5262831

2-[4-[(3-fluorophenyl)methyl]piperazin-1-yl]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2049
Screen concentration 67.2 μM
Source Chembridge (Fragment library)
PubChem CID 763417
SMILES C1CN(CCN1CC2=CC(=CC=C2)F)C3=NC=CC=N3
Standardized SMILES Fc1cccc(CN2CCN(CC2)c3ncccn3)c1
Molecular weight 272.3207
ALogP 2.48
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.58
% growth inhibition (Hom. pool) 8.98


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 763417
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.84|P-value:6.14E-5|Clearance:0.15||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ALG13(YGL047W)|FD-Score:3.3|P-value:4.81E-4|Clearance:0.04||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ASA1(YPR085C)|FD-Score:3.86|P-value:5.77E-5|Clearance:0.01||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CCA1(YER168C)|FD-Score:3.89|P-value:5.05E-5|Clearance:0.03||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCT2(YIL142W)|FD-Score:-6.29|P-value:1.57E-10|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC39(YCR093W)|FD-Score:3.26|P-value:5.50E-4|Clearance:0.17||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DIM1(YPL266W)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:ESA1(YOR244W)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.27||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:GPI8(YDR331W)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.03||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HSH49(YOR319W)|FD-Score:-4.04|P-value:2.65E-5|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HYP2(YEL034W)|FD-Score:-7.44|P-value:5.06E-14|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IRA1(YBR140C)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.01||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KRE33(YNL132W)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.03||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MSL5(YLR116W)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.03||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:MTR4(YJL050W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.08||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NFS1(YCL017C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.03||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NOP8(YOL144W)|FD-Score:-3.12|P-value:9.01E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP159(YIL115C)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.09||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP49(YGL172W)|FD-Score:-3.75|P-value:9.01E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:OLE1(YGL055W)|FD-Score:3.89|P-value:4.96E-5|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:POP7(YBR167C)|FD-Score:4.47|P-value:3.97E-6|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RHO1(YPR165W)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.03||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL10(YLR075W)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:SPN1(YPR133C)|FD-Score:-9.89|P-value:2.34E-23|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPP382(YLR424W)|FD-Score:4.85|P-value:6.13E-7|Clearance:0.38||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:TFC3(YAL001C)|FD-Score:6.66|P-value:1.38E-11|Clearance:1.81||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YDR396W(YDR396W_d)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YEF3(YLR249W)|FD-Score:-3.87|P-value:5.48E-5|Clearance:0||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YGR073C(YGR073C_d)|FD-Score:4.37|P-value:6.11E-6|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:ACC1(YNR016C)|FD-Score:3.84|P-value:6.14E-5|Clearance:0.15||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ALG13(YGL047W)|FD-Score:3.3|P-value:4.81E-4|Clearance:0.04||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ASA1(YPR085C)|FD-Score:3.86|P-value:5.77E-5|Clearance:0.01||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CCA1(YER168C)|FD-Score:3.89|P-value:5.05E-5|Clearance:0.03||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCT2(YIL142W)|FD-Score:-6.29|P-value:1.57E-10|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-3.65|P-value:1.33E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC39(YCR093W)|FD-Score:3.26|P-value:5.50E-4|Clearance:0.17||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DIM1(YPL266W)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:ESA1(YOR244W)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.27||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:GPI8(YDR331W)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.03||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HSH49(YOR319W)|FD-Score:-4.04|P-value:2.65E-5|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HYP2(YEL034W)|FD-Score:-7.44|P-value:5.06E-14|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IRA1(YBR140C)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.01||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KRE33(YNL132W)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.03||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MSL5(YLR116W)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.03||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:MTR4(YJL050W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.08||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NFS1(YCL017C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.03||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NOP8(YOL144W)|FD-Score:-3.12|P-value:9.01E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP159(YIL115C)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.09||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP49(YGL172W)|FD-Score:-3.75|P-value:9.01E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:OLE1(YGL055W)|FD-Score:3.89|P-value:4.96E-5|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:POP7(YBR167C)|FD-Score:4.47|P-value:3.97E-6|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RHO1(YPR165W)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.03||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL10(YLR075W)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:SPN1(YPR133C)|FD-Score:-9.89|P-value:2.34E-23|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPP382(YLR424W)|FD-Score:4.85|P-value:6.13E-7|Clearance:0.38||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:TFC3(YAL001C)|FD-Score:6.66|P-value:1.38E-11|Clearance:1.81||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YDR396W(YDR396W_d)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YEF3(YLR249W)|FD-Score:-3.87|P-value:5.48E-5|Clearance:0||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YGR073C(YGR073C_d)|FD-Score:4.37|P-value:6.11E-6|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 763417
