5263056

4-[(4-phenylpiperazin-1-yl)methyl]phenol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2050
Screen concentration 171.0 μM
Source Chembridge (Fragment library)
PubChem CID 763468
SMILES C1CN(CCN1CC2=CC=C(C=C2)O)C3=CC=CC=C3
Standardized SMILES Oc1ccc(CN2CCN(CC2)c3ccccc3)cc1
Molecular weight 268.3535
ALogP 3.29
H-bond donor count 1
H-bond acceptor count 3
Response signature fatty acid desaturase (OLE1)

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.82
% growth inhibition (Hom. pool) 8.98


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 763468
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:4.08|P-value:2.20E-5|Clearance:0.08||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:4.57|P-value:2.45E-6|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:6.81|P-value:4.90E-12|Clearance:0.33||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:COG4(YPR105C)|FD-Score:4.11|P-value:1.97E-5|Clearance:0.03||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTF13(YMR094W)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.03||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:ERG11(YHR007C)|FD-Score:8.88|P-value:3.31E-19|Clearance:0.5||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG26(YGL001C)|FD-Score:4.68|P-value:1.43E-6|Clearance:0.07||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FAS1(YKL182W)|FD-Score:-6.82|P-value:4.69E-12|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GPI8(YDR331W)|FD-Score:13|P-value:9.64E-39|Clearance:3.07||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HSH49(YOR319W)|FD-Score:5.79|P-value:3.46E-9|Clearance:0.01||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRA1(YBR140C)|FD-Score:9.9|P-value:2.12E-23|Clearance:1.02||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MCM7(YBR202W)|FD-Score:-3.23|P-value:6.26E-4|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MGE1(YOR232W)|FD-Score:7.49|P-value:3.47E-14|Clearance:0.68||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NAF1(YNL124W)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.04||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NFS1(YCL017C)|FD-Score:-4.53|P-value:2.90E-6|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NMD3(YHR170W)|FD-Score:3.79|P-value:7.65E-5|Clearance:0.04||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG1(YPL093W)|FD-Score:4.94|P-value:3.93E-7|Clearance:0.26||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP8(YOL144W)|FD-Score:4.3|P-value:8.50E-6|Clearance:0.08||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUF2(YOL069W)|FD-Score:3.34|P-value:4.16E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:OLE1(YGL055W)|FD-Score:22.6|P-value:4.13E-113|Clearance:9.61||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:4.33|P-value:7.52E-6|Clearance:0.03||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POP5(YAL033W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.11||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE2(YPR103W)|FD-Score:-4.1|P-value:2.08E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PUP1(YOR157C)|FD-Score:3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFC5(YBR087W)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.11||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RLP24(YLR009W)|FD-Score:4|P-value:3.16E-5|Clearance:0.07||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC82(YPR190C)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPL10(YLR075W)|FD-Score:8.34|P-value:3.56E-17|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL3(YOR063W)|FD-Score:3.12|P-value:9.02E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RPL33A(YPL143W)|FD-Score:4.61|P-value:2.05E-6|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:5.78|P-value:3.70E-9|Clearance:0.84||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEC21(YNL287W)|FD-Score:3.09|P-value:9.89E-4|Clearance:0.03||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC23(YPR181C)|FD-Score:3.2|P-value:6.91E-4|Clearance:0.06||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC65(YML105C)|FD-Score:6.48|P-value:4.65E-11|Clearance:0.52||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SGT1(YOR057W)|FD-Score:-5.64|P-value:8.31E-9|Clearance:0||SGD DESC:Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress Gene:SUI2(YJR007W)|FD-Score:3.89|P-value:4.93E-5|Clearance:0.03||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUP45(YBR143C)|FD-Score:3.37|P-value:3.79E-4|Clearance:0.03||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TFC8(YPL007C)|FD-Score:-4.14|P-value:1.77E-5|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:TIF11(YMR260C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.02||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIF35(YDR429C)|FD-Score:4.56|P-value:2.59E-6|Clearance:0.23||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIF6(YPR016C)|FD-Score:3.93|P-value:4.22E-5|Clearance:0.04||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TIM17(YJL143W)|FD-Score:8.33|P-value:4.14E-17|Clearance:0.69||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM40(YMR203W)|FD-Score:5.96|P-value:1.29E-9|Clearance:0.16||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSC13(YDL015C)|FD-Score:-3.25|P-value:5.72E-4|Clearance:0||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:YBR190W(YBR190W_d)|FD-Score:7.64|P-value:1.09E-14|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:8.38|P-value:2.63E-17|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:3.75|P-value:8.93E-5|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:3.86|P-value:5.63E-5|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.56|P-value:2.57E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPL142C(YPL142C_d)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