1543-0184

(5E)-3-[(2,5-dimethylanilino)methyl]-5-[(2-fluorophenyl)methylidene]-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_204
Screen concentration 62.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 2252213
SMILES CC1=CC(=C(C=C1)C)NCN2C(=O)C(=CC3=CC=CC=C3F)SC2=O
Standardized SMILES Cc1ccc(C)c(NCN2C(=O)SC(=Cc3ccccc3F)C2=O)c1
Molecular weight 356.4139
ALogP 4.77
H-bond donor count 1
H-bond acceptor count 5
Response signature TRP & mitochondrial translation

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.11
% growth inhibition (Hom. pool) 16.06


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2252213
Download HIP data (tab-delimited text)  (excel)
Gene:CHS2(YBR038W)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.07||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:DED1(YOR204W)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ERG11(YHR007C)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG12(YMR208W)|FD-Score:4.58|P-value:2.36E-6|Clearance:0.97||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:NUP192(YJL039C)|FD-Score:3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:NUS1(YDL193W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.13||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PMA1(YGL008C)|FD-Score:19.5|P-value:4.09E-85|Clearance:9.31||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RER2(YBR002C)|FD-Score:10.2|P-value:9.12E-25|Clearance:3.79||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPL15A(YLR029C)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:6.42|P-value:6.70E-11|Clearance:1.22||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC5(YDR166C)|FD-Score:5.2|P-value:9.87E-8|Clearance:0.16||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SEC62(YPL094C)|FD-Score:3.12|P-value:9.03E-4|Clearance:0.09||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPC42(YKL042W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.22||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TIM22(YDL217C)|FD-Score:5.04|P-value:2.29E-7|Clearance:0.47||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:VRG4(YGL225W)|FD-Score:3.39|P-value:3.54E-4|Clearance:0.03||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:CHS2(YBR038W)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.07||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:DED1(YOR204W)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ERG11(YHR007C)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG12(YMR208W)|FD-Score:4.58|P-value:2.36E-6|Clearance:0.97||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:NUP192(YJL039C)|FD-Score:3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:NUS1(YDL193W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.13||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PMA1(YGL008C)|FD-Score:19.5|P-value:4.09E-85|Clearance:9.31||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RER2(YBR002C)|FD-Score:10.2|P-value:9.12E-25|Clearance:3.79||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPL15A(YLR029C)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:6.42|P-value:6.70E-11|Clearance:1.22||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC5(YDR166C)|FD-Score:5.2|P-value:9.87E-8|Clearance:0.16||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SEC62(YPL094C)|FD-Score:3.12|P-value:9.03E-4|Clearance:0.09||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPC42(YKL042W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.22||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TIM22(YDL217C)|FD-Score:5.04|P-value:2.29E-7|Clearance:0.47||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:VRG4(YGL225W)|FD-Score:3.39|P-value:3.54E-4|Clearance:0.03||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2252213
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ALF1(YNL148C)|FD-Score:4.97|P-value:3.43E-7||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:AMN1(YBR158W)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:APN1(YKL114C)|FD-Score:4.95|P-value:3.81E-7||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG7(YMR062C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:BRP1(YGL007W_d)|FD-Score:11.8|P-value:2.19E-32||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BST1(YFL025C)|FD-Score:4.88|P-value:5.19E-7||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUB2(YMR055C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CEX1(YOR112W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CLC1(YGR167W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COS111(YBR203W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSI2(YOL007C)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene Gene:CTF19(YPL018W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CWH43(YCR017C)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:EMP70(YLR083C)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERD1(YDR414C)|FD-Score:6.12|P-value:4.76E-10||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG4(YGL012W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FRA2(YGL220W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GCN2(YDR283C)|FD-Score:4.38|P-value:5.98E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN4(YEL009C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HMI1(YOL095C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:INP54(YOL065C)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:IRC23(YOR044W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:KAR9(YPL269W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:MHO1(YJR008W)|FD-Score:-3.74|P-value:9.37E-5||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MHR1(YDR296W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MMM1(YLL006W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP21(YBL090W)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL16(YBL038W)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL35(YDR322W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:5.12|P-value:1.56E-7||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS5(YBR251W)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTO1(YGL236C)|FD-Score:4.27|P-value:9.90E-6||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:NOT3(YIL038C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OPI10(YOL032W)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDE1(YGL248W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PEX14(YGL153W)|FD-Score:-6.14|P-value:4.23E-10||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PEX17(YNL214W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PKR1(YMR123W)|FD-Score:5.22|P-value:8.81E-8||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POS5(YPL188W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:RAV1(YJR033C)|FD-Score:7.89|P-value:1.47E-15||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:6.24|P-value:2.14E-10||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RIM1(YCR028C-A)|FD-Score:3.74|P-value:9.02E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPS1A(YLR441C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SDH1(YKL148C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SFB3(YHR098C)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SKY1(YMR216C)|FD-Score:5.63|P-value:9.24E-9||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SWC5(YBR231C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SYF2(YGR129W)|FD-Score:-3.13|P-value:8.76E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRP1(YDR007W)|FD-Score:9.39|P-value:3.00E-21||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:9.6|P-value:4.12E-22||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.25|P-value:2.00E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBP6(YFR010W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VNX1(YNL321W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YCR090C(YCR090C_p)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.55|P-value:2.96E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:10.1|P-value:2.72E-24||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER175W-A(YER175W-A_p)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL007C-A(YGL007C-A