5231554

2,6-diamino-4-methyl-4H-thiopyran-3,5-dicarbonitrile

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2072
Screen concentration 129.0 μM
Source Chembridge (Fragment library)
PubChem CID 789545
SMILES CC1C(=C(SC(=C1C#N)N)N)C#N
Standardized SMILES CC1C(=C(N)SC(=C1C#N)N)C#N
Molecular weight 192.2409
ALogP 0.43
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.28
% growth inhibition (Hom. pool) 3.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 789545
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:-3.12|P-value:9.15E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ENP2(YGR145W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.02||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:GPI19(YDR437W)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:KRE9(YJL174W)|FD-Score:-3.37|P-value:3.69E-4|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:NOP2(YNL061W)|FD-Score:4.27|P-value:9.82E-6|Clearance:0.46||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPN6(YDL097C)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.01||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPS5(YJR123W)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.11||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RPT4(YOR259C)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.15||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SLD5(YDR489W)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.06||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SPB1(YCL054W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.08||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPC34(YKR037C)|FD-Score:3.12|P-value:8.99E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:STH1(YIL126W)|FD-Score:3.37|P-value:3.69E-4|Clearance:0.14||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUI1(YNL244C)|FD-Score:-3.9|P-value:4.81E-5|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TFC1(YBR123C)|FD-Score:-3.11|P-value:9.24E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UBC9(YDL064W)|FD-Score:-4.06|P-value:2.44E-5|Clearance:0||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YEF3(YLR249W)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YGL069C(YGL069C_d)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.2||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YNL181W(YNL181W_p)|FD-Score:-3.47|P-value:2.65E-4|Clearance:0||SGD DESC:Putative oxidoreductase; required for cell viability Gene:ARP4(YJL081C)|FD-Score:-3.12|P-value:9.15E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ENP2(YGR145W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.02||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:GPI19(YDR437W)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:KRE9(YJL174W)|FD-Score:-3.37|P-value:3.69E-4|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:NOP2(YNL061W)|FD-Score:4.27|P-value:9.82E-6|Clearance:0.46||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPN6(YDL097C)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.01||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPS5(YJR123W)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.11||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RPT4(YOR259C)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.15||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SLD5(YDR489W)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.06||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SPB1(YCL054W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.08||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPC34(YKR037C)|FD-Score:3.12|P-value:8.99E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:STH1(YIL126W)|FD-Score:3.37|P-value:3.69E-4|Clearance:0.14||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUI1(YNL244C)|FD-Score:-3.9|P-value:4.81E-5|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TFC1(YBR123C)|FD-Score:-3.11|P-value:9.24E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UBC9(YDL064W)|FD-Score:-4.06|P-value:2.44E-5|Clearance:0||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YEF3(YLR249W)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YGL069C(YGL069C_d)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.2||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YNL181W(YNL181W_p)|FD-Score:-3.47|P-value:2.65E-4|Clearance:0||SGD DESC:Putative oxidoreductase; required for cell viability

