5221596

2-sulfanylidene-5-(thiophen-2-ylmethylidene)-1,3-diazinane-4,6-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2065
Screen concentration 34.3 μM
Source Chembridge (Fragment library)
PubChem CID 788581
SMILES C1=CSC(=C1)C=C2C(=O)NC(=S)NC2=O
Standardized SMILES O=C1NC(=S)NC(=O)C1=Cc2cccs2
Molecular weight 238.2861
ALogP 1.72
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.39
% growth inhibition (Hom. pool) 11.27


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 788581
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:6.04|P-value:7.71E-10|Clearance:1.4||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC20(YGL116W)|FD-Score:4.11|P-value:1.97E-5|Clearance:0.11||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CWC22(YGR278W)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.23||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:ESP1(YGR098C)|FD-Score:-3.6|P-value:1.62E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:HEM3(YDL205C)|FD-Score:3.12|P-value:8.89E-4|Clearance:0.01||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:LSM3(YLR438C-A)|FD-Score:4.64|P-value:1.75E-6|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MYO2(YOR326W)|FD-Score:4.54|P-value:2.80E-6|Clearance:0.06||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:PRP22(YER013W)|FD-Score:4.39|P-value:5.61E-6|Clearance:0.28||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RDS3(YPR094W)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.3||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RHO3(YIL118W)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RRP46(YGR095C)|FD-Score:4.49|P-value:3.64E-6|Clearance:0.09||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RSC9(YML127W)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:STH1(YIL126W)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:YDR413C(YDR413C_d)|FD-Score:3.12|P-value:9.19E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YJR141W(YJR141W_p)|FD-Score:4|P-value:3.15E-5|Clearance:0.35||SGD DESC:Essential protein of unknown function Gene:CCT7(YJL111W)|FD-Score:6.04|P-value:7.71E-10|Clearance:1.4||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC20(YGL116W)|FD-Score:4.11|P-value:1.97E-5|Clearance:0.11||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CWC22(YGR278W)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.23||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:ESP1(YGR098C)|FD-Score:-3.6|P-value:1.62E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:HEM3(YDL205C)|FD-Score:3.12|P-value:8.89E-4|Clearance:0.01||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:LSM3(YLR438C-A)|FD-Score:4.64|P-value:1.75E-6|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MYO2(YOR326W)|FD-Score:4.54|P-value:2.80E-6|Clearance:0.06||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:PRP22(YER013W)|FD-Score:4.39|P-value:5.61E-6|Clearance:0.28||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RDS3(YPR094W)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.3||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RHO3(YIL118W)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RRP46(YGR095C)|FD-Score:4.49|P-value:3.64E-6|Clearance:0.09||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RSC9(YML127W)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:STH1(YIL126W)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:YDR413C(YDR413C_d)|FD-Score:3.12|P-value:9.19E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YJR141W(YJR141W_p)|FD-Score:4|P-value:3.15E-5|Clearance:0.35||SGD DESC:Essential protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 788581
Download HOP data (tab-delimited text)  (excel)
Gene:ADP1(YCR011C)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AIM13(YFR011C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIM17(YHL021C)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:APL3(YBL037W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:APN1(YKL114C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARX1(YDR101C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex Gene:ASN1(YPR145W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BCH2(YKR027W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BGL2(YGR282C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BRE5(YNR051C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CKB1(YGL019W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CLA4(YNL298W)|FD-Score:4.96|P-value:3.44E-7||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CMR1(YDL156W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:COT1(YOR316C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:COX18(YGR062C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DIF1(YLR437C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DSF2(YBR007C_p)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECI1(YLR284C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ECM18(YDR125C)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ENT3(YJR125C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:ERI1(YPL096C-A)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FIR1(YER032W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FKH2(YNL068C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FMP48(YGR052W_p)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUS2(YMR232W)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GAS5(YOL030W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall Gene:GZF3(YJL110C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:IRC15(YPL017C)|FD-Score:4.59|P-value:2.17E-6||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:LCB5(YLR260W)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MAL32(YBR299W)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCA1(YOR197W)|FD-Score:6.05|P-value:7.20E-10||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MKC7(YDR144C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MRPS35(YGR165W)|FD-Score:-5.95|P-value:1.33E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NHP6A(YPR052C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NTH2(YBR001C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PHO90(YJL198W)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PST2(YDR032C)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:QRI7(YDL104C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RAD54(YGL163C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RDR1(YOR380W)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RPN4(YDL020C)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SDP1(YIL113W)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SHE10(YGL228W)|FD-Score:-3.19|P-value:6.99E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SKM1(YOL113W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SYF2(YGR129W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRM12(YML005W)|FD-Score:-5.05|P-value:2.26E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRP1(YDR007W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TSC3(YBR058C-A)|FD-Score:5.64|P-value:8.44E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UFO1(YML088W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:UME1(YPL139C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VBA5(YKR105C_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VEL1(YGL258W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS20(YMR077C)|FD-Score:4.6|P-value:2.06E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS64(YDR200C)|FD-Score:-4.39|P-value:5.77E-6||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGL114W(YGL114W_p)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YGR126W(YGR126W_p)|FD-Score:-5.16|P-value:1.24E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR269W(YGR269W_d)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YIL168W(YIL168W)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YKL100C(YKL100C_p)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL222C(YKL222C)|FD-Score:5.28|P-value:6.33E-8||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLL058W(YLL058W_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YML009C-A(YML009C-A_d)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR221C(YMR221C_p)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YMR279C(YMR279C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL205C(YNL205C_d)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR1(YGR281W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPL062W(YPL062W_d)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YSP3(YOR003W)|FD-Score:4.92|P-value:4.35E-7||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ADP1(YCR011C)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AIM13(YFR011C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIM17(YHL021C)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:APL3(YBL037W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:APN1(YKL114C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARX1(YDR101C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex Gene:ASN1(YPR145W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BCH2(YKR027W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BGL2(YGR282C)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BRE5(YNR051C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CKB1(YGL019W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CLA4(YNL298W)|FD-Score:4.96|P-value:3.44E-7||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CMR1(YDL156W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:COT1(YOR316C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:COX18(YGR062C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DIF1(YLR437C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DSF2(YBR007C_p)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ECI1(YLR284C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ECM18(YDR125C)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ENT3(YJR125C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:ERI1(YPL096C-A)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FIR1(YER032W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FKH2(YNL068C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FMP48(YGR052W_p)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUS2(YMR232W)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GAS5(YOL030W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall Gene:GZF3(YJL110C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:IRC15(YPL017C)|FD-Score:4.59|P-value:2.17E-6||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:LCB5(YLR260W)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MAL32(YBR299W)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCA1(YOR197W)|FD-Score:6.05|P-value:7.20E-10||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MKC7(YDR144C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MRPS35(YGR165W)|FD-Score:-5.95|P-value:1.33E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NHP6A(YPR052C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NTH2(YBR001C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PHO90(YJL198W)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PST2(YDR032C)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:QRI7(YDL104C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RAD54(YGL163C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RDR1(YOR380W)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RPN4(YDL020C)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SDP1(YIL113W)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SHE10(YGL228W)|FD-Score:-3.19|P-value:6.99E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SKM1(YOL113W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SYF2(YGR129W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRM12(YML005W)|FD-Score:-5.05|P-value:2.26E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRP1(YDR007W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TSC3(YBR058C-A)|FD-Score:5.64|P-value:8.44E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UFO1(YML088W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:UME1(YPL139C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VBA5(YKR105C_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VEL1(YGL258W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS20(YMR077C)|FD-Score:4.6|P-value:2.06E-6||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS64(YDR200C)|FD-Score:-4.39|P-value:5.77E-6||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YDR034W-B(YDR034W-B_p)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGL114W(YGL114W_p)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters Gene:YGR126W(YGR126W_p)|FD-Score:-5.16|P-value:1.24E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR269W(YGR269W_d)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YIL168W(YIL168W)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YKL100C(YKL100C_p)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL222C(YKL222C)|FD-Score:5.28|P-value:6.33E-8||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLL058W(YLL058W_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YML009C-A(YML009C-A_d)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR221C(YMR221C_p)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YMR279C(YMR279C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL205C(YNL205C_d)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR1(YGR281W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPL062W(YPL062W_d)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YSP3(YOR003W)|FD-Score:4.92|P-value:4.35E-7||SGD DESC:Putative precursor to the subtilisin-like protease III

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YJL111W6.047.71E-101.40CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YLR438C-A4.641.75E-60.10LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YOR326W4.542.80E-60.06MYO2Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
YGR095C4.493.64E-60.09RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YER013W4.395.61E-60.28PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YGL116W4.111.97E-50.11CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YJR141W_p4.003.15E-50.35YJR141W_pEssential protein of unknown function
YPR094W3.651.29E-40.30RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YGR278W3.363.95E-40.23CWC22Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
YDL205C3.128.89E-40.01HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YDR413C_d3.129.19E-40.03YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YGR277C3.080.001030.03CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YPR187W3.050.001130.01RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YDL126C3.040.001170.08CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YDL153C2.970.001510.03SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR197W6.057.20E-10MCA1Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization
YBR058C-A5.648.44E-9TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YKL222C5.286.33E-8YKL222CProtein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
YHL021C5.141.40E-7AIM17Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
YNL298W4.963.44E-7CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YOR003W4.924.35E-7YSP3Putative precursor to the subtilisin-like protease III
YMR077C4.602.06E-6VPS20Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
YMR232W4.602.08E-6FUS2Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
YPL017C4.592.17E-6IRC15Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
YFR011C4.171.52E-5AIM13Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
YGR052W_p4.171.55E-5FMP48_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
YCR011C4.131.84E-5ADP1Putative ATP-dependent permease of the ABC transporter family of proteins
YMR279C4.042.69E-5YMR279CPutative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication
YIL113W4.032.75E-5SDP1Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock
YDL020C3.973.53E-5RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress

GO enrichment analysis for SGTC_2065
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1599.91E-35SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0344828TSC3-RPN4
0.1533.49E-32SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.107143TSC3-RPN4
0.1292.55E-23SGTC_5941487-1584 10.8 μMChemDiv (Drug-like library)50133320.0735294
0.1271.51E-22SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.0857143
0.1247.94E-22SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.084507TSC3-RPN4
0.1232.14E-21SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0714286
0.1232.72E-21SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0769231TSC3-RPN4
0.1193.68E-20SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.115385
0.1181.12E-19SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.109091
0.1181.13E-19SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0857143
0.1171.92E-19SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0980392
0.1163.30E-19SGTC_1738st035555 74.0 μMTimTec (Natural product derivative library)53311400.1TSC3-RPN4
0.1155.62E-19SGTC_1634st006576 41.0 μMTimTec (Natural product derivative library)39153560.075TSC3-RPN4
0.1156.12E-19SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0655738TSC3-RPN4
0.1156.60E-19SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.137255TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21365328288199.3 μM0.75262395Chembridge (Fragment library)222.220540.81724
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.5641031241132ChemDiv (Drug-like library)227.32642.79414
SGTC_23067482397179.52 μM0.44186891448Chembridge (Fragment library)282.702882.18634RPP1 & pyrimidine depletion
SGTC_23276189656200 μM0.4090915349088Chembridge (Fragment library)282.702882.18634
SGTC_22095-(5-methyl-2-thenylidene)-Barbituric acid122.83 μM0.390244616764Chembridge (Fragment library)236.247120.96324
SGTC_8063966-44555.86 μM0.382979714982ChemDiv (Drug-like library)316.3069633.17425
SGTC_2032518511225.99 μM0.3617025718189Chembridge (Fragment library)241.286781.54415
SGTC_2448518511227.17 μM0.3617025718189Chembridge (Fragment library)241.286781.54415
SGTC_5564058-006351.5 μM0.3584916615692ChemDiv (Drug-like library)417.276424.07924
SGTC_5883970-114452.9 μM0.3333331888707ChemDiv (Drug-like library)425.2609434.86425
SGTC_21465545642200 μM0.297872825326Chembridge (Fragment library)202.232441.21713