4546-0025

N-[[5-chloro-3-(4-methoxyphenyl)-1-benzofuran-2-yl]methyl]pentan-3-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_206
Screen concentration 72.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6620745
SMILES CCC(CC)NCC1=C(C2=C(O1)C=CC(=C2)Cl)C3=CC=C(C=C3)OC
Standardized SMILES CCC(CC)NCc1oc2ccc(Cl)cc2c1c3ccc(OC)cc3
Molecular weight 357.8738
ALogP 5.69
H-bond donor count 1
H-bond acceptor count 3
Response signature NEO1

Pool Growth Kinetics
% growth inhibition (Het. pool) 36.22
% growth inhibition (Hom. pool) 21.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6620745
Download HIP data (tab-delimited text)  (excel)
Gene:CDC31(YOR257W)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DBP2(YNL112W)|FD-Score:5.3|P-value:5.77E-8|Clearance:0.18||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DOP1(YDR141C)|FD-Score:5.12|P-value:1.54E-7|Clearance:1.03||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERG11(YHR007C)|FD-Score:9.34|P-value:4.78E-21|Clearance:1.26||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:8.08|P-value:3.32E-16|Clearance:2.17||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:5.9|P-value:1.78E-9|Clearance:0.14||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:NEO1(YIL048W)|FD-Score:52.3|P-value:0|Clearance:43.01||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP8(YOL144W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.25||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP85(YJR042W)|FD-Score:-3.12|P-value:8.96E-4|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RPO26(YPR187W)|FD-Score:-3.11|P-value:9.44E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRP43(YCR035C)|FD-Score:4.09|P-value:2.19E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SCC2(YDR180W)|FD-Score:4.04|P-value:2.65E-5|Clearance:0.46||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SEC31(YDL195W)|FD-Score:-3.25|P-value:5.81E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC5(YDR166C)|FD-Score:5.54|P-value:1.54E-8|Clearance:0.24||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SKP1(YDR328C)|FD-Score:4.08|P-value:2.23E-5|Clearance:0.04||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:TOA2(YKL058W)|FD-Score:3.18|P-value:7.33E-4|Clearance:0.14||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YJL086C(YJL086C_d)|FD-Score:5.77|P-value:4.00E-9|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YKL153W(YKL153W_d)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:CDC31(YOR257W)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DBP2(YNL112W)|FD-Score:5.3|P-value:5.77E-8|Clearance:0.18||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DOP1(YDR141C)|FD-Score:5.12|P-value:1.54E-7|Clearance:1.03||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERG11(YHR007C)|FD-Score:9.34|P-value:4.78E-21|Clearance:1.26||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:8.08|P-value:3.32E-16|Clearance:2.17||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:5.9|P-value:1.78E-9|Clearance:0.14||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:NEO1(YIL048W)|FD-Score:52.3|P-value:0|Clearance:43.01||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP8(YOL144W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.25||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP85(YJR042W)|FD-Score:-3.12|P-value:8.96E-4|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RPO26(YPR187W)|FD-Score:-3.11|P-value:9.44E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRP43(YCR035C)|FD-Score:4.09|P-value:2.19E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SCC2(YDR180W)|FD-Score:4.04|P-value:2.65E-5|Clearance:0.46||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SEC31(YDL195W)|FD-Score:-3.25|P-value:5.81E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC5(YDR166C)|FD-Score:5.54|P-value:1.54E-8|Clearance:0.24||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SKP1(YDR328C)|FD-Score:4.08|P-value:2.23E-5|Clearance:0.04||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:TOA2(YKL058W)|FD-Score:3.18|P-value:7.33E-4|Clearance:0.14||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YJL086C(YJL086C_d)|FD-Score:5.77|P-value:4.00E-9|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YKL153W(YKL153W_d)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6620745
Download HOP data (tab-delimited text)  (excel)
Gene:AFG3(YER017C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM26(YKL037W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AKR1(YDR264C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ANP1(YEL036C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:API2(YDR525W_d)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APN1(YKL114C)|FD-Score:6.18|P-value:3.27E-10||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARC18(YLR370C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:BCK1(YJL095W)|FD-Score:7.7|P-value:6.92E-15||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BCK2(YER167W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BFR1(YOR198C)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BSP1(YPR171W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUB3(YOR026W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CCR4(YAL021C)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCW12(YLR110C)|FD-Score:7.76|P-value:4.14E-15||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CIN8(YEL061C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CKB2(YOR039W)|FD-Score:5.58|P-value:1.23E-8||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CSG2(YBR036C)|FD-Score:8.71|P-value:1.52E-18||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:CYS3(YAL012W)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DFG16(YOR030W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:EMC6(YLL014W)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:END3(YNL084C)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERD1(YDR414C)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:FAT1(YBR041W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FUR4(YBR021W)|FD-Score:5.52|P-value:1.67E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GEA2(YEL022W)|FD-Score:5.2|P-value:1.00E-7||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GGA2(YHR108W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GYP1(YOR070C)|FD-Score:7.89|P-value:1.48E-15||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HSP150(YJL159W)|FD-Score:7.71|P-value:6.16E-15||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:IML3(YBR107C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IPT1(YDR072C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KAR9(YPL269W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KCH1(YJR054W_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:KRE1(YNL322C)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KRE2(YDR483W)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LRG1(YDL240W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:LSM1(YJL124C)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MNL2(YLR057W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