5246248

2-fluoro-N-pyridin-3-ylbenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2048
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 677046
SMILES C1=CC=C(C(=C1)C(=O)NC2=CN=CC=C2)F
Standardized SMILES Fc1ccccc1C(=O)Nc2cccnc2
Molecular weight 216.2111
ALogP 1.67
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.71
% growth inhibition (Hom. pool) 2.34


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 677046
Download HIP data (tab-delimited text)  (excel)
Gene:CDC3(YLR314C)|FD-Score:3.3|P-value:4.76E-4|Clearance:0.16||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DIS3(YOL021C)|FD-Score:-4.64|P-value:1.74E-6|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:DML1(YMR211W)|FD-Score:3.84|P-value:6.17E-5|Clearance:0.28||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ECO1(YFR027W)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.25||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:GRC3(YLL035W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.26||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:NDC1(YML031W)|FD-Score:5.66|P-value:7.69E-9|Clearance:1.07||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NOP56(YLR197W)|FD-Score:-4.82|P-value:7.18E-7|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PKC1(YBL105C)|FD-Score:-3.82|P-value:6.68E-5|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PZF1(YPR186C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RPC53(YDL150W)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPS13(YDR064W)|FD-Score:4.59|P-value:2.27E-6|Clearance:0.75||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:TAM41(YGR046W)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:CDC3(YLR314C)|FD-Score:3.3|P-value:4.76E-4|Clearance:0.16||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DIS3(YOL021C)|FD-Score:-4.64|P-value:1.74E-6|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:DML1(YMR211W)|FD-Score:3.84|P-value:6.17E-5|Clearance:0.28||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ECO1(YFR027W)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.25||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:GRC3(YLL035W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.26||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:NDC1(YML031W)|FD-Score:5.66|P-value:7.69E-9|Clearance:1.07||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NOP56(YLR197W)|FD-Score:-4.82|P-value:7.18E-7|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PKC1(YBL105C)|FD-Score:-3.82|P-value:6.68E-5|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PZF1(YPR186C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RPC53(YDL150W)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPS13(YDR064W)|FD-Score:4.59|P-value:2.27E-6|Clearance:0.75||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:TAM41(YGR046W)|FD-Score:-3.46|P-value:2.70E-4|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 677046
Download HOP data (tab-delimited text)  (excel)
Gene:ADH5(YBR145W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:APL3(YBL037W)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ATP14(YLR295C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BNI4(YNL233W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:CAP1(YKL007W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CWH41(YGL027C)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:CYS3(YAL012W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DOS2(YDR068W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EOS1(YNL080C)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:GCN2(YDR283C)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GRX3(YDR098C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:GTT2(YLL060C)|FD-Score:3.73|P-value:9.52E-5||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HFD1(YMR110C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:IRC21(YMR073C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JID1(YPR061C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:MEI5(YPL121C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p Gene:MGR2(YPL098C)|FD-Score:-4.51|P-value:3.18E-6||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MSC3(YLR219W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:NGR1(YBR212W)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NUP120(YKL057C)|FD-Score:-7.37|P-value:8.37E-14||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUP133(YKR082W)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:PAU5(YFL020C)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PKR1(YMR123W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PNC1(YGL037C)|FD-Score:7.4|P-value:6.87E-14||SGD DESC:Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress Gene:RAM1(YDL090C)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RPL24A(YGL031C)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPS4A(YJR145C)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SHU1(YHL006C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:UBX5(YDR330W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:ULS1(YOR191W)|FD-Score:4.82|P-value:7.16E-7||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YGL039W(YGL039W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:ADH5(YBR145W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:APL3(YBL037W)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ATP14(YLR295C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BNI4(YNL233W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:CAP1(YKL007W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CWH41(YGL027C)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:CYS3(YAL012W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DOS2(YDR068W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EOS1(YNL080C)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:GCN2(YDR283C)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GRX3(YDR098C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:GTT2(YLL060C)|FD-Score:3.73|P-value:9.52E-5||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HFD1(YMR110C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:IRC21(YMR073C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JID1(YPR061C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:MEI5(YPL121C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p Gene:MGR2(YPL098C)|FD-Score:-4.51|P-value:3.18E-6||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MSC3(YLR219W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:NGR1(YBR212W)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NUP120(YKL057C)|FD-Score:-7.37|P-value:8.37E-14||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUP133(YKR082W)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:PAU5(YFL020C)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PKR1(YMR123W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PNC1(YGL037C)|FD-Score:7.4|P-value:6.87E-14||SGD DESC:Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress Gene:RAM1(YDL090C)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RPL24A(YGL031C)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPS4A(YJR145C)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SHU1(YHL006C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:UBX5(YDR330W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:ULS1(YOR191W)|FD-Score:4.82|P-value:7.16E-7||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YGL039W(YGL039W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YML031W5.667.69E-91.07NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YDR064W4.592.27E-60.75RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YMR211W3.846.17E-50.28DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YLL035W3.561.84E-40.26GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
YLR314C3.304.76E-40.16CDC3Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YFR027W3.158.21E-40.25ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YPL117C2.890.001900.04IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
YAL034C-B_d2.860.002150.08YAL034C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR398W2.770.002800.03UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YIL019W2.740.003110.00FAF1Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
YLR223C2.730.003130.07IFH1Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
YGR267C2.670.003840.13FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YHR190W2.530.005630.02ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YDR167W2.510.006010.01TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YGL155W2.500.006150.02CDC43Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL037C7.406.87E-14PNC1Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
YOR191W4.827.16E-7ULS1Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
YBR212W4.513.28E-6NGR1RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase
YGL031C4.121.92E-5RPL24ARibosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication
YIL018W3.983.44E-5RPL2BRibosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures
YBL037W3.953.84E-5APL3Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport
YLR110C3.749.07E-5CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YLL060C3.739.52E-5GTT2Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
YDL216C3.611.54E-4RRI1Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling
YBR145W3.541.97E-4ADH5Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
YGL039W3.532.10E-4YGL039WOxidoreductase shown to reduce carbonyl compounds to chiral alcohols
YPL121C3.522.16E-4MEI5Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p
YHL006C3.512.21E-4SHU1Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
YDR330W3.492.38E-4UBX5UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p
YDR098C3.472.59E-4GRX3Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36

