4017018

4-phenylpiperazine-1-carboximidamide; sulfuric acid; hydrate

Used for adrenal and myocardial imaging when labeled with iodine radioisotope.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2020
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 21127491
SMILES C1CN(CCN1C2=CC=CC=C2)C(=N)N.O.OS(=O)(=O)O
Standardized SMILES NC(=N)N1CCN(CC1)c2ccccc2
Molecular weight 320.3653
ALogP 0.24
H-bond donor count 5
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.29
% growth inhibition (Hom. pool) 2.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 21127491
Download HIP data (tab-delimited text)  (excel)
Gene:BET3(YKR068C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.08||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:BPL1(YDL141W)|FD-Score:3.94|P-value:4.04E-5|Clearance:0.25||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:CDC9(YDL164C)|FD-Score:3.18|P-value:7.34E-4|Clearance:0.24||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:DCP2(YNL118C)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.09||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LCB2(YDR062W)|FD-Score:5.59|P-value:1.14E-8|Clearance:0.49||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NMD3(YHR170W)|FD-Score:-4.75|P-value:1.01E-6|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PIK1(YNL267W)|FD-Score:5.09|P-value:1.75E-7|Clearance:0.32||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRP45(YAL032C)|FD-Score:4.77|P-value:9.10E-7|Clearance:0.63||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RET2(YFR051C)|FD-Score:-3.31|P-value:4.65E-4|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:SAR1(YPL218W)|FD-Score:-3.16|P-value:8.00E-4|Clearance:0||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SEC11(YIR022W)|FD-Score:9.39|P-value:2.86E-21|Clearance:2.06||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SEC53(YFL045C)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.11||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SEN54(YPL083C)|FD-Score:4.05|P-value:2.55E-5|Clearance:0.11||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:TFB3(YDR460W)|FD-Score:-3.24|P-value:5.97E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TID3(YIL144W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.19||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TOM40(YMR203W)|FD-Score:3.25|P-value:5.84E-4|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:VTI1(YMR197C)|FD-Score:7.34|P-value:1.10E-13|Clearance:1.75||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YJR012C(YJR012C_p)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.05||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:BET3(YKR068C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.08||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:BPL1(YDL141W)|FD-Score:3.94|P-value:4.04E-5|Clearance:0.25||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:CDC9(YDL164C)|FD-Score:3.18|P-value:7.34E-4|Clearance:0.24||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:DCP2(YNL118C)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.09||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LCB2(YDR062W)|FD-Score:5.59|P-value:1.14E-8|Clearance:0.49||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NMD3(YHR170W)|FD-Score:-4.75|P-value:1.01E-6|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PIK1(YNL267W)|FD-Score:5.09|P-value:1.75E-7|Clearance:0.32||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRP45(YAL032C)|FD-Score:4.77|P-value:9.10E-7|Clearance:0.63||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RET2(YFR051C)|FD-Score:-3.31|P-value:4.65E-4|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:SAR1(YPL218W)|FD-Score:-3.16|P-value:8.00E-4|Clearance:0||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SEC11(YIR022W)|FD-Score:9.39|P-value:2.86E-21|Clearance:2.06||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SEC53(YFL045C)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.11||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SEN54(YPL083C)|FD-Score:4.05|P-value:2.55E-5|Clearance:0.11||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:TFB3(YDR460W)|FD-Score:-3.24|P-value:5.97E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TID3(YIL144W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.19||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TOM40(YMR203W)|FD-Score:3.25|P-value:5.84E-4|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:VTI1(YMR197C)|FD-Score:7.34|P-value:1.10E-13|Clearance:1.75||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YJR012C(YJR012C_p)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.05||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 21127491
Download HOP data (tab-delimited text)  (excel)
Gene:APL2(YKL135C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:APL5(YPL195W)|FD-Score:-5.53|P-value:1.63E-8||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:AVT1(YJR001W)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AVT7(YIL088C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCH1(YMR237W)|FD-Score:-3.89|P-value:5.10E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:CBT1(YKL208W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CIN8(YEL061C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CSG2(YBR036C)|FD-Score:4.61|P-value:1.99E-6||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSM4(YPL200W)|FD-Score:-3.75|P-value:8.85E-5||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CTI6(YPL181W)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DRS2(YAL026C)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSF2(YBR007C_p)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DYN3(YMR299C)|FD-Score:5.45|P-value:2.57E-8||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FAB1(YFR019W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:GGA1(YDR358W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLC8(YMR311C)|FD-Score:6|P-value:9.79E-10||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HOP2(YGL033W)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:INP2(YMR163C)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:KRE6(YPR159W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:MAD2(YJL030W)|FD-Score:4.77|P-value:9.22E-7||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MDM32(YOR147W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MIP6(YHR015W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MOS1(YCL057C-A)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MTC4(YBR255W)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NAT5(YOR253W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NCA3(YJL116C)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:NGG1(YDR176W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