MNN10(YDR245W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MNN11(YJL183W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPS12(YNR036C)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NAB6(YML117W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NEM1(YHR004C)|FD-Score:4.27|P-value:9.96E-6||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NHX1(YDR456W)|FD-Score:27.6|P-value:1.27E-167||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:OPI3(YJR073C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OPI9(YLR338W_d)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAF1(YBR279W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PAH1(YMR165C)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PAT1(YCR077C)|FD-Score:4.32|P-value:7.67E-6||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PEA2(YER149C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PEP12(YOR036W)|FD-Score:5.04|P-value:2.37E-7||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:6.39|P-value:8.48E-11||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:10.1|P-value:2.65E-24||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:14.1|P-value:1.21E-45||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PER1(YCR044C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PFA4(YOL003C)|FD-Score:4.82|P-value:7.11E-7||SGD DESC:Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases Gene:PHO84(YML123C)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIL1(YGR086C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PMR1(YGL167C)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POP2(YNR052C)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM9(YAR031W)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:RBK1(YCR036W)|FD-Score:4.45|P-value:4.37E-6||SGD DESC:Putative ribokinase Gene:RCY1(YJL204C)|FD-Score:11.9|P-value:4.59E-33||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGL1(YPL066W)|FD-Score:11.5|P-value:1.17E-30||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIM101(YHL027W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:6.99|P-value:1.38E-12||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:-5.99|P-value:1.08E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS10A(YOR293W)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RUD3(YOR216C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:RVS161(YCR009C)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC1(YKL212W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:8.37|P-value:2.94E-17||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM37(YMR060C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAM4(YPL273W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SEC22(YLR268W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEH1(YGL100W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SER3(YER081W)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SHE4(YOR035C)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLA1(YBL007C)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SMI1(YGR229C)|FD-Score:8.26|P-value:7.38E-17||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SMP1(YBR182C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:10.4|P-value:9.72E-26||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:8.26|P-value:7.23E-17||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNX3(YOR357C)|FD-Score:7.63|P-value:1.16E-14||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SPA2(YLL021W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPC2(YML055W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPT10(YJL127C)|FD-Score:-4.69|P-value:1.34E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRN2(YLR119W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SRV2(YNL138W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SSD1(YDR293C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STB5(YHR178W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:STE20(YHL007C)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:STP22(YCL008C)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUR1(YPL057C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SVL3(YPL032C)|FD-Score:-3.92|P-value:4.45E-5||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SXM1(YDR395W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYC1(YOR179C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:TOM7(YNL070W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRP3(YKL211C)|FD-Score:5.39|P-value:3.44E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUS1(YLR425W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBX6(YJL048C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VPS20(YMR077C)|FD-Score:9.75|P-value:9.41E-23||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:8.83|P-value:5.47E-19||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:6.76|P-value:7.00E-12||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS29(YHR012W)|FD-Score:15.1|P-value:1.27E-51||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS3(YDR495C)|FD-Score:4.79|P-value:8.38E-7||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS30(YPL120W)|FD-Score:15.6|P-value:2.11E-55||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS35(YJL154C)|FD-Score:18.6|P-value:3.68E-77||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS36(YLR417W)|FD-Score:12|P-value:2.76E-33||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS38(YLR360W)|FD-Score:8.24|P-value:8.60E-17||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:VPS45(YGL095C)|FD-Score:4.79|P-value:8.14E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:18.1|P-value:1.51E-73||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAP1(YML007W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YCR064C(YCR064C_d)|FD-Score:7.74|P-value:4.93E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL041W(YDL041W_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR455C(YDR455C_d)|FD-Score:29|P-value:9.32E-185||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGR122W(YGR122W)|FD-Score:5.72|P-value:5.27E-9||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIH1(YCR059C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL141W(YIL141W_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL077W(YKL077W_p)|FD-Score:5.8|P-value:3.38E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR111W(YLR111W_d)|FD-Score:15.4|P-value:1.47E-53||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL184C(YNL184C_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL211C(YNL211C_p)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL098C(YOL098C_p)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Putative metalloprotease Gene:YPR172W(YPR172W_p)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:YPR196W(YPR196W_p)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Putative maltose-responsive transcription factor