5154463

5-acetyl-2-amino-6-methyl-4-phenyl-4H-pyran-3-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2035
Screen concentration 94.6 μM
Source Chembridge (Fragment library)
PubChem CID 2769623
SMILES CC1=C(C(C(=C(O1)N)C#N)C2=CC=CC=C2)C(=O)C
Standardized SMILES CC(=O)C1=C(C)OC(=C(C#N)C1c2ccccc2)N
Molecular weight 254.2839
ALogP 1.49
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.21
% growth inhibition (Hom. pool) 2.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2769623
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:3.12|P-value:8.95E-4|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB2(YIL083C)|FD-Score:5.43|P-value:2.88E-8|Clearance:0.55||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT3(YJL014W)|FD-Score:4.72|P-value:1.19E-6|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.29||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.79|P-value:7.44E-5|Clearance:0.21||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DPB2(YPR175W)|FD-Score:4.45|P-value:4.25E-6|Clearance:0.01||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:ECO1(YFR027W)|FD-Score:4.09|P-value:2.16E-5|Clearance:0||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG26(YGL001C)|FD-Score:3.91|P-value:4.69E-5|Clearance:0.11||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FBA1(YKL060C)|FD-Score:3.09|P-value:9.90E-4|Clearance:0.09||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:NAB2(YGL122C)|FD-Score:3.1|P-value:9.80E-4|Clearance:0||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NBP35(YGL091C)|FD-Score:4.44|P-value:4.41E-6|Clearance:0.29||SGD DESC:Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases Gene:NOP7(YGR103W)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:PAB1(YER165W)|FD-Score:4.09|P-value:2.16E-5|Clearance:0.18||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:RAP1(YNL216W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.03||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RET1(YOR207C)|FD-Score:5.98|P-value:1.12E-9|Clearance:0.55||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB8(YOR224C)|FD-Score:-4.06|P-value:2.41E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPP1(YHR062C)|FD-Score:7.89|P-value:1.50E-15|Clearance:1.91||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SGT1(YOR057W)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.18||SGD DESC:Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress Gene:SPC3(YLR066W)|FD-Score:4.87|P-value:5.46E-7|Clearance:0.16||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SSL2(YIL143C)|FD-Score:-4.26|P-value:1.00E-5|Clearance:0||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:SWI1(YPL016W)|FD-Score:3.1|P-value:9.61E-4|Clearance:0.01||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAH18(YPR048W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.07||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:YJL009W(YJL009W_d)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YNL114C(YNL114C_d)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YRB2(YIL063C)|FD-Score:3.23|P-value:6.12E-4|Clearance:0.04||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential Gene:ARP7(YPR034W)|FD-Score:3.12|P-value:8.95E-4|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB2(YIL083C)|FD-Score:5.43|P-value:2.88E-8|Clearance:0.55||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT3(YJL014W)|FD-Score:4.72|P-value:1.19E-6|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.29||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.79|P-value:7.44E-5|Clearance:0.21||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DPB2(YPR175W)|FD-Score:4.45|P-value:4.25E-6|Clearance:0.01||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:ECO1(YFR027W)|FD-Score:4.09|P-value:2.16E-5|Clearance:0||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG26(YGL001C)|FD-Score:3.91|P-value:4.69E-5|Clearance:0.11||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FBA1(YKL060C)|FD-Score:3.09|P-value:9.90E-4|Clearance:0.09||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:NAB2(YGL122C)|FD-Score:3.1|P-value:9.80E-4|Clearance:0||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NBP35(YGL091C)|FD-Score:4.44|P-value:4.41E-6|Clearance:0.29||SGD DESC:Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases Gene:NOP7(YGR103W)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:PAB1(YER165W)|FD-Score:4.09|P-value:2.16E-5|Clearance:0.18||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:RAP1(YNL216W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.03||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RET1(YOR207C)|FD-Score:5.98|P-value:1.12E-9|Clearance:0.55||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB8(YOR224C)|FD-Score:-4.06|P-value:2.41E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPP1(YHR062C)|FD-Score:7.89|P-value:1.50E-15|Clearance:1.91||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SGT1(YOR057W)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.18||SGD DESC:Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress Gene:SPC3(YLR066W)|FD-Score:4.87|P-value:5.46E-7|Clearance:0.16||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SSL2(YIL143C)|FD-Score:-4.26|P-value:1.00E-5|Clearance:0||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:SWI1(YPL016W)|FD-Score:3.1|P-value:9.61E-4|Clearance:0.01||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAH18(YPR048W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.07||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:YJL009W(YJL009W_d)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YNL114C(YNL114C_d)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YRB2(YIL063C)|FD-Score:3.23|P-value:6.12E-4|Clearance:0.04||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2769623