_p)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YIL060W(YIL060W_p)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIP3(YNL044W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YKL077W(YKL077W_p)|FD-Score:8.08|P-value:3.37E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YML122C(YML122C_d)|FD-Score:5.91|P-value:1.70E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR209C(YMR209C_p)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR315W(YMR315W)|FD-Score:6.37|P-value:9.42E-11||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOR365C(YOR365C_p)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPL014W(YPL014W_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YRR1(YOR162C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ALF1(YNL148C)|FD-Score:4.97|P-value:3.43E-7||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:AMN1(YBR158W)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:APN1(YKL114C)|FD-Score:4.95|P-value:3.81E-7||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG7(YMR062C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:BRP1(YGL007W_d)|FD-Score:11.8|P-value:2.19E-32||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BST1(YFL025C)|FD-Score:4.88|P-value:5.19E-7||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUB2(YMR055C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CEX1(YOR112W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CLC1(YGR167W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COS111(YBR203W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSI2(YOL007C)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene Gene:CTF19(YPL018W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CWH43(YCR017C)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:EMP70(YLR083C)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERD1(YDR414C)|FD-Score:6.12|P-value:4.76E-10||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG4(YGL012W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FRA2(YGL220W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GCN2(YDR283C)|FD-Score:4.38|P-value:5.98E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN4(YEL009C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HMI1(YOL095C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:INP54(YOL065C)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:IRC23(YOR044W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:KAR9(YPL269W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:MHO1(YJR008W)|FD-Score:-3.74|P-value:9.37E-5||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MHR1(YDR296W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MMM1(YLL006W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP21(YBL090W)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL16(YBL038W)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL35(YDR322W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:5.12|P-value:1.56E-7||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS5(YBR251W)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTO1(YGL236C)|FD-Score:4.27|P-value:9.90E-6||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:NOT3(YIL038C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OPI10(YOL032W)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDE1(YGL248W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PEX14(YGL153W)|FD-Score:-6.14|P-value:4.23E-10||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PEX17(YNL214W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PKR1(YMR123W)|FD-Score:5.22|P-value:8.81E-8||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POS5(YPL188W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:RAV1(YJR033C)|FD-Score:7.89|P-value:1.47E-15||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:6.24|P-value:2.14E-10||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RIM1(YCR028C-A)|FD-Score:3.74|P-value:9.02E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPS1A(YLR441C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SDH1(YKL148C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SFB3(YHR098C)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SKY1(YMR216C)|FD-Score:5.63|P-value:9.24E-9||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SWC5(YBR231C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SYF2(YGR129W)|FD-Score:-3.13|P-value:8.76E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRP1(YDR007W)|FD-Score:9.39|P-value:3.00E-21||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:9.6|P-value:4.12E-22||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.25|P-value:2.00E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBP6(YFR010W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VNX1(YNL321W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YCR090C(YCR090C_p)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.55|P-value:2.96E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:10.1|P-value:2.72E-24||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER175W-A(YER175W-A_p)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL007C-A(YGL007C-A_p)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YIL060W(YIL060W_p)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIP3(YNL044W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p Gene:YKL077W(YKL077W_p)|FD-Score:8.08|P-value:3.37E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YML122C(YML122C_d)|FD-Score:5.91|P-value:1.70E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR209C(YMR209C_p)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR315W(YMR315W)|FD-Score:6.37|P-value:9.42E-11||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOR365C(YOR365C_p)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPL014W(YPL014W_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YRR1(YOR162C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL008C19.504.09E-859.31PMA1Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
YBR002C10.209.12E-253.79RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YHR062C6.426.70E-111.22RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR166C5.209.87E-80.16SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YDL217C5.042.29E-70.47TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YMR208W4.582.36E-60.97ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YKL042W3.611.55E-40.22SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YGL225W3.393.54E-40.03VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YLR029C3.354.01E-40.01RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YJL039C3.344.21E-40.00NUP192Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
YBR038W3.334.27E-40.07CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YHR007C3.265.54E-40.01ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDL193W3.255.69E-40.13NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YPL094C3.129.03E-40.09SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YPL251W_d3.030.001210.03YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL007W_d11.802.19E-32BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YDR203W_d10.102.72E-24YDR203W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL211C9.604.12E-22TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR007W9.393.00E-21TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YKL077W_p8.083.37E-16YKL077W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YJR033C7.891.47E-15RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YDR008C_d6.552.96E-11YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR315W6.379.42E-11YMR315WProtein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress
YDR354W6.252.00E-10TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR202C6.242.14E-10RAV2Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
YDR414C6.124.76E-10ERD1Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
YML122C_d5.911.70E-9YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR216C5.639.24E-9SKY1SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
YMR123W5.228.81E-8PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YNR036C5.121.56E-7MRPS12Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins

GO enrichment analysis for SGTC_204
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3898.78E-213SGTC_18815652484 16.0 μMMiscellaneous22551290.644068TRP & mitochondrial translation
0.3862.85E-209SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.148649TRP & mitochondrial translation
0.3757.83E-196SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.1875TRP & mitochondrial translation
0.3613.08E-181SGTC_500929-0078 56.4 μMChemDiv (Drug-like library)13777510.164179
0.3472.22E-166SGTC_8920929-0076 29.0 μMChemDiv (Drug-like library)57510380.225806TRP & mitochondrial translation
0.3225.30E-142SGTC_19215740136 50.0 μMMiscellaneous17742430.516129TRP & mitochondrial translation
0.3128.55E-134SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.283784
0.3004.56E-123SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0735294TRP & mitochondrial translation
0.2963.22E-119SGTC_24885268135 45.7 μMMiscellaneous12703560.15942
0.2913.33E-115SGTC_18825615643 20.0 μMMiscellaneous22530750.380282TRP & mitochondrial translation
0.2793.12E-106SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.0735294TRP & mitochondrial translation
0.2773.23E-104SGTC_19225578187 25.0 μMMiscellaneous13504120.672727TRP & mitochondrial translation
0.2762.72E-103SGTC_24545-benzylidenerhodanine 10.4 μMMiscellaneous12732100.1875TRP & mitochondrial translation
0.2755.29E-103SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.152778
0.2571.10E-89SGTC_24865283202 22.4 μMMiscellaneous13777510.164179

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1922557818725 μM0.6727271350412Miscellaneous362.8057834.45715TRP & mitochondrial translation
SGTC_1881565248416 μM0.6440682255129Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_2426558204632.21 μM0.5714292251934Miscellaneous386.3968033.64817
SGTC_2450573973712.93 μM0.5714292262825Miscellaneous328.3607233.79315
SGTC_24375741531144.16 μM0.5384622262990Miscellaneous402.4392634.24617
SGTC_1921574013650 μM0.5161291774243Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_2433560787427.82 μM0.5079372252428Miscellaneous374.84134.23515
SGTC_2445574259735.57 μM0.4428571774255Miscellaneous433.318884.80515
SGTC_2447574174233.78 μM0.4411761776504Miscellaneous433.318884.80515
SGTC_2453573113222.18 μM0.4193551362040Miscellaneous436.266794.16614