:CCT2(YIL142W)|FD-Score:4.08|P-value:2.20E-5|Clearance:0.08||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:4.57|P-value:2.45E-6|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:6.81|P-value:4.90E-12|Clearance:0.33||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:COG4(YPR105C)|FD-Score:4.11|P-value:1.97E-5|Clearance:0.03||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTF13(YMR094W)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.03||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:ERG11(YHR007C)|FD-Score:8.88|P-value:3.31E-19|Clearance:0.5||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG26(YGL001C)|FD-Score:4.68|P-value:1.43E-6|Clearance:0.07||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FAS1(YKL182W)|FD-Score:-6.82|P-value:4.69E-12|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GPI8(YDR331W)|FD-Score:13|P-value:9.64E-39|Clearance:3.07||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:HSH49(YOR319W)|FD-Score:5.79|P-value:3.46E-9|Clearance:0.01||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRA1(YBR140C)|FD-Score:9.9|P-value:2.12E-23|Clearance:1.02||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MCM7(YBR202W)|FD-Score:-3.23|P-value:6.26E-4|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MGE1(YOR232W)|FD-Score:7.49|P-value:3.47E-14|Clearance:0.68||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NAF1(YNL124W)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.04||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NFS1(YCL017C)|FD-Score:-4.53|P-value:2.90E-6|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NMD3(YHR170W)|FD-Score:3.79|P-value:7.65E-5|Clearance:0.04||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG1(YPL093W)|FD-Score:4.94|P-value:3.93E-7|Clearance:0.26||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP8(YOL144W)|FD-Score:4.3|P-value:8.50E-6|Clearance:0.08||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUF2(YOL069W)|FD-Score:3.34|P-value:4.16E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:OLE1(YGL055W)|FD-Score:22.6|P-value:4.13E-113|Clearance:9.61||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:4.33|P-value:7.52E-6|Clearance:0.03||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POP5(YAL033W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.11||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE2(YPR103W)|FD-Score:-4.1|P-value:2.08E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PUP1(YOR157C)|FD-Score:3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFC5(YBR087W)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.11||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RLP24(YLR009W)|FD-Score:4|P-value:3.16E-5|Clearance:0.07||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC82(YPR190C)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPL10(YLR075W)|FD-Score:8.34|P-value:3.56E-17|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL3(YOR063W)|FD-Score:3.12|P-value:9.02E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RPL33A(YPL143W)|FD-Score:4.61|P-value:2.05E-6|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:5.78|P-value:3.70E-9|Clearance:0.84||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEC21(YNL287W)|FD-Score:3.09|P-value:9.89E-4|Clearance:0.03||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC23(YPR181C)|FD-Score:3.2|P-value:6.91E-4|Clearance:0.06||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC65(YML105C)|FD-Score:6.48|P-value:4.65E-11|Clearance:0.52||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SGT1(YOR057W)|FD-Score:-5.64|P-value:8.31E-9|Clearance:0||SGD DESC:Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress Gene:SUI2(YJR007W)|FD-Score:3.89|P-value:4.93E-5|Clearance:0.03||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUP45(YBR143C)|FD-Score:3.37|P-value:3.79E-4|Clearance:0.03||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TFC8(YPL007C)|FD-Score:-4.14|P-value:1.77E-5|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:TIF11(YMR260C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.02||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIF35(YDR429C)|FD-Score:4.56|P-value:2.59E-6|Clearance:0.23||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIF6(YPR016C)|FD-Score:3.93|P-value:4.22E-5|Clearance:0.04||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TIM17(YJL143W)|FD-Score:8.33|P-value:4.14E-17|Clearance:0.69||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM40(YMR203W)|FD-Score:5.96|P-value:1.29E-9|Clearance:0.16||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSC13(YDL015C)|FD-Score:-3.25|P-value:5.72E-4|Clearance:0||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:YBR190W(YBR190W_d)|FD-Score:7.64|P-value:1.09E-14|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:8.38|P-value:2.63E-17|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:3.75|P-value:8.93E-5|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:3.86|P-value:5.63E-5|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.56|P-value:2.57E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPL142C(YPL142C_d)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 763468
Download HOP data (tab-delimited text)  (excel)