Gene:ZAP1(YJL056C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:AFG3(YER017C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM26(YKL037W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AKR1(YDR264C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ANP1(YEL036C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:API2(YDR525W_d)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APN1(YKL114C)|FD-Score:6.18|P-value:3.27E-10||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARC18(YLR370C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:BCK1(YJL095W)|FD-Score:7.7|P-value:6.92E-15||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BCK2(YER167W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BFR1(YOR198C)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BSP1(YPR171W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUB3(YOR026W)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CCR4(YAL021C)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCW12(YLR110C)|FD-Score:7.76|P-value:4.14E-15||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CIN8(YEL061C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CKB2(YOR039W)|FD-Score:5.58|P-value:1.23E-8||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CSG2(YBR036C)|FD-Score:8.71|P-value:1.52E-18||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:CYS3(YAL012W)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DFG16(YOR030W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:EMC6(YLL014W)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:END3(YNL084C)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERD1(YDR414C)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:FAT1(YBR041W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FUR4(YBR021W)|FD-Score:5.52|P-value:1.67E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GEA2(YEL022W)|FD-Score:5.2|P-value:1.00E-7||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GGA2(YHR108W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GYP1(YOR070C)|FD-Score:7.89|P-value:1.48E-15||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HSP150(YJL159W)|FD-Score:7.71|P-value:6.16E-15||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:IML3(YBR107C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IPT1(YDR072C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KAR9(YPL269W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KCH1(YJR054W_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:KRE1(YNL322C)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KRE2(YDR483W)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LRG1(YDL240W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:LSM1(YJL124C)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MNL2(YLR057W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MNN10(YDR245W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MNN11(YJL183W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPS12(YNR036C)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NAB6(YML117W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NEM1(YHR004C)|FD-Score:4.27|P-value:9.96E-6||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NHX1(YDR456W)|FD-Score:27.6|P-value:1.27E-167||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:OPI3(YJR073C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OPI9(YLR338W_d)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAF1(YBR279W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PAH1(YMR165C)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PAT1(YCR077C)|FD-Score:4.32|P-value:7.67E-6||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PEA2(YER149C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PEP12(YOR036W)|FD-Score:5.04|P-value:2.37E-7||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:6.39|P-value:8.48E-11||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:10.1|P-value:2.65E-24||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:14.1|P-value:1.21E-45||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PER1(YCR044C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PFA4(YOL003C)|FD-Score:4.82|P-value:7.11E-7||SGD DESC:Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases Gene:PHO84(YML123C)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIL1(YGR086C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PMR1(YGL167C)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POP2(YNR052C)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM9(YAR031W)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:RBK1(YCR036W)|FD-Score:4.45|P-value:4.37E-6||SGD DESC:Putative ribokinase Gene:RCY1(YJL204C)|FD-Score:11.9|P-value:4.59E-33||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGL1(YPL066W)|FD-Score:11.5|P-value:1.17E-30||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIM101(YHL027W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:6.99|P-value:1.38E-12||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:-5.99|P-value:1.08E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS10A(YOR293W)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RUD3(YOR216C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:RVS161(YCR009C)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC1(YKL212W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:8.37|P-value:2.94E-17||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM37(YMR060C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAM4(YPL273W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SEC22(YLR268W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEH1(YGL100W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SER3(YER081W)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SHE4(YOR035C)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLA1(YBL007C)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SMI1(YGR229C)|FD-Score:8.26|P-value:7.38E-17||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SMP1(YBR182C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:10.4|P-value:9.72E-26||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:8.26|P-value:7.23E-17||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNX3(YOR357C)|FD-Score:7.63|P-value:1.16E-14||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SPA2(YLL021W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPC2(YML055W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPT10(YJL127C)|FD-Score:-4.69|P-value:1.34E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRN2(YLR119W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SRV2(YNL138W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SSD1(YDR293C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STB5(YHR178W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:STE20(YHL007C)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:STP22(YCL008C)|FD-Score:4.53|P-value:2.91E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUR1(YPL057C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SVL3(YPL032C)|FD-Score:-3.92|P-value:4.45E-5||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SXM1(YDR395W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYC1(YOR179C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:TOM7(YNL070W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRP3(YKL211C)|FD-Score:5.39|P-value:3.44E