5175110

8-hydroxyquinoline-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2030
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 2831986
SMILES C1=CC2=C(C(=C1)O)N=C(C=C2)C(=O)N
Standardized SMILES NC(=O)c1ccc2cccc(O)c2n1
Molecular weight 188.1827
ALogP 1.21
H-bond donor count 2
H-bond acceptor count 3
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.4
% growth inhibition (Hom. pool) 3.66


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2831986
Download HIP data (tab-delimited text)  (excel)
Gene:AUR1(YKL004W)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance Gene:CCT3(YJL014W)|FD-Score:4.38|P-value:6.01E-6|Clearance:0.58||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:MCM2(YBL023C)|FD-Score:3.12|P-value:9.17E-4|Clearance:0.06||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MPS3(YJL019W)|FD-Score:3.25|P-value:5.87E-4|Clearance:0.08||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:MTR2(YKL186C)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.02||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:MTR3(YGR158C)|FD-Score:3.16|P-value:7.76E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NCP1(YHR042W)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.13||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:PRE10(YOR362C)|FD-Score:4.23|P-value:1.14E-5|Clearance:0.58||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:TAF7(YMR227C)|FD-Score:3.98|P-value:3.45E-5|Clearance:0.58||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TRE2(YOR256C)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.58||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:AUR1(YKL004W)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance Gene:CCT3(YJL014W)|FD-Score:4.38|P-value:6.01E-6|Clearance:0.58||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:MCM2(YBL023C)|FD-Score:3.12|P-value:9.17E-4|Clearance:0.06||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MPS3(YJL019W)|FD-Score:3.25|P-value:5.87E-4|Clearance:0.08||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:MTR2(YKL186C)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.02||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:MTR3(YGR158C)|FD-Score:3.16|P-value:7.76E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NCP1(YHR042W)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.13||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:PRE10(YOR362C)|FD-Score:4.23|P-value:1.14E-5|Clearance:0.58||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:TAF7(YMR227C)|FD-Score:3.98|P-value:3.45E-5|Clearance:0.58||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TRE2(YOR256C)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.58||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2831986
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:-4.61|P-value:2.06E-6||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AGP3(YFL055W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:AIF1(YNR074C)|FD-Score:5.77|P-value:3.88E-9||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:APL3(YBL037W)|FD-Score:9.33|P-value:5.12E-21||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:APL5(YPL195W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APM3(YBR288C)|FD-Score:5.69|P-value:6.24E-9||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:AZR1(YGR224W)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BNR1(YIL159W)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BTS1(YPL069C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF40(YNL288W)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CHS7(YHR142W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COA4(YLR218C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COG6(YNL041C)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:5.37|P-value:3.90E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.92|P-value:4.28E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS10(YNR075W)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CWH43(YCR017C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DFM1(YDR411C)|FD-Score:3.43|P-value:2.96E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DOA1(YKL213C)|FD-Score:5.85|P-value:2.52E-9||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DUN1(YDL101C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:FET5(YFL041W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:GLR1(YPL091W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:HFI1(YPL254W)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HHT1(YBR010W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HPA2(YPR193C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HUA2(YOR284W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IRC16(YPR038W_d)|FD-Score:4.8|P-value:7.92E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC3(YDR332W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:7.26|P-value:1.99E-13||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LHS1(YKL073W)|FD-Score:-4.65|P-value:1.64E-6||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MIR1(YJR077C)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MLH1(YMR167W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MON2(YNL297C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NHP6A(YPR052C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:OSH6(YKR003W)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PDC6(YGR087C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PER33(YLR064W)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PHO88(YBR106W)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMT7(YDR307W_p)|FD-Score:4.47|P-value:3.87E-6||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PNC1(YGL037C)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress Gene:PUB1(YNL016W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RIC1(YLR039C)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL26A(YLR344W)|FD-Score:6.42|P-value:6.63E-11||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:5.4|P-value:3.30E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RXT3(YDL076C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SIF2(YBR103W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SIP5(YMR140W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SIS2(YKR072C)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLO1(YER180C-A)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SOD1(YJR104C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STM1(YLR150W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SWT1(YOR166C)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:SYS1(YJL004C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TPM1(YNL079C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:UBP14(YBR058C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:VAN1(YML115C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VPS61(YDR136C_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL018C(YAL018C_p)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.29|P-value:9.10E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBR134W(YBR134W_d)|FD-Score:5.57|P-value:1.30E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCK1(YHR135C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YDR010C(YDR010C_d)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR209C(YDR209C_d)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YGL010W(YGL010W_p)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR169C-A(YGR169C-A_p)|FD-Score:5.43|P-value:2.78E-8||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YIL012W(YIL012W_d)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL102C(YIL102C_p)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Putative protein of unknown function Gene:YJR038C(YJR038C_d)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR070W(YKR070W_p)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL047W(YLL047W_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YML007C-A(YML007C-A_p)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YOR338W(YOR338W_p)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:AAD3(YCR107W)|FD-Score:-4.61|P-value:2.06E-6||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AGP3(YFL055W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:AIF1(YNR074C)|FD-Score:5.77|P-value:3.88E-9||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:APL3(YBL037W)|FD-Score:9.33|P-value:5.12E-21||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:APL5(YPL195W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APM3(YBR288C)|FD-Score:5.69|P-value:6.24E-9||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:AZR1(YGR224W)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BNR1(YIL159W)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BTS1(YPL069C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF40(YNL288W)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CHS7(YHR142W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COA4(YLR218C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COG6(YNL041C)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:5.37|P-value:3.90E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.92|P-value:4.28E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS10(YNR075W)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CWH43(YCR017C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DFM1(YDR411C)|FD-Score:3.43|P-value:2.96E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DOA1(YKL213C)|FD-Score:5.85|P-value:2.52E-9||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DUN1(YDL101C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:FET5(YFL041W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:GLR1(YPL091W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:HFI1(YPL254W)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HHT1(YBR010W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HPA2(YPR193C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HUA2(YOR284W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IRC16(YPR038W_d)|FD-Score:4.8|P-value:7.92E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC3(YDR332W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:7.26|P-value:1.99E-13||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LHS1(YKL073W)|FD-Score:-4.65|P-value:1.64E-6||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MIR1(YJR077C)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MLH1(YMR167W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MON2(YNL297C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NHP6A(YPR052C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:OSH6(YKR003W)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PDC6(YGR087C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PER33(YLR064W)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PHO88(YBR106W)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMT7(YDR307W_p)|FD-Score:4.47|P-value:3.87E-6||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PNC1(YGL037C)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress Gene:PUB1(YNL016W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RIC1(YLR039C)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL26A(YLR344W)|FD-Score:6.42|P-value:6.63E-11||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:5.4|P-value:3.30E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RXT3(YDL076C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SIF2(YBR103W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SIP5(YMR140W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SIS2(YKR072C)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLO1(YER180C-A)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SOD1(YJR104C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STM1(YLR150W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SWT1(YOR166C)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:SYS1(YJL004C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TPM1(YNL079C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:UBP14(YBR058C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:VAN1(YML115C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VPS61(YDR136C_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL018C(YAL018C_p)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.29|P-value:9.10E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBR134W(YBR134W_d)|FD-Score:5.57|P-value:1.30E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCK1(YHR135C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YDR010C(YDR010C_d)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR209C(YDR209C_d)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YGL010W(YGL010W_p)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR169C-A(YGR169C-A_p)|FD-Score:5.43|P-value:2.78E-8||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YIL012W(YIL012W_d)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL102C(YIL102C_p)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Putative protein of unknown function Gene:YJR038C(YJR038C_d)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR070W(YKR070W_p)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL047W(YLL047W_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YML007C-A(YML007C-A_p)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YOR338W(YOR338W_p)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL014W4.386.01E-60.58CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YOR362C4.231.14E-50.58PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YOR256C4.231.16E-50.58TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YMR227C3.983.45E-50.58TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YKL186C3.403.38E-40.02MTR2mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
YHR042W3.383.64E-40.13NCP1NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
YJL019W3.255.87E-40.08MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YGR158C3.167.76E-40.05MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YBL023C3.129.17E-40.06MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YML031W3.060.001110.01NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YHR122W3.050.001140.11CIA2Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
YMR028W2.940.001620.06TAP42Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
YPR181C2.890.001950.12SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YKR037C2.760.002870.01SPC34Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YBR109C2.750.002980.02CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL037W9.335.12E-21APL3Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport
YKR019C7.261.99E-13IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YLR344W6.426.63E-11RPL26ARibosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
YLR261C_d5.941.40E-9VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YKL213C5.852.52E-9DOA1WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
YNR074C5.773.88E-9AIF1Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase
YBR288C5.696.24E-9APM3Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
YBR134W_d5.571.30E-8YBR134W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR169C-A_p5.432.78E-8YGR169C-A_pPutative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication
YDL020C5.403.30E-8RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YGL005C5.373.90E-8COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YIL012W_d5.141.40E-7YIL012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YNL288W5.131.43E-7CAF40Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p
YML071C4.924.28E-7COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YPR038W_d4.807.92E-7IRC16_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci

GO enrichment analysis for SGTC_2030
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1865.02E-47SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.134615Golgi
0.1611.19E-35SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.208333
0.1512.81E-31SGTC_1078idebenone 36.1 μMMiscellaneous36860.0545455Golgi
0.1482.90E-30SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.105263Golgi
0.1476.17E-30SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.122449RSC complex & mRNA processing
0.1471.04E-29SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.115942Golgi
0.1441.36E-28SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0892857Golgi
0.1381.22E-26SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0617284Golgi
0.1381.49E-26SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.2Golgi
0.1343.99E-25SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.151515Golgi
0.1289.54E-23SGTC_40960-0234 25.7 μMChemDiv (Drug-like library)68211120.144928Golgi
0.1261.90E-22SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.145833Golgi
0.1263.01E-22SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.131148Golgi
0.1231.88E-21SGTC_23469031566 143.8 μMChembridge (Fragment library)47710860.0816327Golgi
0.1226.09E-21SGTC_15324',5'-dihydroxyflavone 78.7 μMTimTec (Pure natural product library)1655210.196078Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20295175081200 μM0.4736841380876Chembridge (Fragment library)174.19922.12223
SGTC_18233-hydroxy-2-naphthoic acid106 μM0.37104TimTec (Natural product derivative library)188.179422.12623
SGTC_2235salicylic acid500 μM0.297297338Miscellaneous138.120741.21723
SGTC_10420350-009682 μM0.2765963122515ChemDiv (Drug-like library)372.41653.28825TSC3-RPN4
SGTC_2978901178442.97 μM0.2692312994934Chembridge (Drug-like library)309.38223.67423
SGTC_23027771753200 μM0.2666672203047Chembridge (Fragment library)217.286842.13412
SGTC_2116551145216.88 μM0.261905870744Chembridge (Fragment library)161.157381.53223
SGTC_9443370-0224231 μM0.264580514ChemDiv (Drug-like library)253.252742.96723
SGTC_2671danthron100 μM0.256412950Microsource (Natural product library)240.210922.32424DNA damage response
SGTC_23559061122103.12 μM0.2553195302957Chembridge (Fragment library)215.247822.07312