Gene:AGC1(YPR021C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AIM1(YAL046C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM14(YGL160W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ARO1(YDR127W)|FD-Score:8.29|P-value:5.70E-17||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BAS1(YKR099W)|FD-Score:5.36|P-value:4.27E-8||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:CAF16(YFL028C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CBF1(YJR060W)|FD-Score:5.3|P-value:5.65E-8||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:5.57|P-value:1.30E-8||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS5(YLR330W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CLB2(YPR119W)|FD-Score:-4.31|P-value:8.30E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG6(YNL041C)|FD-Score:-6.79|P-value:5.78E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM4(YPL200W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CSR1(YLR380W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DIE2(YGR227W)|FD-Score:5.43|P-value:2.90E-8||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ECM2(YBR065C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ECM4(YKR076W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELA1(YNL230C)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:EMP65(YER140W_p)|FD-Score:5.26|P-value:7.10E-8||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERV29(YGR284C)|FD-Score:6.74|P-value:7.91E-12||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FEN2(YCR028C)|FD-Score:-3.75|P-value:8.68E-5||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FRA2(YGL220W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GCN2(YDR283C)|FD-Score:6.48|P-value:4.47E-11||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:7.17|P-value:3.86E-13||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCS1(YDL226C)|FD-Score:26.5|P-value:1.70E-154||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDT1(YBR187W_p)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GET2(YER083C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:4.96|P-value:3.45E-7||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GPA2(YER020W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GTT1(YIR038C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:HLJ1(YMR161W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HOM3(YER052C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HPM1(YIL110W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HSE1(YHL002W)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HSP31(YDR533C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:IST3(YIR005W)|FD-Score:5.22|P-value:9.02E-8||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:ISY1(YJR050W)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:IVY1(YDR229W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:IZH2(YOL002C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KEL1(YHR158C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LHP1(YDL051W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen Gene:LYP1(YNL268W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MAF1(YDR005C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MDL2(YPL270W)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MEP2(YNL142W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MLP1(YKR095W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MMS1(YPR164W)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MPD1(YOR288C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRPL4(YLR439W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MST1(YKL194C)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC7(YEL033W_p)|FD-Score:5.45|P-value:2.49E-8||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NUP42(YDR192C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:ORM2(YLR350W)|FD-Score:6.09|P-value:5.78E-10||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OST3(YOR085W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PAC10(YGR078C)|FD-Score:-4.86|P-value:5.80E-7||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PBP2(YBR233W)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PBS2(YJL128C)|FD-Score:5.16|P-value:1.20E-7||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PDR11(YIL013C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PMP1(YCR024C-A)|FD-Score:5.47|P-value:2.22E-8||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:POC4(YPL144W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:POP2(YNR052C)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PTC4(YBR125C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:PUF6(YDR496C)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:PUN1(YLR414C)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:RAD4(YER162C)|FD-Score:5.34|P-value:4.60E-8||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RCY1(YJL204C)|FD-Score:20|P-value:4.44E-89||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:-5.24|P-value:7.94E-8||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4|P-value:3.13E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM101(YHL027W)|FD-Score:5.45|P-value:2.54E-8||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RLF2(YPR018W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:ROM2(YLR371W)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL31B(YLR406C)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPL41A(YDL184C)|FD-Score:-3.77|P-value:8.11E-5||SGD DESC:Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication