5169046

(2E)-2-[(2-hydroxyethylamino)methylidene]-1H-indol-3-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2037
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 5718022
SMILES C1=CC=C2C(=C1)C(=O)C(=CNCCO)N2
Standardized SMILES OCCN=Cc1[nH]c2ccccc2c1O
Molecular weight 204.2252
ALogP -0.09
H-bond donor count 3
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.59
% growth inhibition (Hom. pool) 4.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5718022
Download HIP data (tab-delimited text)  (excel)
Gene:HEM12(YDR047W)|FD-Score:6.96|P-value:1.69E-12|Clearance:1.34||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:LSG1(YGL099W)|FD-Score:4.3|P-value:8.46E-6|Clearance:0.02||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MDN1(YLR106C)|FD-Score:3.12|P-value:8.89E-4|Clearance:0.16||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NMD3(YHR170W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.21||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP8(YOL144W)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.13||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:RPC82(YPR190C)|FD-Score:-5.01|P-value:2.77E-7|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RSC6(YCR052W)|FD-Score:5.62|P-value:9.37E-9|Clearance:1.32||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:4.28|P-value:9.23E-6|Clearance:0.54||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SPC105(YGL093W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.15||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:ULP2(YIL031W)|FD-Score:3.17|P-value:7.72E-4|Clearance:0.01||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:UTP6(YDR449C)|FD-Score:-3.12|P-value:9.07E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:3.75|P-value:8.96E-5|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:HEM12(YDR047W)|FD-Score:6.96|P-value:1.69E-12|Clearance:1.34||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:LSG1(YGL099W)|FD-Score:4.3|P-value:8.46E-6|Clearance:0.02||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MDN1(YLR106C)|FD-Score:3.12|P-value:8.89E-4|Clearance:0.16||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NMD3(YHR170W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.21||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP8(YOL144W)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.13||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:RPC82(YPR190C)|FD-Score:-5.01|P-value:2.77E-7|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RSC6(YCR052W)|FD-Score:5.62|P-value:9.37E-9|Clearance:1.32||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:4.28|P-value:9.23E-6|Clearance:0.54||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SPC105(YGL093W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.15||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:ULP2(YIL031W)|FD-Score:3.17|P-value:7.72E-4|Clearance:0.01||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:UTP6(YDR449C)|FD-Score:-3.12|P-value:9.07E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:3.75|P-value:8.96E-5|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5718022
Download HOP data (tab-delimited text)  (excel)
Gene:ADK2(YER170W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ALY2(YJL084C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ASF2(YDL197C)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Anti-silencing protein that causes derepression of silent loci when overexpressed Gene:BIM1(YER016W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BTN2(YGR142W)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CLN2(YPL256C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:DBR1(YKL149C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:ELG1(YOR144C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FUN14(YAL008W)|FD-Score:5.85|P-value:2.46E-9||SGD DESC:Mitochondrial protein of unknown function Gene:GEP3(YOR205C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GRE3(YHR104W)|FD-Score:4.94|P-value:3.98E-7||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GTT1(YIR038C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:HIS5(YIL116W)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:INP1(YMR204C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC21(YMR073C)|FD-Score:-4.6|P-value:2.08E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LHS1(YKL073W)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LTV1(YKL143W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MDJ1(YFL016C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MGT1(YDL200C)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MLF3(YNL074C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide Gene:MOH1(YBL049W)|FD-Score:4.28|P-value:9.34E-6||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:NCE102(YPR149W)|FD-Score:-4.49|P-value:3.53E-6||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:NCL1(YBL024W)|FD-Score:4|P-value:3.15E-5||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NGL2(YMR285C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:PBP4(YDL053C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PHM6(YDR281C)|FD-Score:4.36|P-value:6.47E-6||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:RPO41(YFL036W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:SDL1(YIL167W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:STB4(YMR019W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STE50(YCL032W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:TPO2(YGR138C)|FD-Score:-3.18|P-value:7.24E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication Gene:YAL065C(YAL065C_p)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBR074W(YBR074W_p)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Putative metalloprotease Gene:YDR290W(YDR290W_d)|FD-Score:-3.81|P-value:6.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YEA6(YEL006W)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YFR020W(YFR020W_p)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL041C-B(YGL041C-B_p)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGP1(YNL160W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YLR280C(YLR280C_d)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR316C-B(YMR316C-B_d)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR223W(YOR223W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YPK3(YBR028C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL067C(YPL067C_p)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL102C(YPL102C_d)|FD-Score:-4.03|P-value:2.78E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPL150W(YPL150W_p)|FD-Score:6.01|P-value:9.30E-10||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:YPR197C(YPR197C_d)|FD-Score:4|P-value:3.