_2671danthron100 μM0.2631582950Microsource (Natural product library)240.210922.32424DNA damage response
SGTC_23945221648200 μM0.26087788586Miscellaneous346.132322.90504
SGTC_544k015-002052.4 μM0.2553193008304ChemDiv (Drug-like library)316.14943.20802
SGTC_12460422-0085105 μM0.243902264295ChemDiv (Drug-like library)161.157381.26512
SGTC_263alizarin107.93 μM0.2380956293Miscellaneous240.210922.32424iron homeostasis