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AST2(YER101C)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:BAT2(YJR148W)|FD-Score:-3.8|P-value:7.35E-5||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BDF2(YDL070W)|FD-Score:4.28|P-value:9.47E-6||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIN2(YPL241C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:7.44|P-value:5.02E-14||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CNM67(YNL225C)|FD-Score:4.48|P-value:3.82E-6||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COA4(YLR218C)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COX19(YLL018C-A)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CRS5(YOR031W)|FD-Score:6.13|P-value:4.48E-10||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DBR1(YKL149C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DTD1(YDL219W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:EPS1(YIL005W)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERV41(YML067C)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FCJ1(YKR016W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GIR2(YDR152W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GYP1(YOR070C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:MHF2(YDL160C-A)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MNN2(YBR015C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOS2(YGR235C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MPM1(YJL066C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MSA1(YOR066W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSE1(YOL033W)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:NDJ1(YOL104C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NIT2(YJL126W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NRT1(YOR071C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:NUP120(YKL057C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:PDR15(YDR406W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PET20(YPL159C)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PML39(YML107C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PPQ1(YPL179W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:RAD26(YJR035W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RAD52(YML032C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RAS1(YOR101W)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL19A(YBR084C-A)|FD-Score:4.83|P-value:6.92E-7||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPN4(YDL020C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS9B(YBR189W)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:SFA1(YDL168W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SKN1(YGR143W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SPO75(YLL005C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SPR28(YDR218C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:SPT23(YKL020C)|FD-Score:4.27|P-value:9.99E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SWE1(YJL187C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:SWF1(YDR126W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TOM1(YDR457W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TPO1(YLL028W)|FD-Score:4|P-value:3.22E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:4.89|P-value:4.99E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA3(YDL170W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL065W(YBL065W_d)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YBR074W(YBR074W_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Putative metalloprotease Gene:YCL012C(YCL012C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDL241W(YDL241W_p)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YGR022C(YGR022C_d)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Gene:YLR179C(YLR179C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus Gene:YNL092W(YNL092W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNR048W(YNR048W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YNR062C(YNR062C_p)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Putative membrane protein of unknown function Gene:ADO1(YJR105W)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AST2(YER101C)|FD-Score:3.74|P-value:9.13E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:BAT2(YJR148W)|FD-Score:-3.8|P-value:7.35E-5||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BDF2(YDL070W)|FD-Score:4.28|P-value:9.47E-6||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIN2(YPL241C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:7.44|P-value:5.02E-14||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CNM67(YNL225C)|FD-Score:4.48|P-value:3.82E-6||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COA4(YLR218C)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COX19(YLL018C-A)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CRS5(YOR031W)|FD-Score:6.13|P-value:4.48E-10||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DBR1(YKL149C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DTD1(YDL219W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:EPS1(YIL005W)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERV41(YML067C)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FCJ1(YKR016W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GIR2(YDR152W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GYP1(YOR070C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:MHF2(YDL160C-A)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MNN2(YBR015C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOS2(YGR235C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MPM1(YJL066C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MSA1(YOR066W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSE1(YOL033W)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:NDJ1(YOL104C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NIT2(YJL126W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NRT1(YOR071C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:NUP120(YKL057C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:PDR15(YDR406W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PET20(YPL159C)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PML39(YML107C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PPQ1(YPL179W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:RAD26(YJR035W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RAD52(YML032C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RAS1(YOR101W)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL19A(YBR084C-A)|FD-Score:4.83|P-value:6.92E-7||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPN4(YDL020C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS9B(YBR189W)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:SFA1(YDL168W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SKN1(YGR143W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SPO75(YLL005C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SPR28(YDR218C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:SPT23(YKL020C)|FD-Score:4.27|P-value:9.99E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SWE1(YJL187C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:SWF1(YDR126W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TOM1(YDR457W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TPO1(YLL028W)|FD-Score:4|P-value:3.22E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:4.89|P-value:4.99E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA3(YDL170W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL065W(YBL065W_d)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YBR074W(YBR074W_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Putative metalloprotease Gene:YCL012C(YCL012C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDL241W(YDL241W_p)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YGR022C(YGR022C_d)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Gene:YLR179C(YLR179C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus Gene:YNL092W(YNL092W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene Gene:YNR048W(YNR048W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YNR062C(YNR062C_p)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Putative membrane protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C7.891.50E-151.91RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR207C5.981.12E-90.55RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YIL083C5.432.88E-80.55CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YLR066W4.875.46E-70.16SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YJL014W4.721.19E-60.09CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YOR057W4.631.85E-60.17SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
YPR175W4.454.25E-60.01DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YGL091C4.444.41E-60.29NBP35Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
YJL009W_d4.151.66E-50.06YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YFR027W4.092.16E-52.07E-4ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YER165W4.092.16E-50.18PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YGL001C3.914.69E-50.11ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YJL111W3.797.44E-50.21CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YNL216W3.591.68E-40.03RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YJR064W3.561.88E-40.29CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL298W7.445.02E-14CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YOR031W6.134.48E-10CRS5Copper-binding metallothionein, required for wild-type copper resistance
YLR218C5.062.11E-7COA4Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors
YBR058C-A4.894.99E-7TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YBR084C-A4.836.92E-7RPL19ARibosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
YKL149C4.741.06E-6DBR1RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition
YDL241W_p4.641.76E-6YDL241W_pPutative protein of unknown function; YDL241W is not an essential gene
YNL225C4.483.82E-6CNM67Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication
YDL070W4.289.47E-6BDF2Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress
YKL020C4.279.99E-6SPT23ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting
YBR015C4.241.11E-5MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YDR406W4.211.28E-5PDR15Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
YML032C4.121.89E-5RAD52Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis
YLL028W4.003.22E-5TPO1Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane
YJL187C3.944.09E-5SWE1Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate

GO enrichment analysis for SGTC_2035
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2381.41E-76SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.370968
0.2321.00E-72SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.132353
0.2173.69E-64SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.138462
0.1966.20E-52SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.189655TSC3-RPN4
0.1911.60E-49SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.126984TSC3-RPN4
0.1905.92E-49SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.12
0.1845.01E-46SGTC_21515563267 85.4 μMChembridge (Fragment library)53108240.0967742
0.1819.37E-45SGTC_5941487-1584 10.8 μMChemDiv (Drug-like library)50133320.0789474
0.1696.42E-39SGTC_120849-0099 9.3 μMChemDiv (Drug-like library)60713880.112676
0.1628.12E-36SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.116667TSC3-RPN4
0.1612.60E-35SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.12987
0.1604.74E-35SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.185185TSC3-RPN4
0.1555.81E-33SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.133333
0.1541.34E-32SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.166667
0.1541.16E-32SGTC_21135491891 107.0 μMChembridge (Fragment library)7899790.288462TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2067522462934.08 μM0.7021282835000Chembridge (Fragment library)288.728922.15414
SGTC_20705230947200 μM0.6666672835538Chembridge (Fragment library)260.311581.23215
SGTC_20865274770176.62 μM0.422222689278Chembridge (Fragment library)254.31031.46325
SGTC_1643st00986620.2 μM0.3709683156273TimTec (Natural product derivative library)320.298881.92616
SGTC_1662st01405452 μM0.3692312838777TimTec (Natural product derivative library)360.362742.92816TSC3-RPN4
SGTC_9221866-0035480 μM0.3620693156169ChemDiv (Drug-like library)305.373663.30924excess fatty acid
SGTC_13681852-0141284 μM0.3508772838987ChemDiv (Drug-like library)281.2843832.3225ERG2
SGTC_1978st07176142 μM0.355147680TimTec (Natural product derivative library)292.331843.56224TSC3-RPN4
SGTC_1719st03222749.2 μM0.3484853125804TimTec (Natural product derivative library)406.388122.54718
SGTC_20855307070144.05 μM0.338712840351Chembridge (Fragment library)286.348861.7415superoxide