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADY2(YCR010C)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AIM43(YPL099C)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD4(YOR374W)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:ALG5(YPL227C)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APE3(YBR286W)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ARG8(YOL140W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ATS1(YAL020C)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:AVT4(YNL101W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BEM2(YER155C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BRE2(YLR015W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BTS1(YPL069C)|FD-Score:-3.8|P-value:7.15E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF4(YKR036C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CBC2(YPL178W)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CLB2(YPR119W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG6(YNL041C)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPD1(YGR247W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CWC15(YDR163W)|FD-Score:4.79|P-value:8.29E-7||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DAL1(YIR027C)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DCV1(YFR012W)|FD-Score:3.27|P-value:5.28E-4||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DFG16(YOR030W)|FD-Score:-4.64|P-value:1.76E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DUF1(YOL087C)|FD-Score:4.33|P-value:7.30E-6||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM8(YBR076W)|FD-Score:7.16|P-value:4.17E-13||SGD DESC:Non-essential protein of unknown function Gene:ENV10(YLR065C)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FAR11(YNL127W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FMP23(YBR047W_p)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRT2(YAL028W)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:FUR4(YBR021W)|FD-Score:3.82|P-value:6.71E-5||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN20(YFR009W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GET3(YDL100C)|FD-Score:3.09|P-value:0.00100||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GGA1(YDR358W)|FD-Score:-5.75|P-value:4.34E-9||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GIN4(YDR507C)|FD-Score:-7.1|P-value:6.31E-13||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:6.07|P-value:6.41E-10||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPA2(YER020W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GYP6(YJL044C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HFA1(YMR207C)|FD-Score:-3.15|P-value:8.29E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HIM1(YDR317W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:IGD1(YFR017C)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:ISY1(YJR050W)|FD-Score:-10.5|P-value:4.45E-26||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:JEM1(YJL073W)|FD-Score:6.55|P-value:2.79E-11||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KIN4(YOR233W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LSM7(YNL147W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDL2(YPL270W)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MPD2(YOL088C)|FD-Score:5.68|P-value:6.79E-9||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPS16(YPL013C)|FD-Score:-5.42|P-value:3.04E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NUP170(YBL079W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PCD1(YLR151C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PDC6(YGR087C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PDR16(YNL231C)|FD-Score:-5.86|P-value:2.34E-9||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PHO85(YPL031C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PIB1(YDR313C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PMP3(YDR276C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRO2(YOR323C)|FD-Score:6.24|P-value:2.26E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTH1(YHR189W)|FD-Score:-6.24|P-value:2.19E-10||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:PTH2(YBL057C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PTK2(YJR059W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAD51(YER095W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RCR2(YDR003W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL26A(YLR344W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RSM23(YGL129C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:SAC1(YKL212W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP190(YKR028W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SCH9(YHR205W)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SCO2(YBR024W)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SET5(YHR207C)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SGT2(YOR007C)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SKG3(YLR187W_p)|FD-Score:4.33|P-value:7.36E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLT2(YHR030C)|FD-Score:7.22|P-value:2.68E-13||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMA1(YPL027W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNT309(YPR101W)|FD-Score:-3.74|P-value:9.14E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SRL1(YOR247W)|FD-Score:4.94|P-value:3.83E-7||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SSA4(YER103W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SWC3(YAL011W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TFB6(YOR352W)|FD-Score:4.32|P-value:7.77E-6||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:THI6(YPL214C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TMA22(YJR014W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress Gene:TRP5(YGL026C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP23(YDR084C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VPS1(YKR001C)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS53(YJL029C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:YDR379C-A(YDR379C-A)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YEL1(YBL060W)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER188W(YER188W_d)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGR228W(YGR228W_d)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIA6(YIL006W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL168W(YIL168W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJL135W(YJL135W_d)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YKL075C(YKL075C_p)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YLR012C(YLR012C_p)|FD-Score:-4.53|P-value:2.97E-6||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR125W(YLR125W_p)|FD-Score:5.21|P-value:9.68E-8||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR236C(YLR236C_d)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YND1(YER005W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL010W(YNL010W_p)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL034W(YNL034W_p)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YNL057W(YNL057W_d)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR065C(YNR065C_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR318C(YOR318C_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR376W(YOR376W_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR089W(YPR089W_p)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR099C(YPR099C_d)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPR123C(YPR123C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ADE4(YMR300C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADY2(YCR010C)|FD-Score:3.95|P-value:3.87E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AIM43(YPL099C)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD4(YOR374W)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:ALG5(YPL227C)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APE3(YBR286W)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ARG8(YOL140W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ATS1(YAL020C)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:AVT4(YNL101W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BEM2(YER155C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BRE2(YLR015W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BTS1(YPL069C)|FD-Score:-3.8|P-value:7.15E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF4(YKR036C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CBC2(YPL178W)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CLB2(YPR119W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG6(YNL041C)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPD1(YGR247W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CWC15(YDR163W)|FD-Score:4.79|P-value:8.29E-7||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DAL1(YIR027C)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DCV1(YFR012W)|FD-Score:3.27|P-value:5.28E-4||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DFG16(YOR030W)|FD-Score:-4.64|P-value:1.76E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DUF1(YOL087C)|FD-Score:4.33|P-value:7.30E-6||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM8(YBR076W)|FD-Score:7.16|P-value:4.17E-13||SGD DESC:Non-essential protein of unknown function Gene:ENV10(YLR065C)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FAR11(YNL127W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FMP23(YBR047W_p)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRT2(YAL028W)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:FUR4(YBR021W)|FD-Score:3.82|P-value:6.71E-5||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN20(YFR009W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GET3(YDL100C)|FD-Score:3.09|P-value:0.00100||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GGA1(YDR358W)|FD-Score:-5.75|P-value:4.34E-9||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GIN4(YDR507C)|FD-Score:-7.1|P-value:6.31E-13||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:6.07|P-value:6.41E-10||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPA2(YER020W)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GYP6(YJL044C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HFA1(YMR207C)|FD-Score:-3.15|P-value:8.29E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HIM1(YDR317W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:IGD1(YFR017C)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:ISY1(YJR050W)|FD-Score:-10.5|P-value:4.45E-26||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:JEM1(YJL073W)|FD-Score:6.55|P-value:2.79E-11||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KIN4(YOR233W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LSM7(YNL147W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDL2(YPL270W)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MPD2(YOL088C)|FD-Score:5.68|P-value:6.79E-9||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPS16(YPL013C)|FD-Score:-5.42|P-value:3.04E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NUP170(YBL079W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PCD1(YLR151C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PDC6(YGR087C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PDR16(YNL231C)|FD-Score:-5.86|P-value:2.34E-9||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PHO85(YPL031C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PIB1(YDR313C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PMP3(YDR276C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRO2(YOR323C)|FD-Score:6.24|P-value:2.26E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTH1(YHR189W)|FD-Score:-6.24|P-value:2.19E-10||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:PTH2(YBL057C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PTK2(YJR059W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAD51(YER095W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RCR2(YDR003W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL26A(YLR344W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RSM23(YGL129C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:SAC1(YKL212W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP190(YKR028W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SCH9(YHR205W)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SCO2(YBR024W)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SET5(YHR207C)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SGT2(YOR007C)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SKG3(YLR187W_p)|FD-Score:4.33|P-value:7.36E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLT2(YHR030C)|FD-Score:7.22|P-value:2.68E-13||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMA1(YPL027W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNT309(YPR101W)|FD-Score:-3.74|P-value:9.14E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SRL1(YOR247W)|FD-Score:4.94|P-value:3.83E-7||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SSA4(YER103W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SWC3(YAL011W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TFB6(YOR352W)|FD-Score:4.32|P-value:7.77E-6||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:THI6(YPL214C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TMA22(YJR014W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress Gene:TRP5(YGL026C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP23(YDR084C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VPS1(YKR001C)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS53(YJL029C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Gene:YDR379C-A(YDR379C-A)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YEL1(YBL060W)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER188W(YER188W_d)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGR228W(YGR228W_d)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIA6(YIL006W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL168W(YIL168W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJL135W(YJL135W_d)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YKL075C(YKL075C_p)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YLR012C(YLR012C_p)|FD-Score:-4.53|P-value:2.97E-6||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR125W(YLR125W_p)|FD-Score:5.21|P-value:9.68E-8||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR236C(YLR236C_d)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YND1(YER005W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL010W(YNL010W_p)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL034W(YNL034W_p)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YNL057W(YNL057W_d)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR065C(YNR065C_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR318C(YOR318C_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR376W(YOR376W_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR089W(YPR089W_p)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR099C(YPR099C_d)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPR123C(YPR123C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YAL001C6.661.38E-111.81TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YLR424W4.856.13E-70.38SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YBR167C4.473.97E-60.09POP7Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR073C_d4.376.11E-60.21YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YOR244W4.171.54E-50.27ESA1Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy
YGL055W3.894.96E-50.00OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YER168C3.895.05E-50.03CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YPR085C3.865.77E-50.01ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YNR016C3.846.14E-50.15ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YCL017C3.691.14E-40.03NFS1Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
YDR396W_d3.661.27E-40.08YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YJL050W3.571.76E-40.08MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YBR140C3.502.33E-40.01IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YIL115C3.492.42E-40.09NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YDR331W3.393.44E-40.03GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR030C7.222.68E-13SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YBR076W7.164.17E-13ECM8Non-essential protein of unknown function
YJL073W6.552.79E-11JEM1DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
YOR323C6.242.26E-10PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YMR311C6.076.41E-10GLC8Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
YOL088C5.686.79E-9MPD2Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
YBR047W_p5.501.89E-8FMP23_pPutative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YLR125W_p5.219.68E-8YLR125W_pPutative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene
YOR374W5.101.71E-7ALD4Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed
YPR089W_p5.071.96E-7YPR089W_pProtein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p
YOR007C5.032.46E-7SGT2Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
YPL270W4.973.33E-7MDL2Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
YOR247W4.943.83E-7SRL1Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication
YDR163W4.798.29E-7CWC15Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p
YOR233W4.711.25E-6KIN4Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2049
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1233.14E-21SGTC_22867945697 125.0 μMChembridge (Fragment library)12457330.225806
0.1057.47E-16SGTC_29249010168 62.8 μMChembridge (Drug-like library)29939290.15625cell wall signaling
0.0992.96E-14SGTC_500h-89 112.0 μMICCB bioactive library4492410.103896cell wall signaling
0.0939.42E-13SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.107143cell wall signaling
0.0921.43E-12SGTC_1614st002383 48.6 μMTimTec (Natural product derivative library)19472350.101266cell wall signaling
0.0896.04E-12SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.115385cell wall signaling
0.0897.50E-12SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.0735294cell wall
0.0872.41E-11SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0595238cell wall
0.0864.21E-11SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.105263cell wall
0.0864.27E-11SGTC_397arbidol 50.0 μMMiscellaneous1314100.0697674cell wall
0.0841.11E-10SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0694444
0.0814.93E-10SGTC_8830850-0245 24.6 μMChemDiv (Drug-like library)68232290.0933333cell wall
0.0815.09E-10SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.169492cell wall signaling
0.0791.05E-9SGTC_8630466-0191 129.0 μMChemDiv (Drug-like library)7651600.09375cell wall
0.0781.71E-9SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.0909091cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5733702-055384.7 μM0.4390241222756ChemDiv (Drug-like library)424.17284.94102
SGTC_3140909424349.47 μM0.42857119291112Chembridge (Drug-like library)342.4072233.32204
SGTC_7294092-058594.1 μM0.4035092169061ChemDiv (Drug-like library)390.4500234.5504
SGTC_5154092-1064102 μM0.4717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_1784092-080687.51 μM0.3752169044ChemDiv (Drug-like library)328.87895.05102ergosterol depletion effects on membrane
SGTC_1734st03716869.4 μM0.338983708907TimTec (Natural product derivative library)288.2770231.68904
SGTC_20505263056171 μM0.333333763468Chembridge (Fragment library)268.35353.29113fatty acid desaturase (OLE1)
SGTC_2880904298358.44 μM0.3220346464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)
SGTC_1818st05213656.3 μM0.321429674169TimTec (Natural product derivative library)339.861784.40212NEO1-PIK1
SGTC_13963511-000798.6 μM0.318182X1396ChemDiv (Drug-like library)421.843265.08441mitochondrial processes