Gene:SCP1(YOR367W)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLT2(YHR030C)|FD-Score:10.5|P-value:5.59E-26||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMA1(YPL027W)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNF4(YGL115W)|FD-Score:-4.72|P-value:1.20E-6||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SPT8(YLR055C)|FD-Score:-4.03|P-value:2.73E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB8(YCR081W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STB5(YHR178W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:STP22(YCL008C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWA2(YDR320C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TOM70(YNL121C)|FD-Score:-3.83|P-value:6.29E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:3.83|P-value:6.54E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.76|P-value:8.55E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.8|P-value:3.24E-9||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP18(YMR071C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMA7(YGR020C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS45(YGL095C)|FD-Score:-3.91|P-value:4.68E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS60(YDR486C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS61(YDR136C_d)|FD-Score:-4.04|P-value:2.67E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAR028W(YAR028W_p)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBR063C(YBR063C_p)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YBR184W(YBR184W_p)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:5.54|P-value:1.48E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEA6(YEL006W)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YET2(YMR040W)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGL260W(YGL260W_p)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR146C-A(YGR146C-A_p)|FD-Score:4.93|P-value:4.10E-7||SGD DESC:Putative protein of unknown function Gene:YGR204C-A(YGR204C-A_p)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR273C(YGR273C_p)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR095W(YHR095W_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:6.75|P-value:7.50E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:-3.9|P-value:4.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKR045C(YKR045C_p)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR036C(YLR036C_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR202C(YLR202C_d)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR349W(YLR349W_d)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YML007C-A(YML007C-A_p)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML054C-A(YML054C-A_p)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Putative protein of unknown function Gene:YMR085W(YMR085W_p)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR122C(YMR122C_d)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL057W(YNL057W_d)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:4.38|P-value:5.83E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YNL115C(YNL115C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YNL143C(YNL143C_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL205C(YNL205C_d)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR065C(YNR065C_p)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR186W(YOR186W_p)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR225W(YOR225W_d)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:7.05|P-value:8.71E-13||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT35(YHR105W)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT52(YKR014C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YPT6(YLR262C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC83(YHR017W)|FD-Score:-4.31|P-value:7.98E-6||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:AGC1(YPR021C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AIM1(YAL046C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM14(YGL160W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ARO1(YDR127W)|FD-Score:8.29|P-value:5.70E-17||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BAS1(YKR099W)|FD-Score:5.36|P-value:4.27E-8||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:CAF16(YFL028C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CBF1(YJR060W)|FD-Score:5.3|P-value:5.65E-8||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:5.57|P-value:1.30E-8||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS5(YLR330W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CLB2(YPR119W)|FD-Score:-4.31|P-value:8.30E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG6(YNL041C)|FD-Score:-6.79|P-value:5.78E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM4(YPL200W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CSR1(YLR380W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DIE2(YGR227W)|FD-Score:5.43|P-value:2.90E-8||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ECM2(YBR065C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ECM4(YKR076W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELA1(YNL230C)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:EMP65(YER140W_p)|FD-Score:5.26|P-value:7.10E-8||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERV29(YGR284C)|FD-Score:6.74|P-value:7.91E-12||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FEN2(YCR028C)|FD-Score:-3.75|P-value:8.68E-5||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FRA2(YGL220W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GCN2(YDR283C)|FD-Score:6.48|P-value:4.47E-11||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:7.17|P-value:3.86E-13||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCS1(YDL226C)|FD-Score:26.5|P-value:1.70E-154||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDT1(YBR187W_p)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GET2(YER083C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:4.96|P-value:3.45E-7||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GPA2(YER020W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GTT1(YIR038C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:HLJ1(YMR161W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HOM3(YER052C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HPM1(YIL110W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HSE1(YHL002W)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HSP31(YDR533C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:IST3(YIR005W)|FD-Score:5.22|P-value:9.02E-8||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:ISY1(YJR050W)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:IVY1(YDR229W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase Gene:IZH2(YOL002C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KEL1(YHR158C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LHP1(YDL051W)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen Gene:LYP1(YNL268W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MAF1(YDR005C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MDL2(YPL270W)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MEP2(YNL142W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MLP1(YKR095W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MMS1(YPR164W)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MPD1(YOR288C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRPL4(YLR439W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MST1(YKL194C)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC7(YEL033W_p)|FD-Score:5.45|P-value:2.49E-8||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NUP42(YDR192C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:ORM2(YLR350W)|FD-Score:6.09|P-value:5.78E-10||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OST3(YOR085W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PAC10(YGR078C)|FD-Score:-4.86|P-value:5.80E-7||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PBP2(YBR233W)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PBS2(YJL128C)|FD-Score:5.16|P-value:1.20E-7||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PDR11(YIL013C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PMP1(YCR024C-A)|FD-Score:5.47|P-value:2.22E-8||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:POC4(YPL144W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:POP2(YNR052C)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PTC4(YBR125C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:PUF6(YDR496C)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:PUN1(YLR414C)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:RAD4(YER162C)|FD-Score:5.34|P-value:4.60E-8||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RCY1(YJL204C)|FD-Score:20|P-value:4.44E-89||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:-5.24|P-value:7.94E-8||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4|P-value:3.13E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM101(YHL027W)|FD-Score:5.45|P-value:2.54E-8||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RLF2(YPR018W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:ROM2(YLR371W)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL31B(YLR406C)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPL41A(YDL184C)|FD-Score:-3.77|P-value:8.11E-5||SGD DESC:Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication Gene:SCP1(YOR367W)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLT2(YHR030C)|FD-Score:10.5|P-value:5.59E-26||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMA1(YPL027W)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNF4(YGL115W)|FD-Score:-4.72|P-value:1.20E-6||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SPT8(YLR055C)|FD-Score:-4.03|P-value:2.73E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB8(YCR081W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STB5(YHR178W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:STP22(YCL008C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWA2(YDR320C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TOM70(YNL121C)|FD-Score:-3.83|P-value:6.29E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:3.83|P-value:6.54E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.76|P-value:8.55E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.8|P-value:3.24E-9||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP18(YMR071C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMA7(YGR020C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS45(YGL095C)|FD-Score:-3.91|P-value:4.68E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS60(YDR486C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS61(YDR136C_d)|FD-Score:-4.04|P-value:2.67E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAR028W(YAR028W_p)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBR063C(YBR063C_p)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YBR184W(YBR184W_p)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:5.54|P-value:1.48E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEA6(YEL006W)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YET2(YMR040W)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGL260W(YGL260W_p)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR146C-A(YGR146C-A_p)|FD-Score:4.93|P-value:4.10E-7||SGD DESC:Putative protein of unknown function Gene:YGR204C-A(YGR204C-A_p)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR273C(YGR273C_p)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR095W(YHR095W_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:6.75|P-value:7.50E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:-3.9|P-value:4.