5185112

(5Z)-3-(hydroxymethyl)-5-(thiophen-2-ylmethylidene)-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2032
Screen concentration 26.0 μM
Source Chembridge (Fragment library)
PubChem CID 5718189
SMILES C1=CSC(=C1)C=C2C(=O)N(C(=O)S2)CO
Standardized SMILES OCN1C(=O)SC(=Cc2cccs2)C1=O
Molecular weight 241.2868
ALogP 1.54
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.96
% growth inhibition (Hom. pool) 4.9


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5718189
Download HIP data (tab-delimited text)  (excel)
Gene:RPT3(YDR394W)|FD-Score:-3.14|P-value:8.41E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RSC8(YFR037C)|FD-Score:-3.16|P-value:7.98E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC16(YPL085W)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.72||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:YPR177C(YPR177C_d)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:RPT3(YDR394W)|FD-Score:-3.14|P-value:8.41E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RSC8(YFR037C)|FD-Score:-3.16|P-value:7.98E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC16(YPL085W)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.72||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:YPR177C(YPR177C_d)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5718189
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:CBP2(YHL038C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:COA4(YLR218C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:CWH41(YGL027C)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:FLC3(YGL139W)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GCN4(YEL009C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GSM1(YJL103C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:GZF3(YJL110C)|FD-Score:-4.85|P-value:6.03E-7||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HMS2(YJR147W)|FD-Score:-3.73|P-value:9.42E-5||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HPA2(YPR193C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HST2(YPL015C)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IRC15(YPL017C)|FD-Score:5.01|P-value:2.73E-7||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ITR1(YDR497C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:MLH3(YPL164C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MNN2(YBR015C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPL10(YNL284C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:NPP2(YEL016C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:OPY2(YPR075C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PKR1(YMR123W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMP2(YEL017C-A)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PRM7(YDL039C)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:RAD7(YJR052W)|FD-Score:-4.28|P-value:9.46E-6||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RPL14A(YKL006W)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:4.34|P-value:7.29E-6||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SAE2(YGL175C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SFB3(YHR098C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SKY1(YMR216C)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SSA1(YAL005C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils Gene:TAL1(YLR354C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TRF5(YNL299W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRP1(YDR007W)|FD-Score:6.27|P-value:1.83E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:7.59|P-value:1.55E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR008C(YDR008C_d)|FD-Score:6.78|P-value:6.05E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR121W-A(YGR121W-A_p)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Putative protein of unknown function Gene:YJL022W(YJL022W_d)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJL118W(YJL118W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YJR037W(YJR037W_d)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKL077W(YKL077W_p)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR194C(YLR194C)|FD-Score:4.79|P-value:8.39E-7||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR280C(YLR280C_d)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR402W(YLR402W_d)|FD-Score:-3.84|P-value:6.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR166C(YMR166C_p)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YPL114W(YPL114W_d)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPL119C-A(YPL119C-A_p)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:ZEO1(YOL109W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:ACB1(YGR037C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:CBP2(YHL038C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:COA4(YLR218C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:CWH41(YGL027C)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:FLC3(YGL139W)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GCN4(YEL009C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GSM1(YJL103C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:GZF3(YJL110C)|FD-Score:-4.85|P-value:6.03E-7||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HMS2(YJR147W)|FD-Score:-3.73|P-value:9.42E-5||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HPA2(YPR193C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HST2(YPL015C)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IRC15(YPL017C)|FD-Score:5.01|P-value:2.73E-7||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ITR1(YDR497C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:MLH3(YPL164C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MNN2(YBR015C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPL10(YNL284C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:NPP2(YEL016C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:OPY2(YPR075C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PKR1(YMR123W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PMP2(YEL017C-A)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PRM7(YDL039C)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:RAD7(YJR052W)|FD-Score:-4.28|P-value:9.46E-6||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RPL14A(YKL006W)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:4.34|P-value:7.29E-6||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SAE2(YGL175C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SFB3(YHR098C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SKY1(YMR216C)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SSA1(YAL005C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils Gene:TAL1(YLR354C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TRF5(YNL299W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRP1(YDR007W)|FD-Score:6.27|P-value:1.83E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:7.59|P-value:1.55E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR008C(YDR008C_d)|FD-Score:6.78|P-value:6.05E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR121W-A(YGR121W-A_p)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Putative protein of unknown function Gene:YJL022W(YJL022W_d)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJL118W(YJL118W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YJR037W(YJR037W_d)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKL077W(YKL077W_p)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR194C(YLR194C)|FD-Score:4.79|P-value:8.39E-7||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR280C(YLR280C_d)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR402W(YLR402W_d)|FD-Score:-3.84|P-value:6.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR166C(YMR166C_p)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YPL114W(YPL114W_d)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPL119C-A(YPL119C-A_p)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:ZEO1(YOL109W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL085W3.641.34E-40.72SEC16COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
YAL032C2.920.001730.05PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YJL203W2.880.002010.12PRP21Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YGR280C2.760.002900.07PXR1Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
YNR038W2.690.003560.01DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YKL152C2.680.003660.07GPM1Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
YLL035W2.610.004570.02GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
YIL142W2.590.004770.01CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR060W2.580.004920.02FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YIL019W2.560.005190.05FAF1Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
YPR048W2.510.006070.03TAH18Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance
YDR167W2.470.006690.06TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YDL141W2.410.007890.04BPL1Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
YPL126W2.380.008760.04NAN1U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
YER036C2.330.009810.01ARB1ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR354W7.591.55E-14TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR008C_d6.786.05E-12YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR007W6.271.83E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YKL006W5.511.83E-8RPL14ARibosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
YPL017C5.012.73E-7IRC15Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
YLR194C4.798.39E-7YLR194CStructural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress
YEL009C4.542.85E-6GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YMR194W4.347.29E-6RPL36ARibosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
YKL077W_p4.102.08E-5YKL077W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YPR075C3.973.66E-5OPY2Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
YMR216C3.934.31E-5SKY1SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
YGR121W-A_p3.855.92E-5YGR121W-A_pPutative protein of unknown function
YPL114W_d3.581.69E-4YPL114W_dDubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index
YPR193C3.581.75E-4HPA2Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity
YPL015C3.532.12E-4HST2Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export