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 789545
Download HOP data (tab-delimited text)  (excel)
Gene:ARF1(YDL192W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:CKI1(YLR133W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CSG2(YBR036C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DUF1(YOL087C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ERP2(YAL007C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FUN26(YAL022C)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GTS1(YGL181W)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HOS3(YPL116W)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:IRC21(YMR073C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KAP123(YER110C)|FD-Score:5.63|P-value:8.89E-9||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MDH1(YKL085W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MED1(YPR070W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAM7(YMR080C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:PBS2(YJL128C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PET123(YOR158W)|FD-Score:4.78|P-value:8.97E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:POF1(YCL047C)|FD-Score:5.13|P-value:1.46E-7||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PRO1(YDR300C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:SAP155(YFR040W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SGT2(YOR007C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SIR1(YKR101W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SPG5(YMR191W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPO23(YBR250W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SWF1(YDR126W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TDH1(YJL052W)|FD-Score:4.38|P-value:5.83E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIM8(YJR135W-A)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TRP4(YDR354W)|FD-Score:-3.85|P-value:5.94E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UPS3(YDR185C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:USV1(YPL230W)|FD-Score:-3.18|P-value:7.46E-4||SGD DESC:Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication Gene:VMR1(YHL035C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YBR226C(YBR226C_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YBR238C(YBR238C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Putative protein of unknown function Gene:YLL058W(YLL058W_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YOL014W(YOL014W_p)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Putative protein of unknown function Gene:YPR027C(YPR027C_p)|FD-Score:5.56|P-value:1.31E-8||SGD DESC:Putative protein of unknown function Gene:YPR050C(YPR050C_d)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:ARF1(YDL192W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:CKI1(YLR133W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CSG2(YBR036C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DUF1(YOL087C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ERP2(YAL007C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FUN26(YAL022C)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GTS1(YGL181W)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:HOS3(YPL116W)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:IRC21(YMR073C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KAP123(YER110C)|FD-Score:5.63|P-value:8.89E-9||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MDH1(YKL085W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MED1(YPR070W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAM7(YMR080C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:PBS2(YJL128C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PET123(YOR158W)|FD-Score:4.78|P-value:8.97E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:POF1(YCL047C)|FD-Score:5.13|P-value:1.46E-7||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PRO1(YDR300C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:SAP155(YFR040W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SGT2(YOR007C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SIR1(YKR101W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SPG5(YMR191W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPO23(YBR250W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SWF1(YDR126W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TDH1(YJL052W)|FD-Score:4.38|P-value:5.83E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIM8(YJR135W-A)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TRP4(YDR354W)|FD-Score:-3.85|P-value:5.94E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UPS3(YDR185C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:USV1(YPL230W)|FD-Score:-3.18|P-value:7.46E-4||SGD DESC:Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication Gene:VMR1(YHL035C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:YBR226C(YBR226C_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YBR238C(YBR238C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Putative protein of unknown function Gene:YLL058W(YLL058W_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YOL014W(YOL014W_p)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Putative protein of unknown function Gene:YPR027C(YPR027C_p)|FD-Score:5.56|P-value:1.31E-8||SGD DESC:Putative protein of unknown function Gene:YPR050C(YPR050C_d)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL061W4.279.82E-60.46NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YGL069C_d3.816.98E-50.20YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YOR259C3.611.54E-40.15RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YDL097C3.462.69E-40.01RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YGR145W3.452.84E-40.02ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YDR489W3.433.01E-40.06SLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YIL126W3.373.69E-40.14STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YJR123W3.246.07E-40.11RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YKR037C3.128.99E-40.00SPC34Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YCL054W3.129.11E-40.08SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YDR113C3.030.001210.06PDS1Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YKL172W2.980.001450.00EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YNL039W2.980.001460.01BDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
YHR036W2.970.001500.03BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YLR378C2.940.001660.02SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL181W5.822.99E-9GTS1Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
YER110C5.638.89E-9KAP123Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
YPR027C_p5.561.31E-8YPR027C_pPutative protein of unknown function
YCL047C5.131.46E-7POF1ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p
YOR158W4.788.97E-7PET123Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
YCR031C4.641.71E-6RPS14AProtein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication
YAL007C4.631.86E-6ERP2Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles
YLR133W4.542.85E-6CKI1Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
YJL052W4.385.83E-6TDH1Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
YCL001W-B_p3.816.91E-5YCL001W-B_pPutative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication
YDL020C3.768.50E-5RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YMR073C3.711.05E-4IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YMR080C3.651.30E-4NAM7ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
YOL014W_p3.631.43E-4YOL014W_pPutative protein of unknown function
YDR126W3.571.79E-4SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion

GO enrichment analysis for SGTC_2072
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1128.09E-18SGTC_20715230951 39.9 μMChembridge (Fragment library)17992720.428571TSC3-RPN4
0.0913.02E-12SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.0307692
0.0889.72E-12SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.0344828SWF1 & branched chain AA biosynthesis
0.0764.57E-9SGTC_1792st045773 43.6 μMTimTec (Natural product derivative library)29192700.0243902
0.0765.20E-9SGTC_1065prochlorperazine maleate 2.5 μMNIH Clinical Collection64360570.0689655
0.0732.42E-8SGTC_6811642-0006 26.6 μMChemDiv (Drug-like library)44465300.0666667
0.0706.18E-8SGTC_21205538279 152.0 μMChembridge (Fragment library)20581970.0416667
0.0699.05E-8SGTC_9841492-0255 2.9 μMChemDiv (Drug-like library)15851980.0188679SWF1 & branched chain AA biosynthesis
0.0681.63E-7SGTC_20625245942 200.0 μMChembridge (Fragment library)13761600.0408163
0.0681.97E-7SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.0416667superoxide
0.0655.72E-7SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0208333
0.0648.23E-7SGTC_2695st077686 52.0 μMTimTec (Natural product derivative library)28760920.0416667
0.0649.25E-7SGTC_28739040596 71.4 μMChembridge (Drug-like library)64638630.030303
0.0631.23E-6SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0666667
0.0631.30E-6SGTC_488nitrendipine 139.0 μMMiscellaneous45070.046875

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20795212404121 μM0.551724758462Chembridge (Fragment library)220.294081.34325
SGTC_20865274770176.62 μM0.483871689278Chembridge (Fragment library)254.31031.46325
SGTC_21125488546200 μM0.483871600757Chembridge (Fragment library)260.357942.13625TSC3-RPN4
SGTC_2084530607860.64 μM0.457143679340Chembridge (Fragment library)258.2990.79326
SGTC_2092530369480.67 μM0.4571431963699Chembridge (Fragment library)274.36461.35126TSC3-RPN4
SGTC_21115486399200 μM0.457143689674Chembridge (Fragment library)282.363462.40525
SGTC_20785212016200 μM0.4545451899708Chembridge (Fragment library)288.755362.1272560S ribosome export
SGTC_20435101342149 μM0.444444678451Chembridge (Fragment library)282.363462.43525SWF1 & branched chain AA biosynthesis
SGTC_20905301565104.45 μM0.4444442056344Chembridge (Fragment library)274.36461.69226TSC3-RPN4
SGTC_21135491891107.03 μM0.441176789979Chembridge (Fragment library)268.336881.91925TSC3-RPN4