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSC84(YHR016C)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ADK2(YER170W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ALY2(YJL084C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ASF2(YDL197C)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Anti-silencing protein that causes derepression of silent loci when overexpressed Gene:BIM1(YER016W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BTN2(YGR142W)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CLN2(YPL256C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:DBR1(YKL149C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:ELG1(YOR144C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FUN14(YAL008W)|FD-Score:5.85|P-value:2.46E-9||SGD DESC:Mitochondrial protein of unknown function Gene:GEP3(YOR205C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GRE3(YHR104W)|FD-Score:4.94|P-value:3.98E-7||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GTT1(YIR038C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:HIS5(YIL116W)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:INP1(YMR204C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC21(YMR073C)|FD-Score:-4.6|P-value:2.08E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LHS1(YKL073W)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LTV1(YKL143W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MDJ1(YFL016C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MGT1(YDL200C)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MLF3(YNL074C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide Gene:MOH1(YBL049W)|FD-Score:4.28|P-value:9.34E-6||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:NCE102(YPR149W)|FD-Score:-4.49|P-value:3.53E-6||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:NCL1(YBL024W)|FD-Score:4|P-value:3.15E-5||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NGL2(YMR285C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:PBP4(YDL053C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PHM6(YDR281C)|FD-Score:4.36|P-value:6.47E-6||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:RPO41(YFL036W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:SDL1(YIL167W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:STB4(YMR019W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STE50(YCL032W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:TPO2(YGR138C)|FD-Score:-3.18|P-value:7.24E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication Gene:YAL065C(YAL065C_p)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBR074W(YBR074W_p)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Putative metalloprotease Gene:YDR290W(YDR290W_d)|FD-Score:-3.81|P-value:6.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YEA6(YEL006W)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YFR020W(YFR020W_p)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL041C-B(YGL041C-B_p)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGP1(YNL160W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YLR280C(YLR280C_d)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR316C-B(YMR316C-B_d)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR223W(YOR223W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YPK3(YBR028C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL067C(YPL067C_p)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL102C(YPL102C_d)|FD-Score:-4.03|P-value:2.78E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPL150W(YPL150W_p)|FD-Score:6.01|P-value:9.30E-10||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:YPR197C(YPR197C_d)|FD-Score:4|P-value:3.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSC84(YHR016C)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR047W6.961.69E-121.34HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YCR052W5.629.37E-91.32RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YGL099W4.308.46E-60.02LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YFR037C4.289.23E-60.54RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDL196W_d3.758.96E-50.10YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YHR170W3.651.31E-40.20NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YOL144W3.442.86E-40.13NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YGL093W3.324.57E-40.15SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YIL031W3.177.72E-40.01ULP2Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate
YJL086C_d3.158.11E-40.03YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YLR106C3.128.89E-40.16MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
YDL205C2.960.001530.20HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YML127W2.760.002870.07RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YEL035C_p2.700.003500.03UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YJL156C2.670.003800.04SSY5Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL150W_p6.019.30E-10YPL150W_pProtein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study
YAL008W5.852.46E-9FUN14Mitochondrial protein of unknown function
YHR104W4.943.98E-7GRE3Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
YHR016C4.731.11E-6YSC84Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
YDL197C4.711.25E-6ASF2Anti-silencing protein that causes derepression of silent loci when overexpressed
YGL041C-B_p4.711.25E-6YGL041C-B_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YIL116W4.592.18E-6HIS5Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
YDR281C4.366.47E-6PHM6Protein of unknown function, expression is regulated by phosphate levels
YBL049W4.289.34E-6MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YKL073W4.279.75E-6LHS1Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
YOR223W4.221.24E-5YOR223WSubunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3
YBR074W_p4.042.68E-5YBR074W_pPutative metalloprotease
YPR197C_d4.003.14E-5YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBL024W4.003.15E-5NCL1S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
YFL016C3.993.28E-5MDJ1Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones

GO enrichment analysis for SGTC_2037
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1576.17E-34SGTC_273hydroxyurea 18.1 mMMiscellaneous36570.0731707heme biosynthesis & mitochondrial translocase
0.1382.28E-26SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.148148heme biosynthesis & mitochondrial translocase
0.1373.43E-26SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.092307760S ribosome export
0.1264.25E-22SGTC_10258-0098 57.4 μMChemDiv (Drug-like library)67900320.0857143heme biosynthesis & mitochondrial translocase
0.1141.26E-18SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.160S ribosome export
0.1132.58E-18SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.114754heme biosynthesis & mitochondrial translocase
0.1111.26E-17SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.125copper-dependent oxidative stress
0.1111.65E-17SGTC_10573448-5381 134.0 μMChemDiv (Drug-like library)54979110.133333calcium & mitochondrial duress
0.1088.41E-17SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0441176heme biosynthesis & mitochondrial translocase
0.1081.13E-16SGTC_20014023502 57.7 μMChembridge (Fragment library)1089410.134615mitochondrial processes
0.1049.78E-16SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.0757576mitochondrial processes
0.1041.04E-15SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.070588260S ribosome export
0.1024.18E-15SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.053333360S ribosome export
0.1017.67E-15SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.0597015heme biosynthesis & mitochondrial translocase
0.0992.16E-14SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.11290360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_501nsc-95397161 μM0.325581262093ICCB bioactive library310.388561.17126
SGTC_13772944-0135198 μM0.2857141894137ChemDiv (Drug-like library)325.404822.47332calcium & mitochondrial duress
SGTC_21085365811200 μM0.282609780283Chembridge (Fragment library)213.231941.21213
SGTC_900870-08634.61 μM0.2807021738164ChemDiv (Drug-like library)311.762263.53812
SGTC_289k017-0060526 μM0.2678575512909ChemDiv (Drug-like library)380.21651.56725
SGTC_1520flindersine88 μM0.26415168230TimTec (Pure natural product library)227.258521.70412
SGTC_2706st07823982.5 μM0.26415115434492TimTec (Natural product derivative library)242.273161.08712
SGTC_1468k284-1086134 μM0.26229515996457ChemDiv (Drug-like library)360.453842.80634DNA intercalators
SGTC_7783456-2434196 μM0.2592595539063ChemDiv (Drug-like library)235.259061.88415mitochondrial response to ROS
SGTC_219mitoxanthrone164.41 μM0.2549024212Miscellaneous444.480920.068810DNA intercalators