NIT1(YIL164C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NUC1(YJL208C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:NUR1(YDL089W)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OLA1(YBR025C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:OXR1(YPL196W)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:OYE2(YHR179W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PEX27(YOR193W)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PRO1(YDR300C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PRS2(YER099C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PTC5(YOR090C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RAS2(YNL098C)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RNP1(YLL046C)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL11B(YGR085C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPP2A(YOL039W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRD2(YPL152W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRT2(YBR246W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SCP1(YOR367W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SUL2(YLR092W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWP82(YFL049W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:SWS2(YNL081C)|FD-Score:5.38|P-value:3.76E-8||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:VAC8(YEL013W)|FD-Score:4.36|P-value:6.40E-6||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VID24(YBR105C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA3(YEL027W)|FD-Score:-4.72|P-value:1.20E-6||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS3(YDR495C)|FD-Score:4.43|P-value:4.68E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS72(YDR485C)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCF1(YDR135C)|FD-Score:-4.62|P-value:1.90E-6||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDR061W(YDR061W_p)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YER091C-A(YER091C-A_d)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGK3(YOL128C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL260W(YGL260W_p)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR126W(YGR126W_p)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YHR180W(YHR180W_d)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL012W(YIL012W_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL168W(YIL168W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJL070C(YJL070C_p)|FD-Score:-5.08|P-value:1.86E-7||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YKL115C(YKL115C_d)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLR125W(YLR125W_p)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR345W(YLR345W_p)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YMR084W(YMR084W_p)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL143C(YNL143C_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YPR039W(YPR039W_d)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR092W(YPR092W_d)|FD-Score:4.85|P-value:6.28E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR147C(YPR147C_p)|FD-Score:-5.1|P-value:1.67E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YPR170C(YPR170C_d)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YSP1(YHR155W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:APL2(YKL135C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:APL5(YPL195W)|FD-Score:-5.53|P-value:1.63E-8||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:AVT1(YJR001W)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AVT7(YIL088C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCH1(YMR237W)|FD-Score:-3.89|P-value:5.10E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:CBT1(YKL208W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CIN8(YEL061C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CSG2(YBR036C)|FD-Score:4.61|P-value:1.99E-6||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSM4(YPL200W)|FD-Score:-3.75|P-value:8.85E-5||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CTI6(YPL181W)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DRS2(YAL026C)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSF2(YBR007C_p)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DYN3(YMR299C)|FD-Score:5.45|P-value:2.57E-8||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FAB1(YFR019W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:GGA1(YDR358W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLC8(YMR311C)|FD-Score:6|P-value:9.79E-10||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HOP2(YGL033W)|FD-Score:3.8|P-value:7.26E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:INP2(YMR163C)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:KRE6(YPR159W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:MAD2(YJL030W)|FD-Score:4.77|P-value:9.22E-7||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MDM32(YOR147W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MIP6(YHR015W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MOS1(YCL057C-A)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MTC4(YBR255W)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NAT5(YOR253W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NCA3(YJL116C)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:NGG1(YDR176W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NIT1(YIL164C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NUC1(YJL208C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:NUR1(YDL089W)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OLA1(YBR025C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:OXR1(YPL196W)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:OYE2(YHR179W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PEX27(YOR193W)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PRO1(YDR300C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PRS2(YER099C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PTC5(YOR090C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RAS2(YNL098C)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RNP1(YLL046C)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL11B(YGR085C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPP2A(YOL039W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRD2(YPL152W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRT2(YBR246W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SCP1(YOR367W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SUL2(YLR092W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWP82(YFL049W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions Gene:SWS2(YNL081C)|FD-Score:5.38|P-value:3.76E-8||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:VAC8(YEL013W)|FD-Score:4.36|P-value:6.40E-6||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VID24(YBR105C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA3(YEL027W)|FD-Score:-4.72|P-value:1.20E-6||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS3(YDR495C)|FD-Score:4.43|P-value:4.68E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS72(YDR485C)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCF1(YDR135C)|FD-Score:-4.62|P-value:1.90E-6||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDR061W(YDR061W_p)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YER091C-A(YER091C-A_d)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGK3(YOL128C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL260W(YGL260W_p)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR126W(YGR126W_p)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YHR180W(YHR180W_d)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL012W(YIL012W_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL168W(YIL168W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJL070C(YJL070C_p)|FD-Score:-5.08|P-value:1.86E-7||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YKL115C(YKL115C_d)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLR125W(YLR125W_p)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR345W(YLR345W_p)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YMR084W(YMR084W_p)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL143C(YNL143C_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YPR039W(YPR039W_d)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR092W(YPR092W_d)|FD-Score:4.85|P-value:6.28E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR147C(YPR147C_p)|FD-Score:-5.1|P-value:1.67E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YPR170C(YPR170C_d)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YSP1(YHR155W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIR022W9.392.86E-212.06SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YMR197C7.341.10E-131.75VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YDR062W5.591.14E-80.49LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YNL267W5.091.75E-70.32PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YAL032C4.779.10E-70.63PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YNL118C4.141.75E-50.09DCP2Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
YPL083C4.052.55E-50.11SEN54Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YDL141W3.944.04E-50.25BPL1Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
YER133W3.691.12E-40.01GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YIL144W3.681.17E-40.19TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YKR068C3.492.40E-40.08BET3Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
YFL045C3.413.27E-40.11SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YJR012C_p3.304.86E-40.05YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YMR203W3.255.84E-40.07TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YDL164C3.187.34E-40.24CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR311C6.009.79E-10GLC8Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
YMR299C5.452.57E-8DYN3Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
YNL081C5.383.76E-8SWS2Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency
YPR039W_d5.141.36E-7YPR039W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W
YPR092W_d4.856.28E-7YPR092W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJL030W4.779.22E-7MAD2Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I
YBR246W4.711.27E-6RRT2Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
YBR036C4.611.99E-6CSG2Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
YPR170C_d4.454.25E-6YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B
YDR495C4.434.68E-6VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YEL013W4.366.40E-6VAC8Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions
YMR084W_p4.231.19E-5YMR084W_pPutative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
YNL143C_p4.161.62E-5YNL143C_pProtein of unknown function; expressed at both mRNA and protein levels
YGL147C4.151.69E-5RPL9ARibosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6
YOL039W4.141.74E-5RPP2ARibosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm

GO enrichment analysis for SGTC_2020
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0672.54E-7SGTC_1803st050068 52.4 μMTimTec (Natural product derivative library)39840590.0615385NEO1-PIK1
0.0641.02E-6SGTC_6800417-1641 114.0 μMChemDiv (Drug-like library)28359900.135593RPP1 & pyrimidine depletion
0.0621.64E-6SGTC_1801st050089 28.4 μMTimTec (Natural product derivative library)50084930.111111NEO1-PIK1
0.0604.32E-6SGTC_2666tannic acid 100.0 μMMicrosource (Natural product library)161342670.0441176iron homeostasis
0.0596.80E-6SGTC_1625st003711 30.1 μMTimTec (Natural product derivative library)242070340.0886076calcium & mitochondrial duress
0.0571.29E-5SGTC_267212a-hydroxy-5-deoxydehydromunduserone 100.0 μMMicrosource (Natural product library)46208980.0625
0.0561.48E-5SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0428571RPP1 & pyrimidine depletion
0.0561.61E-5SGTC_2646retinol 96.8 μMMicrosource (Natural product library)4453540.0483871
0.0552.49E-5SGTC_439amiodarone 34.4 μMMiscellaneous21570.0821918NEO1-PIK1
0.0534.91E-5SGTC_6710560-0003 407.0 μMChemDiv (Drug-like library)32386780.109091
0.0535.24E-5SGTC_22206782180 200.0 μMChembridge (Fragment library)8338030.113208RNA processing & uracil transport
0.0525.68E-5SGTC_20155119600 184.0 μMChembridge (Fragment library)28287860.115385
0.0525.83E-5SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0357143DNA intercalators
0.0501.26E-4SGTC_7441594-0068 283.0 μMChemDiv (Drug-like library)6683920.0888889amide catabolism
0.0491.52E-4SGTC_22567954252 200.0 μMChembridge (Fragment library)29729200.0961538mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2147554593289.72 μM0.514286797404Chembridge (Fragment library)232.32142.33802ergosterol biosynthesis
SGTC_3128912769149.47 μM0.38775541316314Chembridge (Drug-like library)338.426722.32815
SGTC_20505263056171 μM0.384615763468Chembridge (Fragment library)268.35353.29113fatty acid desaturase (OLE1)
SGTC_23849036245200 μM0.3488376462766Chembridge (Fragment library)261.362621.83713
SGTC_23829035899200 μM0.3478266462679Chembridge (Fragment library)288.368041.9431460S ribosome export
SGTC_2155557042376 μM0.341463797402Chembridge (Fragment library)250.3118632.54403ergosterol biosynthesis
SGTC_1779st03942755 μM0.3333331124498TimTec (Natural product derivative library)363.45283.50103
SGTC_21856113692200 μM0.325581874150Chembridge (Fragment library)250.3118632.53703ergosterol biosynthesis
SGTC_1460k062-00599.15 μM0.281373085ChemDiv (Drug-like library)368.2328434.31513Golgi
SGTC_22016605497169.86 μM0.27907670741Chembridge (Fragment library)232.32142.18102ergosterol biosynthesis