GO enrichment analysis for SGTC_2048
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1301.04E-23SGTC_20455106652 78.0 μMChembridge (Fragment library)6733490.140351
0.1102.57E-17SGTC_31259127534 49.5 μMChembridge (Drug-like library)16392350.133333
0.0983.49E-14SGTC_28769059903 26.0 μMChembridge (Drug-like library)69160950.145161
0.0823.39E-10SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.117647DNA damage response
0.0707.49E-8SGTC_1524469-0003 551.2 μMChemDiv (Drug-like library)6972800.142857
0.0708.11E-8SGTC_6033794-0023 45.9 μMChemDiv (Drug-like library)38022600.0615385
0.0682.08E-7SGTC_20305175110 200.0 μMChembridge (Fragment library)28319860.150943Golgi
0.0655.07E-7SGTC_2385hr heat shock (37°C) + nsc-638432 8.9 μMMiscellaneous3680900.0833333heat shock/prefoldin
0.0603.67E-6SGTC_2042p-Dimethylaminoacetophenone 200.0 μMChembridge (Fragment library)750370.130435
0.0594.90E-6SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)31320570.0821918DNA damage response
0.0588.47E-6SGTC_1520flindersine 88.0 μMTimTec (Pure natural product library)682300.1
0.0571.05E-5SGTC_28237987624 58.4 μMChembridge (Drug-like library)22394400.137931
0.0571.22E-5SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.09375
0.0561.65E-5SGTC_12450418-0076 17.2 μMChemDiv (Drug-like library)67901140.0540541ERG2
0.0552.59E-5SGTC_32789139605 49.5 μMChembridge (Drug-like library)79588590.354167

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21245246320200 μM0.694444579342Chembridge (Fragment library)216.2110631.66913heme biosynthesis & mitochondrial translocase
SGTC_2979901255245.46 μM0.58139517100905Chembridge (Drug-like library)282.724343.03612
SGTC_22757947463200 μM0.5454551225414Chembridge (Fragment library)216.235880.85813iron homeostasis
SGTC_8480368-008985.6 μM0.5762535ChemDiv (Drug-like library)267.110722.79212iron homeostasis
SGTC_22897946404165.07 μM0.4634151246120Chembridge (Fragment library)234.2015261.87514
SGTC_2969909140828.7 μM0.4583334286311Chembridge (Drug-like library)254.326923.31812
SGTC_23246149169200 μM0.4285712888231Chembridge (Fragment library)247.273062.0124
SGTC_3082911700849.47 μM0.41666741448529Chembridge (Drug-like library)240.300342.44112
SGTC_2968909066333.37 μM0.41509425236811Chembridge (Drug-like library)325.224123.43413
SGTC_21876125751200 μM0.404255874498Chembridge (Fragment library)235.1895861.15215RSC complex & mRNA processing