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUS1(YLR425W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBX6(YJL048C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VPS20(YMR077C)|FD-Score:9.75|P-value:9.41E-23||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:8.83|P-value:5.47E-19||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:6.76|P-value:7.00E-12||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS29(YHR012W)|FD-Score:15.1|P-value:1.27E-51||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS3(YDR495C)|FD-Score:4.79|P-value:8.38E-7||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS30(YPL120W)|FD-Score:15.6|P-value:2.11E-55||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS35(YJL154C)|FD-Score:18.6|P-value:3.68E-77||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS36(YLR417W)|FD-Score:12|P-value:2.76E-33||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS38(YLR360W)|FD-Score:8.24|P-value:8.60E-17||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:VPS45(YGL095C)|FD-Score:4.79|P-value:8.14E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS74(YDR372C)|FD-Score:18.1|P-value:1.51E-73||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAP1(YML007W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YCR064C(YCR064C_d)|FD-Score:7.74|P-value:4.93E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL041W(YDL041W_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR455C(YDR455C_d)|FD-Score:29|P-value:9.32E-185||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGR122W(YGR122W)|FD-Score:5.72|P-value:5.27E-9||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIH1(YCR059C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL141W(YIL141W_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL077W(YKL077W_p)|FD-Score:5.8|P-value:3.38E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR111W(YLR111W_d)|FD-Score:15.4|P-value:1.47E-53||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL184C(YNL184C_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL211C(YNL211C_p)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL098C(YOL098C_p)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Putative metalloprotease Gene:YPR172W(YPR172W_p)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:YPR196W(YPR196W_p)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Putative maltose-responsive transcription factor Gene:ZAP1(YJL056C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W52.30043.00NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YHR007C9.344.78E-211.26ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YGR060W8.083.32E-162.17ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YMR134W_p5.901.78E-90.14ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YJL086C_d5.774.00E-90.23YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YDR166C5.541.54E-80.24SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YNL112W5.305.77E-80.18DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YDR141C5.121.54E-71.03DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YCR035C4.092.19E-50.00RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YDR328C4.082.23E-50.04SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YDR180W4.042.65E-50.46SCC2Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
YOL144W3.581.73E-40.25NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YKL153W_d3.334.36E-40.15YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YKL058W3.187.33E-40.14TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YGR113W3.040.001180.06DAM1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR455C_d29.009.32E-185YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YDR456W27.601.27E-167NHX1Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
YJL154C18.603.68E-77VPS35Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
YDR372C18.101.51E-73VPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
YPL120W15.602.11E-55VPS30Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
YLR111W_d15.401.47E-53YLR111W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YHR012W15.101.27E-51VPS29Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
YJL053W14.101.21E-45PEP8Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
YLR417W12.002.76E-33VPS36Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
YJL204C11.904.59E-33RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YPL066W11.501.17E-30RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YLR025W10.409.72E-26SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YDR323C10.102.65E-24PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YMR077C9.759.41E-23VPS20Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
YJR102C8.835.47E-19VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome

GO enrichment analysis for SGTC_206
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.7010SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.152778NEO1
0.6870SGTC_232clozapine 119.0 μMMiscellaneous28180.131579NEO1
0.6730SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.188406NEO1
0.6320SGTC_2697mebhydrolin 72.4 μMTimTec (Natural product derivative library)225300.0789474NEO1
0.6210SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.181818NEO1
0.6110SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.125NEO1
0.5840SGTC_9001112-0045 141.0 μMChemDiv (Drug-like library)562110.0833333NEO1
0.5780SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.0729167NEO1
0.5530SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0714286NEO1
0.5450SGTC_9403232-1287 54.8 μMChemDiv (Drug-like library)15854930.144578NEO1
0.5430SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.130435NEO1
0.5400SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.188406NEO1
0.5200SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.0963855NEO1-PIK1
0.5180SGTC_221trifluoperazine 10.3 μMMiscellaneous55660.0714286NEO1-PIK1
0.5050SGTC_270tamoxifen 5.5 μMMiscellaneous53760.126761NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1765st04481590.4 μM0.274194645695TimTec (Natural product derivative library)221.209341.51715
SGTC_1929genistein trimethyl ether50.5 μM0.246154136420TimTec (Natural product derivative library)312.316642.81705
SGTC_2886905894451.95 μM0.2461544949929Chembridge (Drug-like library)299.364243.92713
SGTC_2636apigenin dimethyl ether100 μM0.2424245281601Microsource (Natural product library)298.290062.86115
SGTC_3180910928749.47 μM0.24242418891271Chembridge (Drug-like library)311.783862.97214
SGTC_3218913128549.47 μM0.24242417156417Chembridge (Drug-like library)304.1873434.33723endomembrane recycling
SGTC_3084092-1169104 μM0.2388062268590ChemDiv (Drug-like library)346.851123.8514plasma membrane duress
SGTC_13081187-161741.4 μM0.2388062882570ChemDiv (Drug-like library)315.449763.31523fatty acid desaturase (OLE1)
SGTC_2522biochanin a4.94 μM0.2388065280373Microsource (Natural product library)284.263482.36625RNA pol III & RNase P/MRP
SGTC_25903,4',5',6',7'-pentamethoxyflavone100 μM0.235294521171Microsource (Natural product library)372.36862.7580760S ribosome export