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKR045C(YKR045C_p)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR036C(YLR036C_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR202C(YLR202C_d)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR349W(YLR349W_d)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YML007C-A(YML007C-A_p)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML054C-A(YML054C-A_p)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Putative protein of unknown function Gene:YMR085W(YMR085W_p)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR122C(YMR122C_d)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL057W(YNL057W_d)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:4.38|P-value:5.83E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YNL115C(YNL115C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YNL143C(YNL143C_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL205C(YNL205C_d)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR065C(YNR065C_p)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR186W(YOR186W_p)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR225W(YOR225W_d)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:7.05|P-value:8.71E-13||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT35(YHR105W)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT52(YKR014C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YPT6(YLR262C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC83(YHR017W)|FD-Score:-4.31|P-value:7.98E-6||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YGL055W22.604.13E-1139.61OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YDR331W13.009.64E-393.07GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YBR140C9.902.12E-231.02IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YHR007C8.883.31E-190.50ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDL196W_d8.382.63E-170.04YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YLR075W8.343.56E-170.02RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YJL143W8.334.14E-170.69TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YBR190W_d7.641.09E-140.15YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YOR232W7.493.47E-140.68MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YGL116W6.814.90E-120.33CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YML105C6.484.65E-110.52SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
YMR203W5.961.29E-90.16TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YOR319W5.793.46E-90.01HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YPL131W5.783.70E-90.84RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YPL093W4.943.93E-70.26NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL226C26.501.70E-154GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YJL204C20.004.44E-89RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YHR030C10.505.59E-26SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YDR127W8.295.70E-17ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YKR026C7.173.86E-13GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YER031C7.058.71E-13YPT31Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
YHR139C-A_d6.757.50E-12YHR139C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR284C6.747.91E-12ERV29Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress
YDR283C6.484.47E-11GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YLR350W6.095.78E-10ORM2Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress
YGL026C5.803.24E-9TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YCR094W5.571.30E-8CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YDR008C_d5.541.48E-8YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YCR024C-A5.472.22E-8PMP1Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
YEL033W_p5.452.49E-8MTC7_pPredicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant

GO enrichment analysis for SGTC_2050
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4840SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.155172fatty acid desaturase (OLE1)
0.4690SGTC_258haloperidol 50.8 μMMiscellaneous35590.140625fatty acid desaturase (OLE1)
0.4650SGTC_2725haloperidol 53.2 μMMiscellaneous35590.140625plasma membrane duress
0.4620SGTC_29939054257 71.4 μMChembridge (Drug-like library)64702170.375fatty acid desaturase (OLE1)
0.4381.39E-275SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.147541fatty acid desaturase (OLE1)
0.4151.30E-244SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.384615plasma membrane duress
0.4011.65E-226SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.375fatty acid desaturase (OLE1)
0.3931.03E-217SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.309091fatty acid desaturase (OLE1)
0.3911.44E-214SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.16plasma membrane duress
0.3887.17E-211SGTC_2733amorolfine 100.0 μMMiscellaneous542600.152542plasma membrane duress
0.3861.08E-208SGTC_28809042983 58.4 μMChembridge (Drug-like library)64647140.285714fatty acid desaturase (OLE1)
0.3834.08E-206SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.224138endomembrane recycling
0.3805.00E-202SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.163636fatty acid desaturase (OLE1)
0.3763.23E-197SGTC_218alverine citrate 93.8 μMMiscellaneous217180.181818fatty acid desaturase (OLE1)
0.3761.16E-197SGTC_11604092-0570 10.5 μMChemDiv (Drug-like library)49949750.258621plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14554262-0120131 μM0.3947371529587ChemDiv (Drug-like library)308.460424.87302plasma membrane duress
SGTC_1832st05411355 μM0.3928571251267TimTec (Natural product derivative library)364.39783.37116
SGTC_6164092-0450108 μM0.391304787165ChemDiv (Drug-like library)322.443943.76202
SGTC_5154092-1064102 μM0.390244717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_2784092-082123.9 μM0.384615877900ChemDiv (Drug-like library)328.87895.05102plasma membrane duress
SGTC_20204017018200 μM0.38461521127491Chembridge (Fragment library)320.36530.23957
SGTC_10194262-02123.4 μM0.3754677213ChemDiv (Drug-like library)352.4881635.20803ergosterol depletion effects on membrane
SGTC_2887904478458.44 μM0.3756465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)
SGTC_2993905425771.43 μM0.3756470217Chembridge (Drug-like library)343.850483.16113fatty acid desaturase (OLE1)
SGTC_3033909088749.47 μM0.37519325384Chembridge (Drug-like library)342.862423.9402fatty acid desaturase (OLE1)