4141938

methyl 3-amino-5-fluorobenzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2047
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 3807862
SMILES COC(=O)C1=CC(=CC(=C1)F)N
Standardized SMILES COC(=O)c1cc(N)cc(F)c1
Molecular weight 169.153
ALogP 1.14
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.6
% growth inhibition (Hom. pool) 4.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3807862
Download HIP data (tab-delimited text)  (excel)
Gene:ALG13(YGL047W)|FD-Score:-4.02|P-value:2.88E-5|Clearance:0||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.07||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC3(YLR314C)|FD-Score:-3.18|P-value:7.49E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:ERG29(YMR134W_p)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:MED8(YBR193C)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.18||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOG1(YPL093W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.11||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:OLE1(YGL055W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PRP22(YER013W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RPC53(YDL150W)|FD-Score:3.94|P-value:4.00E-5|Clearance:0.25||SGD DESC:RNA polymerase III subunit C53 Gene:RRP46(YGR095C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.1||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SPC42(YKL042W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:YLR140W(YLR140W_d)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:ALG13(YGL047W)|FD-Score:-4.02|P-value:2.88E-5|Clearance:0||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.07||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC3(YLR314C)|FD-Score:-3.18|P-value:7.49E-4|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:ERG29(YMR134W_p)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:MED8(YBR193C)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.18||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOG1(YPL093W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.11||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:OLE1(YGL055W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PRP22(YER013W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RPC53(YDL150W)|FD-Score:3.94|P-value:4.00E-5|Clearance:0.25||SGD DESC:RNA polymerase III subunit C53 Gene:RRP46(YGR095C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.1||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SPC42(YKL042W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:YLR140W(YLR140W_d)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3807862
Download HOP data (tab-delimited text)  (excel)
Gene:BSC1(YDL037C)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CTF4(YPR135W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DEG1(YFL001W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DGR2(YKL121W_p)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIT1(YDR403W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:FAA1(YOR317W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FMP30(YPL103C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FSH3(YOR280C)|FD-Score:4.29|P-value:8.93E-6||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FUN14(YAL008W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Mitochondrial protein of unknown function Gene:GAL10(YBR019C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GIP4(YAL031C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate Gene:IFA38(YBR159W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IMD3(YLR432W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:KAR9(YPL269W)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:MAK32(YCR019W)|FD-Score:-3.9|P-value:4.72E-5||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MRPS9(YBR146W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NIT2(YJL126W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OAC1(YKL120W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OSM1(YJR051W)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PAM17(YKR065C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PEX28(YHR150W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PTP2(YOR208W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RAD33(YML011C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAS2(YNL098C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RCK2(YLR248W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:REC107(YJR021C)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:SAK1(YER129W)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SDH1(YKL148C)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:UGA3(YDL170W)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VMR1(YHL035C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YAL065C(YAL065C_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YDL129W(YDL129W_p)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL108C(YGL108C_p)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YKL222C(YKL222C)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Putative protein of unknown function Gene:BSC1(YDL037C)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CTF4(YPR135W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DEG1(YFL001W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DGR2(YKL121W_p)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIT1(YDR403W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:FAA1(YOR317W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FMP30(YPL103C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FSH3(YOR280C)|FD-Score:4.29|P-value:8.93E-6||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FUN14(YAL008W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Mitochondrial protein of unknown function Gene:GAL10(YBR019C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GIP4(YAL031C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate Gene:IFA38(YBR159W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IMD3(YLR432W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:KAR9(YPL269W)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:MAK32(YCR019W)|FD-Score:-3.9|P-value:4.72E-5||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MRPS9(YBR146W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NIT2(YJL126W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:OAC1(YKL120W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OSM1(YJR051W)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PAM17(YKR065C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PEX28(YHR150W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PTP2(YOR208W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RAD33(YML011C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAS2(YNL098C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RCK2(YLR248W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:REC107(YJR021C)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:SAK1(YER129W)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SDH1(YKL148C)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:UGA3(YDL170W)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VMR1(YHL035C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YAL065C(YAL065C_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YDL129W(YDL129W_p)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL108C(YGL108C_p)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YKL222C(YKL222C)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL150W3.944.00E-50.25RPC53RNA polymerase III subunit C53
YGR095C3.691.11E-40.10RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YPL093W3.601.61E-40.11NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YAL041W3.492.44E-40.07CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YGL055W3.423.16E-40.00OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YLR140W_d3.423.18E-40.15YLR140W_dDubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I
YBR193C3.265.54E-40.18MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YOL026C3.080.001040.04MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YDL004W3.040.001190.17ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YBR121C2.870.002030.01GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YPR016C2.860.002120.09TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YOL144W2.770.002770.01NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YMR113W2.760.002870.00FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YOR310C2.760.002890.02NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YLR129W2.740.003060.02DIP2Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL103C4.602.13E-6FMP30Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D
YPL269W4.503.43E-6KAR9Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
YOR317W4.376.08E-6FAA1Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication
YOR280C4.298.93E-6FSH3Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2
YAL008W4.191.42E-5FUN14Mitochondrial protein of unknown function
YER129W4.131.78E-5SAK1Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
YLR432W4.131.83E-5IMD3Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed
YFL001W4.102.06E-5DEG1tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
YGL108C_p4.012.97E-5YGL108C_pProtein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress
YJR021C3.963.80E-5REC107Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation
YAL065C_p3.551.90E-4YAL065C_pPutative protein of unknown function; has homology to FLO1; possible pseudogene
YAL031C3.354.01E-4GIP4Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate
YLR248W3.334.34E-4RCK2Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
YBR146W3.314.62E-4MRPS9Mitochondrial ribosomal protein of the small subunit
YPR135W3.285.13E-4CTF4Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion

GO enrichment analysis for SGTC_2047
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0781.56E-9SGTC_31449087057 49.5 μMChembridge (Drug-like library)172496180.118644
0.0681.65E-7SGTC_27857756463 72.7 μMChembridge (Drug-like library)22015510.114754
0.0663.72E-7SGTC_31459085516 49.5 μMChembridge (Drug-like library)252361950.122807fatty acid desaturase (OLE1)
0.0622.01E-6SGTC_1646st011709 75.6 μMTimTec (Natural product derivative library)57578430.0714286
0.0587.87E-6SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.0943396RNA processing & uracil transport
0.0588.30E-6SGTC_387norethindrone acetate 66.7 μMMiscellaneous5411970.101449
0.0589.38E-6SGTC_432NS 1619 61.3 μMICCB bioactive library45520.0892857
0.0571.16E-5SGTC_1684st024538 14.6 μMTimTec (Natural product derivative library)67328590.0793651
0.0561.55E-5SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.105263fatty acid desaturase (OLE1)
0.0561.75E-5SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0655738TSC3-RPN4
0.0552.34E-5SGTC_9731325-0077 65.9 μMChemDiv (Drug-like library)28351490.0754717
0.0552.71E-5SGTC_1667st014849 34.6 μMTimTec (Natural product derivative library)53484600.0675676Golgi
0.0542.98E-5SGTC_1659st012954 42.1 μMTimTec (Natural product derivative library)242072620.0540541
0.0525.55E-5SGTC_10174239-0030 168.0 μMChemDiv (Drug-like library)11495930.112903
0.0526.13E-5SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.12963RNA pol III & RNase P/MRP

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20054024620188 μM0.35236743Chembridge (Fragment library)179.215721.66513
SGTC_23157733312200 μM0.348837726316Chembridge (Fragment library)210.25291.93523
SGTC_3232913287449.47 μM0.3255813784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_1765st04481590.4 μM0.304348645695TimTec (Natural product derivative library)221.209341.51715
SGTC_5544483-2211187 μM0.297872747107ChemDiv (Drug-like library)285.29462.45314
SGTC_12550566-0045221 μM0.2978722788469ChemDiv (Drug-like library)304.728323.04223excess fatty acid
SGTC_8080711-0032135 μM0.284252555ChemDiv (Drug-like library)370.218382.92115
SGTC_10563448-1962115 μM0.276596715507ChemDiv (Drug-like library)260.311582.55305amide catabolism
SGTC_2897904859812.99 μM0.274516467404Chembridge (Drug-like library)283.321782.99113
SGTC_12390337-058435.4 μM0.272727914301ChemDiv (Drug-like library)318.077622.18306unfolded protein response