GO enrichment analysis for SGTC_2032
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3072.97E-129SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.456522
0.2706.56E-99SGTC_24885268135 45.7 μMMiscellaneous12703560.175439
0.2691.77E-98SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.169231
0.2688.19E-98SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0327869
0.2391.18E-77SGTC_500929-0078 56.4 μMChemDiv (Drug-like library)13777510.181818
0.2372.86E-76SGTC_18825615643 20.0 μMMiscellaneous22530750.231884TRP & mitochondrial translation
0.2361.01E-75SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.211538TRP & mitochondrial translation
0.2356.13E-75SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.166667
0.2342.21E-74SGTC_24865283202 22.4 μMMiscellaneous13777510.181818
0.2327.68E-73SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0641026
0.2328.94E-73SGTC_18815652484 16.0 μMMiscellaneous22551290.3125TRP & mitochondrial translation
0.2311.68E-72SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0735294
0.2294.42E-71SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.047619
0.2265.22E-69SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0897436
0.2224.65E-67SGTC_24785763493 32.3 μMMiscellaneous13655760.142857

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2448518511227.17 μM15718189Chembridge (Fragment library)241.286781.54415
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.4565221241132ChemDiv (Drug-like library)227.32642.79414
SGTC_2241677262537.81 μM0.3921572285411Chembridge (Fragment library)259.280463.09814
SGTC_21365328288199.3 μM0.377778262395Chembridge (Fragment library)222.220540.81724
SGTC_1434032-119415.74 μM0.370371806963ChemDiv (Drug-like library)329.3454833.69515
SGTC_2450573973712.93 μM0.3636362262825Miscellaneous328.3607233.79315
SGTC_2453573113222.18 μM0.3636361362040Miscellaneous436.266794.16614
SGTC_2065522159634.26 μM0.361702788581Chembridge (Fragment library)238.286141.71724
SGTC_1922557818725 μM0.3389831350412Miscellaneous362.8057834.45715TRP & mitochondrial translation
SGTC_2433560787427.82 μM0.3225812252428Miscellaneous374.84134.23515